Abstract:
:We have previously described a revertant cell line which expresses a dominant tumor suppressor phenotype to E1 but not to heterologous oncogenes such as c-myc, N-ras, or polyoma middle t (Sircar et al. (1988) Oncogene 3, 725-728). DNA tumor virus oncogenes have been suggested to transform cells via the common mechanism of sequestering the Rb-105 antioncoprotein. This paradigm would predict that our revertant cell line, which is resistant to retransformation by E1a, should also be resistant to the other members of the Rb-105 binding family of oncogenes. To test this hypothesis we transfected the revertant cell line with plasmids bearing SV40-T or the HPV16-E7 oncogenes. Because transformation was obtained by both oncogenes at efficiencies similar to the transformation of a related revertant cell line, the results suggest that the resistance phenotype is specific to E1a. This specificity was further confirmed by cell fusion experiments.
journal_name
Virologyjournal_title
Virologyauthors
Sircar S,Horvath J,Roberge D,Diouri M,Weber JMdoi
10.1016/0042-6822(92)90180-wsubject
Has Abstractpub_date
1992-11-01 00:00:00pages
187-92issue
1eissn
0042-6822issn
1096-0341journal_volume
191pub_type
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