Abstract:
:Within immunocompromised populations, the JC polyomavirus is the cause of the often-fatal disease Progressive Multifocal Leukoencephalopathy (PML). JC virus encodes a protein, termed T-antigen (T-ag), which is essential for its replication and pathogenicity. Previous studies of JCV T-ag have, in general, used antibodies raised against SV40 T-ag. Unfortunately, SV40 T-ag is also detected in humans and therefore there have been concerns about cross-reactivity. To address this issue, we have isolated a monoclonal antibody that binds to the JCV, but not the SV40, T-ag origin-binding domain (OBD). Furthermore, the region on the surface of the JCV T-ag OBD that is recognized by the "anti-JCV OBD mAb" has been mapped. We also demonstrate that the "anti-JCV OBD mAb" will be a useful reagent for standard techniques (e.g., Westerns blots and ELISAs). Finally, we note that additional monoclonal Abs that are specific for the T-ags encoded by the other human polyomaviruses could be generated by adopting the approach described herein.
journal_name
Virologyjournal_title
Virologyauthors
Grubman SA,Shin J,Phelan PJ,Gong A,Can H,Dilworth R,Kini SK,Gagnon D,Archambault J,Meinke G,Bohm A,Jefferson DM,Bullock PAdoi
10.1016/j.virol.2016.07.006subject
Has Abstractpub_date
2016-10-01 00:00:00pages
92-101eissn
0042-6822issn
1096-0341pii
S0042-6822(16)30173-8journal_volume
497pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::A nonaphid transmissible (NAT) variant of tobacco vein mottling virus (TVMV) was used to test the hypothesis that the viral coat protein (CP) plays a role in determining aphid transmissibility. Comparison of the nucleotide sequences in the coat protein cistron of an aphid transmissible isolate (TVMV-AT) with that of T...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90389-9
更新日期:1990-09-01 00:00:00
abstract::The nucleotide sequences of the long terminal repeat (LTR) of simian retroviruses (STLV), which are closely related to human T-cell leukemia virus type I (HTLV-I) were found to be of at least two subgroups: an Asian subtype in macaques and an African subtype in African green monkeys and chimpanzee. The nucleotide sequ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90336-3
更新日期:1986-01-30 00:00:00
abstract::Mouse hepatitis viruses (MHV) of different virulence for mice were studied with respect to interferon (IFN) sensitivity. The growth of low-virulent MHV-S and intermediately virulent MHV-JHM was significantly suppressed in IFN-treated L cells compared with untreated cells. However, a comparable suppression of the growt...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90225-9
更新日期:1985-11-01 00:00:00
abstract::In transient gene expression assays we observed an increase in expression of the bacterial chloramphenicol acetyl-transferase (CAT) gene, under the transcriptional control of the HIV-1 LTR (pLTR-CAT), when this plasmid was cotransfected into Vero or MRC-5 cells with a plasmid containing either the HCMV immediate early...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90233-h
更新日期:1990-05-01 00:00:00
abstract::Several purified species of human leukocyte interferon, including recombinant interferons, inhibit the multiplication of tobacco mosaic virus (TMV) in tobacco protoplasts derived from various cultivars. Viral RNA accumulation was determined by dot-blot hybridization to specific cDNA probes, and virus antigen was deter...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90456-8
更新日期:1985-01-15 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) displays notorious genetic, antigenic, and clinical variability. Little is known, however, about the nature and extent of viral variation present within naturally infected animals. By amplifying and cloning the open reading frame 5 gene from tonsils of natura...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.07.009
更新日期:2003-12-20 00:00:00
abstract::Nipah virus (NiV) causes severe encephalitis in humans, with high mortality. NiV nonstructural C protein (NiV-C) is essential for its pathogenicity, but its functions are unclear. In this study, we focused on NiV-C trafficking in cells and found that it localizes predominantly in the cytoplasm but partly in the nucleu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.07.013
更新日期:2016-10-01 00:00:00
abstract::Virus infection may contribute to asthma pathogenesis. In turn, a Th2-polarized pulmonary environment may increase host susceptibility to infection. We used a cockroach antigen (CRA) model of allergic airway disease to test the hypothesis that Th2 cytokine overproduction increases susceptibility to mouse adenovirus ty...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.06.009
更新日期:2009-08-15 00:00:00
abstract::A short upstream open reading frame (uORF2) in the human cytomegalovirus (CMV) gpUL4 (gp48) transcript leader is conserved among CMV strains and inhibits translation of a downstream cistron. Remarkably, this inhibitory effect depends on the amino acid coding information of uORF2, at least in transient transfection ass...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1630
更新日期:1994-11-15 00:00:00
abstract::The HIV-1 Gag polyprotein specifically incorporates the cellular peptidylprolyl isomerase cyclophilin A into virions. HIV-1 replication is inhibited by cyclosporine A, an immunosuppressive drug which binds with high affinity to cyclophilin A and precludes interaction with the Gag polyprotein. Using a panel of four dru...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0421
更新日期:1996-08-01 00:00:00
abstract::To investigate differential evolutionary rates and selective forces of WNV in hosts and vectors, we measured the genetic diversity that arose during alternating passage in mosquitoes and birds. Within-host genetic diversity was monitored in each of three experimentally passed replicates, and the complete genome sequen...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.02.032
更新日期:2008-05-10 00:00:00
abstract::In reporter-based transient expression systems, we characterized simian immunodeficiency virus from a chimpanzee (SIVCPZ), with special reference to the human immunodeficiency virus type 1 (HIV-1). SIVCPZ was not equally activated by tat and rev transactivators derived from representative primate lentiviruses. HIV-1 a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90715-2
更新日期:1992-07-01 00:00:00
abstract::The quasispecies structure of nervous necrosis virus (NNV) was determined to investigate an outbreak of viral nervous necrosis disease at a barramundi (Lates calcarifer) hatchery. A traditional epidemiological investigation indicated horizontal transmission of infection between two cohorts of fish. However, variation ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.10.011
更新日期:2013-02-05 00:00:00
abstract::Altered cytokine profiles and imbalanced frequencies of CD4(+) T helper cell subsets are thought to be linked with HIV-1/AIDS pathogenesis, but the causes need to be further clarified. Histamine, a biogenic amine with many functions, shows enhancement in HIV-1 infected individuals, which are considered to link with di...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.04.009
更新日期:2013-08-01 00:00:00
abstract::The monocytoid tumor cell line U-937 and five derived subclones were infected with the HTLV-IIIB isolate of the human immunodeficiency virus (HIV). Susceptibility to infection and sensitivity to the cytopathic effects correlated with the expression of the T4 antigen on the cell surface. On the basis of these character...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90278-9
更新日期:1987-04-01 00:00:00
abstract::The pathogenesis of Venezuelan equine encephalitis virus (VEE) was examined in the mouse model using V3000, a virus derived from a molecular clone of the Trinidad donkey strain of VEE. These results were compared in parallel experiments with avirulent mutants of VEE derived by site-directed mutagenesis of the clone. A...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1022
更新日期:1995-02-01 00:00:00
abstract::Lymphocytic choriomeningitis virus (LCMV) Armstrong caused disordered growth and glucose metabolism secondary to growth hormone deficiency in infected C3H/St mice. In contrast, LCMV strain WE replicated in fewer growth hormone-producing cells, failed to disrupt growth hormone synthesis, and did not cause growth hormon...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90431-3
更新日期:1985-04-15 00:00:00
abstract::An effective type I interferon (IFN-alpha/beta) response is critical for the control of many viral infections. Using an oncolytic strain of vesicular stomatitis virus, we have examined the cross-talk between virus-induced apoptosis and initiation of innate immune response. The intrinsic apoptotic cascade, specifically...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.03.011
更新日期:2007-08-15 00:00:00
abstract::Cellular activation plays an important role in retroviral replication. Previously, we have shown that CD4(+)CD25(+) T cells by the virtue of their partially activated phenotype represent ideal candidates for a productive feline immunodeficiency virus (FIV) infection. In the present study, we extended our previous obse...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.02.016
更新日期:2005-05-10 00:00:00
abstract::Varicella-zoster virus (VZV) open reading frame 61 (ORF61) protein transactivates expression of VZV promoters. VZV ORF61 is functionally homologous to herpes simplex virus type 1 (HSV-1)-infected cell protein 0 (ICP0); however, amino acid sequence homology of these two proteins is limited to the RING finger domains, a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1639
更新日期:1994-11-15 00:00:00
abstract::Three proteins, reacting specifically with sera raised against synthetic peptides identical to C-terminal amino acid sequences in alfalfa mosaic virus (AIMV) proteins P1, P2, and P3 translated in vitro from the AIMV RNAs 1, 2, and 3, respectively, were for the first time observed in tobacco and cowpea protoplasts. Par...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90185-1
更新日期:1987-11-01 00:00:00
abstract::Dengue 2 (DEN 2) virus strains collected from dengue hemorrhagic fever (DHF) patients and Aedes aegypti mosquitoes in Thailand, Burma, and Vietnam over a 25-year period have been analyzed by computer assisted T1-RNase-resistant oligonucleotide fingerprinting. Fifty-seven DEN 2 virus strains of the Thailand topotype we...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90195-5
更新日期:1989-10-01 00:00:00
abstract::We have used cells infected with the HIV-1 molecular clone HX10 to study the binding of monoclonal antibodies (mAbs) to different epitopes within the extracellular domain of the HIV-1 transmembrane glycoprotein gp41. Gp41 mAb binding to the infected cells at 4 degrees was variable but weaker than the binding of an ant...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(95)80094-8
更新日期:1995-01-10 00:00:00
abstract::The behavior of two genetically different molecular clones of visna virus KV1772-kv72/67 and LV1-1KS1 was compared in vivo and in vitro. On intracerebral inoculation, clone KV1772-kv72/67 induced a similar response in five sheep as has already been reported with neurovirulent derivates of visna virus. Virus was freque...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.8428
更新日期:1997-03-17 00:00:00
abstract::Nineteen independent temperature-sensitive (ts) mutants were isolated from SA11 following mutagenesis with proflavin or 5-azacytidine. Fourteen of the ts mutants fell into one or another of five mutant groups previously defined by recombination. Five of the ts mutants defined five recombination groups (F, G, H, I, and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90100-9
更新日期:1983-10-30 00:00:00
abstract::We have used a yeast two-hybrid system to identify a baculoviral gene encoding a protein kinase-interacting protein (PKIP). The pkip gene is located at 15.8-16.2 m.u (map units) and represents ORF 24 in the sequence of Ayres et al. (1994) of the Autographa californica nuclear polyhedrosis virus. PKIP is a 19.2-kDa pro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8944
更新日期:1998-01-20 00:00:00
abstract::All primates studied to date produce retroviral-like particles in their placentae. We have purified these particles from two primate species, one Old World (human) and one New World (marmoset), and have identified the retroviral sequences which are packaged into these particles. Three families of sequences have been d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0443
更新日期:1996-08-15 00:00:00
abstract::Viral vector particles derived from murine leukemia virus (MLV) mediate highly efficient stable gene transfer used in gene therapeutic approaches and in the generation of transgenic cell lines. However, the establishment of stable viral packaging cells (VPCs) is a time-consuming challenge. To overcome this limitation,...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.02.014
更新日期:2019-05-01 00:00:00
abstract::A critical feature of highly pathogenic avian influenza viruses (H5N1 and H7N7) is the efficient intracellular cleavage of the hemagglutinin (HA) protein. H7N7 viruses also exist in equine species, and a unique feature of the equine H7N7 HA is the presence of an eleven amino acid insertion directly N-terminal to a tet...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.09.004
更新日期:2012-12-05 00:00:00
abstract::The Pr76Gag and Pr180Gag-Pol polyprotein precursors of Rous sarcoma virus contain a 22-amino-acid spacer peptide, called p2, located between the amino acid sequences of the mature Gag proteins MA and p10. This spacer peptide is present in stoichiometric amounts in the virion, albeit cleaved into two parts, but its fun...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1450
更新日期:1994-08-15 00:00:00