Abstract:
:Varicella-zoster virus (VZV) open reading frame 61 (ORF61) protein transactivates expression of VZV promoters. VZV ORF61 is functionally homologous to herpes simplex virus type 1 (HSV-1)-infected cell protein 0 (ICP0); however, amino acid sequence homology of these two proteins is limited to the RING finger domains, a recently identified sequence motif composed of cysteine and histidine residues, located in their amino-terminal regions. A carboxy-terminal truncation mutant of ICP0 (which retains the RING finger domain) was previously shown to act as a potent transrepressor and as a dominant-negative mutant in the presence of full-length ICP0. We have shown that the corresponding region of ORF61 has similar properties. Amino-terminal truncation mutants of VZV ORF61 and HSV-1 ICP0, which lack the RING finger domain, cannot transactivate VZV promoters; however, fusion of the amino portion of one protein to the carboxy portion of the other restored the transactivating activity of the full-length proteins. To further study the role of the ORF61 RING finger domain, cysteine or histidine residues of this domain were individually replaced with serine or aspargine, respectively. Each of these amino acid substitution mutants in the RING finger abolished the transactivating activity of the full-length ORF61 protein. Each of the substitutions also reduced the transrepressing and dominant-negative properties of a carboxy-terminal truncation mutant of ORF61. These results indicate that the RING finger domain is required for the transregulatory functions of ORF61.
journal_name
Virologyjournal_title
Virologyauthors
Moriuchi H,Moriuchi M,Cohen JIdoi
10.1006/viro.1994.1639subject
Has Abstractpub_date
1994-11-15 00:00:00pages
238-46issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(84)71639-4journal_volume
205pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The Rice stripe virus (RSV) pc4 has been determined as the viral movement protein (MP). In this study, the pc4 gene was cloned into a movement-deficient Tobacco mosaic virus (TMV). The resulting hybrid TMV-pc4, in addition to spreading cell to cell in Nicotiana tabacum, moved systemically and induced foliar necrosis i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.01.007
更新日期:2012-04-10 00:00:00
abstract::It is well established that the human subgroup C adenovirus type 5 (Ad5) E1B 55 kDa protein can regulate the activity and concentration of the cellular tumor suppressor, p53. However, the contribution(s) of these functions of the E1B protein to viral reproduction remains unclear. To investigate this issue, we examined...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.016
更新日期:2008-09-01 00:00:00
abstract::Progressive reorganization and depolymerization of microtubules corresponded with virus-induced rounding of Autographa californica M nuclear polyhedrosis virus (AcMNPV)-infected Spodoptera frugiperda IPLB-Sf-21 cells, suggesting that microtubules were instrumental in maintaining the normal shape of these cells. Depoly...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90211-9
更新日期:1990-03-01 00:00:00
abstract::The utilization of transgenic plants expressing recombinant antigens to be used in the formulation of experimental immunogens has been recently communicated. We report here the development of transgenic plants of alfalfa expressing the structural protein VP1 of foot and mouth disease virus (FMDV). The presence of the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9590
更新日期:1999-03-15 00:00:00
abstract::A new set of lambdoid phages (mEp) classified into different immunity groups was previously described. Phages mEp213, mEp237, and mEp410 were unable to grow in mEp167 lysogenic cells, presumably due to an exclusion mechanism expressed constitutively by the mEp167 repressed prophage. In this work, to analyze the exclus...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.09.005
更新日期:2004-11-24 00:00:00
abstract::The amino acid sequence of the coat protein of wild-type cowpea chlorotic mottle virus has been nearly completely determined by direct methods. The sites of amino acid replacements were identified in the coat proteins of an oxidation-sensitive mutant, a temperature-sensitive mutant, and a salt-stable mutant. The repla...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90108-8
更新日期:1982-06-01 00:00:00
abstract::JC virus (JCV) Agnoprotein (Agno) plays critical roles in successful completion of the viral replication cycle. Understanding its regulatory roles requires a complete map of JCV-host protein interactions. Here, we report the first Agno interactome with host cellular targets utilizing "Two-Strep-Tag" affinity purificat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.10.005
更新日期:2020-01-15 00:00:00
abstract::The primary cell types that sustain polyomavirus (Py) replication following intranasal infection as well as the nature of the host cellular response to Py were unknown. As this is an essential and specific site for virus entry, it seems likely that viral gene function must be adapted to these mucosal tissues. Using im...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0030
更新日期:2000-01-05 00:00:00
abstract::Sixteen monoclonal antibodies directed to the hemagglutinin-neuraminidase (HN) protein of a 1957 isolate of parainfluenza type 3 virus (PIV3) were produced and used to examine antigenic variation in clinical strains. Analysis of hemagglutination-inhibition reactivity patterns of antigenic variants selected in vitro in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90395-2
更新日期:1985-06-01 00:00:00
abstract::The expression of cytokines may influence the development of lymphoma in retrovirally infected animals in at least two ways: (1) cytokines in the tumor environment may stimulate the proliferation of tumor cells and/or (2) cytokines in the tumor environment may diminish the cell-mediated antitumor immune response. To e...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9846
更新日期:1999-09-01 00:00:00
abstract::The minute virus of mice, prototype strain (MVMp), is a non-enveloped, single-stranded DNA virus of the family Parvoviridae. Unlike other parvoviruses, the mechanism of cellular uptake of MVMp has not been studied in detail. We analyzed MVMp endocytosis in mouse LA9 fibroblasts and a tumor cell line derived from epith...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.02.054
更新日期:2015-08-01 00:00:00
abstract::V-CATH, a cathepsin L-like cysteine protease encoded by the baculovirus Autographa californica M nucleopolyhedrovirus, has been shown to play an essential role in host liquefaction. Similar to cellular cathepsin L, V-CATH is synthesized as an inactive proenzyme and is activated by cleavage of the propeptide. Previous ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1378
更新日期:2002-05-10 00:00:00
abstract::Here, we evaluated the role of two host proteins, a Bromo domain containing RNA binding protein (BRP1) and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), in the replication of Cucumber mosaic virus (CMV). LC-MS/MS analysis of host/viral proteins pull down against BRP1 from CMV-infected plants co-infiltrated with BR...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.04.001
更新日期:2016-07-01 00:00:00
abstract::Dengue virus (DENV) is an arbovirus that belongs to the Flaviviridae family. Studies reveal that peptides secreted by amphibians have many functions, such as antiviral and antimicrobial activities. As there is no antiviral drug effective against the DENV, the antiviral activity of a synthetic peptide called HS-1, deri...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.11.009
更新日期:2018-01-15 00:00:00
abstract::The envelope glycoprotein (Env) of Moloney murine leukemia virus (MoMuLV) is proteolytically processed and transported to the cell surface where it can be incorporated into budding virions. Cell surface Env is frequently detected using an indirect immunofluorescence assay and fluorescence-activated cell sorting (FACS)...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9073
更新日期:1998-04-10 00:00:00
abstract::Full-length cDNA clones of RNA 2 of tobacco rattle virus (TRV) strain PLB have been cloned into the transcription vector pPM1. Products of in vitro transcription by Escherichia coli RNA polymerase, either capped or uncapped, were as infectious as native RNA 2 when coinoculated with RNA 1 of TRV strain TCM. At least 70...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90222-5
更新日期:1989-11-01 00:00:00
abstract::Cardioviruses cause diseases in many animals including, in rare cases, humans. Although they share common features with all picornaviruses, cardioviruses have unique properties that distinguish them from other family members, including enteroviruses. One feature shared by all picornaviruses is the covalent attachment ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.01.010
更新日期:2018-03-01 00:00:00
abstract::CD8 T cells contribute to effective clearance of mouse adenovirus type 1 (MAV-1) and to virus-induced pulmonary inflammation. We characterized effects of a CD8 T cell effector, TNF, on MAV-1 pathogenesis. TNF inhibited MAV-1 replication in vitro. TNF deficiency or immunoneutralization had no effect on lung viral loads...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.05.004
更新日期:2020-08-01 00:00:00
abstract::Several strains of tobacco ringspot virus (TobRV) support the replication and encapsidation of satellite tobacco ringspot virus RNA (STobRV RNA). We have compared the nucleotide sequences of four STobRV RNAs, each initially associated with a different isolate of TobRV. A STobRV RNA from a geranium isolate of TobRV and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90049-3
更新日期:1987-09-01 00:00:00
abstract::Avian influenza A viruses of the H5 and H7 subtypes periodically cause severe outbreaks of disease in poultry. The question we wished to address in this study is whether these highly pathogenic strains constitute unique lineages or whether they and related nonpathogenic viruses are derived from common ancestors in the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1301
更新日期:1995-06-01 00:00:00
abstract::Powassan virus (POWV, Flaviviridae: Flavivirus) is the sole North American member of the tick-borne encephalitis complex and consists of two distinct lineages that are maintained in ecologically discrete enzootic transmission cycles. The underlying genetic mechanisms that lead to niche partitioning in arboviruses are ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.03.035
更新日期:2010-07-05 00:00:00
abstract::Tetherin (ST2/CD317) is a cellular protein that restricts the release from cells of some enveloped viruses including HIV-1. To examine if influenza virus is affected by tetherin, MDCK cells constitutively expressing human tetherin and control MDCK cells were infected with influenza virus. No difference was observed in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.05.006
更新日期:2011-08-15 00:00:00
abstract::Recently, we reported that in vitro and in vivo persistent infection of neurons by lymphocytic choriomeningitis virus (LCMV) downregulated GAP43 expression, a protein involved in neuronal plasticity associated with learning and memory. Here, we investigated the transcriptional and posttranscriptional events involved. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8458
更新日期:1997-04-14 00:00:00
abstract::Varicella zoster virus (VZV) is a neurotropic alphaherpesvirus that, following primary infection (varicella), establishes latency in sensory, autonomic, sympathetic and parasympathetic neurons, where it remains until reactivation (zoster). VZV-specific cell-mediated immune responses maintain VZV latency; thus, immunos...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.06.013
更新日期:2018-09-01 00:00:00
abstract::Genetic variation of the Bovine Leukemia Virus (BLV) appears to be limited in vitro and during the latent phase of the disease. However, cells in tumors often harbor deleted proviruses that are defective for expression. In order to gain insight into the involvement of viral genetic variation during pathogenesis, the B...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(95)80007-7
更新日期:1995-01-10 00:00:00
abstract::The coding region of segment 4 of Thogoto (THO) virus, a tick-borne member of the Orthomyxoviridae, was expressed in a baculovirus system under the control of the polyhedrin promoter. This construct expressed authentic envelope glycoprotein as determined by size and antigenic reactivity with a panel of monoclonal anti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1566
更新日期:1995-10-20 00:00:00
abstract::The measles virus (MV) vaccine lineage is a promising oncolytic but prior exposure to the measles vaccine or wild-type MV strains limits treatment utility due to the presence of anti-measles antibodies. MV entry can be redirected by displaying a polypeptide ligand on the Hemagglutinin (H) C-terminus. We hypothesized t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.01.027
更新日期:2014-04-01 00:00:00
abstract::The presence of anti-Tax antibody responses in human T cell leukemia virus type I (HTLV-I)-infected individuals has been correlated with increased proviral load, increased risk of transmitting infection, and increased risk of developing tropical spastic paraparesis/HTLV-I-associated myelopathy (TSP/HAM). In this study...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9378
更新日期:1998-10-10 00:00:00
abstract::While trying to generate a site-directed deletion in the ORF63 latency-associated gene of varicella-zoster virus (VZV) Oka, we constructed a virus with an unexpected rearrangement. The virus has a small deletion in both copies of ORF63 and two copies of a cassette inserted between ORFs 64/65 and 68/69 containing (a) t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.01.007
更新日期:2010-04-10 00:00:00
abstract::Old world hantaviruses, causing hemorrhagic fever with renal syndrome (HFRS), still present a public health problem in Asia and Eastern Europe. The majority of cases has been recorded in China. The aim of our study was to generate human recombinant neutralizing antibodies to a hantavirus by phage display technology. T...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(02)00094-6
更新日期:2003-03-30 00:00:00