Quasispecies variation of porcine reproductive and respiratory syndrome virus during natural infection.

abstract：:We have cloned an infectious genome of the Junonia coenia densonucleosis virus (JcDNV) into the bacterial plasmid pBR322. The viral genome could be rescued from the recombinant plasmid pBRJ by transfection of pBRJ DNA to sensitive Spodoptera littoralis larvae. pBRJ DNA produced a typical viral infection and a comparab...

journal_title：Virology

authors： Jourdan M,Jousset FX,Gervais M,Skory S,Bergoin M,Dumas B

更新日期：1990-11-01 00:00:00

abstract：:A mutant strain of polyoma virus encoding a truncated middle T protein has been studied for its ability to replicate and induce tumors following inoculation into newborn mice. Virus replication in the acute period prior to expected onset of tumors as well as persistence of virus in older animals were followed. The mut...

journal_title：Virology

authors： Freund R,Sotnikov A,Bronson RT,Benjamin TL

更新日期：1992-12-01 00:00:00

abstract：:Recently, we identified TT virus (TTV) isolates from nonhuman primates and named them simian TTV (s-TTV). To characterize the genomic structure of these isolates in more detail, the full-length nucleotide sequence of the s-TTV isolate (designated s-TTV CH65-1), recovered from a chimpanzee born in West Africa, was ampl...

journal_title：Virology

authors： Inami T,Obara T,Moriyama M,Arakawa Y,Abe K

更新日期：2000-11-25 00:00:00

abstract：:An antiserum was raised against a synthetic peptide corresponding to the 18 C-terminal amino acids of a putative 16K protein encoded by the 3'-terminal open reading frame of tobacco rattle virus (TRV) RNA-1. This antiserum was used to demonstrate expression of the 16K cistron in vivo. TRV-infected tobacco protoplasts ...

journal_title：Virology

authors： Angenent GC,Verbeek HB,Bol JF

更新日期：1989-04-01 00:00:00

abstract：:Mouse hepatitis virus (MHV) JHMV and its soluble receptor-resistant (srr) mutants, srr7, srr11, and srr18, grew and induced syncytia equally well in BHK-R1 cells expressing the MHVR1 receptor derived from MHV-susceptible BALB/c mice. In contrast, srr growth and syncytia formations were drastically reduced relative to ...

journal_title：Virology

authors： Matsuyama S,Taguchi F

更新日期：2000-07-20 00:00:00

abstract：:The location of the cistron encoding the genome-linked protein (VPg) in the potyvirus tobacco vein mottling virus (TVMV) was investigated. Precipitation of 125I-labeled VPg with anti-tobacco etch virus 49K nuclear inclusion protein antiserum (which reacts with the NIa nuclear inclusion protein of TVMV) indicated that ...

journal_title：Virology

authors： Shahabuddin M,Shaw JG,Rhoads RE

更新日期：1988-04-01 00:00:00

abstract：:Human parainfluenza virus type 2 phosphoprotein (P) is an essential component of viral polymerase. The P gene encodes both P and accessory V proteins by a specific gene editing mechanism. Therefore, the N-terminal 164 amino acids of P protein are common to V protein. Interestingly, while P protein is located in the cy...

journal_title：Virology

authors： Ohtsuka J,Matsumoto Y,Ohta K,Fukumura M,Tsurudome M,Nosaka T,Nishio M

更新日期：2019-02-01 00:00:00

abstract：:To study the possible role of immune selection in the in vivo generation of pathogenic recombinant murine leukemia viruses (MuLV), we have constructed recombinant vaccinia viruses (rVV) expressing the envelope genes of three MuLV: AKR623, MCF247, and MCF13. rVV expressing either AKR623 or MCF247 env could prime H-2b m...

journal_title：Virology

authors： Coppola MA,Green WR

更新日期：1994-07-01 00:00:00

abstract：:Human embryo lung cells pretreated with a combination of human leukocyte interferon and acyclovir were infected with human cytomegalovirus (HCMV) and treated daily for 14 days with the same inhibitor combination. After removal of the inhibitors, the incubation temperature was maintained at either 37 or 40.5 degrees. I...

journal_title：Virology

authors： Bucher LJ,Wigdahl B,Rapp F

更新日期：1983-10-15 00:00:00

abstract：:Hepatitis C virus (HCV) is efficient in the establishment of persistent infection. We have previously shown that HCV core protein inhibits T cell proliferation through its interaction with the complement receptor, gC1qR. Here we show that HCV core-induced inhibition of T cell proliferation involves a G(0)/G(1) cell cy...

journal_title：Virology

authors： Yao ZQ,Eisen-Vandervelde A,Ray S,Hahn YS

更新日期：2003-09-15 00:00:00

abstract：:We reported previously that carbohydrate attachment to an overlapping glycosylation site adjacent to the signal peptide of GP3 from equine arteritis virus (EAV) prevents cleavage. Here we investigated whether this unusual processing scheme is a feature of GP3s of other Arteriviridae, which all contain a glycosylation ...

journal_title：Virology

authors： Zhang M,Veit M

更新日期：2018-04-01 00:00:00

abstract：:The complete genomic RNAs of two Korean sacbrood virus (SBV) strains, which infect the honey bee, Apis mellifera, were sequenced. The two sequences (AmSBV-Kor19, AmSBV-Kor21) were distinguished by the presence or absence of a PstI restriction site. These strains comprised of 8784 bp and 8835 bp; contained a single lar...

journal_title：Virology

authors： Choe SE,Nguyen LT,Noh JH,Kweon CH,Reddy KE,Koh HB,Chang KY,Kang SW

更新日期：2012-10-10 00:00:00

abstract：:In benign squamous lesions and in organotypic epithelial cultures, the human papillomavirus (HPV) E6 and E7 genes are transcriptionally up-regulated in differentiated, spinous keratinocytes. We previously identified sequence elements in the enhancer-promoter regions of HPV types 18 and 11 important for this promoter r...

journal_title：Virology

authors： Zhao W,Chow LT,Broker TR

更新日期：1999-01-20 00:00:00

abstract：:The prototypic macrophage-tropic HIV-1 isolate, HIV-1BaL, cannot replicate in the monocytoid cell line THP-1. After induction of differentiation by a phorbol diester, a fraction of THP-1 cells became permissive to HIV-1BaL. In contrast, this treatment decreased permissiveness for the lymphotropic isolate HIV-1LAI. Vir...

journal_title：Virology

authors： Meylan PR,Spina CA,Richman DD,Kornbluth RS

更新日期：1993-03-01 00:00:00

abstract：:DNA obtained from New Zealand white rabbit oral papillomas was analyzed for the presence of papillomavirus DNA. The viral genome was cloned as three separate subclones, which were each mapped and oriented with respect to one another. Comparisons with other papillomavirus DNAs by Southern blot hybridization under vario...

journal_title：Virology

authors： O'Banion MK,Cialkowski ME,Reichmann ME,Sundberg JP

更新日期：1988-01-01 00:00:00

abstract：:Cauliflower mosaic virus (CaMV) uses a specialised translation mechanism to bypass the long leader sequence of the 35S RNA. The effect of the CaMV 35S RNA leader sequence on the expression of a downstream beta-glucuronidase (GUS) reporter gene was studied in transgenic tobacco plants. Enzymatic GUS assays of these tra...

journal_title：Virology

authors： Schärer-Hernández N,Hohn T

更新日期：1998-03-15 00:00:00

abstract：:Human parvovirus B19 (B19) infects human erythroid lineage cells. Accumulating evidence also shows that B19 is detectable in nonerythroid lineage cells in vivo, but the mechanism of infection is still not clear. In this study, we explored the mode of B19 infection of human monocytic cell line U937. An in vitro infecti...

journal_title：Virology

authors： Munakata Y,Kato I,Saito T,Kodera T,Ishii KK,Sasaki T

更新日期：2006-02-05 00:00:00

abstract：:RNA silencing is a fundamental antiviral response in eukaryotic organisms. We investigated the counterdefense strategy of a fungal virus (mycovirus) against RNA silencing in the white root rot fungus, Rosellinia necatrix. We generated an R. necatrix strain that constitutively induced RNA silencing of the exogenous gre...

journal_title：Virology

authors： Yaegashi H,Yoshikawa N,Ito T,Kanematsu S

更新日期：2013-09-01 00:00:00

abstract：:Very fast sedimenting DNA (VFS DNA) of T4 phage, which is formed by infection with a mutant in gene 49, was examined by electron microscopy after mild treatment with DNase I. It showed Y-shaped, branched strands in addition to linear strands. Each branch contained a single-stranded interruption about 60 nucleotides lo...

journal_title：Virology

authors： Minagawa T,Murakami A,Ryo Y,Yamagishi H

更新日期：1983-04-15 00:00:00

abstract：:The single open reading frame (ORF) 5 encoding the 50-kDa protein of barley yellow dwarf virus PAV-IL (BYDV-PAV-IL) was expressed in bacteria, purified, and used as an immunogen/antigen to produce/screen antibodies specific to the 50-kDa protein. Two monoclonal antibodies (MAb PAV-IL-22 kDa and MAb PAV-IL-50 kDa) rais...

journal_title：Virology

authors： Cheng SL,Domier LL,D'Arcy CJ

更新日期：1994-08-01 00:00:00

abstract：:Condylomata acuminata is caused by human papillomavirus (HPV). PCR with consensus primers will typically detect HPV in >96% of condylomata. Metagenomic sequencing has found that some "HPV-negative" condylomata do indeed contain HPV. We wished to perform a renewed evaluation of the "HPV-negative" condylomata using deep...

journal_title：Virology

authors： Arroyo Mühr LS,Bzhalava D,Lagheden C,Eklund C,Johansson H,Forslund O,Dillner J,Hultin E

更新日期：2015-11-01 00:00:00

abstract：:Human adenovirus type 5 (Ad5) has been the most popular platform for the development of oncolytic Ads. Alternative Ad serotypes with low seroprevalence might allow for improved anticancer efficacy in Ad5-immune patients. We studied the safety and efficacy of rare serotypes Ad6, Ad11 and Ad35. In vitro cytotoxicity of ...

journal_title：Virology

authors： Shashkova EV,May SM,Barry MA

更新日期：2009-11-25 00:00:00

abstract：:HPV16 E6 deregulates G1/S cell cycle progression through p53 degradation preventing transcription of the CDK inhibitor p21(WAF1). However, additional mechanisms independent of p53 inactivation appear to exist. Here, we report that HPV16 E6 targets the cellular factor p150(Sal2), which positively regulates p21(WAF1) tr...

journal_title：Virology

authors： Parroche P,Touka M,Mansour M,Bouvard V,Thépot A,Accardi R,Carreira C,Roblot GG,Sylla BS,Hasan U,Tommasino M

更新日期：2011-09-01 00:00:00

abstract：:Repression of human papillomavirus (HPV) E6 and E7 oncogenes in established cervical carcinoma cell lines causes senescence due to reactivation of cellular tumor suppressor pathways. Here, we determined whether ongoing expression of HPV16 or HPV18 oncogenes is required for the proliferation of primary human cervical c...

journal_title：Virology

authors： Magaldi TG,Almstead LL,Bellone S,Prevatt EG,Santin AD,DiMaio D

更新日期：2012-01-05 00:00:00

abstract：:From two human brain tumors SV40 genomes were isolated by recombinant DNA techniques. The SV40 genome cloned from a human meningioma DNA was shown to be indistinguishable from wild-type SV40. In contrast, the SV40 genome cloned from a human astrocytoma proved to be a nonviable deletion mutant with a truncated early re...

journal_title：Virology

authors： Krieg P,Scherer G

更新日期：1984-10-30 00:00:00

abstract：:We report the construction of herpes simplex virus 1 recombinants from which all or part of the coding sequences of four open reading frames have been deleted. In recombinants R7101 and R7108, the BamHI D' fragment containing the coding sequences of the dUTPase gene and the promoter-regulatory domain of a late gene wa...

journal_title：Virology

authors： Barker DE,Roizman B

更新日期：1990-08-01 00:00:00

abstract：:The polyomavirus hr-t class of mutants has served as a major prototype to study the function of middle T + small T in the virus lytic cycle, Biochem. Biophys. Acta 695 (2), 69-95). The properties of these middle T + small T defective mutants were defined by comparisons with "wild-type" strains reconstructed by marker ...

journal_title：Virology

authors： Spink KM,Fluck MM

更新日期：2003-03-15 00:00:00

abstract：:Prostaglandin E₂ (PGE₂) is an arachidonic acid (AA)-derived signaling molecule that can influence host immune responses to infection or vaccination. In this study, we investigated PGE₂ production in vitro by cells infected with the poxvirus vaccine strain, modified vaccinia Ankara virus (MVA). Human THP-1 cells, murin...

journal_title：Virology

authors： Pollara JJ,Spesock AH,Pickup DJ,Laster SM,Petty IT

更新日期：2012-07-05 00:00:00

abstract：:The complete genomes of 30 Soybean mosaic virus (SMV) isolates and strains were sequenced in this study. Together with fourteen previously reported sequences, we analyzed the genetic structure of the SMV population. Analyses of genetic diversity showed that different genomic regions of SMV are under different evolutio...

journal_title：Virology

authors： Seo JK,Ohshima K,Lee HG,Son M,Choi HS,Lee SH,Sohn SH,Kim KH

更新日期：2009-10-10 00:00:00

abstract：:Tandemers 2MG, 2MG3, 3MG and 4MG are derivatives of the potent anti-HIV-1 microbicide candidate griffithsin (GRFT). We compared these compounds anti-HIV-1 activity to GRFT using the viruses CAP206.08 and CAAN5342.A2 that have decreased sensitivity to this lectin. The 2MG and 2MG3 tandemers had similar activity to GRFT...

journal_title：Virology

authors： Alexandre K,Malatji K,Mulaudzi T

更新日期：2020-05-01 00:00:00