Production of prostaglandin E₂ in response to infection with modified vaccinia Ankara virus.

abstract：:The envelope glycoprotein (Env) of Moloney murine leukemia virus (MoMuLV) is proteolytically processed and transported to the cell surface where it can be incorporated into budding virions. Cell surface Env is frequently detected using an indirect immunofluorescence assay and fluorescence-activated cell sorting (FACS)...

journal_title：Virology

authors： Yu H,Empig C,Xia J,Anderson WF

更新日期：1998-04-10 00:00:00

abstract：:Human hepatitis B virus (HBV) envelopes contain three distinct glycoproteins called L, M, and S HBsAg. Each is posttranslationally modified to contain N-linked oligosaccharides. N-linked oligosaccharides, after attachment to a polypeptide backbone, are processed by enzymes within the endoplasmic reticulum (ER). There ...

journal_title：Virology

authors： Lu X,Mehta A,Dwek R,Butters T,Block T

更新日期：1995-11-10 00:00:00

abstract：:Hepatitis B virus synthesizes multiple spliced RNAs that can be reverse transcribed into viral DNA. We thoroughly characterized the contribution of spliced RNAs to DNA synthesis in transfected cultures of Huh7 and HepG2 cells. We found that up to 50% of DNA within intracellular capsids is derived from five spliced RNA...

journal_title：Virology

authors： Abraham TM,Lewellyn EB,Haines KM,Loeb DD

更新日期：2008-09-15 00:00:00

abstract：:A series of BglII- and Sau3A-derived fragments of phage T1 DNA were cloned into the plasmid vector pLV59 and the clones were tested for their ability to complement and recombine with infecting genomes of am mutants representing all known T1 essential genes. The resulting data were used to correlate the T1 genetic and ...

journal_title：Virology

authors： Liebeschuetz J,Ritchie DA

更新日期：1985-05-01 00:00:00

abstract：:DNA sequence and transcriptional analyses were performed on the region of the equine herpesvirus type 1 (EHV-1) genome (KyA strain) (map units 0.129 to 0.152) encoding open reading frames (ORFs) 15 and 16. ORF16 encodes a homolog of glycoprotein C of HSV-1 (herpes simplex virus type 1), while ORF15 corresponds in posi...

journal_title：Virology

authors： Matsumura T,Smith RH,O'Callaghan DJ

更新日期：1993-04-01 00:00:00

abstract：:Previous reports from our lab had shown that sera obtained from SIV(mac)-infected animals neutralized SIV(mac) infectivity in CD4(+) T cells but failed to protect monkey primary macrophages from infection with the virus. However, the antibodies could inhibit completion of the viral life cycle in the macrophages at the...

journal_title：Virology

authors： Zhuge W,Jia F,Mackay G,Kumar A,Narayan O

更新日期：2001-09-01 00:00:00

abstract：:Fixed rabies virus strains (ERA and CVS) produce a fatal paralytic disease in mice after intracerebral or intramuscular injection. Some antigenic mutants of both CVS and ERA viruses with a substitution in position 333 of the glycoprotein (arginine is replaced either by a glutamine, a glycine, or an isoleucine) are tot...

journal_title：Virology

authors： Tuffereau C,Leblois H,Bénéjean J,Coulon P,Lafay F,Flamand A

更新日期：1989-09-01 00:00:00

abstract：:An in-frame gag gene mutant of human immunodeficiency virus type 1, which carries two amino acid substitutions in the center of the p24 coding region, was constructed in vitro, and its replication properties in several cell lines were examined. In CD4-negative SW480 cells transfected with the mutant clone, synthesis a...

journal_title：Virology

authors： Sakuragi J,Sakai H,Kawamura M,Tokunaga K,Ueda S,Adachi A

更新日期：1995-09-10 00:00:00

abstract：:In an attempt to develop an in vitro human cytomegalovirus (HCMV) latency model system, the growth characteristics of HCMV in a human thyroid papillary carcinoma cell line (TPC-1) were examined. When TPC-1 cultures preheated at 40.5 degrees for 48 hr were infected with HCMV and incubated at a supraoptimal temperature ...

journal_title：Virology

authors： Tanaka J,Ogura T,Sato H,Hatano M

更新日期：1987-11-01 00:00:00

abstract：:A critical feature of highly pathogenic avian influenza viruses (H5N1 and H7N7) is the efficient intracellular cleavage of the hemagglutinin (HA) protein. H7N7 viruses also exist in equine species, and a unique feature of the equine H7N7 HA is the presence of an eleven amino acid insertion directly N-terminal to a tet...

journal_title：Virology

authors： Hamilton BS,Sun X,Chung C,Whittaker GR

更新日期：2012-12-05 00:00:00

abstract：:The function of rice dwarf virus segment 11 and the corresponding segments of other phytoreoviruses is not yet determined. The amino acid sequence of Pns11, encoded by segment 11, contains a putative zinc finger and five flanking basic regions at the C-terminus. The full-length Pns11 protein and three truncated deriva...

journal_title：Virology

authors： Xu H,Li Y,Mao Z,Li Y,Wu Z,Qu L,An C,Ming X,Schiemann J,Casper R,Chen Z

更新日期：1998-01-20 00:00:00

abstract：:Previous studies (Mertens et al., Virology 157, 375-386, 1987) have shown that removal of the outer capsid layer from bluetongue virus (BTV) significantly reduces (approximately x 10(-4)) the infectivity of the resultant core particle for mammalian cells (BHK 21 cells). In contrast, the studies reported here, using a ...

journal_title：Virology

authors： Mertens PP,Burroughs JN,Walton A,Wellby MP,Fu H,O'Hara RS,Brookes SM,Mellor PS

更新日期：1996-03-15 00:00:00

abstract：:Infection with the Tucson strain of coxsackievirus B1 (CVB1T) causes the development of chronic inflammatory myopathy (CIM) and hind limb weakness in susceptible strains of mice. In this study, a panel of six plaque-purified viruses exhibiting either small or large plaque phenotypes was derived from parental CVB1T and...

journal_title：Virology

authors： Tam PE,Messner RP

更新日期：1997-06-23 00:00:00

abstract：:GV1B is one of five viroids that have recently been purified from grapevines. GV1B has now been sequenced and its 363 nucleotide residues can potentially form the typical rod-like structure of viroids with 67% of nucleotides base-paired. GV1B has highest sequence similarity with grapevine yellow speckle viroid (GYSV; ...

journal_title：Virology

authors： Koltunow AM,Rezaian MA

更新日期：1989-06-01 00:00:00

abstract：:Transmissible gastroenteritis virus (TGEV) causes severe diarrhea and high mortality in newborn piglets. It is well established that porcine intestinal epithelium is the target of the TGEV infection, however the mechanism that TGEV invades the host epithelium remains largely unknown. Aminopeptidase N (APN) is a known ...

journal_title：Virology

authors： Hu W,Zhang S,Shen Y,Yang Q

更新日期：2018-08-01 00:00:00

abstract：:Ten human genital invasive squamous cell carcinomas and five human premalignant tissues were analyzed for the presence of selected sets of herpes simplex virus 2 (HSV-2) DNA sequences. Two vulvar tumors and one vulvar dysplastic tissue were found to contain DNA sequences homologous to the BglII O fragment (coordinates...

journal_title：Virology

authors： Manservigi R,Cassai E,Deiss LP,Di Luca D,Segala V,Frenkel N

更新日期：1986-11-01 00:00:00

abstract：:Hepatitis B viruses replicate via reverse transcription of an RNA intermediate. This RNA pregenome serves as mRNA and is packaged into capsids and reverse transcribed. Both processes require the interaction of the viral reverse transcriptase, P protein, with the 5'-proximal epsilon-signals on the pregenome. For epsilo...

journal_title：Virology

authors： Beck J,Bartos H,Nassal M

更新日期：1997-01-20 00:00:00

abstract：:The bovine papilloma virus (BPV) E1 protein essential to viral DNA replication has recently been shown to associate via direct protein-DNA interactions with the viral origin of replication and to be an ATP-dependent helicase. We show here that in accordance with the latter function, the E1 gene product has intrinsic A...

journal_title：Virology

authors： MacPherson P,Thorner L,Parker LM,Botchan M

更新日期：1994-10-01 00:00:00

abstract：:The polyomavirus hr-t class of mutants has served as a major prototype to study the function of middle T + small T in the virus lytic cycle, Biochem. Biophys. Acta 695 (2), 69-95). The properties of these middle T + small T defective mutants were defined by comparisons with "wild-type" strains reconstructed by marker ...

journal_title：Virology

authors： Spink KM,Fluck MM

更新日期：2003-03-15 00:00:00

abstract：:From two human brain tumors SV40 genomes were isolated by recombinant DNA techniques. The SV40 genome cloned from a human meningioma DNA was shown to be indistinguishable from wild-type SV40. In contrast, the SV40 genome cloned from a human astrocytoma proved to be a nonviable deletion mutant with a truncated early re...

journal_title：Virology

authors： Krieg P,Scherer G

更新日期：1984-10-30 00:00:00

abstract：:The herpes simplex virus type 1 (HSV-1) latency associated transcript (LAT) gene's anti-apoptosis activity plays a central, but not fully elucidated, role in enhancing the virus's reactivation phenotype. In transient transfection experiments, LAT increases cell survival following an apoptotic insult in the absence of ...

journal_title：Virology

authors： Carpenter D,Hsiang C,Brown DJ,Jin L,Osorio N,BenMohamed L,Jones C,Wechsler SL

更新日期：2007-12-05 00:00:00

abstract：:In vitro translation of pH 8-washed particles of tobacco mosaic virus (TMV; vulgare, common, or U1 strain) in a cell-free system derived from Escherichia coli (MRE 600) results in a broad size range of TMV RNA-encoded products, including significant amounts of the distinctive 126-kDa polypeptide which is encoded by th...

journal_title：Virology

authors： Wilson TM

更新日期：1986-07-15 00:00:00

abstract：:Foot-and-mouth disease virus (FMDV) VP1 G-H loop contains the major antigenic site. By replacing the sequence upstream of the RGD motif with a FLAG epitope, a marker virus for pathogenesis studies was generated. In cell culture, the recombinant virus containing FLAG (A24-FLAG) exhibited similar plaque phenotypes and g...

journal_title：Virology

authors： Lawrence P,Pacheco JM,Uddowla S,Hollister J,Kotecha A,Fry E,Rieder E

更新日期：2013-02-05 00:00:00

abstract：:ICP27 is an essential herpes simplex virus type 1 (HSV-1) nuclear protein which regulates viral early and late genes during infection. The exact mechanism by which ICP27 modulates viral gene expression is unknown, but considerable evidence suggests that it functions posttranscriptionally. In this study, we have asked ...

journal_title：Virology

authors： Mears WE,Rice SA

更新日期：1998-03-01 00:00:00

abstract：:A collection of randomly generated temperature-sensitive (ts) vaccinia virus (strain IHD-W) mutants were reported by S. Dales et al., (1978, Virology, 84, 403-428) in 1978 and characterized by electron microscopy. We have performed further genetic analysis on the Dales collection of mutants to make the mutants more us...

journal_title：Virology

authors： Lackner CA,D'Costa SM,Buck C,Condit RC

更新日期：2003-01-20 00:00:00

abstract：:Hepatitis delta virus (HDV) encodes only one protein, the hepatitis delta antigen (HDAg). Two forms of HDAg, a large (27 kDa) and a small (24 kDa) one, participate in the various steps of HDV replication. To further understand the properties of HDAg, we have constructed recombinant baculoviruses and expressed both for...

journal_title：Virology

authors： Hwang SB,Lee CZ,Lai MM

更新日期：1992-09-01 00:00:00

abstract：:Ten mutations were introduced into the JC virus (JCV) T antigen within a region corresponding to the SV40 T-antigen DNA binding domain (SV40 amino acids 131 to 220); nine of these increased homology between the two proteins in sequences critical for SV40 T antigen DNA binding. All mutant JCV T antigens bound to JCV an...

journal_title：Virology

authors： Tavis JE,Frisque RJ

更新日期：1991-07-01 00:00:00

abstract：:Measles virus V protein represses genome replication through a poorly understood mechanism, which led us to investigate whether V protein might be an RNA-binding modulatory factor. Recombinant V protein, expressed from transfected HEp-2 cells or E. coli, formed protein-RNA complexes with poly-guanosine (poly-G) or pol...

journal_title：Virology

authors： Parks CL,Witko SE,Kotash C,Lin SL,Sidhu MS,Udem SA

更新日期：2006-04-25 00:00:00

abstract：:The retroviral nucleocapsid (NC) protein contains highly conserved amino acid sequences (-Cys-X2-Cys-X4-His-X4-Cys-) designated retroviral (CCHC) Zn2+ fingers. The NC protein of murine leukemia viruses contains one NC Zn2+ finger and mutants that were competent in metal binding (CCCC and CCHH) packaged wild-type level...

journal_title：Virology

authors： Gorelick RJ,Gagliardi TD,Bosche WJ,Wiltrout TA,Coren LV,Chabot DJ,Lifson JD,Henderson LE,Arthur LO

更新日期：1999-03-30 00:00:00

abstract：:The function of a substantial percentage of the putative protein-coding open reading frames (ORFs) in viral genomes is unknown. As their sequence is not similar to that of proteins of known function, the function of these ORFs cannot be assigned on the basis of sequence similarity. Methods complement or in combination...

journal_title：Virology

authors： Han LY,Cai CZ,Ji ZL,Chen YZ

更新日期：2005-01-05 00:00:00