Abstract:
:The bovine papilloma virus (BPV) E1 protein essential to viral DNA replication has recently been shown to associate via direct protein-DNA interactions with the viral origin of replication and to be an ATP-dependent helicase. We show here that in accordance with the latter function, the E1 gene product has intrinsic ATPase activity. Mutations placed throughout the nucleotide binding consensus element abolish the ATPase activity of E1 and render BPV genomes harboring such mutations defective for episomal replication and impaired for oncogenic transformation.
journal_name
Virologyjournal_title
Virologyauthors
MacPherson P,Thorner L,Parker LM,Botchan Mdoi
10.1006/viro.1994.1544subject
Has Abstractpub_date
1994-10-01 00:00:00pages
403-8issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(84)71544-3journal_volume
204pub_type
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