Generation and characterization of a host cell-dependent gag gene mutant of human immunodeficiency virus type 1.

abstract：:Identification of regulators of p53 expression is a crucial step in understanding the diverse functions of p53 and its role in cellular homeostasis and responsiveness to insult. Several viral proteins inactivate p53 as a modulator of cell cycle progression and apoptosis. Here, we report that a unique leporipoxviral tr...

journal_title：Virology

authors： Wali A,Strayer DS

更新日期：1996-10-01 00:00:00

abstract：:The primer binding site (PBS) is involved in two stages during the reverse transcription of the retroviral RNA genome. In the early stage, the PBS provides complementary sequences through which tRNA(Lys,3) binds the viral RNA genome to initiate minus-strand DNA synthesis; in the later stages, complementarity between t...

journal_title：Virology

authors： Wakefield JK,Morrow CD

更新日期：1996-06-15 00:00:00

abstract：:Tissues from Australian brushtail possums (Trichosurus vulpecula) that had been experimentally infected with wobbly possum disease (WPD) virus (WPDV) were examined to elucidate pathogenesis of WPDV infection. Mononuclear inflammatory cell infiltrates were present in livers, kidneys, salivary glands and brains of WPD-a...

journal_title：Virology

authors： Giles J,Perrott M,Roe W,Shrestha K,Aberdein D,Morel P,Dunowska M

更新日期：2018-09-01 00:00:00

abstract：:Human infection with H7N9 virus has provoked global public health concern due to the substantial morbidity and mortality. Neuraminidase inhibitors (NAIs) are used as first-line drugs to treat the infection. However, virus quasispecies can evolve rapidly under drug pressure, which may alter various biological character...

journal_title：Virology

authors： Ge Y,Chi Y,Min X,Zhao K,Wu B,Wu T,Zhu X,Shi Z,Zhu F,Cui L

更新日期：2019-10-01 00:00:00

abstract：:The noncapsid protein expressed from ORF-206 of turnip yellow mosaic virus (TYMV) is autocatalytically processed by a papain-like protease, producing N-terminal 150-kDa and C-terminal 70-kDa proteins. By introducing two methionine residues near the N-terminus of the 70-kDa protein, we have obtained N-terminal amino ac...

journal_title：Virology

authors： Bransom KL,Wallace SE,Dreher TW

更新日期：1996-03-01 00:00:00

abstract：:Transfusion of blood and blood products contaminated with the pathogenic form of prion protein Prp(sc), thought to be the causative agent of variant a Creutzfeldt-Jakob disease (vCJD), may result in serious consequences in recipients with a compromised immune system, for example, as seen in HIV-1 infection. In the pre...

journal_title：Virology

authors： Bacot SM,Feldman GM,Yamada KM,Dhawan S

更新日期：2015-02-01 00:00:00

abstract：:We previously described a single-cycle dengue vaccine (RepliVAX D2) engineered from a capsid (C) gene-deleted West Nile virus (WNV) expressing dengue virus serotype 2 (DENV2) prM/E genes in place of the corresponding WNV genes. That work demonstrated that adaptation of RepliVAX D2 to grow in WNV C-expressing cells res...

journal_title：Virology

authors： Winkelmann ER,Widman DG,Suzuki R,Mason PW

更新日期：2011-12-20 00:00:00

abstract：:Epstein-Barr virus (EBV) DNA polymerase mediates viral DNA replication during the lytic phase of the EB virus life cycle. In order to characterize its enzymatic activities EBV DNA polymerase was purified more than 1200-fold from chemically induced B95-8 cells. One polypeptide with molecular weight of 110,000 correspon...

journal_title：Virology

authors： Tsurumi T

更新日期：1991-05-01 00:00:00

abstract：:It is now widely recognized that BPV-1 and less commonly BPV-2 are the causative agents of equine sarcoids. Here we present the generation of equine cell lines harboring BPV-1 genomes and expressing viral genes. These lines have been either explanted from sarcoid biopsies or generated in vitro by transfection of prima...

journal_title：Virology

authors： Yuan ZQ,Gault EA,Gobeil P,Nixon C,Campo MS,Nasir L

更新日期：2008-04-10 00:00:00

abstract：:Type III integral membrane proteins are oriented in the membrane with their C-terminus in the cytoplasm and their N-terminus extracytoplasmic. Such proteins are believed to have an internal hydrophobic sequence that functions both as an uncleaved signal for membrane insertion and also to anchor the protein in the memb...

journal_title：Virology

authors： Wilson-Rawls J,Deutscher SL,Wold WS

更新日期：1994-05-15 00:00:00

abstract：:Nineteen independent temperature-sensitive (ts) mutants were isolated from SA11 following mutagenesis with proflavin or 5-azacytidine. Fourteen of the ts mutants fell into one or another of five mutant groups previously defined by recombination. Five of the ts mutants defined five recombination groups (F, G, H, I, and...

journal_title：Virology

authors： Ramig RF

更新日期：1983-10-30 00:00:00

abstract：:Transgenic pigs are currently the most favored potential source of organs for xenotransplantation. Like all mammalian species they all harbor endogenous retroviruses in their genome. These porcine endogenous retroviruses (PERVs) are produced from several primary cells and cell lines and are able to infect human cells....

journal_title：Virology

authors： Tacke SJ,Kurth R,Denner J

更新日期：2000-03-01 00:00:00

abstract：:The extent of viral DNA methylation in non-virus-producing benign and malignant tumors induced by the Shope (rabbit) papilloma virus was investigated with the two isoschizomeric restriction endonucleases MspI and HpaII. The overall extent of methylation is variable in papillomas and uniformly higher in carcinomas. In ...

journal_title：Virology

authors： Wettstein FO,Stevens JG

更新日期：1983-04-30 00:00:00

abstract：:The M128L myxoma virus gene expresses a five-membrane spanning cell surface protein with significant amino acid homology to the cellular CD47 proteins. CD47, also called integrin-associated protein (IAP), is associated with the modulation of leukocyte adhesion, motility, activation, and phagocytosis. Creation of an M1...

journal_title：Virology

authors： Cameron CM,Barrett JW,Mann M,Lucas A,McFadden G

更新日期：2005-06-20 00:00:00

abstract：:Two cDNA clones, which hybridized in Northern blots to RNA segment 6 of the homologous avian-like group A rotavirus (RV) 993/83 and pigeon RV PO-13, but not to mammalian group A rotaviruses, were sequenced. The gene 6 sequence contained a single long open reading frame encoding a protein of 397 amino acids (total MW 4...

journal_title：Virology

authors： Rohwedder A,Irmak H,Werchau H,Brüssow H

更新日期：1993-08-01 00:00:00

abstract：:The bacteriophage T4 e gene encodes lysozyme (e-lysozyme), which releases progeny phage after normal infection of Escherichia coli cells. A mutation in the spackle gene suppresses the defect in e-lysozyme (Emrich, 1968). The spackle gene was mapped between genes 41 and 61, but its precise location has not previously b...

journal_title：Virology

authors： Kai T,Ueno H,Otsuka Y,Morimoto W,Yonesaki T

更新日期：1999-08-01 00:00:00

abstract：:We have constructed chimeric papillomavirus-like particles (CVLPs) by replacing the 34-carboxy-terminal amino acids of the HPV 16 L1 protein with various parts of the HPV 16 E7 protein. Chimeric proteins were expressed by recombinant baculoviruses and analyzed by electron microscopy for their ability to assemble into ...

journal_title：Virology

authors： Müller M,Zhou J,Reed TD,Rittmüller C,Burger A,Gabelsberger J,Braspenning J,Gissmann L

更新日期：1997-07-21 00:00:00

abstract：:We investigated the origin and the effect of insertion D67D-THGERDLGPA within HIV-1 RT from a patient failing antiviral therapy. The insertion developed within the context of pre-existing NRTI and NNRTI mutations (M41L, L210W, T215Y and N348I). Concurrently, the NRTI mutations T69I and V118I and the NNRTI mutations K1...

journal_title：Virology

authors： Megens S,Vaira D,De Baets G,Dekeersmaeker N,Schrooten Y,Li G,Schymkowitz J,Rousseau F,Vandamme AM,Moutschen M,Van Laethem K

更新日期：2014-05-01 00:00:00

abstract：:Recent reports indicated that vaccinia virus late mRNAs contain a unique 5' poly(A) leader sequence and that the in vitro translation of these mRNAs may be relatively cap-independent. These observations led us to examine the possibility that the mechanism of inhibition of host protein synthesis by vaccinia virus, like...

journal_title：Virology

authors： Schnierle BS,Moss B

更新日期：1992-06-01 00:00:00

abstract：:We describe studies of woodchuck hepatitis virus nucleic acids in liver and other tissues of chronically infected woodchucks, using Southern and Northern blot hybridization techniques. Single-stranded and covalently closed circular replicative DNA molecules were distinguished from partly double-stranded virus genomes....

journal_title：Virology

authors： Ogston CW,Schechter EM,Humes CA,Pranikoff MB

更新日期：1989-03-01 00:00:00

abstract：:The 24,000-molecular-weight major internal protein (p24) and the 15,000-molecular-weight nucleic acid binding protein (p15) of human T-cell leukemia virus type II (HTLV-II) were subjected to amino acid composition and amino-terminal amino acid sequence analysis. A comparison of amino acid composition of p24 and p15 of...

journal_title：Virology

authors： Devare SG,Kim Y,Fox JL,Getchell J,Cabradilla C,Kalyanaraman VS

更新日期：1985-04-15 00:00:00

abstract：:The orthopoxvirus serpin SPI-3 is N-glycosylated and suppresses fusion between infected cells. Although SPI-3 contains motifs conserved in inhibitory serpins, no proteinase inhibition by SPI-3 has been demonstrated, and mutations within the serpin reactive center loop (RCL) do not affect the ability to regulate cell f...

journal_title：Virology

authors： Turner PC,Baquero MT,Yuan S,Thoennes SR,Moyer RW

更新日期：2000-07-05 00:00:00

abstract：:LMP1 induces the expression of two members of the family of Id proteins, Id1 and Id3, and affects cell cycle regulation by decreasing the expression of the cyclin dependent kinase inhibitor, p27, and increasing levels and phosphorylation of cdk2 and Rb. In the present study, the contribution of the Id proteins to LMP1...

journal_title：Virology

authors： Everly DN Jr,Mainou BA,Raab-Traub N

更新日期：2008-07-05 00:00:00

abstract：:The P68 protein kinase (referred to as P68 based on its M(r) of 68,000 in human cells) is a serine/threonine kinase induced by interferon treatment and activated by dsRNAs. The kinase is under tight controls in virus-infected cells since once activated, it phosphorylates its natural substrate eukaryotic initiation fac...

journal_title：Virology

authors： Barber GN,Tomita J,Garfinkel MS,Meurs E,Hovanessian A,Katze MG

更新日期：1992-12-01 00:00:00

abstract：:The hypervariable region 1 (HVR1) of the putative envelope 2 protein of the hepatitis C virus (HCV) is the most variable part of the whole HCV polyprotein. Anti-HVR1 antibodies have been shown to protect against HCV infection, indicating that this region contains an important neutralization determinant. Recently we an...

journal_title：Virology

authors： Del Porto P,Puntoriero G,Scottà C,Nicosia A,Piccolella E

更新日期：2000-04-10 00:00:00

abstract：:The (guanine-N7)-methyltransferase domain of the vaccinia virus mRNA capping enzyme is a heterodimer composed of a catalytic subunit D1(498-844) bound to a stimulatory subunit D12. To identify structural elements of the 287-amino-acid D12 subunit that participate in binding and activation of the catalytic subunit, we ...

journal_title：Virology

authors： Saha N,Shuman S

更新日期：2001-08-15 00:00:00

abstract：:Chikungunya virus (CHIKV) is a mosquito-borne RNA virus that causes Chikungunya fever in humans. In this study, we generated two DNA-based CHIKV infectious clones derived from an Indian Ocean Lineage SL11131 strain and a prototype Ross strain. When the replication capabilities of the infectious CHIKV in various cell l...

journal_title：Virology

authors： Suzuki Y,Tanaka A,Maeda Y,Emi A,Fujioka Y,Sakaguchi S,Vasudevan SG,Kobayashi T,Lim CK,Takasaki T,Wu H,Nakano T

更新日期：2021-01-02 00:00:00

abstract：:Unlike cell lines transformed with Polyomaviruses, transformants derived by focus formation or colony formation in agarose medium after transfer into rat fibroblast cells (FR3T3 line) of Bovine Papilloma Type 1 (BPV1) DNA were consistently observed to grow poorly in suspension and to remain highly serum dependent for ...

journal_title：Virology

authors： Grisoni M,Meneguzzi G,de Lapeyrière O,Binétruy B,Rassoulzadegan M,Cuzin F

更新日期：1984-06-01 00:00:00

abstract：:Studies based on low-stringency hybridizations of radiolabeled swinepox virus (SPV) DNA to Southern blots containing DNA of representative members of the Orthopoxvirus, Leporipoxvirus, and Avipoxvirus genera and the Entomopoxvirus subfamily have revealed no DNA homology at this level of resolution. Antigenic relatedne...

journal_title：Virology

authors： Massung RF,Moyer RW

更新日期：1991-01-01 00:00:00

abstract：:The natural host of SIVrcm is the red-capped mangabey (Cercocebus torquatus torquatus). Although this virus infects macaques and human PBMCs, its pathogenic potential is unknown. We serially passaged SIVrcm through 9 rhesus macaques to assess its potential for virulence. SIVrcm infected all macaques with peak viremia ...

journal_title：Virology

authors： Ling B,Veazey RS,Marx PA

更新日期：2008-09-15 00:00:00