Abstract：
:We describe studies of woodchuck hepatitis virus nucleic acids in liver and other tissues of chronically infected woodchucks, using Southern and Northern blot hybridization techniques. Single-stranded and covalently closed circular replicative DNA molecules were distinguished from partly double-stranded virus genomes. In most animals the liver contained more virus than any other organ, but all extrahepatic organs studied (spleen, thymus, pancreas, and kidney) contained viral DNA and significant amounts of viral RNA. In the spleen, partly double-stranded virus genomes were present at higher levels than in any other organ but liver, but nonetheless replicative intermediates were not detected. The observation of RNA transcripts in the absence of detectable covalently closed circular DNA template suggests that a small proportion of cells in extrahepatic tissues are infected with WHV.
journal_name
Virologyjournal_title
Virologyauthors
Ogston CW,Schechter EM,Humes CA,Pranikoff MBdoi
10.1016/0042-6822(89)90035-4subject
Has Abstractpub_date
1989-03-01 00:00:00pages
9-14issue
1eissn
0042-6822issn
1096-0341journal_volume
169pub_type
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