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Recombinant measles viruses defective for RNA editing and V protein synthesis are viable in cultured cells.

Abstract：

:The measles virus (MV) phosphoprotein (P) gene encodes three proteins, P, C, and V. The V protein is synthesized by pseudo-templated transcription, also designated as RNA editing: during P gene transcription one G residue is inserted at a defined position in about 50% of the mRNAs. To study the importance of sequence elements for the nontemplated G insertion, we generated recombinant MVs in which six different mutations were introduced within the region where editing occurs (3' UUUUUCCC, template strand). These viruses were then analyzed for their ability to edit their P mRNA and to produce V protein. Single U to C changes within the U stretch abolished editing. Extending the template by three C residues at the site of G insertion resulted in a less precise editing phenotype and overproduction of V. None of these mutants were impaired in their multiplication behavior when analyzed in cultured cells. However, the syncytia of a recombinant MV overproducing V protein were in general smaller and lysed 1 to 2 days later than usual.

journal_name

Virology

journal_title

Virology

authors

Schneider H,Kaelin K,Billeter MA

doi

10.1006/viro.1996.8339

subject

Has Abstract

pub_date

1997-01-20 00:00:00

pages

314-22

issue

2

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(96)98339-7

journal_volume

227

pub_type

杂志文章

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