Abstract:
:The recent identification of a novel human coronavirus responsible of a SARS-like illness in the Middle-East a decade after the SARS pandemic, demonstrates that reemergence of a SARS-like coronavirus from an animal reservoir remains a credible threat. Because SARS is contracted by aerosolized contamination of the respiratory tract, a vaccine inducing mucosal long-term protection would be an asset to control new epidemics. To this aim, we generated live attenuated recombinant measles vaccine (MV) candidates expressing either the membrane-anchored SARS-CoV spike (S) protein or its secreted soluble ectodomain (Ssol). In mice susceptible to measles virus, recombinant MV expressing the anchored full-length S induced the highest titers of neutralizing antibodies and fully protected immunized animals from intranasal infectious challenge with SARS-CoV. As compared to immunization with adjuvanted recombinant Ssol protein, recombinant MV induced stronger and Th1-biased responses, a hallmark of live attenuated viruses and a highly desirable feature for an antiviral vaccine.
journal_name
Virologyjournal_title
Virologyauthors
Escriou N,Callendret B,Lorin V,Combredet C,Marianneau P,Février M,Tangy Fdoi
10.1016/j.virol.2014.01.002subject
Has Abstractpub_date
2014-03-01 00:00:00pages
32-41eissn
0042-6822issn
1096-0341pii
S0042-6822(14)00005-1journal_volume
452-453pub_type
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