Functional analysis of bipartite begomovirus coat protein promoter sequences.

Abstract:

:We demonstrate that the AL2 gene of Cabbage leaf curl virus (CaLCuV) activates the CP promoter in mesophyll and acts to derepress the promoter in vascular tissue, similar to that observed for Tomato golden mosaic virus (TGMV). Binding studies indicate that sequences mediating repression and activation of the TGMV and CaLCuV CP promoter specifically bind different nuclear factors common to Nicotiana benthamiana, spinach and tomato. However, chromatin immunoprecipitation demonstrates that TGMV AL2 can interact with both sequences independently. Binding of nuclear protein(s) from different crop species to viral sequences conserved in both bipartite and monopartite begomoviruses, including TGMV, CaLCuV, Pepper golden mosaic virus and Tomato yellow leaf curl virus suggests that bipartite begomoviruses bind common host factors to regulate the CP promoter. This is consistent with a model in which AL2 interacts with different components of the cellular transcription machinery that bind viral sequences important for repression and activation of begomovirus CP promoters.

journal_name

Virology

journal_title

Virology

authors

Lacatus G,Sunter G

doi

10.1016/j.virol.2008.03.012

subject

Has Abstract

pub_date

2008-06-20 00:00:00

pages

79-89

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(08)00183-9

journal_volume

376

pub_type

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