Abstract:
:Two novel halophilic archaebacterial bacteriophages, HF1 and HF2, were isolated from an Australian solar saltern. They were morphologically identical with icosahedral-shaped heads (diameter 58 nm) and contractile tails (length 94 nm). Other similarities included sensitivity to reduced ionic conditions, similar protein profiles by SDS-PAGE, and dsDNA genomes of identical size (73.5 kbp) with analogous restriction patterns. DNA-DNA hybridization data showed the two phages to be closely related. HF1 has a broad host-range, infecting members of three halobacterial genera including Halobacterium salinarium and the genetically well-characterized strain Haloferax volcanii WFD11. Mutants showing increased plating efficiency on alternative hosts were readily selectable. By contrast, HF2 showed a limited host range, confined to the closely related dam-methylated strains Ch2 and H. saccharovorum.
journal_name
Virologyjournal_title
Virologyauthors
Nuttall SD,Dyall-Smith MLdoi
10.1006/viro.1993.1643subject
Has Abstractpub_date
1993-12-01 00:00:00pages
678-84issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(83)71643-0journal_volume
197pub_type
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