Abstract:
:The bottom component B1a of TYMV with a buoyant density of 1.42-1.43 g/ml, is converted to a more dense particle B2a following CsCI density gradient centrifugation. This conversion is complete after a prior incubation of the virus in the CsCl solution at elevated temperatures. It is shown that the formation of B28 is accompanied with a loss from the virion of polyvalent cations like spermidine and Mg2+. The exchange of the latter ions for Cs ions is responsible for the occurrence of the higher-density particles. The more dense component B2a can be converted back to B,; like particles upon incubation in the presence of 0.1 M M9Cl2. If TYMV or component B1a is centrifuged in the presence of Mg2+, the conversion into B2a is prevented. The latter phenomenon allowed the isolation and characterization of the minor components B1b and B1c, previously postulated (Mellema et al. (1979), Virology 96, 38-46). The phenomenon of conversion appeared to be restricted to the components of the B series of TYMV; indicating that the class of light minor components does not contain artefacts of the CsCl gradient centrifugation. The formation of B2a is accompanied by degradation of RNA, which might be due to some conformational change in the virion, rendering the RNA vulnerable to RNases.
journal_name
Virologyjournal_title
Virologyauthors
Noort A,van den Dries CL,Pleij CW,Jaspars EM,Bosch Ldoi
10.1016/0042-6822(82)90041-1subject
Has Abstractpub_date
1982-07-30 00:00:00pages
412-21issue
2eissn
0042-6822issn
1096-0341pii
0042-6822(82)90041-1journal_volume
120pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::To identify the potential RNA inhibitors of HCV polymerase, we have isolated high-affinity RNA ligands specific to hepatitis C virus (HCV) NS5B protein from a combinatorial RNA library using the Systematic Evolution of Ligands by EXponential enrichment (SELEX) procedure. Thirty-seven selected ligands were classified i...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(02)00095-8
更新日期:2003-03-15 00:00:00
abstract::Bovine herpesvirus 1 (BHV-1) gene expression was examined by RNA blot hybridization using clones representing immediate-early, early, and late genes. An immediate-early protein gene probe hybridized with two transcripts, 3.4 and 5.8 kb, expressed by infected cells in the presence of cycloheximide (CH). During infectio...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90744-a
更新日期:1992-05-01 00:00:00
abstract::We have examined the synthesis of the T protein of the human polyomavirus, JCV, during productive infection in primary cultures of human fetal glial and kidney cells. Immunoprecipitation of protein extracts from virus infected cells revealed that the JCV large T protein from both the prototype Mad and HEK adapted stra...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90418-6
更新日期:1986-01-15 00:00:00
abstract::B-HIV1, an oligoclone of immortalized cells derived from human peripheral B lymphocytes infected in vitro with the TIIIB isolate of HIV-1, produces low levels of replication-competent HIV when propagated in 1% serum, but increases production > or = 5-fold after phorbol myristate acetate (PMA) exposure. Electron micros...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1499
更新日期:1993-10-01 00:00:00
abstract::The function of the 16-kDa protein encoded by tobacco rattle virus (TRV) RNA-1 was investigated by a mutational analysis of the 16-kDa protein gene. Transcripts of TRV RNA-1 produced from a full-length cDNA clone of TRV RNA-1 (SYM strain) remained infectious when the 16-kDa protein gene was disrupted by premature term...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90601-7
更新日期:1991-06-01 00:00:00
abstract::An infectious and full-length molecular clone of genomic human spumaretrovirus (HSRV) DNA was constructed. The infectivity of the pHSRV13 clone was demonstrated after transfection into susceptible cells by passage of HSRV-specific cytopathic effects as a cell-free culture supernatant, by electron microscopy of HSRV pa...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90820-2
更新日期:1991-09-01 00:00:00
abstract::Open reading frame 89 (ORF89) is one of the three genes that are believed to be involved in the latent infection of white spot syndrome virus (WSSV). Here, we report the structure and functional characterization of ORF89. cDNA sequencing, 5' RLM-RACE, and 3' RLM-RACE showed that ORF89 gene is transcribed into an unspl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.05.005
更新日期:2004-07-20 00:00:00
abstract::West Nile virus (WNV) has become firmly established in northeastern US, reemerging every summer since its introduction into North America in 1999. To determine whether WNV overwinters locally or is reseeded annually, we examined the patterns of viral lineage persistence and replacement in Connecticut over 10 consecuti...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.06.006
更新日期:2011-08-15 00:00:00
abstract::The prototype strain of Flanders virus (61-7484) has been adapted to grow in Vero cell cultures and a plaque assay has been developed. Electron micrographs of infected cells revealed rod-shaped virions budding in clusters from the plasma membrane, consistent with the tentative classification of Flanders virus as a mem...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90275-9
更新日期:1988-04-01 00:00:00
abstract::Replication of the nonsegmented, plus-stranded RNA genome of Cucumber necrosis tombusvirus (CNV) requires two essential overlapping viral-coded replication proteins, the p33 replication co-factor and the p92 RNA-dependent RNA polymerase. In this paper, we demonstrate that p33 is phosphorylated in vivo and in vitro by ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.08.006
更新日期:2005-12-05 00:00:00
abstract::Rotavirus infection was studied in adult nude mice (BALB/c background), alpha beta or gamma delta and alpha beta/gamma delta T cell receptor (TCR) knockout (-/-) mice (C57BL/6 and C57BL/6 x 129 backgrounds), and SCID mice (C57BL/6 background). The gamma delta TCR -/- mice cleared infection just like control mice. All ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8843
更新日期:1997-11-24 00:00:00
abstract::The hepatitis B virus core open reading frame with and without the precore domain was expressed in insect cells using a baculovirus expression system. Precore antigen was not properly processed in insect cells and was present in highly insoluble cytoplasmic aggregates. Core antigen without the precore domain formed co...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90247-o
更新日期:1990-05-01 00:00:00
abstract::G1/S transition of the cell cycle is blocked when cells are cultured without cell adhesion, even if nutrients and serum are present. Shift experiments involving transfer from adhesion to suspension cultures have revealed that cell adhesion in the early half of G1 phase is required for induction of DNA synthesis. We fo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0591
更新日期:1996-11-01 00:00:00
abstract::Although defensins are known to inhibit the replication of human influenza A virus (IAV) in vitro, their in vivo expression during IAV infection is not known. Here we investigated mRNA and protein expression of several beta-defensins in the airways of IAV infected mice. Expression of murine beta-defensin (MBD)-3 and -...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.07.024
更新日期:2008-10-10 00:00:00
abstract::Nucleotide sequence analysis has shown previously that the genomic-length mRNA (mRNA1) of the coronavirus infectious bronchitis virus (IBV) contains two large open reading frames (ORFs), 1a and 1b, with the potential to encode polyproteins of approximately 441 and 300 kDa, respectively. We have characterized the speci...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1128
更新日期:1995-04-01 00:00:00
abstract::The Rta (R transactivator) protein plays an essential role in the Epstein-Barr viral (EBV) lytic cascade. Rta activates viral gene expression by several mechanisms including direct and indirect binding to target viral promoters, synergy with EBV ZEBRA protein, and stimulation of cellular signaling pathways. We previou...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.01.022
更新日期:2009-04-10 00:00:00
abstract::The longest hepatitis B virus transcript is a 3.9-kb mRNA whose function remained unclear. In this study, we wished to identify the translation products and physiological role of this viral transcript. This transcript initiates from the X promoter region ignoring the inefficient and noncanonical viral polyadenylation ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00156-9
更新日期:2003-05-10 00:00:00
abstract::The large (L) proteins of non-segmented negative stranded (NNS) RNA viruses contain the core RNA dependent RNA polymerase activity for RNA replication and transcription as well as the activities for polyadenylating and capping the mRNA transcripts and for methylating the cap structures. There is currently no structura...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.08.041
更新日期:2008-12-05 00:00:00
abstract::Respiratory syncytial virus is a major cause of bronchiolitis in infants and pneumonia virus of mice (PVM) causes similar disease in mice. The impact of PVM infection in BALB/c and C57BL/6 neonates, and upon re-infection as elderly mice, was compared. As previously shown for adult mice, PVM caused more disease in BALB...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.07.019
更新日期:2015-11-01 00:00:00
abstract::The ability of HIV to match levels of viral mRNA to the activation state of the host cell may play a role in its ability to persist as well as to replicate. This linkage depends on the function of the viral transcriptional regulatory protein, Tat, which increases the efficiency of RNA elongation (transcriptional proce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9647
更新日期:1999-05-10 00:00:00
abstract::Hepatitis C virus (HCV) is efficient in the establishment of persistent infection. We have previously shown that HCV core protein inhibits T cell proliferation through its interaction with the complement receptor, gC1qR. Here we show that HCV core-induced inhibition of T cell proliferation involves a G(0)/G(1) cell cy...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00419-7
更新日期:2003-09-15 00:00:00
abstract::We used Cre/loxP recombination to swap targeting ligands present on the adenoviral capsid protein IX (pIX). A loxP-flanked sequence encoding poly-lysine (pK-binds heparan sulfate proteoglycans) was engineered onto the 3'-terminus of pIX, and the resulting fusion protein allowed for routine virus propagation. Growth of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.09.004
更新日期:2011-11-25 00:00:00
abstract::Two sets of primers derived from the 5'-terminal region and the NS5 region of the hepatitis G virus (HGV) genome were used to amplify PCR fragments from serum specimens obtained from different parts of the world. All PCR fragments from the 5'-terminal region (5'-PCR, n = 56) and from the NS5 region (NS5-PCR, n = 85) w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9592
更新日期:1999-03-15 00:00:00
abstract::Callitrichid hepatitis (CH) is a highly fatal, rodent-borne zoonosis of New World primates (family Callitrichidae) caused by lymphocytic choriomeningitis virus (LCMV). It is unclear whether virulence in Callitrichidae is associated with specific genetic or phylogenetic markers of the virus as only a partial S RNA sequ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0909
更新日期:2001-06-05 00:00:00
abstract::Cauliflower mosaic virus (CaMV) uses a specialised translation mechanism to bypass the long leader sequence of the 35S RNA. The effect of the CaMV 35S RNA leader sequence on the expression of a downstream beta-glucuronidase (GUS) reporter gene was studied in transgenic tobacco plants. Enzymatic GUS assays of these tra...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9038
更新日期:1998-03-15 00:00:00
abstract::Electron microscopy and sequence analysis have suggested the presence of distinct morphological regions within the reovirus cell attachment protein sigma 1. Kinking of purified sigma 1 observed by electron microscopy and sequence-predicted flexibility profiles suggest the presence of potential flexible regions in the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1465
更新日期:1994-08-15 00:00:00
abstract::The recent development of in vitro hepatitis C virus (HCV) RNA replication systems has provided useful tools for studying the intracellular anti-HCV activity of ribavirin. Ribavirin has been shown to: (1) induce "error catastrophe" in poliovirus, Proc. Natl. Acad. Sci. USA 98, 6895-6900), (2) be a pseudo-substrate of ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00152-1
更新日期:2003-06-05 00:00:00
abstract::Pandemic H1N1 influenza A (H1N1pdm) elicits stronger pulmonary inflammation than previously circulating seasonal H1N1 influenza A (sH1N1), yet mechanisms of inflammatory activation in respiratory epithelial cells during H1N1pdm infection are unclear. We investigated host responses to H1N1pdm/sH1N1 infection and virus ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.09.022
更新日期:2014-01-05 00:00:00
abstract::A chloroplast-localized papaya methionine sulfoxide reductase B1 (PaMsrB1) interacting with Papaya ringspot virus (PRSV) NIa-Pro was identified using a Sos recruitment two-hybrid system (SRS). SRS analysis of several deletion mutants of PRSV NIa-Pro and PaMsrB1 demonstrated that the C-terminal (residues 133-239) fragm...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.09.007
更新日期:2012-12-05 00:00:00
abstract::The expression of two small basic proteins (C and C') encoded by a second open reading frame of the New Jersey serotype of vesicular stomatitis virus (VSV) P gene was reported previously (Spiropoulou and Nichol, J. Virol., 67, 3103-3110, 1993). Here we found that the Indiana serotype virus also expressed C and C' prot...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0066
更新日期:1996-02-15 00:00:00