Mutation and replacement of the 16-kDa protein gene in RNA-1 of tobacco rattle virus.

abstract：:Synthesis of unintegrated retroviral DNA via reverse transcription is thought to involve two separate template switches. Minus-strand strong-stop DNA, a major product of in vitro reactions using detergent-treated virions, is synthesized prior to the first template switch. Plus-strand strong-stop DNA, which is found ba...

journal_title：Virology

authors： Li P,Stephenson AJ,Kuiper LJ,Burrell CJ

更新日期：1993-05-01 00:00:00

abstract：:Measles virus V protein represses genome replication through a poorly understood mechanism, which led us to investigate whether V protein might be an RNA-binding modulatory factor. Recombinant V protein, expressed from transfected HEp-2 cells or E. coli, formed protein-RNA complexes with poly-guanosine (poly-G) or pol...

journal_title：Virology

authors： Parks CL,Witko SE,Kotash C,Lin SL,Sidhu MS,Udem SA

更新日期：2006-04-25 00:00:00

abstract：:The HPIV2 V protein inhibits type I interferon (IFN) induction and signaling. To manipulate the V protein, whose coding sequence overlaps that of the polymerase-associated phosphoprotein (P), without altering the P protein, we generated an HPIV2 virus in which P and V are expressed from separate genes (rHPIV2-P+V). rH...

journal_title：Virology

authors： Schaap-Nutt A,D'Angelo C,Amaro-Carambot E,Nolan SM,Davis S,Wise SM,Higgins C,Bradley K,Kim O,Mayor R,Skiadopoulos MH,Collins PL,Murphy BR,Schmidt AC

更新日期：2010-10-10 00:00:00

abstract：:Activation of the human cytomegalovirus (HCMV) origin of replication (oriLyt) was previously demonstrated in transient transfection assays in permissive human fetal fibroblasts and nonpermissive Vero cells, and shown to require six viral proteins that function at the replication fork plus a number of HCMV products tha...

journal_title：Virology

authors： Reid GG,Ellsmore V,Stow ND

更新日期：2003-04-10 00:00:00

abstract：:We have examined FIV vif function in the context of molecular clone pF34 (GenBank Accession No. M25729). Rabbit antisera directed against the translation product of the vif gene identified a 29-kDa protein in tissue culture cells infected with FIV-pF34; this protein was not present in cultures of uninfected cells. Thu...

journal_title：Virology

authors： Shacklett BL,Luciw PA

更新日期：1994-11-01 00:00:00

abstract：:Hepatitis B viruses replicate via reverse transcription of an RNA intermediate. This RNA pregenome serves as mRNA and is packaged into capsids and reverse transcribed. Both processes require the interaction of the viral reverse transcriptase, P protein, with the 5'-proximal epsilon-signals on the pregenome. For epsilo...

journal_title：Virology

authors： Beck J,Bartos H,Nassal M

更新日期：1997-01-20 00:00:00

abstract：:By employing two different buffer solutions for polyacrylamide gel electrophoresis, all genome segments of Fiji disease and maize rough dwarf virus were separated. Fiji disease virus contains ten genome segments with approximate genome molecular weights of 19.26 x 10(6) and 19.85 x 10(6), depending on the buffer emplo...

journal_title：Virology

authors： Reddy DV,Boccardo G,Outridge R,Teakle DS,Black LM

更新日期：1975-01-01 00:00:00

abstract：:The recent identification of a novel human coronavirus responsible of a SARS-like illness in the Middle-East a decade after the SARS pandemic, demonstrates that reemergence of a SARS-like coronavirus from an animal reservoir remains a credible threat. Because SARS is contracted by aerosolized contamination of the resp...

journal_title：Virology

authors： Escriou N,Callendret B,Lorin V,Combredet C,Marianneau P,Février M,Tangy F

更新日期：2014-03-01 00:00:00

abstract：:NS35, a basic protein encoded by gene 8 of SA11 rotavirus, possesses RNA-binding activity and is essential for genome replication. To identify conserved regions in the NS35 gene and its protein product, we determined the nucleotide sequences of the NS35 gene for the mammalian and avian rotaviruses Wa, DS1, SA11 (Patto...

journal_title：Virology

authors： Patton JT,Salter-Cid L,Kalbach A,Mansell EA,Kattoura M

更新日期：1993-02-01 00:00:00

abstract：:The 5' end of the subgenomic RNA of rubella virus was determined by primer extension. The Maxam-Gilbert sequence ladder of the primer extension product contained a determinable sequence which was colinear with the complement of the sequence of the genomic RNA through nucleotide 3325 from the 3' end of the genomic RNA ...

journal_title：Virology

authors： Frey TK,Marr LD,Sanchez A,Simmons RB

更新日期：1989-01-01 00:00:00

abstract：:At least four hantavirus species cause disease with prominent renal involvement-hemorrhagic fever with renal syndrome (HFRS); and several hantavirus strains cause disease with significant pulmonary involvement-hantavirus pulmonary syndrome (HPS). The most prevalent and lethal hantaviruses associated with HFRS and HPS ...

journal_title：Virology

authors： Hooper JW,Custer DM,Smith J,Wahl-Jensen V

更新日期：2006-03-30 00:00:00

abstract：:Respiratory syncytial virus (RSV) induces the production of a number of cytokines and chemokines by activation of nuclear factor kappa B (NF-kappaB). The activation of NF-kappaB has been shown to depend on viral replication in the infected cells. In this study, we demonstrate that expression of RSV M2-1 protein, a tra...

journal_title：Virology

authors： Reimers K,Buchholz K,Werchau H

更新日期：2005-01-20 00:00:00

abstract：:Nucleotide sequence analysis of the genome of the baculovirus Bombyx mori nuclear polyhedrosis virus (BmNPV) identified 18 homologues of the Autographa californica NPV (AcNPV) lefs (late expression factor genes). These BmNPV lefs showed high (73-98%) amino acid sequence identities to AcNPV lefs and were localized to s...

journal_title：Virology

authors： Gomi S,Zhou CE,Yih W,Majima K,Maeda S

更新日期：1997-03-31 00:00:00

abstract：:Toscana (TOS) virus stocks strongly interfering with standard virus replication were obtained by sequential passages of virus in suckling mouse brain. Characterization of viral RNAs in these stocks showed the presence of a heterogeneous population of defective RNA molecules derived from the L genomic segment, in both ...

journal_title：Virology

authors： Marchi A,Nicoletti L,Accardi L,Di Bonito P,Giorgi C

更新日期：1998-06-20 00:00:00

abstract：:We have studied the assembly of human immunodeficiency virus (HIV-1) Gag-B-galactosidase (Gag-B-gal; GBG) fusion proteins into HIV particles in the presence of HIV Gag proteins. Release of fusion proteins from cells was measured by assay of media versus cellular B-gal activities and was dependent on co-expression of u...

journal_title：Virology

authors： Wang CT,Stegeman-Olsen J,Zhang Y,Barklis E

更新日期：1994-05-01 00:00:00

abstract：:HIV-1 envelope glycoproteins (Env) and Env-based immunogens usually do not interact efficiently with the inferred germline precursors of known broadly neutralizing antibodies (bNAbs). This deficiency may be one reason why Env and Env-based immunogens are not efficient at inducing bNAbs. We evaluated the binding of 15 ...

journal_title：Virology

authors： Sliepen K,Medina-Ramírez M,Yasmeen A,Moore JP,Klasse PJ,Sanders RW

更新日期：2015-12-01 00:00:00

abstract：:Plasmid p12929 was shown to integrate into the chromosome of Corynebacterium glutamicum RM3 and BL15. The minimal integrating fragment was subsequently defined. The arms flanking the integrated plasmid (attL and attR) were identified, allowing for the determination of the attP and the attB attachment sites. The attB s...

journal_title：Virology

authors： Moreau S,Le Marrec C,Blanco C,Trautwetter A

更新日期：1999-03-01 00:00:00

abstract：:The polycation DEAE-dextran increased the binding of VSV to BHK cells about fourfold over a wide range of VSV concentrations. The same proportion of bound virions was internalized by the cell in the presence or absence of DEAE-dextran. Viral primary RNA production was increased to the equivalent of a 4- to 4.5-fold in...

journal_title：Virology

authors： Bailey CA,Miller DK,Lenard J

更新日期：1984-02-01 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) glycoproteins K and B (gK and gB) are intimately involved in virus-induced fusion of cells. Certain mutations in the UL27 (gB) and UL53 (gK) genes confer a syncytial (syn) phenotype characterized by extensive fusion of infected cells and giving rise to multinucleated cells. We have ...

journal_title：Virology

authors： Hutchinson L,Graham FL,Cai W,Debroy C,Person S,Johnson DC

更新日期：1993-10-01 00:00:00

abstract：:Although v-src, the oncogene of Rous sarcoma virus, has been shown to be capable of inducing lethal tumors at visceral sites distal to the primary tumor mass, the mechanisms opposing visceral tumor formation remain to be elucidated. In the present study, we show that visceral tumors, many of which represent a metastas...

journal_title：Virology

authors： Halpern MS,Wisner TW,Brown IP,England JM

更新日期：1993-11-01 00:00:00

abstract：:The herpes simplex virus type 1 (HSV-1) latency associated transcript (LAT) gene's anti-apoptosis activity plays a central, but not fully elucidated, role in enhancing the virus's reactivation phenotype. In transient transfection experiments, LAT increases cell survival following an apoptotic insult in the absence of ...

journal_title：Virology

authors： Carpenter D,Hsiang C,Brown DJ,Jin L,Osorio N,BenMohamed L,Jones C,Wechsler SL

更新日期：2007-12-05 00:00:00

abstract：:Marburg virus (MBGV), for which no vaccines or treatments currently exist, causes an acute hemorrhagic fever with a high mortality rate in humans. We previously showed that immunization with either killed MBGV or a glycoprotein (GP) subunit prevented lethal infection in guinea pigs. In the studies reported here, an RN...

journal_title：Virology

authors： Hevey M,Negley D,Pushko P,Smith J,Schmaljohn A

更新日期：1998-11-10 00:00:00

abstract：:The APOBEC3 enzyme family are host restriction factors that induce mutagenesis of HIV-1 proviral genomes through the deamination of cytosine to form uracil in nascent single-stranded (-)DNA. HIV-1 suppresses APOBEC3 activity through the HIV-1 protein Vif that induces APOBEC3 degradation. Here we compared two common po...

journal_title：Virology

authors： Mohammadzadeh N,Follack TB,Love RP,Stewart K,Sanche S,Chelico L

更新日期：2019-01-15 00:00:00

abstract：:While DNA vaccines have been shown to prime cellular immune responses, levels are often low in nonhuman primates or humans. Hence, efforts have been directed toward boosting responses by combining DNA with different vaccination modalities. To this end, a polyvalent DNA prime/protein boost vaccine, consisting of codon ...

journal_title：Virology

authors： Cristillo AD,Wang S,Caskey MS,Unangst T,Hocker L,He L,Hudacik L,Whitney S,Keen T,Chou TH,Shen S,Joshi S,Kalyanaraman VS,Nair B,Markham P,Lu S,Pal R

更新日期：2006-03-01 00:00:00

abstract：:In 2006, an exotic reassortant orbivirus, epizootic hemorrhagic disease virus serotype 6 (EHDV-6) [strain (Indiana)], was first detected in the United States. To characterize the reassortment configuration of this virus and to conclusively determine the parental virus of each RNA segment, the complete genome of EHDV-6...

journal_title：Virology

authors： Allison AB,Holmes EC,Potgieter AC,Wright IM,Sailleau C,Breard E,Ruder MG,Stallknecht DE

更新日期：2012-03-01 00:00:00

abstract：:High-risk human papillomaviruses (HPVs) are etiologically implicated in human cervical cancer. Two viral genes, E6 and E7, are commonly found expressed in these cancer cells. We have previously shown that mice transgenic for the HPV-16 E6 gene or E7 gene, in which the E6 or E7 was expressed in the basal layer of epith...

journal_title：Virology

authors： Song S,Liem A,Miller JA,Lambert PF

更新日期：2000-02-15 00:00:00

abstract：:The M128L myxoma virus gene expresses a five-membrane spanning cell surface protein with significant amino acid homology to the cellular CD47 proteins. CD47, also called integrin-associated protein (IAP), is associated with the modulation of leukocyte adhesion, motility, activation, and phagocytosis. Creation of an M1...

journal_title：Virology

authors： Cameron CM,Barrett JW,Mann M,Lucas A,McFadden G

更新日期：2005-06-20 00:00:00

abstract：:Infectious bronchitis virus (IBV) produces six subgenomic (sg) mRNAs, each containing a 64 nucleotide (nt) leader sequence, derived from the 5' end of the genome by a discontinuous process. Several putative functional domains such as a papain-like proteinase (PL(pro)), main protease (M(pro)), RNA-dependent RNA polymer...

journal_title：Virology

authors： Mondal SP,Cardona CJ

更新日期：2004-06-20 00:00:00

abstract：:The interaction between the viral envelope protein gp120 and the cellular surface antigen CD4 is a key event in HIV-1 infection. Reciprocal high affinity binding sites have been located in the first domain of CD4 and in the carboxy-terminal region of gp120, respectively. Upon infection, the membranes of the target cel...

journal_title：Virology

authors： Autiero M,Abrescia P,Dettin M,Di Bello C,Guardiola J

更新日期：1991-12-01 00:00:00

abstract：:The mutations in two DNA-negative ts mutants of herpes simplex virus type 1 (HSV-1), tsK13 and tsM19, have been previously mapped to a 2.0-kb fragment (coordinates 0.095-0.108) at the left end of the genome (S. Weller, D. Aschman, W. Sacks, D. Coen, and P. Schaffer, 1983, Virology 130, 290-305). Sequence analysis of t...

journal_title：Virology

authors： Zhu L,Weller SK

更新日期：1988-10-01 00:00:00