Characterization of Toscana virus-defective interfering particles generated in vivo.


:Toscana (TOS) virus stocks strongly interfering with standard virus replication were obtained by sequential passages of virus in suckling mouse brain. Characterization of viral RNAs in these stocks showed the presence of a heterogeneous population of defective RNA molecules derived from the L genomic segment, in both nucleocapsid (NC) and messenger RNAs, suggesting that these molecules could be replicated, assembled, and transcribed. Subgenomic RNAs from the L segment but not from the S or M segments were found in cells infected with these stocks. Defective RNA molecules interfered with virus replication and retained 5' and 3' genomic termini. Nucleotide sequence analysis of some cloned defective interfering (DI) RNAs revealed they contained one or more internal deletions reducing their length to 7-13% of the full-length L segment. An identical sequence motif, of variable length, was found at both terminal sites of the RNA junction on standard L sequences. This motif was retained only in one copy in the subgenomic RNA. These results are consistent with the generation of TOS virus DI particles in vivo and suggest that the defective genomic RNAs could be generated by polymerase jumping from a sequence to an identical one spatially closed because of the RNA structure.






Marchi A,Nicoletti L,Accardi L,Di Bonito P,Giorgi C




Has Abstract


1998-06-20 00:00:00
















  • No role for pepstatin-A-sensitive acidic proteinases in reovirus infections of L or MDCK cells.

    abstract::Strong evidence indicates that virions of mammalian reoviruses undergo proteolytic processing by acid-dependent cellular proteinases as an essential step in productive infection. Proteolytic processing takes the form of a series of cleavages of outer-capsid proteins final sigma3 and mu1/mu1C. Previous studies showed a...


    pub_type: 杂志文章


    authors: Kothandaraman S,Hebert MC,Raines RT,Nibert ML

    更新日期:1998-11-25 00:00:00

  • Interaction of rotavirus cores with the nonstructural glycoprotein NS28.

    abstract::The nonstructural rotavirus receptor glycoprotein NS28 is 175 amino acids long and oriented in the RER membrane with the NH2 terminus on the luminal side and approximately 131 amino acids accessible from the cytoplasmic side. Au et al. (1988) have demonstrated that NS28 is able to interact with rotavirus single-shelle...


    pub_type: 杂志文章


    authors: Meyer JC,Bergmann CC,Bellamy AR

    更新日期:1989-07-01 00:00:00

  • The virion N protein of infectious bronchitis virus is more phosphorylated than the N protein from infected cell lysates.

    abstract::Because phosphorylation of the infectious bronchitis virus (IBV) nucleocapsid protein (N) may regulate its multiple roles in viral replication, the dynamics of N phosphorylation were examined. 32P-orthophosphate labeling and Western blot analyses confirmed that N was the only viral protein that was phosphorylated. Pul...


    pub_type: 杂志文章


    authors: Jayaram J,Youn S,Collisson EW

    更新日期:2005-08-15 00:00:00

  • Genetic analysis of bipartite geminivirus tissue tropism.

    abstract::The bipartite geminiviruses bean golden mosaic virus (BGMV), cabbage leaf curl virus (CabLCV), and tomato golden mosaic virus (TGMV) exhibit differential tissue tropism in Nicotiana benthamiana. In systemically infected leaves, BGMV remains largely confined to vascular-associated cells (phloem-limited), whereas CabLCV...


    pub_type: 杂志文章


    authors: Qin Y,Petty IT

    更新日期:2001-12-20 00:00:00

  • Destabilization of the RB tumor suppressor protein and stabilization of p53 contribute to HPV type 16 E7-induced apoptosis.

    abstract::Cells that express the human papillomavirus (HPV) type 16 E7 oncoprotein are predisposed to undergo apoptosis. Transgenic mice that have E7 expression targeted to either the retinal photoreceptor cells or the lens cells exhibit signs of apoptosis in cells attempting to undergo differentiation. We established a cell cu...


    pub_type: 杂志文章


    authors: Jones DL,Thompson DA,Münger K

    更新日期:1997-12-08 00:00:00

  • Primary HIV-1 R5 isolates from end-stage disease display enhanced viral fitness in parallel with increased gp120 net charge.

    abstract::To better understand the evolution of the viral envelope glycoproteins (Env) in HIV-1 infected individuals who progress to AIDS maintaining an exclusive CCR5-using (R5) virus population, we cloned and sequenced the env gene of longitudinally obtained primary isolates. A shift in the electrostatic potential towards an ...


    pub_type: 杂志文章


    authors: Repits J,Sterjovski J,Badia-Martinez D,Mild M,Gray L,Churchill MJ,Purcell DF,Karlsson A,Albert J,Fenyö EM,Achour A,Gorry PR,Jansson M

    更新日期:2008-09-15 00:00:00

  • Antiviral activity of lycorine against Zika virus in vivo and in vitro.

    abstract::The emergence and re-emergence of Zika virus (ZIKV), is a cause for international concern. These highly pathogenic arboviruses represent a serious health burden in tropical and subtropical areas worldwide. Despite these burdens, antiviral therapies do not exist, and inhibitors of ZIKV are therefore urgently needed. To...


    pub_type: 杂志文章


    authors: Chen H,Lao Z,Xu J,Li Z,Long H,Li D,Lin L,Liu X,Yu L,Liu W,Li G,Wu J

    更新日期:2020-07-01 00:00:00

  • Identification and characterization of an equine herpesvirus 1 late gene encoding a potential zinc finger.

    abstract::In this report, we present the DNA sequence and transcriptional characterization of a gene (IR5) that maps within each of the inverted repeat (IR) segments of the equine herpesvirus type 1 (EHV-1) genome. The IR5 open reading frame (ORF) is located within both IR sequences (nucleotides 9932-10,642 of the IR). DNA sequ...


    pub_type: 杂志文章


    authors: Holden VR,Yalamanchili RR,Harty RN,O'Callaghan DJ

    更新日期:1992-06-01 00:00:00

  • Herpes simplex virus (HSV) glycoproteins B and K inhibit cell fusion induced by HSV syncytial mutants.

    abstract::Herpes simplex virus type 1 (HSV-1) glycoproteins K and B (gK and gB) are intimately involved in virus-induced fusion of cells. Certain mutations in the UL27 (gB) and UL53 (gK) genes confer a syncytial (syn) phenotype characterized by extensive fusion of infected cells and giving rise to multinucleated cells. We have ...


    pub_type: 杂志文章


    authors: Hutchinson L,Graham FL,Cai W,Debroy C,Person S,Johnson DC

    更新日期:1993-10-01 00:00:00

  • MHV-A59 enters polarized murine epithelial cells through the apical surface but is released basolaterally.

    abstract::Coronaviruses have a marked tropism for epithelial cells. Entry and release of the porcine transmissible gastroenteritis virus (TGEV) is restricted to apical surfaces of polarized epithelial cells, as we have recently shown (J. W. A. Rossen, C. P. J. Bekker, W. F. Voorhout, G. J. A. M. Strous, A. van der Ende, and P. ...


    pub_type: 杂志文章


    authors: Rossen JW,Voorhout WF,Horzinek MC,van der Ende A,Strous GJ,Rottier PJ

    更新日期:1995-06-20 00:00:00

  • Contribution of the gp120 V3 loop to envelope glycoprotein trimer stability in primate immunodeficiency viruses.

    abstract::The V3 loop of the human immunodeficiency virus type 1 (HIV-1) gp120 exterior envelope glycoprotein (Env) becomes exposed after CD4 binding and contacts the coreceptor to mediate viral entry. Prior to CD4 engagement, a hydrophobic patch located at the tip of the V3 loop stabilizes the non-covalent association of gp120...


    pub_type: 杂志文章


    authors: Bowder D,Hollingsead H,Durst K,Hu D,Wei W,Wiggins J,Medjahed H,Finzi A,Sodroski J,Xiang SH

    更新日期:2018-08-01 00:00:00

  • Raji B cells, misidentified as THP-1 cells, stimulate DC-SIGN-mediated HIV transmission.

    abstract::A number of studies examining interactions of dendritic cell (DC)-specific ICAM-3 grabbing nonintegrin (DC-SIGN) with viral pathogens have relied on monocytic transfectants as models for primary DCs. Here we show that the presumed "THP-1" monocytic cells used in these studies are instead Raji B cells. Moreover, we dem...


    pub_type: 杂志文章


    authors: Wu L,Martin TD,Carrington M,KewalRamani VN

    更新日期:2004-01-05 00:00:00

  • A variant of CRPV DNA preferentially maintained as a plasmid in NIH 3T3 cells and characterization of its transcripts in nude mouse tumors.

    abstract::Rabbit and human papillomaviruses are strictly epitheliotropic and their DNA replicates extrachromosomally in benign lesions (warts) of their natural host. Their tissue and host specificity is thought to be genetically controlled and may account for the inefficiency in transforming heterologous cells. In our hands, CR...


    pub_type: 杂志文章


    authors: Nasseri M,Wettstein FO

    更新日期:1987-12-01 00:00:00

  • Both homo and heterodimers of Marek's disease virus encoded Meq protein contribute to transformation of lymphocytes in chickens.

    abstract::Marek' disease virus serotype-1, also know as Gallid herpesvirus 2 (GaHV-2), elicits T-cell lymphomas in chickens. The GaHV-2 genome encodes an oncoprotein, Meq, with similarity to the Jun/Fos family of proteins. We have previously shown that Meq homodimers are not sufficient to induce lymphomas in chickens. In this s...


    pub_type: 杂志文章


    authors: Suchodolski PF,Izumiya Y,Lupiani B,Ajithdoss DK,Lee LF,Kung HJ,Reddy SM

    更新日期:2010-04-10 00:00:00

  • Induction of a protective antibody response to foot and mouth disease virus in mice following oral or parenteral immunization with alfalfa transgenic plants expressing the viral structural protein VP1.

    abstract::The utilization of transgenic plants expressing recombinant antigens to be used in the formulation of experimental immunogens has been recently communicated. We report here the development of transgenic plants of alfalfa expressing the structural protein VP1 of foot and mouth disease virus (FMDV). The presence of the ...


    pub_type: 杂志文章


    authors: Wigdorovitz A,Carrillo C,Dus Santos MJ,Trono K,Peralta A,Gómez MC,Ríos RD,Franzone PM,Sadir AM,Escribano JM,Borca MV

    更新日期:1999-03-15 00:00:00

  • Genetic characterization of an adapted pandemic 2009 H1N1 influenza virus that reveals improved replication rates in human lung epithelial cells.

    abstract::The 2009 influenza pandemic originated from a swine-origin H1N1 virus, which, although less pathogenic than anticipated, may acquire additional virulence-associated mutations in the future. To estimate the potential risk, we sequentially passaged the isolate A/Hamburg/04/2009 in A549 human lung epithelial cells. After...


    pub_type: 杂志文章


    authors: Wörmann X,Lesch M,Welke RW,Okonechnikov K,Abdurishid M,Sieben C,Geissner A,Brinkmann V,Kastner M,Karner A,Zhu R,Hinterdorfer P,Anish C,Seeberger PH,Herrmann A,Meyer TF,Karlas A

    更新日期:2016-05-01 00:00:00

  • Mutations in conserved domain I of the Sendai virus L polymerase protein uncouple transcription and replication.

    abstract::To begin to map functional domains of the Sendai P-L RNA polymerase complex we wanted to characterize the P binding site on the Sendai L protein. Analysis of in vitro and in vivo P-L polymerase complex formation with carboxyl-truncations of the L protein showed that the N-terminal half of the protein was required. Sit...


    pub_type: 杂志文章


    authors: Chandrika R,Horikami SM,Smallwood S,Moyer SA

    更新日期:1995-11-10 00:00:00

  • Inducible system in human hepatoma cell lines for hepatitis C virus production.

    abstract::We cloned the complete complementary DNA of an isolate of the hepatitis C virus, HCV-S1, into a tetracycline-inducible expression vector and stably transfected it into two human hepatoma cell lines, Huh7 and HepG2. Twenty-six Huh7 and two HepG2-positive clones were obtained after preliminary screening. Two Huh7 (SH-7 ...


    pub_type: 杂志文章


    authors: Lim SP,Soo HM,Tan YH,Brenner S,Horstmann H,MacKenzie JM,Ng ML,Lim SG,Hong Wj

    更新日期:2002-11-10 00:00:00

  • Epitopes on foot-and-mouth disease virus particles. I. Topology.

    abstract::Monoclonal antibodies (MAb) against an O1 Suisse isolate of FMDV were used to identify epitopes on the virus particle and to determine their relative function. Six major antigenic sites containing one or more epitopes were identified using competition ELISA. An epitope relationship is proposed consisting of a trypsin-...


    pub_type: 杂志文章


    authors: McCullough KC,Crowther JR,Carpenter WC,Brocchi E,Capucci L,De Simone F,Xie Q,McCahon D

    更新日期:1987-04-01 00:00:00

  • The flavonoid apigenin inhibits hepatitis C virus replication by decreasing mature microRNA122 levels.

    abstract::Despite recent progress in the development of direct-acting antivirals against hepatitis C virus (HCV), chronic HCV infection remains an important health burden worldwide. MicroRNA122 (miR122), a liver-specific microRNA (miRNA), positively regulates HCV replication, and systemic application of antisense oligonucleotid...


    pub_type: 杂志文章


    authors: Shibata C,Ohno M,Otsuka M,Kishikawa T,Goto K,Muroyama R,Kato N,Yoshikawa T,Takata A,Koike K

    更新日期:2014-08-01 00:00:00

  • Identification and characterization of a new base substitution in the vaccine strain of Sabin 3 poliovirus.

    abstract::The complete RNA sequence of Sabin 3 (LED3) used in vaccine in the United States has been determined. The LED3 Sabin 3 sequence contains the attenuating mutations at bases 472 and 2034 but differs from that published by Stanway et al. (Nucleic Acids Res., 11, 5629-5643, 1983) at two other base positions, 2493 and 6061...


    pub_type: 杂志文章


    authors: Weeks-Levy C,Tatem JM,DiMichele SJ,Waterfield W,Georgiu AF,Mento SJ

    更新日期:1991-12-01 00:00:00

  • Intranasal IgG4/7 antibody responses protect horses against equid herpesvirus-1 (EHV-1) infection including nasal virus shedding and cell-associated viremia.

    abstract::Equid herpesvirus-1 (EHV-1) outbreaks continue despite widely used vaccination. We demonstrated previously that an ORF1/ORF71 gene deletion mutant of the EHV-1 strain Ab4 (Ab4ΔORF1/71) is less virulent than its parent Ab4 virus. Here, we describe the Ab4 challenge infection evaluating protection induced by the Ab4ΔORF...


    pub_type: 杂志文章


    authors: Perkins G,Babasyan S,Stout AE,Freer H,Rollins A,Wimer CL,Wagner B

    更新日期:2019-05-01 00:00:00

  • Perturbation of differentiated functions during viral infection in vivo. II. Viral reassortants map growth hormone defect to the S RNA of the lymphocytic choriomeningitis virus genome.

    abstract::Lymphocytic choriomeningitis virus (LCMV) Armstrong caused disordered growth and glucose metabolism secondary to growth hormone deficiency in infected C3H/St mice. In contrast, LCMV strain WE replicated in fewer growth hormone-producing cells, failed to disrupt growth hormone synthesis, and did not cause growth hormon...


    pub_type: 杂志文章


    authors: Riviere Y,Ahmed R,Southern P,Oldstone MB

    更新日期:1985-04-15 00:00:00

  • Strain variation in Epstein-Barr virus immediate early genes.

    abstract::RNase protection analysis of mRNAs for Epstein-Barr virus (EBV) genes activated early in the induction of the productive cycle in Akata cells has confirmed that BZLF1 and BRLF1 behave as immediate early genes. These are likely to be the only virus immediate early genes since analysis of three other transcripts previou...


    pub_type: 杂志文章


    authors: Packham G,Brimmell M,Cook D,Sinclair AJ,Farrell PJ

    更新日期:1993-02-01 00:00:00

  • The ubiquitin-proteasome system is essential for the productive entry of Japanese encephalitis virus.

    abstract::The host-virus interaction during the cellular entry of Japanese encephalitis virus (JEV) is poorly characterized. The ubiquitin-proteasome system (UPS), the major intracellular proteolytic pathway, mediates diverse cellular processes, including endocytosis and signal transduction, which may be involved in the entry o...


    pub_type: 杂志文章


    authors: Wang S,Liu H,Zu X,Liu Y,Chen L,Zhu X,Zhang L,Zhou Z,Xiao G,Wang W

    更新日期:2016-11-01 00:00:00

  • ZikaBase: An integrated ZIKV- Human Interactome Map database.

    abstract::Re-emergence of ZIKV has caused infections in more than 1.5 million people. The molecular mechanism and pathogenesis of ZIKV is not well explored due to unavailability of adequate model and lack of publically accessible resources to provide information of ZIKV-Human protein interactome map till today. This study made ...


    pub_type: 杂志文章


    authors: Gurumayum S,Brahma R,Naorem LD,Muthaiyan M,Gopal J,Venkatesan A

    更新日期:2018-01-15 00:00:00

  • Mutations within A 35 amino acid region of P6 influence self-association, inclusion body formation, and Caulimovirus infectivity.

    abstract::Cauliflower mosaic virus gene VI product (P6) is an essential protein that forms cytoplasmic, inclusion bodies (IBs). P6 contains four regions involved in self-association, termed D1-D4. D3 binds to D1, along with D4 and contains a spacer region (termed D3b) between two RNA-binding domains. Here we show D3b binds full...


    pub_type: 杂志文章


    authors: Lutz L,Okenka G,Schoelz J,Leisner S

    更新日期:2015-02-01 00:00:00

  • The genome of camelpox virus.

    abstract::Camelpox virus (CMLV), a member of the Orthopoxvirus genus in the Poxviridae, is the etiologic agent of a disease of camels. Here we report the CMLV genomic sequence with analysis. The 205,719-bp CMLV genome contains 211 putative genes and consists of a central region bound by identical inverted terminal repeats of ap...


    pub_type: 杂志文章


    authors: Afonso CL,Tulman ER,Lu Z,Zsak L,Sandybaev NT,Kerembekova UZ,Zaitsev VL,Kutish GF,Rock DL

    更新日期:2002-03-30 00:00:00

  • Rapid in vivo induction of HIV-specific CD8+ cytotoxic T lymphocytes by a 15-amino acid unmodified free peptide from the immunodominant V3-loop of GP120.

    abstract::Efforts to generate a vaccine to prevent infection by human immunodeficiency virus (HIV) have focused on inducing neutralizing antibodies. However, cytotoxic T-lymphocyte (CTL) responses are a major immune defense mechanism required for recovery from many different virus infections. Since CTL epitopes can be defined b...


    pub_type: 杂志文章


    authors: Sastry KJ,Nehete PN,Venkatnarayanan S,Morkowski J,Platsoucas CD,Arlinghaus RB

    更新日期:1992-06-01 00:00:00

  • Large-scale analysis of influenza A virus nucleoprotein sequence conservation reveals potential drug-target sites.

    abstract::The nucleoprotein (NP) of the influenza A virus encapsidates the viral RNA and participates in the infectious life cycle of the virus. The aims of this study were to find the degree of conservation of NP among all virus subtypes and hosts and to identify conserved binding sites, which may be utilised as potential drug...


    pub_type: 杂志文章


    authors: Kukol A,Hughes DJ

    更新日期:2014-04-01 00:00:00