Binding of inferred germline precursors of broadly neutralizing HIV-1 antibodies to native-like envelope trimers.

abstract：:Cauliflower mosaic virus gene VI product (P6) is an essential protein that forms cytoplasmic, inclusion bodies (IBs). P6 contains four regions involved in self-association, termed D1-D4. D3 binds to D1, along with D4 and contains a spacer region (termed D3b) between two RNA-binding domains. Here we show D3b binds full...

journal_title：Virology

authors： Lutz L,Okenka G,Schoelz J,Leisner S

更新日期：2015-02-01 00:00:00

abstract：:Adenoviruses (Ads) infect hosts from all vertebrate species and have been investigated as vaccine vectors. We report here near-atomic structures of two bovine Ad type 3 (BAd3) intermediates obtained by cryo-electron microscopy. A comparison between the two intermediate structures reveals that the differences are local...

journal_title：Virology

authors： Cheng L,Huang X,Li X,Xiong W,Sun W,Yang C,Zhang K,Wang Y,Liu H,Huang X,Ji G,Sun F,Zheng C,Zhu P

更新日期：2014-02-01 00:00:00

abstract：:We used a mouse nasal model of herpes simplex virus 2 (HSV-2) infection to examine the biological properties of HSV-2 wild-type (wt), TK-negative, and replication-defective strains in vivo. Nasal septa tissue is the major site of wt viral replication post intranasal (i.n.) inoculation. The HSV-2 strain 186 syn(+)-1 wt...

journal_title：Virology

authors： Jones CA,Taylor TJ,Knipe DM

更新日期：2000-12-05 00:00:00

abstract：:Segmented genomes of RNA viruses are thought to evolve and be maintained in analogy to sexual recombination and reassortment in eukaryotic systems. If reassortment among genomes is an important event in cucumoviral evolution, then such events should be detectable among extant viruses. In this study, phylogenetic analy...

journal_title：Virology

authors： White PS,Morales F,Roossinck MJ

更新日期：1995-02-20 00:00:00

abstract：:Interleukin (IL)-15, is a cytokine that is important for the maintenance of long-lasting, high-avidity T cell response to invading pathogens and has, therefore, been used in vaccine and therapeutic platforms as an adjuvant. In addition to pure protein delivery, plasmids encoding the IL-15 gene have been utilized. Howe...

journal_title：Virology

authors： Yin J,Dai A,Laddy DJ,Yan J,Arango T,Khan AS,Lewis MG,Andersen H,Kutzler MA,Draghia-Akli R,Weiner DB,Boyer JD

更新日期：2009-10-10 00:00:00

abstract：:Cell lines persistently infected with foot-and-mouth disease virus (FMDV) have been established by growth of BHK-21 (c-13) or IBRS-2 (c-26) that survived standard cytolytic infections with FMDV. They maintain cytoplasmic FMDV RNA sequences, as shown by dot blot hybridization tests, using cloned FMDV cDNA as probes. Ce...

journal_title：Virology

authors： de la Torre JC,Dávila M,Sobrino F,Ortín J,Domingo E

更新日期：1985-08-01 00:00:00

abstract：:Functions of the six Epstein-Barr virus latent infection nuclear proteins (EBNA-1, -2, -3A, -3B, -3C, or -LP) in maintaining latent infection or cell growth transformation are only partially understood. Using antibodies specific for each EBNA in immunofluorescence microscopy, EBNA-2, -3A, and -3C localized to subnucle...

journal_title：Virology

authors： Petti L,Sample C,Kieff E

更新日期：1990-06-01 00:00:00

abstract：:Herpesvirus glycoprotein C (gC) functions as a major virus attachment protein. The gC sequence of the neurovirulent bovine herpesvirus type 5 (BHV-5) virus was determined and compared with the gC sequence of the nonneurovirulent BHV-1. Alignment of the predicted amino acid sequences of BHV-1 and BHV-5 gC ORFs showed t...

journal_title：Virology

authors： Chowdhury SI

更新日期：1995-11-10 00:00:00

abstract：:When hemagglutinin (HA) and neuraminidase (NA) are presented together on an intact influenza virus particle, the antigens are competitive, with HA dominant over NA in T- and B-cell priming. Immunization with mixtures of purified HA and NA eliminates antigenic competition between HA and NA, as well as between N1-N2 NA ...

journal_title：Virology

authors： Johansson BE,Kilbourne ED

更新日期：1996-11-01 00:00:00

abstract：:Purified glycophorin (predominantly type A) from human erythrocytes was found to effectively inhibit reovirus hemagglutination (HA) in contrast to other glycoproteins such as fetuin or ovalbumin. Glycophorin was also a potent inhibitor of reovirus and protein sigma 1 binding to mouse L fibroblasts. Glycophorin pretrea...

journal_title：Virology

authors： Paul RW,Lee PW

更新日期：1987-07-01 00:00:00

abstract：:Open reading frames identified on the four genomic RNAs of beet necrotic yellow vein virus were cloned into bacterial expression vectors and resulting cl-fusion proteins expressed in Escherichia coli were used to raise polyclonal antibodies. This set of antisera was used to show the presence of 7 of 9 predicted viral ...

journal_title：Virology

authors： Niesbach-Klösgen U,Guilley H,Jonard G,Richards K

更新日期：1990-09-01 00:00:00

abstract：:Animal viruses profoundly modify the metabolism of lipids, the synthesis of new membranes, and membrane traffic. These alterations are related to the replication of viral genomes. Addition of oleic acid from the beginning of poliovirus infection inhibits the appearance of virus polypeptides at concentrations that do n...

journal_title：Virology

authors： Guinea R,Carrasco L

更新日期：1991-11-01 00:00:00

abstract：:Astrocytes protect neurons but also evoke a proinflammatory response to injury and viral infections including HIV. We investigated the mechanism of HIV-1 infection in primary astrocytes, which showed minimal but productive viral infection independent of CXCR4. As with ectopic-CD4-expressing astrocytes, lysosomotropic ...

journal_title：Virology

authors： Chauhan A,Mehla R,Vijayakumar TS,Handy I

更新日期：2014-05-01 00:00:00

abstract：:West Nile virus (WNV) is a flavivirus that swept rapidly across North America in 1999, declined in prevalence, and then resurged in 2012. To date, no vaccine is available to prevent infection in the human population. Herpes simplex virus (HSV) replication-defective vaccine vectors induce a durable immunity characteriz...

journal_title：Virology

authors： Taylor TJ,Diaz F,Colgrove RC,Bernard KA,DeLuca NA,Whelan SPJ,Knipe DM

更新日期：2016-09-01 00:00:00

abstract：:The interaction of reovirus with the cytoskeleton was investigated. The soluble components of infected cells were extracted with the nonionic detergent NP-40 in a physiological buffer, and a cytoskeletal extract was prepared from the detergent-insoluble fraction. We observed a selective association of viral-specified ...

journal_title：Virology

authors： Mora M,Partin K,Bhatia M,Partin J,Carter C

更新日期：1987-08-01 00:00:00

abstract：:A human B-lymphoid cell line, designated TG8, which does not express detectable levels of the EBV (Epstein-Barr virus)-associated nuclear antigen (EBNA), yet carries an average of one to two plasmid copies of the P3HR-1 EBV genome has been identified. TG8 can be superinfected by B95-8 EBV, resulting in up to 60-70% of...

journal_title：Virology

authors： Reisman D,Sugden B

更新日期：1984-08-01 00:00:00

abstract：:Marburg virus (MBGV), for which no vaccines or treatments currently exist, causes an acute hemorrhagic fever with a high mortality rate in humans. We previously showed that immunization with either killed MBGV or a glycoprotein (GP) subunit prevented lethal infection in guinea pigs. In the studies reported here, an RN...

journal_title：Virology

authors： Hevey M,Negley D,Pushko P,Smith J,Schmaljohn A

更新日期：1998-11-10 00:00:00

abstract：:We cloned and sequenced the cDNAs against genomic RNAs and mRNAs for phosphoproteins (Ps) of human parainfluenza virus types 4A (PIV-4A) and 4B (PIV-4B). The PIV-4A and -4B P genes were 1535 nucleotides including poly(A) tract and were found to have two small open reading frames, neither of which was apparently large ...

journal_title：Virology

authors： Kondo K,Bando H,Tsurudome M,Kawano M,Nishio M,Ito Y

更新日期：1990-09-01 00:00:00

abstract：:Simian cells permissive for influenza A virus infection were stably transformed with a full-length cloned influenza A nucleoprotein gene under the control of an inducible metallothionein promoter and linked to a dihydrofolate reductase gene to facilitate cell selection. Transformed cells synthesized a virus-specific n...

journal_title：Virology

authors： Ryan KW,Mackow ER,Chanock RM,Lai CJ

更新日期：1986-10-15 00:00:00

abstract：:A number of studies examining interactions of dendritic cell (DC)-specific ICAM-3 grabbing nonintegrin (DC-SIGN) with viral pathogens have relied on monocytic transfectants as models for primary DCs. Here we show that the presumed "THP-1" monocytic cells used in these studies are instead Raji B cells. Moreover, we dem...

journal_title：Virology

authors： Wu L,Martin TD,Carrington M,KewalRamani VN

更新日期：2004-01-05 00:00:00

abstract：:The complete DNA sequence of a new lytic T7-like bacteriophage phiKMV is presented. It is the first genome sequence of a member of the Podoviridae that infects Pseudomonas aeruginosa. The linear G + C-rich (62.3%) double-stranded DNA genome of 42,519 bp has direct terminal repeats of 414 bp and contains 48 open readin...

journal_title：Virology

authors： Lavigne R,Burkal'tseva MV,Robben J,Sykilinda NN,Kurochkina LP,Grymonprez B,Jonckx B,Krylov VN,Mesyanzhinov VV,Volckaert G

更新日期：2003-07-20 00:00:00

abstract：:This study evaluates the role of antibody as an indicator of immunity to ocular challenge with herpes simplex virus (HSV). Two genotypes of mice, BALB/c or BALB/c with mu-chain knockout (muK/O; which lack functional B cells), were immunized systemically either with nonvirulent infectious virus or with a eukaryotic exp...

journal_title：Virology

authors： Daheshia M,Deshpande S,Chun S,Kuklin NA,Rouse BT

更新日期：1999-04-25 00:00:00

abstract：:Twelve avian reovirus strain 176 proteins, 10 structural (lambda 1, lambda 2, lambda 3, mu 1, mu 2/mu 2C, sigma 1, sigma 2, sigma 3, and sigma 4), and 2 nonstructural (mu NS and sigma NS) were identified and characterized by electrophoretic analysis of purified virions and infected cell lysates. Three of the identifie...

journal_title：Virology

authors： Ni Y,Ramig RF,Kemp MC

更新日期：1993-03-01 00:00:00

abstract：:Neutralizing antibodies (Nabs) are thought to play an important role in prevention and control of HIV-1 infection and should be targeted by an AIDS vaccine. It is critical to understand how HIV-1 induces Nabs by analyzing viral sequences in both tested viruses and sera. Neutralization susceptibility to antibodies in a...

journal_title：Virology

authors： Kirchherr JL,Hamilton J,Lu X,Gnanakaran S,Muldoon M,Daniels M,Kasongo W,Chalwe V,Mulenga C,Mwananyanda L,Musonda RM,Yuan X,Montefiori DC,Korber BT,Haynes BF,Gao F

更新日期：2011-01-20 00:00:00

abstract：:To investigate the role of Tobacco vein banding mosaic virus (TVBMV) 3'-UTR in virus systemic infection, three types of deletions were introduced into TVBMV infectious clone pCaTVBMV-GFP. Mutants with deletions at the nucleotide position 8-42, 43-141, or 163-174 in the 3'-UTR failed to cause systemic infection in N. b...

journal_title：Virology

authors： Wang Y,Yang ZY,Tian YP,Geng C,Yuan XF,Li XD

更新日期：2019-01-15 00:00:00

abstract：:While DNA vaccines have been shown to prime cellular immune responses, levels are often low in nonhuman primates or humans. Hence, efforts have been directed toward boosting responses by combining DNA with different vaccination modalities. To this end, a polyvalent DNA prime/protein boost vaccine, consisting of codon ...

journal_title：Virology

authors： Cristillo AD,Wang S,Caskey MS,Unangst T,Hocker L,He L,Hudacik L,Whitney S,Keen T,Chou TH,Shen S,Joshi S,Kalyanaraman VS,Nair B,Markham P,Lu S,Pal R

更新日期：2006-03-01 00:00:00

abstract：:Without a virus culture system, genetic analysis becomes the principal method to classify norovirus (NoV) strains. Currently, classification of NoV strains beneath the species level has been based on sequences from different regions of the viral genome. As a result, the phylogenetic insights of some virus were not app...

journal_title：Virology

authors： Zheng DP,Ando T,Fankhauser RL,Beard RS,Glass RI,Monroe SS

更新日期：2006-03-15 00:00:00

abstract：:We have previously described a revertant cell line which expresses a dominant tumor suppressor phenotype to E1 but not to heterologous oncogenes such as c-myc, N-ras, or polyoma middle t (Sircar et al. (1988) Oncogene 3, 725-728). DNA tumor virus oncogenes have been suggested to transform cells via the common mechanis...

journal_title：Virology

authors： Sircar S,Horvath J,Roberge D,Diouri M,Weber JM

更新日期：1992-11-01 00:00:00

abstract：:It has been shown that the genomes of episomally maintained DNA viruses are tethered to host cell chromosomes during cell division, facilitating maintenance in dividing cells. The papillomavirus E2 protein serves this mechanism of viral genome persistence by simultaneously associating with chromatin and the viral geno...

journal_title：Virology

authors： Feeney KM,Saade A,Okrasa K,Parish JL

更新日期：2011-05-25 00:00:00

abstract：:Some satellite (sat-) and defective interfering (DI) RNAs associated with plant viruses intensify or ameliorate the symptoms of the virus. We recently demonstrated that the TCV coat protein (CP) is involved in symptom modulation by sat-RNA C. Two additional subviral RNAs have now been tested for effect of the CP on sy...

journal_title：Virology

authors： Kong Q,Oh JW,Carpenter CD,Simon AE

更新日期：1997-11-24 00:00:00