Preclinical evaluation of cellular immune responses elicited by a polyvalent DNA prime/protein boost HIV-1 vaccine.


:While DNA vaccines have been shown to prime cellular immune responses, levels are often low in nonhuman primates or humans. Hence, efforts have been directed toward boosting responses by combining DNA with different vaccination modalities. To this end, a polyvalent DNA prime/protein boost vaccine, consisting of codon optimized HIV-1 env (A, B, C, E) and gag (C) and homologous gp120 proteins in QS-21, was evaluated in rhesus macaques and BALB/c mice. Humoral and cellular responses, detected following DNA immunization, were increased following protein boost in macaques and mice. In dissecting cellular immune responses in mice, protein-enhanced responses were found to be mediated by CD4+ and CD8+ T cells with a Th1 cytokine bias. Our study reveals that, in addition to augmenting humoral responses, protein boosting of DNA-primed animals augments cellular immune responses mediated by CD8+ CTL, CD4+ T-helper cells and Th1 cytokines; thus, offering much promise in controlling HIV-1 in vaccinees.






Cristillo AD,Wang S,Caskey MS,Unangst T,Hocker L,He L,Hudacik L,Whitney S,Keen T,Chou TH,Shen S,Joshi S,Kalyanaraman VS,Nair B,Markham P,Lu S,Pal R




Has Abstract


2006-03-01 00:00:00
















  • Analysis of a type D retroviral capsid gene expressed in ovine pulmonary carcinoma and present in both affected and unaffected sheep genomes.

    abstract::Ovine pulmonary carcinoma is a contagious neoplastic disease of sheep which can be transmitted experimentally with cell-free filtrate. Products homologous to structural proteins of type D and type B retroviruses are expressed in the tumors, but an infectious retrovirus has yet to be isolated. Retroviral RNA was isolat...


    pub_type: 杂志文章


    authors: Hecht SJ,Carlson JO,DeMartin JC

    更新日期:1994-07-01 00:00:00

  • Infection of mononucleated phagocytes with human cytomegalovirus.

    abstract::Human cytomegalovirus (HCMV) can be isolated from peripheral blood leukocytes; however, in vitro, only abortive infection of monocytes, lymphocytes, and granulocytes has been detected. These studies demonstrate that freshly isolated monocytes can be infected with HCMV. Infection of monocytes was not associated with lo...


    pub_type: 杂志文章


    authors: Maciejewski JP,Bruening EE,Donahue RE,Sellers SE,Carter C,Young NS,St Jeor S

    更新日期:1993-08-01 00:00:00

  • Attenuation of Marek's disease virus by codon pair deoptimization of a core gene.

    abstract::Marek's disease virus (MDV) is an oncogenic alphaherpesvirus of Gallus gallus, the domesticated chicken. Control strategies rely upon vaccination with live attenuated viruses of antigenically similar avian herpesviruses or attenuated strains of MDV. Recent studies in other viruses have shown that recoding certain vira...


    pub_type: 杂志文章


    authors: Conrad SJ,Silva RF,Hearn CJ,Climans M,Dunn JR

    更新日期:2018-03-01 00:00:00

  • Packaging of AeDNV-GFP transducing virus by expression of densovirus structural proteins from a sindbis virus expression system.

    abstract::Genetic recombination resulting in the production of wild-type infectious virus is an obstacle in the current system for producing densovirus transducing particles. In order to eliminate this problem, a double subgenomic Sindbis virus (TE/3'2J/VP) was engineered that expresses the structural proteins (VPs) of Aedes de...


    pub_type: 杂志文章


    authors: Allen-Miura TM,Afanasiev BN,Olson KE,Beaty BJ,Carlson JO

    更新日期:1999-04-25 00:00:00

  • A single amino acid substitution in the hemagglutinin-neuraminidase of Newcastle disease virus results in a protein deficient in both functions.

    abstract::Sequence determinations of the hemagglutinin-neuraminidase (HN) glycoproteins of a temperature-sensitive mutant of Newcastle disease virus and two sequentially selected revertants had previously shown that substitution at a pair of residues, 129 and 175, resulted in a deficiency in neuraminidase (NA) activity, which w...


    pub_type: 杂志文章


    authors: Sheehan JP,Iorio RM

    更新日期:1992-08-01 00:00:00

  • The M4 gene of gammaHV68 encodes a secreted glycoprotein and is required for the efficient establishment of splenic latency.

    abstract::Sequence analysis of the murine gamma-herpesvirus 68 (gammaHV68) genome previously identified several open reading frames (ORFs) located at the left end of the viral genome that do not share homology with other known herpesvirus or cellular genes. Here, we show that one of these ORFs, M4, encodes a secreted glycoprote...


    pub_type: 杂志文章


    authors: Evans AG,Moorman NJ,Willer DO,Speck SH

    更新日期:2006-01-20 00:00:00

  • Establishment of cell lines persistently infected with foot-and-mouth disease virus.

    abstract::Cell lines persistently infected with foot-and-mouth disease virus (FMDV) have been established by growth of BHK-21 (c-13) or IBRS-2 (c-26) that survived standard cytolytic infections with FMDV. They maintain cytoplasmic FMDV RNA sequences, as shown by dot blot hybridization tests, using cloned FMDV cDNA as probes. Ce...


    pub_type: 杂志文章


    authors: de la Torre JC,Dávila M,Sobrino F,Ortín J,Domingo E

    更新日期:1985-08-01 00:00:00

  • Association of histone deacetylase with COUP-TF in tumorigenic Ad12-transformed cells and its potential role in shut-off of MHC class I transcription.

    abstract::Chicken ovalbumin upstream promoter-transcription factor (COUP-TF) is an orphan nuclear receptor that represses transcription of many genes. In adenovirus type 12 (Ad12) transformed cells, a high level of binding activity of COUP-TF to the major histocompatibility complex (MHC) class I enhancer correlates with the dow...


    pub_type: 杂志文章


    authors: Smirnov DA,Hou S,Ricciardi RP

    更新日期:2000-03-15 00:00:00

  • Factors that affect genetic interaction during mixed infection with temperature-sensitive mutants of simian rotavirus SA11.

    abstract::A number of factors that affect genetic interaction during mixed infection with temperature-sensitive mutants of simian rotavirus SA11 have been examined. (1) Statistical analyses of recombination frequency (RF) indicated that (a) the variability noted in RF was not related to variations in experimental conditions and...


    pub_type: 杂志文章


    authors: Ramig RF

    更新日期:1983-05-01 00:00:00

  • Insights into the interaction between tRNA and primer binding site from characterization of a unique HIV-1 virus which stably maintains dual PBS complementary to tRNA(Gly) and tRNA(His).

    abstract::Previously our laboratory constructed an HIV-1 which stably maintained a primer binding site (PBS) complementary to tRNA(His) by mutating the region of the provirus within U5 postulated to interact with the anticodon of tRNA(His) (J. Wakefield, S-M Kang, and C. D. Morrow, 1996, J. Virol, 70, 966-975). From the analysi...


    pub_type: 杂志文章


    authors: Li Y,Zhang Z,Kang SM,Buescher JL,Morrow CD

    更新日期:1997-11-24 00:00:00

  • A new DNA binding protein highly conserved in diverse crenarchaeal viruses.

    abstract::Sulfolobus turreted icosahedral virus (STIV) infects Sulfolobus species found in the hot springs of Yellowstone National Park. Its 37 open reading frames (ORFs) generally lack sequence similarity to other genes. One exception, however, is ORF B116. While its function is unknown, orthologs are found in three additional...


    pub_type: 杂志文章


    authors: Larson ET,Eilers BJ,Reiter D,Ortmann AC,Young MJ,Lawrence CM

    更新日期:2007-07-05 00:00:00

  • Identification of the 5' end of the rubella virus subgenomic RNA.

    abstract::The 5' end of the subgenomic RNA of rubella virus was determined by primer extension. The Maxam-Gilbert sequence ladder of the primer extension product contained a determinable sequence which was colinear with the complement of the sequence of the genomic RNA through nucleotide 3325 from the 3' end of the genomic RNA ...


    pub_type: 杂志文章


    authors: Frey TK,Marr LD,Sanchez A,Simmons RB

    更新日期:1989-01-01 00:00:00

  • The secretory cells of mouse salivary glands are nonpermissive for polyomavirus replication.

    abstract::Parotid tumor agent was an early name for polyomavirus due to its ability to induce tumors (myoepitheliomas originating from the myoepithelial glandular tissues) in mice inoculated with polyomavirus (Py) as neonates. It has long been thought that these tumors directly follow productive infection by Py in epithelial ce...


    pub_type: 杂志文章


    authors: Atencio IA,Meraz A,Villarreal LP

    更新日期:1994-05-01 00:00:00

  • Apoptosis correlates with immune activation in intestinal lymphoid tissue from macaques acutely infected by a highly enteropathic simian immunodeficiency virus, SIVsmmPBj14.

    abstract::The PBj14 isolate of simian immunodeficiency virus, SIVsmmPBj14, induces an acutely lethal disease in experimentally inoculated pigtailed macaques, that is characterized by severe enteropathy and extensive immune activation, particularly within gut-associated lymphoid tissue (GALT). Experiments were conducted to deter...


    pub_type: 杂志文章


    authors: Gummuluru S,Novembre FJ,Lewis M,Gelbard HA,Dewhurst S

    更新日期:1996-11-01 00:00:00

  • Characterization of a 105-kDa plasma membrane associated glycoprotein that is involved in West Nile virus binding and infection.

    abstract::This study attempts to isolate and characterize West Nile virus-binding molecules on the plasma membrane of Vero and murine neuroblastoma cells that is responsible for virus entry. Pretreatment of Vero cells with proteases, glycosidases (endoglycosidase H, alpha-mannosidase), and sodium periodate strongly inhibited We...


    pub_type: 杂志文章


    authors: Chu JJ,Ng ML

    更新日期:2003-08-01 00:00:00

  • Sulfated polymers inhibit the interaction of human cytomegalovirus with cell surface heparan sulfate.

    abstract::Several sulfated polysaccharides (dextran sulfate, pentosan polysulfate, heparin) and copolymers of acrylic acid with vinylalcohol sulfate have proved to be potent inhibitors of human cytomegalovirus (CMV) infectivity in vitro. Sulfated alpha-cyclodextrins are only weak inhibitors of CMV. A close correlation was found...


    pub_type: 杂志文章


    authors: Neyts J,Snoeck R,Schols D,Balzarini J,Esko JD,Van Schepdael A,De Clercq E

    更新日期:1992-07-01 00:00:00

  • The spiroplasma virus 4 replicative form cloned in Escherichia coli transfects spiroplasmas.

    abstract::The replicative form (RF) of spiroplasma virus 4 (SpV4) has been purified from infected cells of Spiroplasma melliferum strain G1 by alkaline lysis followed by low melting point agarose gel electrophoresis. A partial restriction map has been established. The circular RF was linearized by cutting at the unique ClaI res...


    pub_type: 杂志文章


    authors: Pascarel-Devilder MC,Renaudin J,Bove JM

    更新日期:1986-06-01 00:00:00

  • Glycoprotein C-dependent attachment of herpes simplex virus to susceptible cells leading to productive infection.

    abstract::Herpes simplex viruses encode several glycoproteins dispensable for infection and replication in cell culture. Evidence is presented that there exist at least two pathways for viral attachment to cells, i.e., one mediated by the dispensable glycoprotein C (gC) and one independent of that glycoprotein. Thus, whereas th...


    pub_type: 杂志文章


    authors: Campadelli-Fiume G,Stirpe D,Boscaro A,Avitabile E,Foá-Tomasi L,Barker D,Roizman B

    更新日期:1990-09-01 00:00:00

  • Glycosylation of the major outer capsid protein of bluetongue viruses.

    abstract::The major outer capsid protein, VP5, of five US bluetongue viruses, was found to be glycosylated. Enzymatic removal of the carbohydrate moiety did not affect the electrophoretic mobility of VP5 in SDS-PAGE, indicating the presence of only short and possibly unbranched oligosaccharide chains. The surface accessibilitie...


    pub_type: 杂志文章


    authors: Yang YY,Li JK

    更新日期:1993-05-01 00:00:00

  • Complete genome sequence of bacteriophage T5.

    abstract::The 121,752-bp genome sequence of bacteriophage T5 was determined; the linear, double-stranded DNA is nicked in one of the strands and has large direct terminal repeats of 10,139 bp (8.3%) at both ends. The genome structure is consistently arranged according to its lytic life cycle. Of the 168 potential open reading f...


    pub_type: 杂志文章


    authors: Wang J,Jiang Y,Vincent M,Sun Y,Yu H,Wang J,Bao Q,Kong H,Hu S

    更新日期:2005-02-05 00:00:00

  • Turnip crinkle virus infection from RNA synthesized in vitro.

    abstract::Genome-length cDNA clones of turnip crinkle virus (TCV) were constructed with SmaI and XbaI restriction sites engineered at the 5' and 3' termini, respectively. The genome-length cDNAs were positioned downstream of modified lambda and T7 phage promoters such that in vitro transcription resulted in RNAs with 5 extra nu...


    pub_type: 杂志文章


    authors: Heaton LA,Carrington JC,Morris TJ

    更新日期:1989-05-01 00:00:00

  • Sequence analysis of the genome of porcine lymphotropic herpesvirus 1 and gene expression during posttransplant lymphoproliferative disease of pigs.

    abstract::The porcine lymphotropic herpesvirus 1 (PLHV-1), the first gammaherpesvirus of pigs, has been detected at a high prevalence in healthy pig populations. A porcine gammaherpesvirus has also been detected at high copy numbers in animals suffering from posttransplant lymphoproliferative disease (PTLD). While human PTLD is...


    pub_type: 杂志文章


    authors: Goltz M,Ericsson T,Patience C,Huang CA,Noack S,Sachs DH,Ehlers B

    更新日期:2002-03-15 00:00:00

  • Quasispecies variation of porcine reproductive and respiratory syndrome virus during natural infection.

    abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) displays notorious genetic, antigenic, and clinical variability. Little is known, however, about the nature and extent of viral variation present within naturally infected animals. By amplifying and cloning the open reading frame 5 gene from tonsils of natura...


    pub_type: 杂志文章


    authors: Goldberg TL,Lowe JF,Milburn SM,Firkins LD

    更新日期:2003-12-20 00:00:00

  • Human papillomavirus types 16 E6 and E7 contribute differently to carcinogenesis.

    abstract::High-risk human papillomaviruses (HPVs) are etiologically implicated in human cervical cancer. Two viral genes, E6 and E7, are commonly found expressed in these cancer cells. We have previously shown that mice transgenic for the HPV-16 E6 gene or E7 gene, in which the E6 or E7 was expressed in the basal layer of epith...


    pub_type: 杂志文章


    authors: Song S,Liem A,Miller JA,Lambert PF

    更新日期:2000-02-15 00:00:00

  • Identification of adenovirus E1A regions which affect MHC class I expression and susceptibility to cytotoxic T lymphocytes.

    abstract::To map and characterize functional differences between the E1A oncoproteins of Ad5 and Ad12, we previously constructed a series of hybrid Ad5/12 E1A genes and used them in combination with Ad12 E1B to transform Hooded Lister rat cells. At least two regions within the first exon of Ad12 E1A which influenced tumorigenic...


    pub_type: 杂志文章


    authors: Pereira DS,Rosenthal KL,Graham FL

    更新日期:1995-08-01 00:00:00

  • Analysis of rubella virus E1 glycosylation mutants expressed in COS cells.

    abstract::cDNA clones encoding the envelope glycoprotein E1 of rubella virus (RV) were altered by site-directed mutagenesis at consensus sites for addition of N-linked glycans. The resulting plasmids were introduced into COS cells and the mutant E1 proteins were analyzed by indirect immunofluorescence, radioimmunoprecipitation,...


    pub_type: 杂志文章


    authors: Hobman TC,Qiu ZY,Chaye H,Gillam S

    更新日期:1991-04-01 00:00:00

  • Modeling-based characterization of the elicitor function of amino acid 461 of Cucumber mosaic virus 1a protein in the hypersensitive response.

    abstract::The Ns strain of Cucumber mosaic virus (CMV) induces hypersensitive response (HR) on Nicotiana tabacum cv. Xanthi-nc and on Nicotiana glutinosa. The genetic determinant of the HR induction was localized earlier to amino acid 461 of the 1a protein. The 3D structure of the 1a protein is still unknown and building a homo...


    pub_type: 杂志文章


    authors: Salánki K,Gellért A,Náray-Szabó G,Balázs E

    更新日期:2007-02-05 00:00:00

  • The measles virus phosphoprotein interacts with the linker domain of STAT1.

    abstract::The measles virus (MV) phosphoprotein (P) and V proteins block the interferon (IFN) response by impeding phosphorylation of the signal transducer and activator of transcription 1 (STAT1) by the Janus kinase 1 (JAK1). We characterized how STAT1 mutants interact with P and JAK1 phosphorylation. Certain mutants of the li...


    pub_type: 杂志文章


    authors: Devaux P,Priniski L,Cattaneo R

    更新日期:2013-09-01 00:00:00

  • Herpes simplex virus-induced "rolling circle" amplification of SV40 DNA sequences in a transformed hamster cell line correlates with tandem integration of the SV40 genome.

    abstract::Infection with herpes simplex virus leads to amplification of SV40 DNA in various SV40-transformed cells. In earlier studies with the SV40-transformed hamster cell line Elona two different types of DNA amplification could be identified: (i) Bidirectional overreplication of chromosomally integrated SV40 DNA expanding i...


    pub_type: 杂志文章


    authors: Gerspach R,Matz B

    更新日期:1989-12-01 00:00:00

  • Molecular characterization of Drosophila cells persistently infected with Flock House virus.

    abstract::Little is known about the molecular determinants causing and sustaining viral persistent infections at the cellular level. We found that Drosophila cells persistently infected (PI) with Flock House virus (FHV) invariably harbor defective viral RNAs, which are replicated by the FHV RNA-dependent RNA polymerase. Some de...


    pub_type: 杂志文章


    authors: Jovel J,Schneemann A

    更新日期:2011-10-10 00:00:00