Abstract:
:Tombusviruses, of which tomato bushy stunt virus (TBSV) is the type member, spontaneously generate defective interfering RNAs (DIs) that are known to interfere with viral accumulation and symptom development. We show that co-infection with TBSV and DIs causes a dramatic reduction in accumulation of TBSV subgenomic RNAs and corresponding TBSV proteins with a less dramatic reduced accumulation of the genomic RNA and the replicase proteins. Associated with this differentially regulated suppression was a greatly reduced expression of both the p19 protein, which is responsible for severe symptoms, and the p22 protein, which is associated with cell-to-cell movement of the virus. Therefore, the results suggest that the protective effect of DIs may be due to selective inhibition of p19 and p22 expression in addition to reduced replication of genomic RNA.
journal_name
Virologyjournal_title
Virologyauthors
Scholthof KB,Scholthof HB,Jackson AOdoi
10.1006/viro.1995.1410subject
Has Abstractpub_date
1995-08-01 00:00:00pages
324-8issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(85)71410-9journal_volume
211pub_type
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