The effect of defective interfering RNAs on the accumulation of tomato bushy stunt virus proteins and implications for disease attenuation.


:Tombusviruses, of which tomato bushy stunt virus (TBSV) is the type member, spontaneously generate defective interfering RNAs (DIs) that are known to interfere with viral accumulation and symptom development. We show that co-infection with TBSV and DIs causes a dramatic reduction in accumulation of TBSV subgenomic RNAs and corresponding TBSV proteins with a less dramatic reduced accumulation of the genomic RNA and the replicase proteins. Associated with this differentially regulated suppression was a greatly reduced expression of both the p19 protein, which is responsible for severe symptoms, and the p22 protein, which is associated with cell-to-cell movement of the virus. Therefore, the results suggest that the protective effect of DIs may be due to selective inhibition of p19 and p22 expression in addition to reduced replication of genomic RNA.






Scholthof KB,Scholthof HB,Jackson AO




Has Abstract


1995-08-01 00:00:00
















  • The cytoplasmic tail of Herpes simplex virus glycoprotein H binds to the tegument protein VP16 in vitro and in vivo.

    abstract::During Herpes simplex virus envelopment, capsids, tegument polypeptides, and membrane proteins assemble at the site of budding and a cellular lipid bilayer becomes refashioned into a spherical envelope. Though the molecular interactions driving these events are poorly understood, several lines of evidence suggest that...


    pub_type: 杂志文章


    authors: Gross ST,Harley CA,Wilson DW

    更新日期:2003-12-05 00:00:00

  • Characterization of human papillomavirus type 6b DNA isolated from an invasive squamous carcinoma of the vulva.

    abstract::Human papillomavirus type 6 (HPV 6) DNA was detected in an episomal form in DNA extracted from an invasive squamous carcinoma of the vulva. The viral DNA, designated HPV6-T70, was molecularly cloned. Restriction analysis of the HPV6-T70 genome revealed an insertion of approximately 35 bp in the 5' portion of the upstr...


    pub_type: 杂志文章


    authors: Kasher MS,Roman A

    更新日期:1988-07-01 00:00:00

  • Sequences within the early and late promoters of archetype JC virus restrict viral DNA replication and infectivity.

    abstract::Two forms of JC virus (JCV) have been isolated from its human host, an archetype found in kidney tissue and urine of nonimmunocompromised individuals and a rearranged type detected in lymphocytes and brain tissue of patients with and without progressive multifocal leukoencephalopathy. To investigate the hypothesis tha...


    pub_type: 杂志文章


    authors: Daniel AM,Swenson JJ,Mayreddy RP,Khalili K,Frisque RJ

    更新日期:1996-02-01 00:00:00

  • Segmental configuration and putative origin of the reassortant orbivirus, epizootic hemorrhagic disease virus serotype 6, strain Indiana.

    abstract::In 2006, an exotic reassortant orbivirus, epizootic hemorrhagic disease virus serotype 6 (EHDV-6) [strain (Indiana)], was first detected in the United States. To characterize the reassortment configuration of this virus and to conclusively determine the parental virus of each RNA segment, the complete genome of EHDV-6...


    pub_type: 杂志文章


    authors: Allison AB,Holmes EC,Potgieter AC,Wright IM,Sailleau C,Breard E,Ruder MG,Stallknecht DE

    更新日期:2012-03-01 00:00:00

  • Construction and characterization of an infectious clone generated from Chikungunya virus SL11131 strain.

    abstract::Chikungunya virus (CHIKV) is a mosquito-borne RNA virus that causes Chikungunya fever in humans. In this study, we generated two DNA-based CHIKV infectious clones derived from an Indian Ocean Lineage SL11131 strain and a prototype Ross strain. When the replication capabilities of the infectious CHIKV in various cell l...


    pub_type: 杂志文章


    authors: Suzuki Y,Tanaka A,Maeda Y,Emi A,Fujioka Y,Sakaguchi S,Vasudevan SG,Kobayashi T,Lim CK,Takasaki T,Wu H,Nakano T

    更新日期:2021-01-02 00:00:00

  • Antiviral activity of natural and recombinant human leukocyte interferons in tobacco protoplasts.

    abstract::Several purified species of human leukocyte interferon, including recombinant interferons, inhibit the multiplication of tobacco mosaic virus (TMV) in tobacco protoplasts derived from various cultivars. Viral RNA accumulation was determined by dot-blot hybridization to specific cDNA probes, and virus antigen was deter...


    pub_type: 杂志文章


    authors: Rosenberg N,Reichman M,Gera A,Sela I

    更新日期:1985-01-15 00:00:00

  • Nucleotide sequence of the 26 S mRNA of the virulent Trinidad donkey strain of Venezuelan equine encephalitis virus and deduced sequence of the encoded structural proteins.

    abstract::A cDNA clone containing all of the 26 S mRNA coding region of the RNA genome of Venezuelan equine encephalitis (VEE) virus, virulent strain Trinidad donkey (TRD), has been constructed and sequenced. The nucleotide and deduced amino acid sequences of the 26 S RNA of VEE virus conform to the general organization of the ...


    pub_type: 杂志文章


    authors: Kinney RM,Johnson BJ,Brown VL,Trent DW

    更新日期:1986-07-30 00:00:00

  • Antigenic mimicry of an immunoglobulin A epitope by a hepatitis B virus cell attachment site.

    abstract::The preS(21-47) sequence of the hepatitis B virus (HBV) envelope protein is involved in binding of the virus to cell receptors. A protein similarity search revealed a partial homology between this sequence and a segment of the human immunoglobulin A (IgA) heavy chain constant region, suggesting that the cell attachmen...


    pub_type: 杂志文章


    authors: Neurath AR,Strick N

    更新日期:1990-10-01 00:00:00

  • Simian virus 40 Vp2/3 small structural proteins harbor their own nuclear transport signal.

    abstract::We have used a microinjection approach to identify a domain of the simian virus 40 (SV40) structural proteins Vp2 and Vp3(Vp2/3) responsible for their nuclear transport. By using both synthetic peptides, containing small regions of Vp2/3 conjugated to bovine serum albumin (BSA), and beta-galactosidase-Vp3 fusion prote...


    pub_type: 杂志文章


    authors: Clever J,Kasamatsu H

    更新日期:1991-03-01 00:00:00

  • Identification and analysis of an Autographa californica nuclear polyhedrosis virus structural protein of the occlusion-derived virus envelope: ODV-E56.

    abstract::An Autographa californica nuclear polyhedrosis virus gene encoding an occlusion-derived virus (ODV) envelope protein of 56 kDa was identified and sequenced. Transcription initiates from a conserved baculovirus late motif (ATAAG) with transcripts detected from 16 through 72 hr p.i. The protein is detected in infected c...


    pub_type: 杂志文章


    authors: Braunagel SC,Elton DM,Ma H,Summers MD

    更新日期:1996-03-01 00:00:00

  • RNA segment 5 of broadhaven virus, a tick-borne orbivirus, shows sequence homology with segment 5 of bluetongue virus.

    abstract::The sequence of Broadhaven (BRD) virus segment 5, the major genetic determinant of serotype, is 1658 nucleotides in length and contains a single open reading frame (ORF) having the coding capacity for a protein of Mr 52.5K. Comparison of the ORF of segment 5 of BRD virus with published sequences of bluetongue virus (B...


    pub_type: 杂志文章


    authors: Moss SR,Fukusho A,Nuttall PA

    更新日期:1990-11-01 00:00:00

  • A conserved internal hydrophobic domain mediates the stable membrane integration of the dengue virus capsid protein.

    abstract::The mature flavivirus capsid protein (virion C) is commonly thought to be free in the cytoplasm of infected cells and to form a nucleocapsid-like complex with genomic RNA in mature virus particles. There is little sequence conservation among flavivirus virion C proteins, but they are similar in size (e.g., 99 amino ac...


    pub_type: 杂志文章


    authors: Markoff L,Falgout B,Chang A

    更新日期:1997-06-23 00:00:00

  • Circovirus in domestic and wild carnivores: An important opportunistic agent?

    abstract::Circoviruses are relatively novel pathogens with increased importance in canids. In this study, we first screened the presence of dog circovirus (DogCV) by molecular methods from a total number of 389 internal organ samples originating from 277 individuals of domestic dogs and wild animals including wolves, foxes and ...


    pub_type: 杂志文章


    authors: Zaccaria G,Malatesta D,Scipioni G,Di Felice E,Campolo M,Casaccia C,Savini G,Di Sabatino D,Lorusso A

    更新日期:2016-03-01 00:00:00

  • Nematode transmission of tobacco rattle virus serves as a bottleneck to clear the virus population from defective interfering RNAs.

    abstract::DI7 is a defective interfering RNA derived from RNA 2 of tobacco rattle tobravirus (TRV) isolate PpK20. Tobacco was transformed with DI7 cDNA fused to the CaMV 35S promoter. Upon infection of the transgenic plants with TRV isolate PpK20 or the serologically unrelated isolate PaY4, the transgenic DI7 RNA started to acc...


    pub_type: 杂志文章


    authors: Visser PB,Brown DJ,Brederode FT,Bol JF

    更新日期:1999-10-10 00:00:00

  • The high levels of p53 present in adenovirus early region 1-transformed human cells do not cause up-regulation of MDM2 expression.

    abstract::The cellular protein MDM2 can bind to the tumor suppressor gene product p53 and abrogate its transcriptional activity. In addition, p53 can regulate expression of the mdm2 gene. We and others have previously shown that p53 is present at high levels in adenovirus-transformed cells which express the larger E1B protein. ...


    pub_type: 杂志文章


    authors: Grand RJ,Lecane PS,Owen D,Grant ML,Roberts S,Levine AJ,Gallimore PH

    更新日期:1995-07-10 00:00:00

  • Hyper-phosphorylation of nsp2-related proteins of porcine reproductive and respiratory syndrome virus.

    abstract::Viruses exploit phosphorylation of both viral and host proteins to support viral replication. In this study, we demonstrate that porcine reproductive and respiratory syndrome virus replicase nsp2, and two nsp2-related -2/-1 frameshifting products, nsp2TF and nsp2N, are hyper-phosphorylated. By mapping phosphorylation ...


    pub_type: 杂志文章


    authors: Shang P,Yuan F,Misra S,Li Y,Fang Y

    更新日期:2020-04-01 00:00:00

  • AFV1, a novel virus infecting hyperthermophilic archaea of the genus acidianus.

    abstract::We describe a novel virus, AFV1, of the hyperthermophilic archaeal genus Acidianus. Filamentous virions are covered with a lipid envelope and contain at least five different proteins with molecular masses in the range of 23-130 kDa and a 20.8-kb-long linear double-stranded DNA. The virus has been assigned to the famil...


    pub_type: 杂志文章


    authors: Bettstetter M,Peng X,Garrett RA,Prangishvili D

    更新日期:2003-10-10 00:00:00

  • Capsid protein and helper component-proteinase function as potyvirus cell-to-cell movement proteins.

    abstract::The role of bean common mosaic necrosis potyvirus (BCMNV) and lettuce mosaic potyvirus (LMV) proteins was investigated in terms of their capacity to function as viral movement proteins (MPs). Using Escherichia coli-expressed proteins and microinjection techniques, direct evidence was obtained that both the potyviral c...


    pub_type: 杂志文章


    authors: Rojas MR,Zerbini FM,Allison RF,Gilbertson RL,Lucas WJ

    更新日期:1997-10-27 00:00:00

  • Transcriptional factor C/EBP binds to and transactivates the enhancer element II of the hepatitis B virus.

    abstract::The human hepatitis B Virus genome (HBV) contains a liver-specific enhancer upstream of the X ORF which has been studied in detail by several investigators. A second liver-specific enhancer element, designated here as enhancer II, has been relatively recently described in the HBV genome, which is located within the co...


    pub_type: 杂志文章


    authors: López-Cabrera M,Letovsky J,Hu KQ,Siddiqui A

    更新日期:1991-08-01 00:00:00

  • The nectin-1alpha transmembrane domain, but not the cytoplasmic tail, influences cell fusion induced by HSV-1 glycoproteins.

    abstract::Nectin-1 is a receptor for herpes simplex virus (HSV), a member of the immunoglobulin superfamily, and a cellular adhesion molecule. To study domains of nectin-1alpha involved in cell fusion, we measured the ability of nectin-1alpha/nectin-2alpha chimeras, nectin-1alpha/CD4 chimeras, and transmembrane domain and cytop...


    pub_type: 杂志文章


    authors: Subramanian RP,Dunn JE,Geraghty RJ

    更新日期:2005-09-01 00:00:00

  • Protection of guinea pigs from Lassa fever by vaccinia virus recombinants expressing the nucleoprotein or the envelope glycoproteins of Lassa virus.

    abstract::A recombinant vaccinia virus that expresses the nucleoprotein gene of Lassa virus (Josiah strain) under the control of the P7.5 promoter was constructed using the lacZ coexpression transfer vector pSC11. Southern blot analysis demonstrated that recombination of the sequences inserted within the thymidine kinase gene o...


    pub_type: 杂志文章


    authors: Morrison HG,Bauer SP,Lange JV,Esposito JJ,McCormick JB,Auperin DD

    更新日期:1989-07-01 00:00:00

  • Identification of two independent neutralization domains on the VP4 trypsin cleavage products VP5* and VP8* of human rotavirus ST3.

    abstract::The antigenic structure of the VP4 protein of human rotavirus (HRV) strains Wa and ST3 was studied by using a panel of Wa- and ST3-derived VP4-specific neutralizing monoclonal antibodies (NMAbs) and NMAb-resistant variants. The VP4-coding genes from three Wa and three ST3 variants were sequenced. For Wa VP4, one homot...


    pub_type: 杂志文章


    authors: Padilla-Noriega L,Dunn SJ,López S,Greenberg HB,Arias CF

    更新日期:1995-01-10 00:00:00

  • Cellular Hsp27 interacts with classical swine fever virus NS5A protein and negatively regulates viral replication by the NF-κB signaling pathway.

    abstract::Classical swine fever virus (CSFV) nonstructural protein NS5A is a multifunctional protein functioning in regulation of viral genome replication, protein translation and assembly by interaction with viral or host proteins. Here, heat shock protein 27 (Hsp27) has been identified as a novel binding partner of NS5A by us...


    pub_type: 杂志文章


    authors: Ling S,Luo M,Jiang S,Liu J,Ding C,Zhang Q,Guo H,Gong W,Tu C,Sun J

    更新日期:2018-05-01 00:00:00

  • Dispensability of 3' tRNA-like sequence for packaging cowpea chlorotic mottle virus genomic RNAs.

    abstract::The 3' ends of three genomic RNAs (gRNAs) of cowpea chlorotic mottle virus (CCMV) terminate in a highly conserved tRNA-like structure (3'TLS). To examine the intrinsic role played the 3'TLS in packaging, the competence of each gRNA lacking the 3' TLS (DeltaTLS-gRNA) to interact with dissociated coat protein (CP) subun...


    pub_type: 杂志文章


    authors: Annamalai P,Rao AL

    更新日期:2005-02-20 00:00:00

  • Isolation of a Friend recombinant polytropic virus with a T-cell-restricted leukemogenicity.

    abstract::Hematopoietic malignant cells of various types were isolated from ICFW mice inoculated as newborn or adult with F-MuLV and grafted into recipient mice. After repeated in vivo cell transplants, several recombinant polytropic viruses (also termed MCF or dualtropic viruses) were isolated from tumors by limiting dilution....


    pub_type: 杂志文章


    authors: Della-Valle V,Wendling F,Sitbon M,Varlet P,Mathieu-Mahul D,Larsen CJ

    更新日期:1990-10-01 00:00:00

  • Selective integration of avian leukosis virus in different hematopoietic tissues.

    abstract::Hematopoietic tissues obtained from avian leukosis virus (ALV)-infected Hyline SC chickens were analyzed for the presence of integrated viral DNA sequences. Cells were prepared from bone marrow, bursa, spleen, thymus, and peripheral blood. Following the removal of erythrocytes, cellular DNAs from each of these tissues...


    pub_type: 杂志文章


    authors: Baba TW,Humphries EH

    更新日期:1986-12-01 00:00:00

  • Sequestration of human cytomegalovirus by human renal and mammary epithelial cells.

    abstract::Urine and breast milk represent the main routes of human cytomegalovirus (HCMV) transmission but the contribution of renal and mammary epithelial cells to viral excretion remains unclear. We observed that kidney and mammary epithelial cells were permissive to HCMV infection and expressed immediate early, early and lat...


    pub_type: 杂志文章


    authors: Twite N,Andrei G,Kummert C,Donner C,Perez-Morga D,De Vos R,Snoeck R,Marchant A

    更新日期:2014-07-01 00:00:00

  • Evolutionary pathway for the 2017 emergence of a novel highly pathogenic avian influenza A(H7N9) virus among domestic poultry in Tennessee, United States.

    abstract::In March 2017, a novel highly pathogenic avian influenza A(H7N9) virus was detected at two commercial broiler breeder facilities in Tennessee, United States. In this study, a wild bird low pathogenic avian influenza A virus, A/blue-winged teal/Wyoming/AH0099021/2016(H7N9), was shown to be the probable precursor of the...


    pub_type: 杂志文章


    authors: Li L,DeLiberto TJ,Killian ML,Torchetti MK,Wan XF

    更新日期:2018-12-01 00:00:00

  • Human T cell leukemia virus type I Tax activates CD40 gene expression via the NF-kappa B pathway.

    abstract::The human T cell leukemia virus type I (HTLV-I) is an oncogenic retrovirus that is etiologically linked to the genesis of adult T cell leukemia (ATL) as well as HTLV-I-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Emerging evidence suggests that the pathogenicity of HTLV-I involves deregulated activati...


    pub_type: 杂志文章


    authors: Harhaj EW,Harhaj NS,Grant C,Mostoller K,Alefantis T,Sun SC,Wigdahl B

    更新日期:2005-03-01 00:00:00

  • Structural basis for the dimerization and substrate recognition specificity of porcine epidemic diarrhea virus 3C-like protease.

    abstract::Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, has caused significant damage to the Asian and American pork industries. Coronavirus 3C-like protease (3CL(pro)), which is involved in the processing of viral polyproteins for viral replication, is an appealing antiviral drug target. Here,...


    pub_type: 杂志文章


    authors: Ye G,Deng F,Shen Z,Luo R,Zhao L,Xiao S,Fu ZF,Peng G

    更新日期:2016-07-01 00:00:00