Abstract:
:MuSV349 is a TB-cell line which produces infectious MuSV with little or no MuLV detectable by the XC assay. The apparent restriction of MuLV replication in MuSV349 cells was investigated. A replication-competent helper virus, with protein composition nearly identical to that of Mo-MuLV was isolated from the viruses produced by MuSV349 cells. This helper MuLV after it was separated from MuSV and upon infection of TB cells produced viral titer similar to that of Mo-MuLV-infected TB cells indicating that its replication might have been restricted in the MuSV349 cells. To find out whether the suppression of the helper virus replication is due to the genetic peculiarities of the virus or MuSV349 cells, the relative amounts of MuLV and MuSV produced by several distinct clonal MuSV isolates (derived from a common progenitor) upon superinfection with Mo-MuLV were determined. The results of these experiments showed that while both SV7 and SV15F on coinfection with Mo-MuLV produced MuSV in excess over MuLV; and ts110 and tsSV13 on coinfection with Mo-MuLV produced MuLV in excess over MuSV. Since the same Mo-MuLV is used in these experiments and since upon transfer to a different cell, SV7, SV15F, and ts110 retain the property to restrict or not restrict MuLV replication it appears that the above property is determined by the genetics of the MuSV.
journal_name
Virologyjournal_title
Virologyauthors
Yuen PH,Soong MM,Kissil MS,Wong PKdoi
10.1016/0042-6822(84)90043-6subject
Has Abstractpub_date
1984-01-30 00:00:00pages
377-89issue
2eissn
0042-6822issn
1096-0341journal_volume
132pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The PBj14 isolate of simian immunodeficiency virus, SIVsmmPBj14, is an acutely pathogenic lentivirus that causes severe gastrointestinal disease in macaque monkeys. The studies reported here examine the basis for the enteropathic phenotype of SIVsmmPBj14, using flow cytometric analysis of cultured macaque lymphocytes ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0010
更新日期:1996-01-01 00:00:00
abstract::Ten mutations were introduced into the JC virus (JCV) T antigen within a region corresponding to the SV40 T-antigen DNA binding domain (SV40 amino acids 131 to 220); nine of these increased homology between the two proteins in sequences critical for SV40 T antigen DNA binding. All mutant JCV T antigens bound to JCV an...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90136-y
更新日期:1991-07-01 00:00:00
abstract::Cells infected with a reassortant (113/Ho, H7N2) between A/fowl plague/Rostock/34 (FPV, H7N1) and A/Hong Kong/1/68 (H3N2) carrying RNA segments 1 and 6 of the Hong Kong virus and the residual genes of FPV, synthesized at 40 degrees a neuraminidase (NA) which is enzymatically not active and which is not incorporated in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90266-7
更新日期:1986-04-15 00:00:00
abstract::A non-plaque-forming vaccine strain of NDV (NDV-B1) interferes with the infection of cultured chick embryo cells by the virulent plaque-forming NDV-L strain. The level of interference increases when the concentration of interfering virus is increased or when the time between infection and superinfection is increased. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(67)90059-1
更新日期:1967-12-01 00:00:00
abstract::From two human brain tumors SV40 genomes were isolated by recombinant DNA techniques. The SV40 genome cloned from a human meningioma DNA was shown to be indistinguishable from wild-type SV40. In contrast, the SV40 genome cloned from a human astrocytoma proved to be a nonviable deletion mutant with a truncated early re...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90357-x
更新日期:1984-10-30 00:00:00
abstract::The subgroup C human adenovirus L4 33-kDa protein is a nuclear phosphoprotein that plays a direct, but dispensable, role in virion assembly. The r-strand open reading frame (ORF) for this protein lies opposite to the 5' end of the l-strand E2 early (E2E) transcription units. To facilitate studies of regulation of E2E ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1130
更新日期:2001-10-25 00:00:00
abstract::The coat protein (CP) of alfalfa mosaic virus (AMV) strain 425 assembles to bacilliform or rod-shaped particles in the presence of nucleic acids or to T = 1 empty icosahedral particles in the absence of nucleic acids. To study the determinants of CP assembly, recombinant CPs (rCPs) that contained a (His)(6) region wer...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9805
更新日期:1999-07-20 00:00:00
abstract::The nucleotide sequences of the long terminal repeat (LTR) of simian retroviruses (STLV), which are closely related to human T-cell leukemia virus type I (HTLV-I) were found to be of at least two subgroups: an Asian subtype in macaques and an African subtype in African green monkeys and chimpanzee. The nucleotide sequ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90336-3
更新日期:1986-01-30 00:00:00
abstract::Porcine epidemic diarrhea virus (PEDV), a member of the genus Alphacoronavirus, has caused significant damage to the Asian and American pork industries. Coronavirus 3C-like protease (3CL(pro)), which is involved in the processing of viral polyproteins for viral replication, is an appealing antiviral drug target. Here,...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.04.018
更新日期:2016-07-01 00:00:00
abstract::Wild-type vaccinia virus WR strain forms non-fusogenic (F-) large plaques and is hemagglutinin positive (HA+) under normal conditions of virus infection. We have analyzed a collection of spontaneous, highly attenuated mutants of vaccinia virus isolated from persistently infected Friend erythroleukemia cells (E. Paez, ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1018
更新日期:1994-01-01 00:00:00
abstract::Full-length cDNA clones of RNA 2 of tobacco rattle virus (TRV) strain PLB have been cloned into the transcription vector pPM1. Products of in vitro transcription by Escherichia coli RNA polymerase, either capped or uncapped, were as infectious as native RNA 2 when coinoculated with RNA 1 of TRV strain TCM. At least 70...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90222-5
更新日期:1989-11-01 00:00:00
abstract::The Hepatitis B virus precore protein is processed in the endoplasmic reticulum (ER) into secreted hepatitis B e antigen (HBeAg), which acts as an immune tolerogen to establish chronic infection. Downregulation of secreted HBeAg should improve clinical outcome, as patients who effectively respond to current treatments...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.12.034
更新日期:2011-03-01 00:00:00
abstract::Rabies continues to poses serious threats to the public health in many countries. The development of novel inexpensive, safe and effective vaccines has become a high priority for rabies control worldwide. We previously generated a novel recombinant rabies vaccine by cloning rabies virus glycoprotein into a chimpanzee ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.07.022
更新日期:2019-10-01 00:00:00
abstract::Recently, the product of equine herpesvirus type 1 (EHV-1) ORF1, a homolog to HSV-1 pUL56, was shown to modulate MHC-I expression and innate immunity. Here, we investigated modulation of respiratory epithelial immunity by EHV-1 pUL56 and compared responses to those of PBMCs, which are important target cells that allow...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.05.023
更新日期:2014-09-01 00:00:00
abstract::Tetherin has been defined as a restriction factor of HIV-1 and several other enveloped viruses. However, the significance of tetherin in viral infection remains to be further addressed. Here, we investigated whether tetherin plays a role in HSV-2 infection. Our study revealed that overexpression of tetherin restricted...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.11.005
更新日期:2015-01-15 00:00:00
abstract::We recently developed an in vitro transcapsidation system in which infectivity of single-shelled (ss) rotavirus particles was successfully rescued (Chen and Ramig, Virology [1993]). Here, we report the rescue of infectivity of rotavirus core particles using virus strain B223 (G serotype 10) as the core donor and strai...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1315
更新日期:1993-06-01 00:00:00
abstract::We recently reported the phenomenon of retroviral insertion into a herpesvirus (Isfort et al., (1992) Proc. Natl. Acad. Sci. USA 89, 991-995). We have now isolated Marek's disease virus (MDV) clones that carry retroviral inserts. Retroviral LTR insertion has the potential to activate or inactivate herpesvirus genes le...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1018
更新日期:1993-01-01 00:00:00
abstract::HIV-1 envelope glycoproteins (Env) and Env-based immunogens usually do not interact efficiently with the inferred germline precursors of known broadly neutralizing antibodies (bNAbs). This deficiency may be one reason why Env and Env-based immunogens are not efficient at inducing bNAbs. We evaluated the binding of 15 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.08.002
更新日期:2015-12-01 00:00:00
abstract::Monoclonal antibodies directed against glycoprotein D of herpes simplex virus completely inhibited fusion of Vero cells infected with type 1 virus. In contrast, several monoclonal antibodies directed against other viral glycoproteins, including B, were ineffective or were only minimally inhibitory at the highest conce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90409-9
更新日期:1983-08-01 00:00:00
abstract::To define the number of rhinovirus serotypes, cross neutralization tests and characterization studies were completed on 25 candidate prototype rhinoviruses submitted to a third phase of a collaborative program. Based on the results, 11 distinct prototype strains were designated and the numbering system was extended to...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90367-9
更新日期:1987-07-01 00:00:00
abstract::The octadecyl rhodamine (R18) fluorescent dequenching assay was used to examine membrane fusion between mumps virus and mammalian cells. Rapid fluorescent dequenching, indicative of membrane fusion, was observed when labeled mumps virus was mixed with either ghost erythrocytes or CV-1 cells. After 15 min a saturation ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90196-v
更新日期:1992-11-01 00:00:00
abstract::HIV-1 genomic RNA (gRNA) dimerization is important for viral infectivity and is regulated by proteolytic processing of the Gag precursor protein (Pr55gag) under the direction of the viral protease. The processing occurs in successive steps and, to date, the step associated with formation of a wild-type (WT) level of g...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.11.028
更新日期:2009-03-01 00:00:00
abstract::The sequence of gene VI from figwort mosaic virus (FMV) clone x4 was determined and compared with that previously published for FMV clone DxS. Both clones originated from the same virus isolation, but the virus used to clone DxS was propagated extensively in a host of a different family prior to cloning whereas that u...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90624-k
更新日期:1991-06-01 00:00:00
abstract::Following an increase in detection of enterovirus 68 (EV68) in community surveillance of respiratory infections in The Netherlands in 2010, epidemiological and virological analyses were performed to investigate the possible public health impact of EV68 infections. We retrospectively tested specimens collected from acu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.11.021
更新日期:2012-02-05 00:00:00
abstract::Human adenovirus type 5 (Ad5) has been the most popular platform for the development of oncolytic Ads. Alternative Ad serotypes with low seroprevalence might allow for improved anticancer efficacy in Ad5-immune patients. We studied the safety and efficacy of rare serotypes Ad6, Ad11 and Ad35. In vitro cytotoxicity of ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.08.038
更新日期:2009-11-25 00:00:00
abstract::Most adenovirus-specific mRNAs expressed late after infection originate from a single transcription unit, the so-called major late transcription unit. All mRNAs expressed from this unit have in common a 201 nucleotide-long spliced tripartite leader segment attached to their 5' ends. Human adenovirus mutants that carry...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1234
更新日期:1993-05-01 00:00:00
abstract::We have developed a sensitive assay for DNA replication in mammalian cells that enables us to detect replicated DNA fragments of human cytomegalovirus. A 1/100 portion of DNA extracted from 1000 microinjected cells was subjected to polymerase chain reaction after digestion with appropriate restriction enzymes to diffe...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1038
更新日期:1993-01-01 00:00:00
abstract::Angiotensin converting enzyme 2 (ACE2) is the receptor that severe acute respiratory syndrome coronavirus (SARS-CoV) utilizes for target cell entry and, therefore, plays an important role in SARS pathogenesis. Since Chinese rhesus (rh) macaques do not usually develop SARS after SARS-CoV infection, it has been suggeste...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.08.016
更新日期:2008-11-10 00:00:00
abstract::Enterovirus 71 (EV71) is a major cause of hand-foot-and-mouth disease. EV71 infection occasionally associates with severe neurological sequelae such as brainstem encephalitis or poliovirus-like paralysis. We demonstrated that mouse-adapted strain increases infectivity, resulting in higher cytotoxicity of neuron cells ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.10.015
更新日期:2012-01-05 00:00:00
abstract::Chlorella virus PBCV-1 encodes two putative chitinase genes, a181/182r and a260r, and one chitosanase gene, a292l. The three genes were cloned and expressed in Escherichia coli. The recombinant A181/182R protein has endochitinase activity, recombinant A260R has both endochitinase and exochitinase activities, and recom...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9958
更新日期:1999-10-25 00:00:00