Abstract:
:The octadecyl rhodamine (R18) fluorescent dequenching assay was used to examine membrane fusion between mumps virus and mammalian cells. Rapid fluorescent dequenching, indicative of membrane fusion, was observed when labeled mumps virus was mixed with either ghost erythrocytes or CV-1 cells. After 15 min a saturation limit of 18 virus per erythrocyte ghost and 6400 virus per CV-1 cell was observed. Fetuin was found to inhibit virus fusion, suggesting a role for sialic acid in virus binding to the cells. Two dequenching processes were observed of which the faster process is thought to be membrane fusion and the second process is thought to be probe proximal transfer.
journal_name
Virologyjournal_title
Virologyauthors
Di Simone C,Baldeschwieler JDdoi
10.1016/0042-6822(92)90196-vsubject
Has Abstractpub_date
1992-11-01 00:00:00pages
338-45issue
1eissn
0042-6822issn
1096-0341journal_volume
191pub_type
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