Membrane fusion of mumps virus with ghost erythrocytes and CV-1 cells.

Abstract:

:The octadecyl rhodamine (R18) fluorescent dequenching assay was used to examine membrane fusion between mumps virus and mammalian cells. Rapid fluorescent dequenching, indicative of membrane fusion, was observed when labeled mumps virus was mixed with either ghost erythrocytes or CV-1 cells. After 15 min a saturation limit of 18 virus per erythrocyte ghost and 6400 virus per CV-1 cell was observed. Fetuin was found to inhibit virus fusion, suggesting a role for sialic acid in virus binding to the cells. Two dequenching processes were observed of which the faster process is thought to be membrane fusion and the second process is thought to be probe proximal transfer.

journal_name

Virology

journal_title

Virology

authors

Di Simone C,Baldeschwieler JD

doi

10.1016/0042-6822(92)90196-v

subject

Has Abstract

pub_date

1992-11-01 00:00:00

pages

338-45

issue

1

eissn

0042-6822

issn

1096-0341

journal_volume

191

pub_type

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