Hepatitis C virus RNA polymerase and NS5A complex with a SNARE-like protein.

Abstract:

:Hepatitis C virus (HCV) NS5A is a phosphoprotein that possesses a cryptic trans-activation activity. To investigate its potential role in viral replication, we searched for the cellular proteins interacting with NS5A protein by yeast two-hybrid screening of a human hepatocyte cDNA library. We identified a newly discovered soluble N-ethylmaleimide-sensitive factor attachment protein receptor-like protein termed human vesicle-associated membrane protein-associated protein of 33 kDa (hVAP-33). In vitro binding assay and in vivo coimmunoprecipitation studies confirmed the interaction between hVAP-33 and NS5A. Interestingly, hVAP-33 was also shown to interact with NS5B, the viral RNA-dependent RNA polymerase. NS5A and NS5B bind to different domains of hVAP-33: NS5A binds to the C-terminus, whereas NS5B binds to the N-terminus of hVAP-33. Immunofluorescent staining showed a significant colocalization of hVAP-33 with both NS5A and NS5B proteins. hVAP-33 contains a coiled-coil domain followed by a membrane-spanning domain at its C-terminus. Cell fractionation analysis revealed that hVAP-33 is predominantly associated with the ER, the Golgi complex, and the prelysosomal membrane, consistent with its potential role in intracellular membrane trafficking. These interactions provide a mechanism for membrane association of the HCV RNA replication complex and further suggest that NS5A is a part of the viral RNA replication complex.

journal_name

Virology

journal_title

Virology

authors

Tu H,Gao L,Shi ST,Taylor DR,Yang T,Mircheff AK,Wen Y,Gorbalenya AE,Hwang SB,Lai MM

doi

10.1006/viro.1999.9893

subject

Has Abstract

pub_date

1999-10-10 00:00:00

pages

30-41

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(99)99893-8

journal_volume

263

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • The flavivirus 3'-noncoding region: extensive size heterogeneity independent of evolutionary relationships among strains of tick-borne encephalitis virus.

    abstract::The sequences of the 3'-noncoding regions (NCR) of 12 strains of tick-borne encephalitis (TBE) virus were analyzed and found to vary in length from 350 to approximately 750 nucleotides. The size heterogeneity is restricted to a variable region following the stop codon, whereas the most 3'-terminal 350 nucleotides form...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1995.1557

    authors: Wallner G,Mandl CW,Kunz C,Heinz FX

    更新日期:1995-10-20 00:00:00

  • Mapping of 5' ends of virion-derived HBV DNA.

    abstract::Mapping of the 5' ends of virion-derived hepatitis B virus DNA molecules was carried out after cloning and sequencing linearized genomes made fully double stranded. Of five clones obtained, three of four minus strand termini mapped to the G in position 1826 and one to the T in position 1827. Plus strand 5' ends were m...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(92)90765-h

    authors: Saldanha JA,Qiu H,Thomas HC,Monjardino J

    更新日期:1992-05-01 00:00:00

  • Sequence diversity of the capsid gene and the nonstructural gene NS2B of dengue-3 virus in vivo.

    abstract::Previously, we studied the envelope (E) gene of dengue virus and reported that dengue-3 virus is present as a quasispecies. To investigate the extent of intrahost sequence variation of other dengue viral genes, we examined in this study the capsid (C) gene and the nonstructural gene, NS2B, derived directly from plasma...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.2002.1635

    authors: Wang WK,Sung TL,Lee CN,Lin TY,King CC

    更新日期:2002-11-10 00:00:00

  • The herpes simplex virus immediate-early protein ICP27 shuttles between nucleus and cytoplasm.

    abstract::ICP27 is an essential herpes simplex virus type 1 (HSV-1) nuclear protein which regulates viral early and late genes during infection. The exact mechanism by which ICP27 modulates viral gene expression is unknown, but considerable evidence suggests that it functions posttranscriptionally. In this study, we have asked ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1997.9006

    authors: Mears WE,Rice SA

    更新日期:1998-03-01 00:00:00

  • Amino acid sequence analysis of bovine rotavirus B223 reveals a unique outer capsid protein VP4 and confirms a third bovine VP4 type.

    abstract::The nucleotide and deduced amino acid sequence of the gene 4 of bovine rotavirus strain B223 is described. The open reading frame is predicted to encode a VP4 of 772 amino acids, shorter than described for any other rotavirus strain sequenced to date. B223 VP4 shows 70 to 73% similarity to other rotavirus VP4 proteins...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(92)90191-q

    authors: Hardy ME,Gorziglia M,Woode GN

    更新日期:1992-11-01 00:00:00

  • The complete genome structure and synthesis of infectious RNA from clones of tomato bushy stunt virus.

    abstract::The complete sequence of the genome of the cherry strain of tomato bushy stunt virus (TBSV), a member of the tombusvirus group, was determined. A full-length clone of the genome containing a bacteriophage T7 RNA polymerase promoter was assembled from partial cDNA clones. In vitro transcripts of the genome, either with...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(90)90468-7

    authors: Hearne PQ,Knorr DA,Hillman BI,Morris TJ

    更新日期:1990-07-01 00:00:00

  • Characterization of proteinase cleavage sites in the N-terminal region of the RNA1-encoded polyprotein from Arabis mosaic virus (subgroup A nepovirus).

    abstract::Arabis mosaic virus is a subgroup A nepovirus. The RNA1-encoded polyprotein (P1) contains the domains for the NTP-binding protein (NTB), VPg, proteinase (Pro) and polymerase at its C-terminus. Putative cleavage sites delineating these domains have been proposed. However, the number and location of cleavage sites upstr...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2008.01.046

    authors: Wetzel T,Chisholm J,Bassler A,Sanfaçon H

    更新日期:2008-05-25 00:00:00

  • Insights into the interaction between tRNA and primer binding site from characterization of a unique HIV-1 virus which stably maintains dual PBS complementary to tRNA(Gly) and tRNA(His).

    abstract::Previously our laboratory constructed an HIV-1 which stably maintained a primer binding site (PBS) complementary to tRNA(His) by mutating the region of the provirus within U5 postulated to interact with the anticodon of tRNA(His) (J. Wakefield, S-M Kang, and C. D. Morrow, 1996, J. Virol, 70, 966-975). From the analysi...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1997.8837

    authors: Li Y,Zhang Z,Kang SM,Buescher JL,Morrow CD

    更新日期:1997-11-24 00:00:00

  • Cellular Hsp27 interacts with classical swine fever virus NS5A protein and negatively regulates viral replication by the NF-κB signaling pathway.

    abstract::Classical swine fever virus (CSFV) nonstructural protein NS5A is a multifunctional protein functioning in regulation of viral genome replication, protein translation and assembly by interaction with viral or host proteins. Here, heat shock protein 27 (Hsp27) has been identified as a novel binding partner of NS5A by us...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2018.02.020

    authors: Ling S,Luo M,Jiang S,Liu J,Ding C,Zhang Q,Guo H,Gong W,Tu C,Sun J

    更新日期:2018-05-01 00:00:00

  • Abolition of hCAR-dependent cell tropism using fiber knobs of Atadenovirus serotypes.

    abstract::Most adenoviral vectors use in gene therapy protocols derive from species C. However, expression of the primary receptor (human Coxsackievirus and Adenovirus receptor, hCAR) for these AdV is variable on cancer cells. In vivo targeting of a therapeutic gene to specific cells has then become a major issue in gene therap...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2003.12.023

    authors: Renaut L,Colin M,Leite JP,Benko M,D'Halluin JC

    更新日期:2004-04-10 00:00:00

  • The role of Th17 cells and regulatory T cells in Coxsackievirus B3-induced myocarditis.

    abstract::IL-17-producing (Th17) and regulatory T (Treg) cells have been well established in the pathogenesis of many inflammatory diseases. To assess whether Th17 and Treg were altered in acute virus-induced myocarditis (AVMC) mice, we assessed Th17/Treg functions on different levels in AVMC. It was shown that the expression o...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2011.09.006

    authors: Xie Y,Chen R,Zhang X,Chen P,Liu X,Xie Y,Yu Y,Yang Y,Zou Y,Ge J,Chen H

    更新日期:2011-12-05 00:00:00

  • Tyrosine sulfation of varicella-zoster virus envelope glycoprotein gpl.

    abstract::Sulfation is a common post-translational modification of secreted and membrane proteins, with the sulfate attached to tyrosine residues or to glycan side-chains. I have shown that varicella-zoster virus (VZV) envelope glycoproteins gpI, gpII, and gpIII can be labeled with [35S]sulfate. The predominant VZV glycoprotein...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1993.1576

    authors: Edson CM

    更新日期:1993-11-01 00:00:00

  • The outer capsid protein VP4 of equine rotavirus strain H-2 represents a unique VP4 type by amino acid sequence analysis.

    abstract::The nucleotide and deduced amino acid sequence of G serotype 3 equine rotavirus strain H-2 was determined. A predicted 776-amino-acid H-2 VP4 shows less than or equal to 85.3% identity to other rotavirus VP4 types sequenced to date and thus represents a new P serotype. A PCR-generated probe derived from a cDNA clone o...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1993.1152

    authors: Hardy ME,Gorziglia M,Woode GN

    更新日期:1993-03-01 00:00:00

  • Use of a high-affinity peptide that aborts MHC-restricted cytotoxic T lymphocyte activity against multiple viruses in vitro and virus-induced immunopathologic disease in vivo.

    abstract::Binding of a specific peptide(s) from a viral protein to major histocompatibility complex (MHC) class I molecules is a critical step in the activation of CD8(+) cytotoxic T lymphocytes (CTLs). Once activated, CTLs can cause lethal disease in an infected host, for example, by killing virus-containing ependymal and vent...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1998.9593

    authors: Oldstone MB,von Herrath M,Lewicki H,Hudrisier D,Whitton JL,Gairin JE

    更新日期:1999-04-10 00:00:00

  • Identification of a new neutralization epitope on VP7 of human serotype 2 rotavirus and evidence for electropherotype differences caused by single nucleotide substitutions.

    abstract::Neutralizing monoclonal antibodies 2F1 and 1C10, which are specific for VP7 of serotype 2 rotaviruses (G2), were used to select neutralization escape variants of the human serotype 2 rotavirus, DS-1. Neutralization survival patterns generated by monoclonal antibodies 2F1, 1C10, and RV5:3 indicated that 2F1 and 1C10 di...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1993.1601

    authors: Dunn SJ,Ward RL,McNeal MM,Cross TL,Greenberg HB

    更新日期:1993-11-01 00:00:00

  • Are the acid-labile interferon alpha and interferon omega-1 identical?

    abstract::The interferon (IFN) activity found in human leukocyte IFN alpha preparations, autoimmune and AIDS sera, and others was reported to have distinct antigenic and deviating biological properties. This led to its vague designation as acid-labile and thermolabile IFN alpha. However, using specific monoclonal antibodies, th...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(91)90517-f

    authors: Kontsek P,Borecký L,Novák M

    更新日期:1991-03-01 00:00:00

  • Delineation of a 16 amino acid sequence that forms a core DNA recognition motif in the Epstein-Barr virus EBNA-1 protein.

    abstract::EBNA-1 is the sole virally specified protein required for replication of the Epstein-Barr virus latency origin and also modulates its own expression. Both of these functions are mediated through specific DNA binding. We created a series of amino acid substitutions across the previously identified DNA-binding domain of...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1994.1669

    authors: Chen MR,Zong J,Hayward SD

    更新日期:1994-12-01 00:00:00

  • Strains and mutants of tobacco mosaic virus are both found in virus derived from single-lesion-passaged inoculum.

    abstract::An analysis has been made of variants found in preparations of the common (U1) strain of tobacco mosaic virus (TMV) which had been obtained after the inoculum had been passaged through a necrotic local lesion. These lesions were induced with a minimal number of virions (3000 or 30,000) per inoculation point. The relat...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(84)90097-7

    authors: García-Arenal F,Palukaitis P,Zaitlin M

    更新日期:1984-01-15 00:00:00

  • Primary HIV-1 R5 isolates from end-stage disease display enhanced viral fitness in parallel with increased gp120 net charge.

    abstract::To better understand the evolution of the viral envelope glycoproteins (Env) in HIV-1 infected individuals who progress to AIDS maintaining an exclusive CCR5-using (R5) virus population, we cloned and sequenced the env gene of longitudinally obtained primary isolates. A shift in the electrostatic potential towards an ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2008.06.014

    authors: Repits J,Sterjovski J,Badia-Martinez D,Mild M,Gray L,Churchill MJ,Purcell DF,Karlsson A,Albert J,Fenyö EM,Achour A,Gorry PR,Jansson M

    更新日期:2008-09-15 00:00:00

  • A varicella-zoster virus mutant impaired for latency in rodents, but not impaired for replication in cell culture.

    abstract::While trying to generate a site-directed deletion in the ORF63 latency-associated gene of varicella-zoster virus (VZV) Oka, we constructed a virus with an unexpected rearrangement. The virus has a small deletion in both copies of ORF63 and two copies of a cassette inserted between ORFs 64/65 and 68/69 containing (a) t...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2010.01.007

    authors: Ambagala AP,Krogmann T,Qin J,Pesnicak L,Cohen JI

    更新日期:2010-04-10 00:00:00

  • Characterization of the polypeptides and determination of genome coding assignments of an aquareovirus.

    abstract::Proteins of an aquareovirus isolated from striped bass (SBR strain) were examined. Twelve viral-specific proteins ranging in molecular weight from approximately 130 to 15 kDa were identified in infected cells. Purified virions and infected cell lysates were used to characterize the structural and nonstructural protein...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1994.1621

    authors: Subramanian K,McPhillips TH,Samal SK

    更新日期:1994-11-15 00:00:00

  • Cell surface CD4 downregulation and resistance to superinfection induced by a defective provirus of HIV-1.

    abstract::Proviral sequences encoding defective HIV-1 regulatory genes have been detected previously in infected individuals; however, the role of these defective genomes in pathogenesis is unclear. The hypothesis that such replication-defective genomes might induce downregulation of the cellular receptor for HIV-1 (CD4) was te...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1994.1683

    authors: Little SJ,Riggs NL,Chowers MY,Fitch NJ,Richman DD,Spina CA,Guatelli JC

    更新日期:1994-12-01 00:00:00

  • The capsid architecture of channel catfish virus, an evolutionarily distant herpesvirus, is largely conserved in the absence of discernible sequence homology with herpes simplex virus.

    abstract::Although herpesviruses have a wide host range and their genomes vary substantially in size, the nucleocapsid appears to be a conservative element of viral design. The capsid shell is icosahedrally symmetric (T = 16), and 125 nm in diameter and 15nm thick in the case of herpes simplex virus 1 (HSV-1). Channel catfish v...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1996.0016

    authors: Booy FP,Trus BL,Davison AJ,Steven AC

    更新日期:1996-01-15 00:00:00

  • vFLIP upregulates IKKε, leading to spindle morphology formation through RelA activation.

    abstract::Kaposi's sarcoma (KS)-associated herpesvirus (KSHV) vFLIP, a latent gene of KSHV, was first identified as a FLICE-inhibitory protein (FLIP) protecting cells from apoptosis. The vFLIP protein has been shown to activate the NF-κB signaling involved in spindle morphology formation both in HUVECs infected with KSHV and Ka...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2018.07.007

    authors: Yang Z,Honda T,Ueda K

    更新日期:2018-09-01 00:00:00

  • Specific restrictions in the progression of Venezuelan equine encephalitis virus-induced disease resulting from single amino acid changes in the glycoproteins.

    abstract::The pathogenesis of Venezuelan equine encephalitis virus (VEE) was examined in the mouse model using V3000, a virus derived from a molecular clone of the Trinidad donkey strain of VEE. These results were compared in parallel experiments with avirulent mutants of VEE derived by site-directed mutagenesis of the clone. A...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1995.1022

    authors: Grieder FB,Davis NL,Aronson JF,Charles PC,Sellon DC,Suzuki K,Johnston RE

    更新日期:1995-02-01 00:00:00

  • Enhanced production of morbillivirus gene-specific RNAs following induction of the cellular stress response in stable persistent infection.

    abstract::Previous in vitro work demonstrated the incorporation of the major inducible 70k heat shock protein (i.e., 72k HSP) into the biologically active light nucleocapsid (L-NC) variant of canine distemper virus (CDV). Here, in vitro induction of the cellular stress response, characterized by elevated cytoplasmic and intranu...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1993.1072

    authors: Oglesbee MJ,Kenney H,Kenney T,Krakowka S

    更新日期:1993-02-01 00:00:00

  • Complementation of enteric adenovirus type 40 for lytic growth in tissue culture by E1B 55K function of adenovirus types 5 and 12.

    abstract::The enteric adenovirus type 40 strain Dugan (Ad40) cannot be passaged in HeLa cells, but will grow in 293 cells, which express Ad5 E1 functions. To determine the reason for this limited host range, KB cell lines expressing Ad2 E1A, E1B, or E1A + E1B (L. E. Babiss, C. S. H. Young, P. B. Fisher, and H. S. Ginsberg, 1983...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(89)90634-x

    authors: Mautner V,Mackay N,Steinthorsdottir V

    更新日期:1989-08-01 00:00:00

  • Identification and characterization of a murine cytomegalovirus gene with homology to the UL25 open reading frame of human cytomegalovirus.

    abstract::Monoclonal antibody 1B4, previously shown to be protective in vivo and to cross-react with both virally encoded and normal host cell proteins, was used to screen a lambda gt11 cDNA derived from mRNA harvested from mouse embryo fibroblasts 24 hr after infection with murine cytomegalovirus (MCMV). A 700-bp cDNA was iden...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1994.1227

    authors: Dallas PB,Lyons PA,Hudson JB,Scalzo AA,Shellam GR

    更新日期:1994-05-01 00:00:00

  • Gammaherpesvirus targets peritoneal B-1 B cells for long-term latency.

    abstract::Gammaherpesviruses establish life-long infection in most adults and are associated with the development of B cell lymphomas. While the interaction between gammaherpesviruses and splenic B cells has been explored, very little is known about gammaherpesvirus infection of B-1 B cells, innate-like B cells that primarily r...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2016.02.022

    authors: Rekow MM,Darrah EJ,Mboko WP,Lange PT,Tarakanova VL

    更新日期:2016-05-01 00:00:00

  • Protection from pathogenic SIVmac challenge following short-term infection with a nef-deficient attenuated virus.

    abstract::Infection of rhesus macaques with attenuated SIVmac is, at present, the only strategy which confers significant protection from challenge with wild-type SIVmac grown in monkey PBMC. However, initial results suggest that the protective mechanism does not develop until late after "vaccination" (approx 10 months). As par...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1996.0237

    authors: Norley S,Beer B,Binninger-Schinzel D,Cosma C,Kurth R

    更新日期:1996-05-01 00:00:00