Abstract:
:Evidence is presented for the glycosylation of the cauliflower mosaic virus (CaMV) capsid polypeptide. Fluorescent derivatives of concanavalin A (Con A) were shown to bind specifically to the dissociated CaMV capsid polypeptides in polyacrylamide gels. Con A also bound specifically to intact CaMV, so inhibiting the attachment of virus-specific antibodies. The role of carbohydrate residues in CaMV antigenicity is evaluated.
journal_name
Virologyjournal_title
Virologyauthors
Du Plessis DH,Smith Pdoi
10.1016/0042-6822(81)90510-9subject
Has Abstractpub_date
1981-03-01 00:00:00pages
403-8issue
2eissn
0042-6822issn
1096-0341pii
0042-6822(81)90510-9journal_volume
109pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The monoclonal antibody 2D5 neutralized vaccinia virus by preventing penetration of the virus and reacting with VP23-29K. The conformation of the VP23-29K was maintained by a disulfide bond(s), and the 2D5mAb reacted stronger with the nonreduced 23-kDa form than with the reduced 29-kDa form. We selected several escape...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0337
更新日期:1996-06-15 00:00:00
abstract::The spike glycoprotein (G protein) of rabies virus (CVS strain) expressed in HeLa cells from cloned cDNA mediated membrane fusion after exposure to pHs of 6.1 or below. Chemical crosslinking showed that the rabies G protein, like the vesicular stomatitis virus (VSV) G protein, could be crosslinked to dimers and trimer...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90539-n
更新日期:1991-12-01 00:00:00
abstract::The single-cell ELISPOT assay was used to determine the frequency and isotype commitment of virus-specific antibody-forming cells (AFC) at different anatomical locations following intranasal Sendai virus infection of C57BL/6 and 129/Sv mice. AFC responses in the mediastinal and cervical lymph nodes showed sharp increa...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1079
更新日期:1995-02-20 00:00:00
abstract::We describe the in vitro selection and characterisation of virus derived from B/Beijing/1/87 passaged in the presence of zanamivir. During zanamivir passage, the phenotype of virus isolates was either drug dependent or drug resistant in plaque reduction assays. The susceptibility of the neuraminidase of the drug-depen...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0058
更新日期:1999-12-20 00:00:00
abstract::Human infections with highly pathogenic H5N1 avian influenza viruses continue to occur in many parts of the world and pose a considerable public health threat. With the use of animal models, the identification of virulence determinants has been instrumental in improving our understanding of how these viruses cause sev...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.02.005
更新日期:2011-05-10 00:00:00
abstract::Naturally occurring mutations in Human T-cell Leukemia Virus Type 1 (HTLV-1) Tax protein lead to loss of recognition by cytotoxic T-lymphocytes. Most of these mutations also abolish or severely impair the transactivation function of Tax. Ninety percent of the rex gene, which encodes the viral regulator of mRNA splicin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8789
更新日期:1997-10-27 00:00:00
abstract::In order to investigate the global and dynamic host microRNAs (miRNAs)/messenger RNAs (mRNAs) expression alteration during in vitro acute HCV infection, a comprehensive microarray analysis was performed using human hepatoma cells. Totally, 108 human miRNAs and 1247 mRNAs were identified whose expression levels changed...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.11.036
更新日期:2010-03-01 00:00:00
abstract::An infectious raccoon poxvirus (RCNV) was used to express the feline panleukopenia virus (FPV) open reading frame VP2. The recombinant, RCNV/FPV, was constructed by homologous recombination with a chimeric plasmid for inserting the expression cassette into the thymidine kinase (TK) locus of RCNV. Expression of the VP2...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0186
更新日期:1996-04-01 00:00:00
abstract::We have previously described a selective increase in HIV-DNA content in CCR5-negative lymphocytes from late stage HIV-infected patients. Here, we show that this increase occurred even in the absence of viral phenotypic switching from CCR5- to CXCR4-tropic. This leads us to hypothesize that early and late CCR5-tropic v...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.12.003
更新日期:2006-04-10 00:00:00
abstract::A resonance energy transfer assay of membrane fusion developed by P. S. Uster and D. W. Deamer (Biochemistry 24, 1-8 (1985)) was used in a study of influenza haemagglutinin-mediated fusion. The characteristics of fusion and haemolysis by X-31 (H3N2) virus, a number of mutants of X-31 which fuse membranes at higher pH,...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90083-8
更新日期:1986-02-01 00:00:00
abstract::Interleukin 2-inducible T cell kinase (ITK) influences T cell signaling by coordinating actin polymerization and polarization as well as recruitment of kinases and adapter proteins. ITK regulates multiple steps of HIV-1 replication, including virion assembly and release. Fluorescent microscopy was used to examine the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.11.015
更新日期:2013-02-05 00:00:00
abstract::The minimal 5' initiation of assembly sequence for papaya mosaic virus (PMV) has been determined by the generation of successive 3' deletion clones from a full-length cDNA copy of the PMV genome followed by in vitro assembly of RNA transcripts. Sequences closest to the 5' terminus were cloned into plasmid vectors with...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1118
更新日期:1994-02-15 00:00:00
abstract::This report presents a procedure for evaluating the relative affinities of simple sialoside receptor determinants for their interaction with the receptor binding pocket of the influenza virus hemagglutinin. The virus examined was A/Memphis/102/72, previously shown to exhibit preferential binding specificity for enzyma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90026-2
更新日期:1987-10-01 00:00:00
abstract::Hematopoietic tissues obtained from avian leukosis virus (ALV)-infected Hyline SC chickens were analyzed for the presence of integrated viral DNA sequences. Cells were prepared from bone marrow, bursa, spleen, thymus, and peripheral blood. Following the removal of erythrocytes, cellular DNAs from each of these tissues...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90216-3
更新日期:1986-12-01 00:00:00
abstract::The N-terminal portion of the reovirus cell attachment protein sigma 1 has recently been shown to possess intrinsic oligomerization and virion-anchoring functions. Sequence analysis of the sigma 1 proteins of the three reovirus serotypes has revealed the presence of distinct structural domains within this region: a te...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90678-5
更新日期:1991-05-01 00:00:00
abstract::Herpes simplex viruses encode several glycoproteins dispensable for infection and replication in cell culture. Evidence is presented that there exist at least two pathways for viral attachment to cells, i.e., one mediated by the dispensable glycoprotein C (gC) and one independent of that glycoprotein. Thus, whereas th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90396-9
更新日期:1990-09-01 00:00:00
abstract::The complete nucleotide sequence of the middle component RNA (M RNA) of the comovirus bean pod mottle virus (BPMV) has been determined. The sequence consists of 3662 nucleotides and contains a single long open reading frame sufficient to code for a protein of 113,353 Da. The proteolytic processing sites within this pr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90155-5
更新日期:1991-07-01 00:00:00
abstract::Human immunodeficiency virus type 1 subtype C (HIV-1C) accounts for about 50% of all HIV infections in the pandemic and is the predominant subtype in the heavily burdened region of southern Africa. HIV-1C possesses unique genetic and phenotypic features that might be associated with biological differences compared to ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.11.047
更新日期:2006-04-10 00:00:00
abstract::We compared the in vitro fidelity of wild-type human immunodeficiency virus type-1 (HIV-1) reverse transcriptase (RT) and the prototype foamy virus (PFV) RT. Both enzymes had similar error rates for single nucleotide substitutions; however, PFV RT did not appear to make errors at specific hotspots, like HIV-1 RT. In a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.05.034
更新日期:2007-10-25 00:00:00
abstract::The early steps of Rous sarcoma virus-Bryan strain (BS-RSV) infection of genetically resistant and susceptible chick embryo cells were investigated. The methods used were the uptake of virus by cells grown in monolayer, and the ability of infected cells to form foci of transformed cells after plating on resistant and ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(67)90046-3
更新日期:1967-08-01 00:00:00
abstract::A recombinant vaccinia virus that expresses the nucleoprotein gene of Lassa virus (Josiah strain) under the control of the P7.5 promoter was constructed using the lacZ coexpression transfer vector pSC11. Southern blot analysis demonstrated that recombination of the sequences inserted within the thymidine kinase gene o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90525-4
更新日期:1989-07-01 00:00:00
abstract::The primer binding site (PBS) plays pivotal roles during reverse transcription of retroviruses and also is the target of a cellular host defense impeding the transcription of murine leukemia virus (MLV) harboring a proline (pro) PBS in embryonic cells. Both the PBS and the tRNA primer are copied during reverse transcr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0683
更新日期:2000-12-20 00:00:00
abstract::Open reading frame 89 (ORF89) is one of the three genes that are believed to be involved in the latent infection of white spot syndrome virus (WSSV). Here, we report the structure and functional characterization of ORF89. cDNA sequencing, 5' RLM-RACE, and 3' RLM-RACE showed that ORF89 gene is transcribed into an unspl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.05.005
更新日期:2004-07-20 00:00:00
abstract::Viruses in certain genera in family Caulimoviridae were shown to integrate their genomic sequences into their host genomes and exist as endogenous pararetroviral sequences (EPRV). However, members of the genus Caulimovirus remained to be the exception and are known to exist only as episomal elements in the infected ce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.03.003
更新日期:2008-07-05 00:00:00
abstract::The specificity of poliovirus encapsidation has been studied using a novel chimeric genome in which the gene encoding firefly luciferase has been substituted for the VP2-VP3-VP1 genes of the poliovirus capsid (P1) gene. Transfection of RNA transcribed in vitro from this genome resulted in a VP4-luciferase fusion prote...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9046
更新日期:1998-03-30 00:00:00
abstract::In HIV-1 infection, plasma viral load (VL) has dual implications for pathogenesis and public health. Based on well-known patterns of HIV-1 evolution and immune escape, we hypothesized that VL is an evolving quantitative trait that depends heavily on duration of infection (DOI), demographic features, human leukocyte an...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.11.024
更新日期:2014-01-20 00:00:00
abstract::Prostaglandin E₂ (PGE₂) is an arachidonic acid (AA)-derived signaling molecule that can influence host immune responses to infection or vaccination. In this study, we investigated PGE₂ production in vitro by cells infected with the poxvirus vaccine strain, modified vaccinia Ankara virus (MVA). Human THP-1 cells, murin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.03.019
更新日期:2012-07-05 00:00:00
abstract::The hepatitis B virus (HBV) is formed by budding. A stretch of 22 amino acids (aa) (matrix domain, MD, R103 - S124) in the large envelope protein L is crucial for virion formation and probably establishes contact to the nucleocapsid. Here, we assess the impact of sequence variations at numerous individual aa positions...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.04.030
更新日期:2014-06-01 00:00:00
abstract::Bombyx mori nucleopolyhedrovirus (BmNPV) orf11 (bm11) is a highly conserved gene with unknown function. It is homologous to AcMNPV orf19. In this study, a bm11 knockout virus was constructed and its role was investigated. Expression analysis indicated that bm11 is a late gene and confocal microscopy analysis demonstra...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.10.026
更新日期:2019-01-15 00:00:00
abstract::While several studies have shown a role for autophagy in the replication of dengue virus (DENV), these studies have been performed in directly infected cells. However, in severe cases of DENV infection the critical cell in the disease is believed to be monocytes which are poorly infected directly, but are highly susce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.07.010
更新日期:2011-09-15 00:00:00