Abstract:
:The recently described taxon Negevirus is comprised of a diverse group of insect-specific viruses isolated from mosquitoes and phlebotomine sandflies. In this study, a comprehensive genetic characterization, molecular, epidemiological and evolutionary analyses were conducted on nearly full-length sequences of 91 new negevirus isolates obtained in Brazil, Colombia, Peru, Panama, USA and Nepal. We demonstrated that these arthropod restricted viruses are clustered in two major phylogenetic groups with origins related to three plant virus genera (Cilevirus, Higrevirus and Blunevirus). Molecular analyses demonstrated that specific host correlations are not present with most negeviruses; instead, high genetic variability, wide host-range, and cross-species transmission were noted. The data presented here also revealed the existence of five novel insect-specific viruses falling into two arthropod-restrictive virus taxa, previously proposed as distinct genera, designated Nelorpivirus and Sandewavirus. Our results provide a better understanding of the molecular epidemiology, evolution, taxonomy and stability of this group of insect-restricted viruses.
journal_name
Virologyjournal_title
Virologyauthors
Nunes MRT,Contreras-Gutierrez MA,Guzman H,Martins LC,Barbirato MF,Savit C,Balta V,Uribe S,Vivero R,Suaza JD,Oliveira H,Nunes Neto JP,Carvalho VL,da Silva SP,Cardoso JF,de Oliveira RS,da Silva Lemos P,Wood TG,Widen SGdoi
10.1016/j.virol.2017.01.022subject
Has Abstractpub_date
2017-04-01 00:00:00pages
152-167eissn
0042-6822issn
1096-0341pii
S0042-6822(17)30031-4journal_volume
504pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Monoclonal antibodies to avian sarcoma/leukosis virus-coded transforming, structural, and polymerase proteins have been developed. In this first communication, a monoclonal antibody to pp60src will be characterized. The antibody can bind to pp60src in radioimmunoassays, precipitates this antigen in protein kinase test...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90440-9
更新日期:1984-02-01 00:00:00
abstract::Autoradiography of dodecyl sulfate-impregnated polyacrylamide gels after electrophoresis of the proteins from 32P-labeled cauliflower mosaic virus (CaMV) showed that two of the structural proteins were phosphorylated. These two proteins of 44 and 58 kilodaltons (kd) occur as minor components with some strains of the v...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90294-9
更新日期:1980-11-01 00:00:00
abstract::The nucleocapsid (N) protein genes of vesicular stomatitis virus, serotypes New Jersey and Indiana, were expressed in Escherichia coli BL21(DE3) transformed by the recombinant expression vector pET-3a containing N genes under the control of the T7 RNA polymerase promoter. Following induction with isopropylthiogalactop...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1130
更新日期:1993-03-01 00:00:00
abstract::The mouse mammary tumor virus (MMTV) is a type B retrovirus that is unique from other retroviruses in having multiple "tissue specific" and "hormone inducible" promoters. This unique feature has lead to the increasing interest in studying the biology of MMTV replication with the ultimate goal of developing MMTV based ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.12.027
更新日期:2009-03-15 00:00:00
abstract::The complete nucleotide sequence of the RSV cpts-248/404 live attenuated vaccine candidate was determined from cloned cDNA and was compared to that of the RSV A2/HEK7 wild-type, cold-passaged cp-RSV, and cpts-248 virus, which constitute the series of progenitor viruses. RSV cpts-248/404 is more attenuated and more tem...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0618
更新日期:1996-11-15 00:00:00
abstract::Monoclonal antibody 1B4, previously shown to be protective in vivo and to cross-react with both virally encoded and normal host cell proteins, was used to screen a lambda gt11 cDNA derived from mRNA harvested from mouse embryo fibroblasts 24 hr after infection with murine cytomegalovirus (MCMV). A 700-bp cDNA was iden...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1227
更新日期:1994-05-01 00:00:00
abstract::We have used a yeast two-hybrid system to identify a baculoviral gene encoding a protein kinase-interacting protein (PKIP). The pkip gene is located at 15.8-16.2 m.u (map units) and represents ORF 24 in the sequence of Ayres et al. (1994) of the Autographa californica nuclear polyhedrosis virus. PKIP is a 19.2-kDa pro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8944
更新日期:1998-01-20 00:00:00
abstract::Covalently closed circular dimers of phage lambda DNA have been found in Escherichia coli infected with lambda. These dimers can be formed by either the lambda Red or Int systems, by a nonrecombinational replicative mechanism requiring the activity of the lambda O and P genes or by joining of the cohesive ends. Dimers...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(77)90136-2
更新日期:1977-09-01 00:00:00
abstract::Influenza A virus, a respiratory pathogen manipulates various host cellular processes to establish a successful infection in a host. We had reported earlier the interaction of influenza A virus nucleoprotein with host glycolytic enzymes; alpha enolase and pyruvate kinase in A549 cells. Matrix protein (M1), another mul...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2020.07.019
更新日期:2020-10-01 00:00:00
abstract::Small non-coding microRNAs (miRNAs) can modulate the outcome of virus infection. Here we explore the role of miRNAs in insect-virus interactions, in vivo, using the natural Drosophila melanogaster-Drosophila C virus (DCV) model system. Comparison of the miRNA expression profiles in DCV-infected and uninfected flies sh...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.12.009
更新日期:2017-02-01 00:00:00
abstract::While the 2009 pandemic H1N1 virus has become established in the human population as a seasonal influenza virus, continued adaptation may alter viral virulence. Here, we passaged a 2009 pandemic H1N1 virus (A/Changchun/01/2009) in mice. Serial passage in mice generated viral variants with increased virulence. Adapted ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.09.014
更新日期:2015-12-01 00:00:00
abstract::By the use of reverse transcription followed by polymerase chain reaction (RT-PCR), we have identified one shorter than full-length, pregenomic viral RNA species in liver samples of woodchucks chronically infected with the woodchuck hepatitis virus (WHV). The spliced WHV RNA of about 2.4 kb in length was cloned and pa...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)91205-9
更新日期:1992-09-01 00:00:00
abstract::During replication of human parainfluenza virus type 3 (HPIV3), the 96-nucleotide antigenomic promoter (AGP) of HPIV3 directs the synthesis of genomic RNA. Previous work showed that nucleotides 1-12 were critical in promoting replication of an HPIV3 minireplicon, but nucleotides 13-96 were not investigated. In this st...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.08.003
更新日期:2011-10-25 00:00:00
abstract::Puumala (PUUV) and Hantaan (HTNV) viruses are hantaviruses within the family Bunyaviridae and associated with Hemorrhagic Fever with Renal Syndrome (HFRS) in humans. Little is known about how these viruses interact with host cells, though pathogenic hantaviruses interact with α(v)β(3) integrin. To study host cell inte...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.08.012
更新日期:2012-02-20 00:00:00
abstract::Turnip crinkle virus (TCV) (family Tombusviridae, genus Carmovirus) is a positive-sense RNA virus containing a 4054-base genome. Previous results indicated that insertion of Hairpin 4 (H4) into a TCV-associated satellite RNA enhanced replication 6-fold in vivo (Nagy, P., Pogany, J., Simon, A. E., 1999. EMBO J. 18:5653...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.03.051
更新日期:2006-08-15 00:00:00
abstract::In order to investigate the global and dynamic host microRNAs (miRNAs)/messenger RNAs (mRNAs) expression alteration during in vitro acute HCV infection, a comprehensive microarray analysis was performed using human hepatoma cells. Totally, 108 human miRNAs and 1247 mRNAs were identified whose expression levels changed...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.11.036
更新日期:2010-03-01 00:00:00
abstract::Red sea bream iridovirus (RSIV) of the Iridoviridae family is a causative agent of lethal infections in many cultured marine fish species in southwestern Japan. RSIV-induced apoptosis was divided as follows: (1). cell shrinkage and rounding at the early apoptotic stage, (2). cell enlargement at the middle apoptotic st...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.02.006
更新日期:2004-05-01 00:00:00
abstract::Characterization of the regions of human papillomaviruses (HPVs) that elicit neutralizing immune responses supports studies on viral infectivity and provides insight for the development and evaluation of prophylactic vaccines. HPV11 is a major etiologic agent of genital warts and a likely vaccine candidate. A conforma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0083
更新日期:2000-01-20 00:00:00
abstract::The rep gene of the defective human parvovirus, adeno-associated virus, (AAV) mediates several trans-acting functions important to virus replication, transcription, and gene expression. At least four overlapping polypeptides are expressed from the rep gene. We have constructed a prokaryotic vector which expressed in E...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90166-8
更新日期:1987-11-01 00:00:00
abstract::Guinea pig cytomegalovirus (GPCMV) immediate early proteins, IE1 and IE2, demonstrated structural and functional homologies with human cytomegalovirus (HCMV). GPCMV IE1 and IE2 co-localized in the nucleus with each other, the viral polymerase and guinea pig ND10 components (gpPML, gpDaxx, gpSp100, gpATRX). IE1 showed ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.01.023
更新日期:2017-04-01 00:00:00
abstract::The neuraminidase (NA) genes of influenza B viruses B/Maryland/59, B/Hong Kong/8/73, B/Singapore/222/79, B/Oregon/5/80, B/USSR/100/83, B/Victoria/3/85, B/Leningrad/179/86, B/Memphis/6/86, and B/Memphis/3/89 have been sequenced. The deduced amino acid sequences show high variability in the stalk domain of the NA, but a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90523-t
更新日期:1990-08-01 00:00:00
abstract::Magnetic birefringence measurements are reported on solutions of two double-stranded DNA containing viruses: icosahedral cauliflower mosaic virus (CaMV) and SD, an isometric bacteriophage with a short tail. Assuming the signal comes predominantly from the DNA, constraints can be put on its packing geometry. The anisot...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90232-1
更新日期:1986-12-01 00:00:00
abstract::The HPIV2 V protein inhibits type I interferon (IFN) induction and signaling. To manipulate the V protein, whose coding sequence overlaps that of the polymerase-associated phosphoprotein (P), without altering the P protein, we generated an HPIV2 virus in which P and V are expressed from separate genes (rHPIV2-P+V). rH...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.07.011
更新日期:2010-10-10 00:00:00
abstract::This study was initiated to evaluate the in vivo infectivity and pathogenicity of a group of recombinant feline leukemia viruses (rFeLVs) previously generated by in vitro forced recombination between a FeLV subgroup A virus (FeLV-A) and an endogenous FeLV (enFeLV) envelope (env) element (Sheets et al., 1992, Virology ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.0069
更新日期:1995-12-20 00:00:00
abstract::Epstein-Barr virus nuclear antigen 1 (EBNA 1) has been shown to be a sequence-specific DNA binding protein that is required for the replication of episomal elements carrying the viral origin of DNA replication, oriP, as well as for the activation of a specific transcriptional enhancer. We have constructed and analyzed...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90461-w
更新日期:1992-04-01 00:00:00
abstract::Putative replication complexes of cauliflower mosaic virus (CaMV) which resemble hepatitis B virus replication complexes in several respects have been identified. The CaMV replication complexes contain a heterogeneous population of minus-strand DNA ranging in size from less than 600 nucleotides to full genome length o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90109-6
更新日期:1985-05-01 00:00:00
abstract::We describe studies of woodchuck hepatitis virus nucleic acids in liver and other tissues of chronically infected woodchucks, using Southern and Northern blot hybridization techniques. Single-stranded and covalently closed circular replicative DNA molecules were distinguished from partly double-stranded virus genomes....
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90035-4
更新日期:1989-03-01 00:00:00
abstract::Although lentiviruses like HIV-1 are able to infect non-dividing cells, particular resting cells such as non-stimulated primary peripheral blood mononuclear cells (PBMC) are resistant to infection. In contrast to other lentiviruses, SIVsmmPBj can replicate in non-stimulated PBMC. Moreover, SIVsmmPBj-derived, but not H...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.03.008
更新日期:2007-08-01 00:00:00
abstract::Viral loads were investigated in SIVmnd-1 chronically infected mandrills and the results were compared with those previously observed in other nonpathogenic natural SIV infections. Four naturally and 11 experimentally SIVmnd-1-infected mandrills from a semi-free-ranging colony were studied during the chronic phase of ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.08.015
更新日期:2003-12-05 00:00:00
abstract::Adenovirus early region 1A (E1A) products are phosphorylated nuclear oncoproteins which appear to derive transforming activity largely through interactions with cellular proteins including the tumor suppressor p105/Rb-1 and cyclin A (p60cycA), a regulatory subunit associated with p34cdc2 and the related protein kinase...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90686-j
更新日期:1992-07-01 00:00:00