Abstract:
:The nucleocapsid (N) protein genes of vesicular stomatitis virus, serotypes New Jersey and Indiana, were expressed in Escherichia coli BL21(DE3) transformed by the recombinant expression vector pET-3a containing N genes under the control of the T7 RNA polymerase promoter. Following induction with isopropylthiogalactopyranoside, the N protein was synthesized, representing 10 to 20% of total cellular protein. The N protein remained in a nonaggregated form in 1 M NaCl solution. The purified N protein was found to be biologically active as demonstrated by its encapsidation of preformed leader RNA as well as its synthesis of genome length RNA in an in vitro transcription-replication reaction. The availability of recombinant N protein in a sufficient amount would facilitate structure and function studies of this important viral protein.
journal_name
Virologyjournal_title
Virologyauthors
Das T,Banerjee AKdoi
10.1006/viro.1993.1130subject
Has Abstractpub_date
1993-03-01 00:00:00pages
340-7issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(83)71130-Xjournal_volume
193pub_type
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