Abstract:
:We have previously cloned and analyzed the structure of a type 16 human papillomavirus (HPV16) integration in a primary cervical carcinoma tissue, M50 (Choo et al., J. Virol. 62, 1659-1666, 1988). We found that specific nucleotide sequences within the HPV16 genome influenced the genomic organization of the integrated viral genome. Using the viral-cellular junctions of the M50 DNA as probes, we have now cloned the unoccupied site from a human genomic library. Mapping analysis showed that a deletion of about 1.1 kilobase pairs (kb) had occurred at the integration site of M50. Sequencing of the integration junctions of the unoccupied site and comparison with the viral sequence has revealed short regions of sequence homology between the viral and the cellular genomes at both junctions. Our results are consistent with a mechanism of integration of the HPV16 sequences in the M50 carcinoma involving illegitimate recombination events using short patches of homologous sequences between the two heterologous genomes for anchorage and as guides for crossover. Preferred topoisomerase I cleavage sites and alternating purine and pyrimidine bases, which favor the formation of Z-DNA, could also be identified at the integration regions, supporting a proposed role for the topoisomerase I enzyme in the illegitimate recombination in the viral integration process.
journal_name
Virologyjournal_title
Virologyauthors
Choo KB,Lee HH,Liew LN,Chong KY,Chou HFdoi
10.1016/0042-6822(90)90366-ysubject
Has Abstractpub_date
1990-10-01 00:00:00pages
621-5issue
2eissn
0042-6822issn
1096-0341journal_volume
178pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Next generation sequence analyses were used to assess virus-derived small RNA (vsRNA) profiles for Homalodisca coagulata virus-1 (HoCV-1), family Dicistroviridae, and Homalodisca vitripennis reovirus (HoVRV), family Reoviridae, from virus-infected H. vitripennis, the glassy-winged sharpshooter. The vsRNA reads were ma...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.04.005
更新日期:2013-07-20 00:00:00
abstract::An effective type I interferon (IFN-alpha/beta) response is critical for the control of many viral infections. Using an oncolytic strain of vesicular stomatitis virus, we have examined the cross-talk between virus-induced apoptosis and initiation of innate immune response. The intrinsic apoptotic cascade, specifically...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.03.011
更新日期:2007-08-15 00:00:00
abstract::We studied experimental canine oral papillomavirus (COPV) infection by in situ hybridization and immunohistochemistry of weekly biopsies. After 4 weeks, viral DNA in rete ridges suggested a keratinocyte stem cell target. Abundant viral DNA was seen in E4-positive cells only. E4 was predominantly cytoplasmic but also n...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0868
更新日期:2001-05-25 00:00:00
abstract::The RTVL-H family of human endogenous retrovirus-like sequences consists of approximately 1000 "full-length" elements and at least as many solitary RTVL-H related long terminal repeats (LTRs). We have characterized cDNA clones from two human cell libraries (Hep-2 and normal peripheral blood) and have found three clone...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90570-9
更新日期:1989-12-01 00:00:00
abstract::Hepatitis C virus (HCV) NS5A is a phosphoprotein that possesses a cryptic trans-activation activity. To investigate its potential role in viral replication, we searched for the cellular proteins interacting with NS5A protein by yeast two-hybrid screening of a human hepatocyte cDNA library. We identified a newly discov...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9893
更新日期:1999-10-10 00:00:00
abstract::Pre-exposure prophylaxis (PrEP) strategies utilizing anti-retroviral drugs show considerable promise for HIV prevention. However there is insufficient pharmacokinetic (PK) data on drug concentrations required for protection at the relevant mucosal tissues where the infection is initiated. Here we evaluated the utility...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.07.008
更新日期:2014-09-01 00:00:00
abstract::A mouse model system for a lethal encephalitis due to influenza has been established by stereotaxic microinjection with the recombinant R404BP strain of influenza A virus into the olfactory bulb of C57BL/6 mice. The virus infection spread selectively to neurons in nuclei of the broad areas of the brain parenchyma that...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1601
更新日期:2002-11-25 00:00:00
abstract::Monoclonal antibodies specific for eight antigenic sites on the G1 glycoprotein of La Crosse virus were studied in a kinetics of neutralization assay. When examined at the same protein concentration, there were differences in the rates and degree of neutralization induced by these antibodies. The maximum amount of neu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90163-6
更新日期:1984-07-30 00:00:00
abstract::Adenovirus 12 (Ad12), but not adenovirus 2 or 5, induces metaphase chromosome fragility at four specific loci in humans: RNU1, RNU2, PSU1, and RN5S. As each of these sites corresponds to a tandemly repeated multigene family encoding a small, abundant structural RNA, we proposed that Ad12 hinders metaphase chromatin co...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9512
更新日期:1999-02-01 00:00:00
abstract::The effects of P2 prophage on lambda-mediated depression of host metabolism, and on lambda macromolecular synthesis have been studied. Experiments employing certain lambdabio deletion mutants and lambda-related phages suggest that the same gene products are involved in inhibition of host metabolism (Hin+ function) and...
journal_title:Virology
pub_type: 杂志文章
doi:
更新日期:1971-11-01 00:00:00
abstract::Adenovirus vectors are known to induce certain genes and impact innate response networks, but a broad understanding of this process and its mechanisms is currently lacking. For this reason, we chose to investigate and characterize Ad innate immunity using homogeneous, primary MEF cells replete with all the elements of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.08.041
更新日期:2007-02-20 00:00:00
abstract::HK97 is a temperate dsDNA bacteriophage of Escherichia coli that is structurally similar to phage lambda, with an icosahedral head of triangulation (7) number 7. Although the capsids of several large dsDNA phages have been studied extensively using a variety of biophysical approaches, no high-resolution structure is a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9040
更新日期:1998-03-30 00:00:00
abstract::We used Cre/loxP recombination to swap targeting ligands present on the adenoviral capsid protein IX (pIX). A loxP-flanked sequence encoding poly-lysine (pK-binds heparan sulfate proteoglycans) was engineered onto the 3'-terminus of pIX, and the resulting fusion protein allowed for routine virus propagation. Growth of...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.09.004
更新日期:2011-11-25 00:00:00
abstract::The eukaryotic translation initiation factor eIF4E, the cap-binding protein, seems to play an essential role in the establishment of the host shut-off after viral infection. Infection with adenovirus and influenza virus caused dephosphorylation of eIF4E and an involvement of a viral protein was suggested. In this repo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0143
更新日期:1996-03-15 00:00:00
abstract::Few human endogenous retroviruses (HERVs) have been extensively studied in non-human primates. Such investigations have demonstrated that several element classes are primate unique, contain members with important biological function, are conserved in specific primate lineages, and have in some cases expanded in copy n...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.01.045
更新日期:2005-04-10 00:00:00
abstract::We have carried out an exhaustive search for amino acid sequence similarities between vesicular stomatitis virus (VSV) proteins and database entries. Unexpectedly, we found that the L polymerase protein contains two blocks of sequence (residues 725-1102 and 1291-1671) with distant but statistically significant similar...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90148-7
更新日期:1989-09-01 00:00:00
abstract::HAV particles isolated from infected cells banded at buoyant densities of 1.42, 1.32, and 1.20 g/ml, and distinctive protein patterns were established by gel electrophoresis and reverse phase high performance liquid chromatography. The relatively higher amounts of p30 in particles with lower buoyant densities suggest ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90234-5
更新日期:1986-12-01 00:00:00
abstract::The mechanism by which lentivirus envelope (Env) glycoproteins are packaged into budding virions is poorly understood. Simian immunodeficiency virus (SIV) contains an Env protein with an unusually long cytoplasmic tail. To investigate the role of this domain in the incorporation of the SIV Env into virions, we generat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.0869
更新日期:2001-05-10 00:00:00
abstract::Ten human genital invasive squamous cell carcinomas and five human premalignant tissues were analyzed for the presence of selected sets of herpes simplex virus 2 (HSV-2) DNA sequences. Two vulvar tumors and one vulvar dysplastic tissue were found to contain DNA sequences homologous to the BglII O fragment (coordinates...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90179-0
更新日期:1986-11-01 00:00:00
abstract::Nuclear Matrix and MARs have been implicated in the transcriptional regulation of host as well as viral genes but their precise role in HIV-1 transcription remains unclear. Here, we show that >98% of HIV sequences contain consensus MAR element in their promoter. We show that SMAR1 binds to the LTR MAR and reinforces t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.01.017
更新日期:2010-04-25 00:00:00
abstract::The nucleotide sequence of the genome of the nucleopolyhedrovirus (NPV) from Mamestra configurata (MacoNPV, isolate 90/2), a group II NPV, was determined and analyzed. The MacoNPV DNA genome consists of 155,060 bp and has an overall G+C content of 41.7%. Computer-assisted analysis predicted 169 open reading frames (OR...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1313
更新日期:2002-03-01 00:00:00
abstract::The glycoprotein (GP) of lymphocytic choriomeningitis virus (LCMV) serves as virus attachment protein to its receptor on host cells and is a key determinant for cell tropism, pathogenesis, and epidemiology of the virus. The GP of LCMV is posttranslationally cleaved by the subtilase SKI-1/S1P into two subunits, the per...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00421-5
更新日期:2003-09-15 00:00:00
abstract::Rift Valley fever virus-induced protein synthesis was examined by polyacrylamide gel electrophoresis and fluorography. Five virus-induced polypeptides were detected, the nucleocapsid protein N, the nucleus-associated nonstructural protein NS1, the glycoproteins G1 and G2, and a protein of molecular weight 80K. The N, ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90277-0
更新日期:1984-04-15 00:00:00
abstract::Cells that express the human papillomavirus (HPV) type 16 E7 oncoprotein are predisposed to undergo apoptosis. Transgenic mice that have E7 expression targeted to either the retinal photoreceptor cells or the lens cells exhibit signs of apoptosis in cells attempting to undergo differentiation. We established a cell cu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8851
更新日期:1997-12-08 00:00:00
abstract::During capsid assembly of bacteriophage P2 and its satellite phage P4, gpN undergoes proteolytic cleavage with the removal of the first 31 amino acids. The truncated protein gpN* is unable to support formation of viable phages in complementation tests. A c-myc antigenic epitope (EQKLISEEDL) exchanged for eleven amino ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0566
更新日期:1996-10-15 00:00:00
abstract::A short upstream open reading frame (uORF2) in the human cytomegalovirus (CMV) gpUL4 (gp48) transcript leader is conserved among CMV strains and inhibits translation of a downstream cistron. Remarkably, this inhibitory effect depends on the amino acid coding information of uORF2, at least in transient transfection ass...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1630
更新日期:1994-11-15 00:00:00
abstract::The Pr76Gag and Pr180Gag-Pol polyprotein precursors of Rous sarcoma virus contain a 22-amino-acid spacer peptide, called p2, located between the amino acid sequences of the mature Gag proteins MA and p10. This spacer peptide is present in stoichiometric amounts in the virion, albeit cleaved into two parts, but its fun...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1450
更新日期:1994-08-15 00:00:00
abstract::In order to establish whether the poor growth of the two fastidious adenoviruses types 40 and 41 (Ad40 and Ad41) in HeLa cells is due to a reduced trans-activation by the early region 1A (E1A), we have determined the trans-activating effect of this region on the expression of the chloramphenicol acetyltransferase (CAT...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90080-8
更新日期:1987-09-01 00:00:00
abstract::Rice yellow mottle virus (RYMV) is icosahedral in morphology and known to swell in vitro, but the biological function of swollen particles remains unknown. Anion-exchange chromatography was used to identify three markedly stable forms of RYMV particles from infected plants: (1) an unstable swollen form lacking Ca2+ an...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1398
更新日期:2002-05-25 00:00:00
abstract::Two major infectious forms of vaccinia virus (VACV) have been described: the intracellular mature virion (IMV), and the extracellular enveloped virion (EEV). Due to their stability in the environment, IMVs play a predominant role in host-to-host transmission, whereas EEVs play an important role in dissemination within...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(02)00038-7
更新日期:2003-02-01 00:00:00