Abstract:
:Nuclear Matrix and MARs have been implicated in the transcriptional regulation of host as well as viral genes but their precise role in HIV-1 transcription remains unclear. Here, we show that >98% of HIV sequences contain consensus MAR element in their promoter. We show that SMAR1 binds to the LTR MAR and reinforces transcriptional silencing by tethering the LTR MAR to nuclear matrix. SMAR1 associated HDAC1-mSin3 corepressor complex is dislodged from the LTR upon cellular activation by PMA/TNFalpha leading to an increase in the acetylation and a reduction in the trimethylation of histones, associated with the recruitment of RNA Polymerase II on the LTR. Overexpression of SMAR1 lead to reduction in LTR mediated transcription, both in a Tat dependent and independent manner, resulting in a decreased virion production. These results demonstrate the role of SMAR1 in regulating viral transcription by alternative compartmentalization of LTR between the nuclear matrix and chromatin.
journal_name
Virologyjournal_title
Virologyauthors
Sreenath K,Pavithra L,Singh S,Sinha S,Dash PK,Siddappa NB,Ranga U,Mitra D,Chattopadhyay Sdoi
10.1016/j.virol.2010.01.017subject
Has Abstractpub_date
2010-04-25 00:00:00pages
76-85issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(10)00034-6journal_volume
400pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::To elucidate the mechanism of formation of cowpea mosaic virus (CPMV) particles, RNA-2-encoded precursor proteins were expressed in Spodoptera frugiperda cells. Processing of the 105K and 95K polyproteins in trans to give the mature Large (L) and Small (S) coat proteins required both the 32K proteinase cofactor and th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.08.023
更新日期:2009-10-25 00:00:00
abstract::In this article we report the initial biochemical, genetic, and electron microscopic analysis of a previously uncharacterized, 8.9-kDa, predicted thiol-redox protein. The name A2.5L was assigned to the corresponding vaccinia virus gene, which is conserved in all sequenced poxviruses. Multiple alignment analysis and se...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1608
更新日期:2002-09-01 00:00:00
abstract::B-HIV1, an oligoclone of immortalized cells derived from human peripheral B lymphocytes infected in vitro with the TIIIB isolate of HIV-1, produces low levels of replication-competent HIV when propagated in 1% serum, but increases production > or = 5-fold after phorbol myristate acetate (PMA) exposure. Electron micros...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1499
更新日期:1993-10-01 00:00:00
abstract::Endogenous retroviruses (ERVs) arise from the infection and integration of past retroviruses into animal hosts. We performed large-scale genomic mining of 101 avian genomes for discovery of ERVs having none-avian origin and investigated the cross-species transmission events. Phylogenetic analysis of the reverse transc...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2019.02.005
更新日期:2019-04-01 00:00:00
abstract::Nanogram amounts of the proinflammatory cytokine interleukin-1alpha (IL-1alpha) were detected in uninfected cultures of human corneal epithelial cells (HCEC). Although HSV-1 replicated >10(4)-fold in these cells and caused extensive cytopathic effects, virus infection was not accompanied by significant extracellular r...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9084
更新日期:1998-04-25 00:00:00
abstract::Geminiviruses are small, ssDNA-containing plant viruses. Bean golden mosaic virus (BGMV) and tomato golden mosaic virus (TGMV) have bipartite genomes, the components of which are designated A and B. Although they are closely related, BGMV and TGMV nevertheless exhibit distinct host-specific phenotypes, with BGMV being...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9424
更新日期:1998-11-25 00:00:00
abstract::High-risk human papillomaviruses (HPVs) are etiologically implicated in human cervical cancer. Two viral genes, E6 and E7, are commonly found expressed in these cancer cells. We have previously shown that mice transgenic for the HPV-16 E6 gene or E7 gene, in which the E6 or E7 was expressed in the basal layer of epith...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0106
更新日期:2000-02-15 00:00:00
abstract::Virus infection may contribute to asthma pathogenesis. In turn, a Th2-polarized pulmonary environment may increase host susceptibility to infection. We used a cockroach antigen (CRA) model of allergic airway disease to test the hypothesis that Th2 cytokine overproduction increases susceptibility to mouse adenovirus ty...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.06.009
更新日期:2009-08-15 00:00:00
abstract::The amino-terminal domain of influenza A virus matrix protein (residues 1-164) was crystallized at pH 7 into a new crystal form in space group P1. This packing of the protein implies that M1(1-164) was monomeric in solution when it crystallized. Otherwise, the structure of the M1 fragment in the pH 7 crystals was the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0727
更新日期:2001-01-20 00:00:00
abstract::Each copy of the inverted terminal repeat of vaccinia virus consists of 8 kb of DNA containing 9 ORFS flanked near the terminus of the genome by 4 kb of repetitive DNA which in turn contains blocks of tandem repeats. Using plasmids containing repetitive DNA as the external arm, we have generated deletions at both the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90047-f
更新日期:1991-01-01 00:00:00
abstract::Spherical virus particles were purified from larval striped jack (Pseudocaranx dentex) with nervous necrosis. The virus consists of nonenveloped particles, about 25 nm in diameter, and contains two single-stranded, positive-sense RNA molecules with molecular weights of 1.01 x 10(6)Da (RNA 1) and 0.49 x 10(6)Da (RNA 2)...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90329-n
更新日期:1992-03-01 00:00:00
abstract::Ovine pulmonary carcinoma is a contagious neoplastic disease of sheep which can be transmitted experimentally with cell-free filtrate. Products homologous to structural proteins of type D and type B retroviruses are expressed in the tumors, but an infectious retrovirus has yet to be isolated. Retroviral RNA was isolat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1366
更新日期:1994-07-01 00:00:00
abstract::Replication of the satellite RNA of tobacco ringspot virus (sTobRV RNA) has been postulated to require rolling circle transcription. The expected product of rolling circle transcription, multimeric sTobRV RNA, is known to undergo self-cleavage in vitro to release unit-length sTobRV RNA. A spontaneous, efficient, not e...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90005-v
更新日期:1991-01-01 00:00:00
abstract::Attachment of human adenoviruses (Ads) to host cells is mediated by the interaction of the fiber protein of the capsid with specific cell-surface molecules. For one of the species B adenoviruses, Ad3, the mechanism of binding to cells remains to be defined. Several previous reports have proposed CD46, CD80 or CD86 as ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.07.010
更新日期:2009-09-30 00:00:00
abstract::In this study, we show that adenoviral infection induced accumulation of the sphingolipid ceramide in a dose- and time-dependent manner. This accumulation preceded cell lysis, occurred in the absence of biochemical evidence of apoptosis, and was derived from de novo synthesis of ceramide. An adenovirus mutant that lac...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.09.060
更新日期:2006-02-05 00:00:00
abstract::Human papillomaviruses (HPVs) infect epithelial cells and are associated with genital carcinoma. Most epithelial cell lines express cell-surface glycosaminoglycans (GAGs) usually found attached to the protein core of proteoglycans. Our aim was to study how GAGs influenced HPV entry. Using a human keratinocyte cell lin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00114-4
更新日期:2003-05-25 00:00:00
abstract::The mutations in two DNA-negative ts mutants of herpes simplex virus type 1 (HSV-1), tsK13 and tsM19, have been previously mapped to a 2.0-kb fragment (coordinates 0.095-0.108) at the left end of the genome (S. Weller, D. Aschman, W. Sacks, D. Coen, and P. Schaffer, 1983, Virology 130, 290-305). Sequence analysis of t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90507-7
更新日期:1988-10-01 00:00:00
abstract::Persistent infection with the Edmonston strain of measles virus (MV) has been established in IC-21 cells, an immortalized murine macrophage cell line. Persistence was established immediately without syncytia formation or cytopathic effects. MV was expressed in the majority of the cells as evidenced by immunofluorescen...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1047
更新日期:1995-02-20 00:00:00
abstract::A subtype of human papillomavirus 5 (HPV-5b) is closely associated with carcinomas in the disease epidermodysplasia verruciformis (EV). The complete genome was cloned from virus particles in benign lesions of a patient with EV and sequenced: it was 7779 nucleotides long and consisted of six open reading frames (ORFs) ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)91013-7
更新日期:1991-08-01 00:00:00
abstract::Genotype VI-paramyxovirus (GVI-PMV1) is a major cause of epidemic Newcastle-like disease in Columbiformes. This genotype of avian paramyxovirus type 1 has diversified rapidly since its introduction into the US in 1982 resulting in two extant lineages, which have different population growth properties. Although some GV...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.06.007
更新日期:2014-08-01 00:00:00
abstract::Simian virus (SV40) T antigen shares many characteristics with adenovirus E1A which is known to induce apoptosis. To verify the potential of SV40 T antigen-mediated apoptosis, we stably expressed T antigen in immortalized human epithelial cells (Z172 and HaCaT). We found that SV40 T antigen could directly cause apopto...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9109
更新日期:1998-05-10 00:00:00
abstract::In vitro translation of pH 8-washed particles of tobacco mosaic virus (TMV; vulgare, common, or U1 strain) in a cell-free system derived from Escherichia coli (MRE 600) results in a broad size range of TMV RNA-encoded products, including significant amounts of the distinctive 126-kDa polypeptide which is encoded by th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90394-6
更新日期:1986-07-15 00:00:00
abstract::The interaction of reovirus with the cytoskeleton was investigated. The soluble components of infected cells were extracted with the nonionic detergent NP-40 in a physiological buffer, and a cytoskeletal extract was prepared from the detergent-insoluble fraction. We observed a selective association of viral-specified ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90464-8
更新日期:1987-08-01 00:00:00
abstract::The natural host of SIVrcm is the red-capped mangabey (Cercocebus torquatus torquatus). Although this virus infects macaques and human PBMCs, its pathogenic potential is unknown. We serially passaged SIVrcm through 9 rhesus macaques to assess its potential for virulence. SIVrcm infected all macaques with peak viremia ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.012
更新日期:2008-09-15 00:00:00
abstract::The V3 region of the HIV-1 envelope (Env) glycoprotein gp120 is a key functional domain yet it exhibits distinct mutational patterns across subtypes. Here an invariant residue (Ile 309) was replaced with Leu in 7 subtype C patient-derived Envs from recent infection and 4 related neutralizing antibody escape variants t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.04.010
更新日期:2010-08-15 00:00:00
abstract::Flock house virus (FHV) is the best studied member of the Nodaviridae, a family of small, nonenveloped, isometric RNA viruses of insects and fish. Nodavirus genomes comprise two single-stranded positive-sense RNA segments (RNAs 1 and 2) that encode the viral RNA-dependent RNA polymerase (RdRp) and capsid protein precu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1125
更新日期:2001-10-25 00:00:00
abstract::Recently, cases of severe respiratory illness in military and civilian populations have been associated with a new genomic variant of adenovirus (Ad) serotype 14, designated Ad14a. Compared to the Ad14 reference strain (de Wit), this new virus had a deletion of two amino acid residues in the fiber protein knob. Here w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.02.034
更新日期:2009-05-10 00:00:00
abstract::The Rta (R transactivator) protein plays an essential role in the Epstein-Barr viral (EBV) lytic cascade. Rta activates viral gene expression by several mechanisms including direct and indirect binding to target viral promoters, synergy with EBV ZEBRA protein, and stimulation of cellular signaling pathways. We previou...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.01.022
更新日期:2009-04-10 00:00:00
abstract::In this report, we present the DNA sequence and transcriptional characterization of a gene (IR5) that maps within each of the inverted repeat (IR) segments of the equine herpesvirus type 1 (EHV-1) genome. The IR5 open reading frame (ORF) is located within both IR sequences (nucleotides 9932-10,642 of the IR). DNA sequ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90525-t
更新日期:1992-06-01 00:00:00
abstract::The E7 oncoprotein of high risk human papillomavirus type 16 (HPV16) binds and inactivates the retinoblastoma (RB) family of proteins. Our previous studies suggested that HPV16 E7 enters the nucleus via a novel Ran-dependent pathway independent of the nuclear import receptors (Angeline, M., Merle, E., and Moroianu, J....
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.09.037
更新日期:2009-01-05 00:00:00