Abstract:
:HAV particles isolated from infected cells banded at buoyant densities of 1.42, 1.32, and 1.20 g/ml, and distinctive protein patterns were established by gel electrophoresis and reverse phase high performance liquid chromatography. The relatively higher amounts of p30 in particles with lower buoyant densities suggest that this protein is VP0 and is part of the immature picornavirion. The protein elution profiles obtained by HPLC were virtually identical for all the HAV strains examined but differed from those of other picornaviruses. The N-terminal amino acid sequence of VP1 and VP2 was determined and aligned to the nucleotide sequence. Sequencing VP0 and VP3 was not possible, probably because the amino termini are blocked. VP1, VP3, and VP0 induced specific antibodies in rabbits.
journal_name
Virologyjournal_title
Virologyauthors
Gauss-Müller V,Lottspeich F,Deinhardt Fdoi
10.1016/0042-6822(86)90234-5subject
Has Abstractpub_date
1986-12-01 00:00:00pages
732-6issue
2eissn
0042-6822issn
1096-0341journal_volume
155pub_type
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