Abstract:
:In order to establish whether the poor growth of the two fastidious adenoviruses types 40 and 41 (Ad40 and Ad41) in HeLa cells is due to a reduced trans-activation by the early region 1A (E1A), we have determined the trans-activating effect of this region on the expression of the chloramphenicol acetyltransferase (CAT) gene controlled by the Ad2 E4 promoter. Cotransfection of HeLa cells with plasmids containing the E1A regions of Ad5, Ad40, and Ad41, respectively, and the CAT gene controlled by the Ad2 E4 promoter showed that activation of the E4 promoter by the E1A regions of Ad40 and Ad41 depends on the same sequence elements of the E4 promoter as activation by the Ad5 E1A gene products. The level of activation, however, is significantly lower. This might partly explain the reduced growth in HeLa cells of the two viruses.
journal_name
Virologyjournal_title
Virologyauthors
van Loon AE,Gilardi P,Perricaudet M,Rozijn TH,Sussenbach JSdoi
10.1016/0042-6822(87)90080-8subject
Has Abstractpub_date
1987-09-01 00:00:00pages
305-7issue
1eissn
0042-6822issn
1096-0341journal_volume
160pub_type
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