HIV-specific humoral and cellular immunity in rabbits vaccinated with recombinant human immunodeficiency virus-like gag-env particles.

abstract：:Human embryo lung cells pretreated with a combination of human leukocyte interferon and acyclovir were infected with human cytomegalovirus (HCMV) and treated daily for 14 days with the same inhibitor combination. After removal of the inhibitors, the incubation temperature was maintained at either 37 or 40.5 degrees. I...

journal_title：Virology

authors： Bucher LJ,Wigdahl B,Rapp F

更新日期：1983-10-15 00:00:00

abstract：:Long-term non-progressive HIV infection, characterized by low but detectable viral load and stable CD4 counts in the absence of antiviral therapy, is observed in about 5% of HIV-infected patients. Here we identified four therapy naïve individuals who are strongly seropositive for HIV-1 but who lack evidence of detecta...

journal_title：Virology

authors： Kloosterboer N,Groeneveld PH,Jansen CA,van der Vorst TJ,Koning F,Winkel CN,Duits AJ,Miedema F,van Baarle D,van Rij RP,Brinkman K,Schuitemaker H

更新日期：2005-08-15 00:00:00

abstract：:The effect of adenovirus infection or transformation on the DNA replication of Minute Virus of Mice (MVM) was studied in human fibroblast cell lines. In WI38, HeLa, and 293 cells MVM infection allowed production of viral NS-1 and capsid proteins with or without adenovirus 2 (Ad2) co-infection. However, MVM DNA replica...

journal_title：Virology

authors： Fox E,Moen PT Jr,Bodnar JW

更新日期：1990-06-01 00:00:00

abstract：:We investigated the effect of selection pressures on evolution of HIV-1 pol in 51 patients after switching to a new antiretroviral combination reverse transcriptase (RT) inhibitor therapy. Evolution of the protease (PR) and RT reading frames were analysed separately. Pairwise evolutionary distances (ED) were calculate...

journal_title：Virology

authors： Günthard HF,Leigh-Brown AJ,D'Aquila RT,Johnson VA,Kuritzkes DR,Richman DD,Wong JK

更新日期：1999-06-20 00:00:00

abstract：:Despite recent progress in the development of direct-acting antivirals against hepatitis C virus (HCV), chronic HCV infection remains an important health burden worldwide. MicroRNA122 (miR122), a liver-specific microRNA (miRNA), positively regulates HCV replication, and systemic application of antisense oligonucleotid...

journal_title：Virology

authors： Shibata C,Ohno M,Otsuka M,Kishikawa T,Goto K,Muroyama R,Kato N,Yoshikawa T,Takata A,Koike K

更新日期：2014-08-01 00:00:00

abstract：:Poliovirus was first isolated nearly 100 years ago in a landmark experiment that established the viral etiology of poliomyelitis. This discovery stimulated investigation of the pathogenesis of poliomyelitis in many laboratories. Nearly 50 years later, when two effective poliovirus vaccines were developed, the impetus ...

journal_title：Virology

authors： Racaniello VR

更新日期：2006-01-05 00:00:00

abstract：:Economic loss due to viral endothelial cell necrosis of eel (VECNE) of Anguilla japonica is a serious problem for the cultured Japanese eel market. However, the viral genome responsible for VECNE is unknown. We recently developed a rapid determination system for viral nucleic acid sequences (RDV) to determine viral ge...

journal_title：Virology

authors： Mizutani T,Sayama Y,Nakanishi A,Ochiai H,Sakai K,Wakabayashi K,Tanaka N,Miura E,Oba M,Kurane I,Saijo M,Morikawa S,Ono S

更新日期：2011-03-30 00:00:00

abstract：:Herpes simplex virus 1 (HSV-1) is a widespread pathogen that persists for life, replicating in surface tissues and establishing latency in peripheral ganglia. Increasingly, molecular studies of latency use cultured neuron models developed using recombinant viruses such as HSV-1 GFP-US11, a derivative of strain Patton ...

journal_title：Virology

authors： Pourchet A,Copin R,Mulvey MC,Shopsin B,Mohr I,Wilson AC

更新日期：2017-12-01 00:00:00

abstract：:Highly pathogenic alphaviruses display complex glycans on their surface. These glycans play a crucial role in viral pathogenesis by facilitating glycan-host interaction during viral entry which can be targeted. Various studies have reported antiviral activity of lectins that bind to the glycans present on the surface ...

journal_title：Virology

authors： Kaur R,Neetu,Mudgal R,Jose J,Kumar P,Tomar S

更新日期：2019-01-02 00:00:00

abstract：:The efficacy of combining immunization with human immunodeficiency vitus type 1 (HIV-1) DNA and HIV-1 recombinant proteins to obtain protection from chimeric simian/human immunodeficiency virus (SHIV) was determined. Four cynomolgus monkeys received four gene-gun immunizations intraepidermally of plasmid DNA encoding ...

journal_title：Virology

authors： Putkonen P,Quesada-Rolander M,Leandersson AC,Schwartz S,Thorstensson R,Okuda K,Wahren B,Hinkula J

更新日期：1998-10-25 00:00:00

abstract：:The paramyxovirus fusion protein (F) promotes fusion of the viral envelope with the plasma membrane of target cells as well as cell-cell fusion. The plasma membrane is closely associated with the actin cytoskeleton, but the role of actin dynamics in paramyxovirus F-mediated membrane fusion is unclear. We examined cell...

journal_title：Virology

authors： Schowalter RM,Wurth MA,Aguilar HC,Lee B,Moncman CL,McCann RO,Dutch RE

更新日期：2006-07-05 00:00:00

abstract：:Human papillomaviruses (HPVs) infect epithelial cells and are associated with genital carcinoma. Most epithelial cell lines express cell-surface glycosaminoglycans (GAGs) usually found attached to the protein core of proteoglycans. Our aim was to study how GAGs influenced HPV entry. Using a human keratinocyte cell lin...

journal_title：Virology

authors： Drobni P,Mistry N,McMillan N,Evander M

更新日期：2003-05-25 00:00:00

abstract：:Chlorella virus PBCV-1 encodes two putative chitinase genes, a181/182r and a260r, and one chitosanase gene, a292l. The three genes were cloned and expressed in Escherichia coli. The recombinant A181/182R protein has endochitinase activity, recombinant A260R has both endochitinase and exochitinase activities, and recom...

journal_title：Virology

authors： Sun L,Adams B,Gurnon JR,Ye Y,Van Etten JL

更新日期：1999-10-25 00:00:00

abstract：:The human parvovirus B19 causes aplastic crises in sickle cell anemia patients and the disease erythema infectiosum. So far, it has not been possible to grow B19 virus in cultured cells. Here we report the use of in vitro transcription in HeLa cell extracts and transient expression of cloned DNA transfected into HeLa ...

journal_title：Virology

authors： Doerig C,Beard P,Hirt B

更新日期：1987-04-01 00:00:00

abstract：:Replication of the satellite RNA of tobacco ringspot virus (sTobRV RNA) has been postulated to require rolling circle transcription. The expected product of rolling circle transcription, multimeric sTobRV RNA, is known to undergo self-cleavage in vitro to release unit-length sTobRV RNA. A spontaneous, efficient, not e...

journal_title：Virology

authors： Van Tol H,Buzayan JM,Bruening G

更新日期：1991-01-01 00:00:00

abstract：:Incubation of membrane vesicles from normal and Rous sarcoma virus-transformed chick embryo fibroblasts (CEF) with [gamma-32P]ATP resulted in the phosphorylation of a large number of proteins. The major differences observed between the membrane vesicles of untransformed and transformed cells were: (1) a 5- to 10-fold ...

journal_title：Virology

authors： Garber EA,Krueger JG,Hanafusa H,Goldberg AR

更新日期：1983-04-15 00:00:00

abstract：:Old world hantaviruses, causing hemorrhagic fever with renal syndrome (HFRS), still present a public health problem in Asia and Eastern Europe. The majority of cases has been recorded in China. The aim of our study was to generate human recombinant neutralizing antibodies to a hantavirus by phage display technology. T...

journal_title：Virology

authors： Koch J,Liang M,Queitsch I,Kraus AA,Bautz EK

更新日期：2003-03-30 00:00:00

abstract：:Hepatitis B viruses replicate via reverse transcription of an RNA intermediate. This RNA pregenome serves as mRNA and is packaged into capsids and reverse transcribed. Both processes require the interaction of the viral reverse transcriptase, P protein, with the 5'-proximal epsilon-signals on the pregenome. For epsilo...

journal_title：Virology

authors： Beck J,Bartos H,Nassal M

更新日期：1997-01-20 00:00:00

abstract：:We examined the molecular epidemiology and evolution of Ross River (RR) virus in Australia and the Pacific Islands. Nucleotide sequences of the E2 and E3 genes of five RR virus strains revealed remarkable conservation between 1959 and 1989 with a maximum divergence of only 3.3%. Sequence data from a 505-base pair frag...

journal_title：Virology

authors： Sammels LM,Coelen RJ,Lindsay MD,Mackenzie JS

更新日期：1995-09-10 00:00:00

abstract：:We constructed a sensitive and quantitative assay system to examine human T-cell leukemia virus type I (HTLV-I) envelope (env) glycoprotein-mediated cell fusion in which T7 RNA polymerase in donor cells coexpressing env glycoproteins activates a reporter gene in recipient cells upon cell fusion. An efficient expressio...

journal_title：Virology

authors： Okuma K,Nakamura M,Nakano S,Niho Y,Matsuura Y

更新日期：1999-02-15 00:00:00

abstract：:Varicella-zoster virus (VZV) open reading frame 61 (ORF61) protein transactivates expression of VZV promoters. VZV ORF61 is functionally homologous to herpes simplex virus type 1 (HSV-1)-infected cell protein 0 (ICP0); however, amino acid sequence homology of these two proteins is limited to the RING finger domains, a...

journal_title：Virology

authors： Moriuchi H,Moriuchi M,Cohen JI

更新日期：1994-11-15 00:00:00

abstract：:Two genetically distinct infectious recombinant virus clones (pMLV, constructed from Ingelvac PRRS MLV and pMN184, constructed from virulent strain MN184) were developed to study attenuation of contemporary porcine reproductive and respiratory syndrome virus (PRRSV) strain MN184. Two reciprocal chimeric clones (pMLVOR...

journal_title：Virology

authors： Wang Y,Liang Y,Han J,Burkhart KM,Vaughn EM,Roof MB,Faaberg KS

更新日期：2008-02-20 00:00:00

abstract：:Red clover necrotic mosaic virus (RCNMV) encodes N-terminally overlapping proteins of 27 and 88 kDa (p27 and p88) known to be required for replication. Green fluorescent protein (GFP) fusions were used to visualize the location of p27 and p88 within Nicotiana benthamiana cells. GFP:p27 fusions localized to the endopla...

journal_title：Virology

authors： Turner KA,Sit TL,Callaway AS,Allen NS,Lommel SA

更新日期：2004-03-15 00:00:00

abstract：:Cytoplasmic tails of envelope (Env) glycoproteins of many retroviruses inhibit their membrane fusion activity. The cytoplasmic 16-amino acid peptide of ecotropic murine leukemia virus (E-MLV) Env protein, called the R-peptide, also inhibits the membrane fusion activity of the Env protein. However, the molecular mechan...

journal_title：Virology

authors： Kubo Y,Izumida M,Togawa K,Zhang F,Hayashi H

更新日期：2019-06-01 00:00:00

abstract：:To identify ranavirus virulence genes, we engineered Frog Virus 3 (FV3) knockout (KO) mutants defective for a putative viral caspase activation and recruitment domain-containing (CARD) protein (Δ64R-FV3) and a β-hydroxysteroid dehydrogenase homolog (Δ52L-FV3). Compared to wild type (WT) FV3, infection of Xenopus tadpo...

journal_title：Virology

authors： Andino Fde J,Grayfer L,Chen G,Chinchar VG,Edholm ES,Robert J

更新日期：2015-11-01 00:00:00

abstract：:Analyses of reassortant and parental strains of BTV serotypes 3 and 10, in serum neutralization tests, confirmed the major role of outer capsid protein VP2 in determination of virus serotype and its involvement in serum neutralization. However, a reassortant BTV strain (R70), containing protein VP5 derived from BTV 3 ...

journal_title：Virology

authors： Mertens PP,Pedley S,Cowley J,Burroughs JN,Corteyn AH,Jeggo MH,Jennings DM,Gorman BM

更新日期：1989-06-01 00:00:00

abstract：:Retroviral Gag proteins function during early and late stages of the viral life cycle. To gain additional insight into the cellular requirements for viral replication, a two-hybrid screen was used to identify cellular proteins that interact with the Mason-Pfizer monkey virus Gag protein. One of the cellular proteins f...

journal_title：Virology

authors： Weldon RA Jr,Sarkar P,Brown SM,Weldon SK

更新日期：2003-09-15 00:00:00

abstract：:Gene transfer approaches using viruses such human adenovirus (HAdV) may provide an alternative treatment for diseases involving hematopoietic cells. Better understanding of the cellular mechanisms by which the HAdV introduces DNA into these cells should help in vector design. We examined HAdV intracellular delivery in...

journal_title：Virology

authors： Colin M,Renaut L,Mailly L,D'Halluin JC

更新日期：2004-03-01 00:00:00

abstract：:The morbilliviruses are a closely related genus which are very similar in their sequences and share a common receptor, but nevertheless show significant restriction in the host species in which they cause disease. One contribution to this restriction might be the nature of the hosts' responses to infection. We have us...

journal_title：Virology

authors： Nanda SK,Baron J,Royall E,Robinson L,Falciani F,Baron MD

更新日期：2009-12-20 00:00:00

abstract：:Efferocytosis, the phagocytic clearance of apoptotic cells, can provide host protection against certain types of viruses by mediating phagocytic clearance of infected cells undergoing apoptosis. It is known that HIV-1 induces apoptosis and HIV-1-infected cells are efferocytosed by macrophages, although its molecular m...

journal_title：Virology

authors： Chua BA,Ngo JA,Situ K,Ramirez CM,Nakano H,Morizono K

更新日期：2018-02-01 00:00:00