Abstract:
:Herpes simplex virus 1 (HSV-1) is a widespread pathogen that persists for life, replicating in surface tissues and establishing latency in peripheral ganglia. Increasingly, molecular studies of latency use cultured neuron models developed using recombinant viruses such as HSV-1 GFP-US11, a derivative of strain Patton expressing green fluorescent protein (GFP) fused to the viral US11 protein. Visible fluorescence follows viral DNA replication, providing a real time indicator of productive infection and reactivation. Patton was isolated in Houston, Texas, prior to 1973, and distributed to many laboratories. Although used extensively, the genomic structure and phylogenetic relationship to other strains is poorly known. We report that wild type Patton and the GFP-US11 recombinant contain the full complement of HSV-1 genes and differ within the unique regions at only eight nucleotides, changing only two amino acids. Although isolated in North America, Patton is most closely related to Asian viruses, including KOS63.
journal_name
Virologyjournal_title
Virologyauthors
Pourchet A,Copin R,Mulvey MC,Shopsin B,Mohr I,Wilson ACdoi
10.1016/j.virol.2017.09.016subject
Has Abstractpub_date
2017-12-01 00:00:00pages
124-131eissn
0042-6822issn
1096-0341pii
S0042-6822(17)30325-2journal_volume
512pub_type
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