Abstract:
:Missing contact footprinting with formic acid as a modifying reagent was used to examine specific IE-1 binding contacts to double-stranded oligonucleotides that contained either a consensus hr repeat sequence or a sequence from the pe38 promoter, which is down regulated by IE-1. The hr repeat sequences contain two consensus IE-1 binding motifs (IBMs) flanking a central EcoRI site that are oriented in opposite directions with respect to each other. IE-1 was found to contact regions including both IBMs. The bases footprinted in the top strand included the left IBM (IBM-A), whereas bases in the bottom strand were footprinted in a region that included IBM-B and part of IBM-A. When substitution mutations were introduced into either IBM, bases on both strands of the remaining IBM were strongly footprinted. As with the hr IBM-mutant constructs, bases footprinted in the pe38 promoter construct included both strands of the single IBM.
journal_name
Virologyjournal_title
Virologyauthors
Leisy DJ,Rohrmann GFdoi
10.1006/viro.2000.0452subject
Has Abstractpub_date
2000-08-15 00:00:00pages
196-202issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(00)90452-5journal_volume
274pub_type
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