Differential subcellular localization of hepatitis C virus core gene products.

abstract：:Influenza antiviral drugs that use protein inhibitors can lose their efficacy as resistant strains emerge. As an alternative strategy, we investigated the use of small interfering RNA molecules (siRNAs) by characterizing three siRNAs (M747, M776 and M832) targeting the influenza matrix 2 gene and three (NS570, NS595 a...

journal_title：Virology

authors： McMillen CM,Beezhold DH,Blachere FM,Othumpangat S,Kashon ML,Noti JD

更新日期：2016-10-01 00:00:00

abstract：:In vitro, the coat protein of brome mosaic virus (BMV) inhibited RNA synthesis by replicase extracted from BMV-infected barley leaves. The inhibition was due to the interaction of coat protein with BMV RNA. Under the same conditions, no inhibition by TMV coat protein or bovine serum albumin was detected. For the compl...

journal_title：Virology

authors： Horikoshi M,Nakayama M,Yamaoka N,Furusawa I,Shishiyama J

更新日期：1987-05-01 00:00:00

abstract：:As porcine endogenous retroviruses (PERV) productively infect human cells in vitro, they pose a serious risk in xenotransplantation and xenogeneic cell therapies. We have analyzed the prevalence of six well-characterized full-length PERV, five of them being replication-competent and four of them being chromosomally as...

journal_title：Virology

authors： Niebert M,Tönjes RR

更新日期：2003-09-01 00:00:00

abstract：:Hepatitis delta virus (HDV) encodes only one protein, the hepatitis delta antigen (HDAg). Two forms of HDAg, a large (27 kDa) and a small (24 kDa) one, participate in the various steps of HDV replication. To further understand the properties of HDAg, we have constructed recombinant baculoviruses and expressed both for...

journal_title：Virology

authors： Hwang SB,Lee CZ,Lai MM

更新日期：1992-09-01 00:00:00

abstract：:The sequence of the 5'-end of the genome of dengue 2 (Jamaica genotype) virus has been previously reported (V. Deubel, R. M. Kinney, and D. W. Trent, 1986, Virology 155, 365-377). We have now cloned and sequenced the remaining 75% of the genomic RNA that encodes the nonstructural proteins. The complete genome is 10,72...

journal_title：Virology

authors： Deubel V,Kinney RM,Trent DW

更新日期：1988-07-01 00:00:00

abstract：:Analogous to transmission of human T-cell leukemia virus type 1 (HTLV-1) in vivo, an in vitro cell-to-cell infection model was established by coculturing MT-2 cells as virus donors and HUT78 cells as recipients. At a donor:recipient ratio of 1:2, cell fusion occurred and a new round of HTLV-1 genome replication was in...

journal_title：Virology

authors： Benovic S,Kok T,Stephenson A,McInnes J,Burrell C,Li P

更新日期：1998-05-10 00:00:00

abstract：:The poxvirus virion contains an inner tubular nucleocapsid structure. The nucleocapsid is apparently labile to conventional electron microscopy fixation procedures and has therefore been largely ignored for decades. Advancements in electron microscopy sample preparation, notably high pressure freezing, better preserve...

journal_title：Virology

authors： McFadden BD,Moussatche N,Kelley K,Kang BH,Condit RC

更新日期：2012-12-05 00:00:00

abstract：:Viruses in certain genera in family Caulimoviridae were shown to integrate their genomic sequences into their host genomes and exist as endogenous pararetroviral sequences (EPRV). However, members of the genus Caulimovirus remained to be the exception and are known to exist only as episomal elements in the infected ce...

journal_title：Virology

authors： Pahalawatta V,Druffel K,Pappu H

更新日期：2008-07-05 00:00:00

abstract：:Vaccinia virus DNA ligase has been expressed in Escherichia coli, purified, and biochemically characterized. The enzyme ligates double-stranded (ds) DNA substrates with either cohesive or blunt-end termini and the latter reaction is stimulated by PEG. Vaccinia virus DNA ligase can also ligate oligo(dT) when annealed t...

journal_title：Virology

authors： Odell M,Kerr SM,Smith GL

更新日期：1996-07-01 00:00:00

abstract：:Adeno-associated virus (AAV-2) replicates to high titers when host cells are coinfected with a helper virus. Here we analyzed the coinfection of AAV-2 and mouse adenovirus (MAV-1) in murine fibroblasts. We observed that AAV-2/MAV-1 coinfected NIH 3T3 cells produced approximately 10-40-fold less AAV-2 DNAse resistant p...

journal_title：Virology

authors： Bhrigu V,Trempe JP

更新日期：2009-07-20 00:00:00

abstract：:Corn lethal necrosis (CLN) is caused by the synergistic interaction between maize chlorotic mottle machlomovirus (MCMV) and any potyvirus which infects cereals. Interactions between MCMV and wheat streak mosaic rymovirus (WSMV) in N28Ht corn produced MCMV concentrations that averaged 3.3- to 11.2-fold higher in doubly...

journal_title：Virology

authors： Scheets K

更新日期：1998-03-01 00:00:00

abstract：:Powassan virus (POWV, Flaviviridae: Flavivirus) is the sole North American member of the tick-borne encephalitis complex and consists of two distinct lineages that are maintained in ecologically discrete enzootic transmission cycles. The underlying genetic mechanisms that lead to niche partitioning in arboviruses are ...

journal_title：Virology

authors： Brackney DE,Brown IK,Nofchissey RA,Fitzpatrick KA,Ebel GD

更新日期：2010-07-05 00:00:00

abstract：:The genome of the sigma rhabdovirus of Drosophila melanogaster consists of six genes in the order 3' N-2-3-4-G-L 5'. The nucleotide sequences of the N and of the fourth genes were determined from cDNA clones. Each gene contained a single long open reading frame encoding polypeptides with predicted MW of 50 and 25 kDa,...

journal_title：Virology

authors： Bras F,Teninges D,Dezelee S

更新日期：1994-04-01 00:00:00

abstract：:The function of the 16-kDa protein encoded by tobacco rattle virus (TRV) RNA-1 was investigated by a mutational analysis of the 16-kDa protein gene. Transcripts of TRV RNA-1 produced from a full-length cDNA clone of TRV RNA-1 (SYM strain) remained infectious when the 16-kDa protein gene was disrupted by premature term...

journal_title：Virology

authors： Guilford PJ,Ziegler-Graff V,Baulcombe DC

更新日期：1991-06-01 00:00:00

abstract：:To elucidate the virulence factors of equine herpesvirus-1 (EHV-1), we compared the genomic architecture of the HH1 strain, a Japanese isolate of EHV-1, with that of its four descendant strains obtained by serial passage in bovine kidney (BK) cells, i.e. BK77, BK161, BK271, and BK343. In preliminary studies high-passa...

journal_title：Virology

authors： Kirisawa R,Ui S,Takahashi A,Kawakami Y,Iwai H

更新日期：1994-05-01 00:00:00

abstract：:The red clover necrotic mosaic dianthovirus (RCNMV) genome is split between two single-stranded RNA species termed RNA-1 and RNA-2. RNA-2 is required for infection of whole plants but is dispensable for infection and virion formation in protoplasts. We have used full-length cDNA clones of RNA-1 and -2 from which infec...

journal_title：Virology

authors： Xiong Z,Kim KH,Giesman-Cookmeyer D,Lommel SA

更新日期：1993-01-01 00:00:00

abstract：:Alphaherpesviruses comprise closely related viruses of man and animal, including herpes simplex virus, varicella-zoster virus and pseudorabies virus (PRV). Here, using methyl-beta-cyclodextrin and fluorescently tagged PRV, we directly show that depletion of cholesterol from the plasma membrane of host cells significan...

journal_title：Virology

authors： Desplanques AS,Nauwynck HJ,Vercauteren D,Geens T,Favoreel HW

更新日期：2008-07-05 00:00:00

abstract：:Cellular organelles of cauliflower mosaic virus-infected turnip plants were isolated, incubated with 32P-labeled deoxyribonucleoside triphosphates and assayed for their ability to synthesize virus-specific DNA. Only nuclei were found to synthesize a labeled DNA product. This DNA migrated during agarose gel electrophor...

journal_title：Virology

authors： Ansa OA,Bowyer JW,Shepherd RJ

更新日期：1982-08-01 00:00:00

abstract：:Ten mutations were introduced into the JC virus (JCV) T antigen within a region corresponding to the SV40 T-antigen DNA binding domain (SV40 amino acids 131 to 220); nine of these increased homology between the two proteins in sequences critical for SV40 T antigen DNA binding. All mutant JCV T antigens bound to JCV an...

journal_title：Virology

authors： Tavis JE,Frisque RJ

更新日期：1991-07-01 00:00:00

abstract：:Seven structural component proteins of a paramyxovirus simian virus 41 (SV41) were identified with the aid of monoclonal antibodies prepared against SV41 and human parainfluenza type 2 virus (PIV2). The nucleoprotein is antigenically very close to that of PIV2, while it is comparatively far from that of simian virus 5...

journal_title：Virology

authors： Tsurudome M,Bando H,Nishio M,Iwamoto Y,Kawano M,Kondo K,Komada H,Ito Y

更新日期：1990-12-01 00:00:00

abstract：:The X protein (pX) of hepatitis B virus (HBV) is a general transcription regulator and directly associated with the transcription machinery. pX cannot bind DNA directly but interacts with cellular factors that bind the regulatory elements. There is an accumulation of evidence concerning different activities exerted by...

journal_title：Virology

authors： de-Medina T,Haviv I,Noiman S,Shaul Y

更新日期：1994-07-01 00:00:00

abstract：:Camelpox virus (CMLV), a member of the Orthopoxvirus genus in the Poxviridae, is the etiologic agent of a disease of camels. Here we report the CMLV genomic sequence with analysis. The 205,719-bp CMLV genome contains 211 putative genes and consists of a central region bound by identical inverted terminal repeats of ap...

journal_title：Virology

authors： Afonso CL,Tulman ER,Lu Z,Zsak L,Sandybaev NT,Kerembekova UZ,Zaitsev VL,Kutish GF,Rock DL

更新日期：2002-03-30 00:00:00

abstract：:The envelope (E) protein of dengue virus (DENV) is composed of three domains (EDI, EDII, EDIII) and is the main target of neutralizing antibodies. Many monoclonal antibodies that bind EDIII strongly neutralize DENV. However in vitro studies indicate that anti-EDIII antibodies contribute little to the neutralizing pote...

journal_title：Virology

authors： Williams KL,Wahala WM,Orozco S,de Silva AM,Harris E

更新日期：2012-07-20 00:00:00

abstract：:To better understand the origin of human T-cell leukemia virus type l (HTLV-l) in South America, we conducted a phylogenetic study on 27 new HTLV-ls in Brazil. These were obtained from Brazilians of various ethnic origins, such as Japanese immigrants, whites, blacks and mulattos. We amplified and sequenced proviral DN...

journal_title：Virology

authors： Yamashita M,Veronesi R,Menna-Barreto M,Harrington WJ Jr,Sampio C,Brites C,Badaro R,Andrade-Filho AS,Okhura S,Igarashi T,Takehisa J,Miura T,Chamone D,Bianchini O,Jardim C,Sonoda S,Hayami M

更新日期：1999-08-15 00:00:00

abstract：:HCV genotype is a major determinant of clinical outcome, and GT1b HCV infection is the most difficult to treat and also the predominant genotype in East Asia and Europe. We developed 1b/JFH-1 inter-genotypic recombinants containing the structural genes (Core, E1, E2), p7 and the 1stTMD of NS2 directly from GT1b clinic...

journal_title：Virology

authors： Lu J,Tao W,Li R,Xiang Y,Zhang N,Xiang X,Xie Q,Zhong J

更新日期：2013-08-15 00:00:00

abstract：:Sequence heterogeneity of hepatitis B virus (HBV) is increasingly recognized to play a role in virus-host interaction. We have used a recently established method for HBV full-length genome amplification to search for novel types of HBV variants and to investigate further the sequence heterogeneity of HBV genome popula...

journal_title：Virology

authors： Sommer G,Günther S,Sterneck M,Otto S,Will H

更新日期：1997-12-22 00:00:00

abstract：:Tetherin has been defined as a restriction factor of HIV-1 and several other enveloped viruses. However, the significance of tetherin in viral infection remains to be further addressed. Here, we investigated whether tetherin plays a role in HSV-2 infection. Our study revealed that overexpression of tetherin restricted...

journal_title：Virology

authors： Liu Y,Luo S,He S,Zhang M,Wang P,Li C,Huang W,Hu B,Griffin GE,Shattock RJ,Hu Q

更新日期：2015-01-15 00:00:00

abstract：:In this study we have used an expression system based on Semliki Forest virus (SFV) to study assembly and intracellular localization of certain capsid proteins of rotavirus in neurons and mammalian epithelial cells. The complete genes of vp2 (vp2A) and vp6 (vp6A) of group A rotavirus (SA-11) and gene 5 encoding vp6 (v...

journal_title：Virology

authors： Nilsson M,von Bonsdorff CH,Weclewicz K,Cohen J,Svensson L

更新日期：1998-03-15 00:00:00

abstract：:RNA analysis by nondenaturing polyacrylamide gel electrophoresis and Northern blot hybridization of avocado chloroplasts purified from protoplasts of leaves infected by avocado sunblotch viroid (ASBVd) revealed the main ASBVd-specific bands found previously in preparations of total leaf RNA: the monomeric, dimeric and...

journal_title：Virology

authors： Navarro JA,Darós JA,Flores R

更新日期：1999-01-05 00:00:00

abstract：:Marek's disease (MD) infection has been controlled effectively by vaccination using nononcogenic and/or attenuated oncogenic Marek's disease virus (MDV) vaccines. Thus far, there is little knowledge on the role of cell-mediated immune (CMI) responses during MDV infection or vaccination. To elucidate the importance of ...

journal_title：Virology

authors： Omar AR,Schat KA

更新日期：1996-08-01 00:00:00