Abstract:
:The expression and subcellular location of the 33.6-kDa nonstructural protein NSm of tomato spotted wilt virus (TSWV) was analyzed in Nicotiana rustica plants and protoplasts as a function of time. Immunofluorescent studies in protoplasts isolated from TSWV-infected N. rustica leaves showed that this protein could first be detected close to the periphery of the cell, near the plasmamembrane, and later in tubular structures emerging from the cell surface. In situ, these tubules appeared specifically in the plasmodesmata, suggesting their involvement in cell-to-cell movement of the virus during systemic infection. In protoplasts transfected with an expression vector containing the NSm gene, similar tubules were formed, indicating that NSm has the ability to form these structures in the absence of other virus-specific components. To test whether plant-specific components were involved in tubule formation, the NSm gene was also expressed in a heterologous expression system, i.e., insect cells. Spodoptera frugiperda and Trichoplusia ni cells were infected with a recombinant baculovirus expressing the NSm-gene (AcNPV/NSm). The efficient formation of NSm-containing tubules emerging from the surface of both cell types indicate that no plant-specific cell structures or proteins are involved in their development.
journal_name
Virologyjournal_title
Virologyauthors
Storms MM,Kormelink R,Peters D,Van Lent JW,Goldbach RWdoi
10.1006/viro.1995.0059subject
Has Abstractpub_date
1995-12-20 00:00:00pages
485-93issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(85)70059-1journal_volume
214pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Marek's disease (MD) infection has been controlled effectively by vaccination using nononcogenic and/or attenuated oncogenic Marek's disease virus (MDV) vaccines. Thus far, there is little knowledge on the role of cell-mediated immune (CMI) responses during MDV infection or vaccination. To elucidate the importance of ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0400
更新日期:1996-08-01 00:00:00
abstract::The uncoating of tobacco mosaic virus (TMV) particles in protoplasts isolated from leaves of transgenic tobacco plants that express the TMV coat protein gene was investigated. Extracts of these protoplasts collected up to 1 hr after inoculation with TMV contained fewer of the complexes ("striposomes") thought to be in...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90163-l
更新日期:1990-12-01 00:00:00
abstract::Altered cytokine profiles and imbalanced frequencies of CD4(+) T helper cell subsets are thought to be linked with HIV-1/AIDS pathogenesis, but the causes need to be further clarified. Histamine, a biogenic amine with many functions, shows enhancement in HIV-1 infected individuals, which are considered to link with di...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.04.009
更新日期:2013-08-01 00:00:00
abstract::A novel human papillomavirus type (HPV) was cloned from an invasive cervical carcinoma. The viral clone showed no homology with other known prototypes of HPV (HPV-1 through HPV-57), except HPV-33 by Southern blot analysis under stringent conditions. It showed less than 20% homology to HPV-33 by reassociation kinetic a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90560-e
更新日期:1990-08-01 00:00:00
abstract::Repression of human papillomavirus (HPV) E6 and E7 oncogenes in established cervical carcinoma cell lines causes senescence due to reactivation of cellular tumor suppressor pathways. Here, we determined whether ongoing expression of HPV16 or HPV18 oncogenes is required for the proliferation of primary human cervical c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.10.012
更新日期:2012-01-05 00:00:00
abstract::Most adenovirus-specific mRNAs expressed late after infection originate from a single transcription unit, the so-called major late transcription unit. All mRNAs expressed from this unit have in common a 201 nucleotide-long spliced tripartite leader segment attached to their 5' ends. Human adenovirus mutants that carry...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1234
更新日期:1993-05-01 00:00:00
abstract::The human hepatitis B Virus genome (HBV) contains a liver-specific enhancer upstream of the X ORF which has been studied in detail by several investigators. A second liver-specific enhancer element, designated here as enhancer II, has been relatively recently described in the HBV genome, which is located within the co...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)91019-d
更新日期:1991-08-01 00:00:00
abstract::While several studies have shown a role for autophagy in the replication of dengue virus (DENV), these studies have been performed in directly infected cells. However, in severe cases of DENV infection the critical cell in the disease is believed to be monocytes which are poorly infected directly, but are highly susce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.07.010
更新日期:2011-09-15 00:00:00
abstract::Intact clones containing partial repeats of the genomic components of African cassava mosaic (ACMV DNAs A and B) are infectious when mechanically coinoculated onto Nicotiana benthamiana. Monomeric genomic components may be generated either by homologous recombination or, when two copies of the origin of replication (o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(95)80093-x
更新日期:1995-01-10 00:00:00
abstract::In retrovirus-induced tumors, proviral DNA is commonly found next to or within a cellular proto-oncogene such that transcription of the gene is influenced by the viral promoter or enhancer. Extensive surveys of naturally occurring tumors reveal that proviruses integrated on the 3' side of the gene are usually in the s...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1245
更新日期:1993-05-01 00:00:00
abstract::RNA analysis by nondenaturing polyacrylamide gel electrophoresis and Northern blot hybridization of avocado chloroplasts purified from protoplasts of leaves infected by avocado sunblotch viroid (ASBVd) revealed the main ASBVd-specific bands found previously in preparations of total leaf RNA: the monomeric, dimeric and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9497
更新日期:1999-01-05 00:00:00
abstract::We assess the genetic relationships between 49 HIV-1 group O strains from 24 and 25 patients living in Cameroon and France, respectively. Strains were sequenced in four genomic regions: gag (p24) and three env regions (C2-V3, gp41, and for 22 C2-gp41). In each of the genomic regions analyzed, the genetic diversity amo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1430
更新日期:2002-10-25 00:00:00
abstract::As porcine endogenous retroviruses (PERV) productively infect human cells in vitro, they pose a serious risk in xenotransplantation and xenogeneic cell therapies. We have analyzed the prevalence of six well-characterized full-length PERV, five of them being replication-competent and four of them being chromosomally as...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00316-7
更新日期:2003-09-01 00:00:00
abstract::Rat 3Y1 cells were infected by AKR virus through microinjection of molecularly cloned proviral DNA. Based on a strong immunofluorescence using anti-p30 as an antiserum a cell clone (RESA-2) was selected that had a high expression of viral antigens. Subsequent restriction analysis of its DNA revealed that the RESA-2 cl...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90361-6
更新日期:1983-01-30 00:00:00
abstract::We have utilized glycan microarray technology to determine the receptor binding properties of early isolates from the recent 2009 H1N1 human pandemic (pdmH1N1), and compared them to North American swine influenza isolates from the same year, as well as past seasonal H1N1 human isolates. We showed that the pdmH1N1 stra...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.01.027
更新日期:2011-05-10 00:00:00
abstract::Attenuated mutants of Venezuelan equine encephalitis virus (VEE) were isolated by selection for rapid penetration of cultured cells (R. E. Johnston and J. F. Smith, 1988, Virology 162, 437-443). Sequence analysis of these mutants identified candidate attenuating mutations at four loci in the VEE E2 glycoprotein gene: ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90114-q
更新日期:1991-07-01 00:00:00
abstract::Several sulfated polysaccharides (dextran sulfate, pentosan polysulfate, heparin) and copolymers of acrylic acid with vinylalcohol sulfate have proved to be potent inhibitors of human cytomegalovirus (CMV) infectivity in vitro. Sulfated alpha-cyclodextrins are only weak inhibitors of CMV. A close correlation was found...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90680-n
更新日期:1992-07-01 00:00:00
abstract::The envelope glycoprotein E2 of hepatitis C virus (HCV) is a major component of the viral envelope. Knowledge of its topologic features and antigenic determinants in virions is crucial in understanding the viral binding sites to cellular receptor(s) and the induction of neutralizing antibodies. The lack of a robust ce...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1463
更新日期:2002-06-20 00:00:00
abstract::We have examined FIV vif function in the context of molecular clone pF34 (GenBank Accession No. M25729). Rabbit antisera directed against the translation product of the vif gene identified a 29-kDa protein in tissue culture cells infected with FIV-pF34; this protein was not present in cultures of uninfected cells. Thu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1609
更新日期:1994-11-01 00:00:00
abstract::Human cytomegalovirus (HCMV) glycoprotein B (gB) promotes virion entry into cells by fusing the virion envelope with the cellular membrane. We recently reported that UB cells (U373 glioblastoma cells constructed to produce HCMV gB constitutively) form multinucleate syncytia that are dependent on the density of gB in t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1290
更新日期:1995-06-01 00:00:00
abstract::Puumala (PUUV) and Hantaan (HTNV) viruses are hantaviruses within the family Bunyaviridae and associated with Hemorrhagic Fever with Renal Syndrome (HFRS) in humans. Little is known about how these viruses interact with host cells, though pathogenic hantaviruses interact with α(v)β(3) integrin. To study host cell inte...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.08.012
更新日期:2012-02-20 00:00:00
abstract::The primary cell types that sustain polyomavirus (Py) replication following intranasal infection as well as the nature of the host cellular response to Py were unknown. As this is an essential and specific site for virus entry, it seems likely that viral gene function must be adapted to these mucosal tissues. Using im...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0030
更新日期:2000-01-05 00:00:00
abstract::To help elucidate the mechanisms by which retrovirus structural proteins associate to form virus particles, we have examined membrane-bound assemblies of Moloney murine leukemia virus (M-MuLV) capsid (CA) proteins. Electron microscopy and image reconstruction techniques showed that CA dimers appear to function as orga...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1452
更新日期:2002-06-20 00:00:00
abstract::The International Human Papillomavirus (HPV) Reference Center supports quality and order in HPV research and diagnostics. Notably, the center assigns HPV type numbers to novel HPV types, maintains a reference clone repository, and issues international proficiency panels for HPV genotyping. The established HPV types, c...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2018.04.003
更新日期:2018-06-01 00:00:00
abstract::Ribavirin is the first synthetic, broad-spectrum antiviral nucleoside. Despite its more than 30 year history, the mechanism of action of this compound remains unclear and somewhat controversial. Recent data suggest the possibility that the activity of ribavirin against RNA viruses is a reflection of incorporation of r...
journal_title:Virology
pub_type: 杂志文章,评审
doi:10.1006/viro.2002.1487
更新日期:2002-07-05 00:00:00
abstract::The sequence of the 5'-end of the genome of dengue 2 (Jamaica genotype) virus has been previously reported (V. Deubel, R. M. Kinney, and D. W. Trent, 1986, Virology 155, 365-377). We have now cloned and sequenced the remaining 75% of the genomic RNA that encodes the nonstructural proteins. The complete genome is 10,72...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90677-0
更新日期:1988-07-01 00:00:00
abstract::Studies of the cellular autophagy pathway have exploded over the past twenty years. Now appreciated as a constitutive degradative mechanism that promotes cellular homeostasis, autophagy is also required for a variety of developmental processes, cellular stress responses, and immune pathways. Autophagy certainly acts a...
journal_title:Virology
pub_type: 杂志文章,评审
doi:10.1016/j.virol.2015.03.042
更新日期:2015-05-01 00:00:00
abstract::Recombinants of Moloney murine leukemia virus (MoMuLV) with either an amphotropic (MoAmphoV) or 10A1-tropic host range (Mo10A1V) induce a spongiform neurodegenerative disease in susceptible mice. To test whether MoMuLV -derived sequences are required for induction of neuropathology, mice were inoculated with either th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00210-1
更新日期:2003-08-15 00:00:00
abstract::A mutant (Tbts7) of alfalfa mosaic virus, the coat protein of which is unable to activate the viral genome (the RNA species 1, 2, and 3, which need some coat protein for infectivity) at 30 degrees , can be rescued at this temperature by adding to the inoculum wild-type RNA 3 (the genome part that contains the coat pro...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(82)90528-1
更新日期:1982-02-01 00:00:00
abstract::A collection of randomly generated temperature-sensitive (ts) vaccinia virus (strain IHD-W) mutants were reported by S. Dales et al., (1978, Virology, 84, 403-428) in 1978 and characterized by electron microscopy. We have performed further genetic analysis on the Dales collection of mutants to make the mutants more us...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1745
更新日期:2003-01-20 00:00:00