Quasispecies, error catastrophe, and the antiviral activity of ribavirin.

abstract：:To clarify the causal relationship between human papillomavirus (HPV) and cervical intraepithelial neoplasia (CIN), we analyzed 386 unfixed biopsy specimens by blot hybridization at Tm -40 degrees C, targeting 38 skin and 42 genital HPVs. By the recognition of PstI, BanI, and MspI cleavage patterns, single genital, bu...

journal_title：Virology

authors： Matsukura T,Sugase M

更新日期：2001-04-25 00:00:00

abstract：:In this report, we examined the abilities and requirements of heterologous Gag proteins to functionally complement each other to support viral replication. Two distantly related gammaretroviruses, murine leukemia virus (MLV) and spleen necrosis virus (SNV), were used as a model system because SNV proteins can support ...

journal_title：Virology

authors： Lee SK,Boyko V,Hu WS

更新日期：2007-04-10 00:00:00

abstract：:Two temperature-sensitive mutants of herpes simplex virus type 1 in complementation group 1-1 were analyzed to determine if the major DNA-binding protein they produced was thermolabile. Cells infected with these mutants were analyzed for deoxyribonucleoprotein complexes containing the DNA-binding protein. These comple...

journal_title：Virology

authors： Leinbach SS,Casto JF,Pickett TK

更新日期：1984-09-01 00:00:00

abstract：:High levels of nonfused chloramphenicol acetyltransferase, beta-galactosidase, and beta-glucuronidase expressed under the control of new vector constructs of the polyhedrin promoter in Spodoptera frugiperda cells infected with Autographa californica nuclear polyhedrosis virus were investigated by SDS-PAGE and RNA dot ...

journal_title：Virology

authors： Luckow VA,Summers MD

更新日期：1989-05-01 00:00:00

abstract：:Recently, cases of severe respiratory illness in military and civilian populations have been associated with a new genomic variant of adenovirus (Ad) serotype 14, designated Ad14a. Compared to the Ad14 reference strain (de Wit), this new virus had a deletion of two amino acid residues in the fiber protein knob. Here w...

journal_title：Virology

authors： Wang H,Tuve S,Erdman DD,Lieber A

更新日期：2009-05-10 00:00:00

abstract：:The prototypic macrophage-tropic HIV-1 isolate, HIV-1BaL, cannot replicate in the monocytoid cell line THP-1. After induction of differentiation by a phorbol diester, a fraction of THP-1 cells became permissive to HIV-1BaL. In contrast, this treatment decreased permissiveness for the lymphotropic isolate HIV-1LAI. Vir...

journal_title：Virology

authors： Meylan PR,Spina CA,Richman DD,Kornbluth RS

更新日期：1993-03-01 00:00:00

abstract：:In order to define factors involved in very late Autographa californica nucleopolyhedrovirus (AcMNPV) gene function, random mutagenesis of a baculovirus recombinant (AcUW1.lacZ) by 5'-bromodeoxyuridine treatment was performed. Five viruses were selected with deficiencies in very late gene expression. These were charac...

journal_title：Virology

authors： Merrington CL,Kitts PA,King LA,Possee RD

更新日期：1996-03-01 00:00:00

abstract：:Maturation of the arenavirus GP precursor (GPC) involves proteolytic processing by cellular signal peptidase and the proprotein convertase subtilisin kexin isozyme 1 (SKI-1)/site 1 protease (S1P), yielding a tripartite complex comprised of a stable signal peptide (SSP), the receptor-binding GP1, and the fusion-active ...

journal_title：Virology

authors： Burri DJ,Pasquato A,da Palma JR,Igonet S,Oldstone MB,Kunz S

更新日期：2013-02-05 00:00:00

abstract：:We have determined the nucleotide sequence of the RNA2 segment of the Nudaurelia capensis omega virus genome. It was found to consist of 2448 nucleotides and contained one long open reading frame (ORF) encoding the 644 residue capsid protein. The deduced amino acid sequence of this protein reveals a positively charged...

journal_title：Virology

authors： Agrawal DK,Johnson JE

更新日期：1992-10-01 00:00:00

abstract：:One of the newest niches for baculoviruses-based technologies is their use as vectors for mammalian cell transduction and gene therapy applications. However, an outstanding safety issue related to such use is the residual expression of viral genes in infected mammalian cells. Here we show that infectious baculoviruses...

journal_title：Virology

authors： Efrose R,Swevers L,Iatrou K

更新日期：2010-10-25 00:00:00

abstract：:Herpesvirus glycoprotein C (gC) functions as a major virus attachment protein. The gC sequence of the neurovirulent bovine herpesvirus type 5 (BHV-5) virus was determined and compared with the gC sequence of the nonneurovirulent BHV-1. Alignment of the predicted amino acid sequences of BHV-1 and BHV-5 gC ORFs showed t...

journal_title：Virology

authors： Chowdhury SI

更新日期：1995-11-10 00:00:00

abstract：:Bacteriophage T4 tails contain a set of extended protein molecules in the central channel of the tail tube through which the DNA must exit during infection. Treatment of tails with guanidine hydrochloride separates the baseplates, leaving the tail tube and several specific tube-associated proteins. Methods were develo...

journal_title：Virology

authors： Duda RL,Gingery M,Eiserling FA

更新日期：1986-06-01 00:00:00

abstract：:CD8 T cells play an important role in controlling Ebola infection and in mediating vaccine-induced protective immunity, yet little is known about antigenic targets in Ebola that are recognized by CD8 T cells. Overlapping peptides were used to identify major histocompatibility complex class I-restricted epitopes in mic...

journal_title：Virology

authors： Simmons G,Lee A,Rennekamp AJ,Fan X,Bates P,Shen H

更新日期：2004-01-05 00:00:00

abstract：:The Autographa californica nuclear polyhedrosis virus da26 gene codes for an envelope protein of both budded virus (BV) and occlusion derived virus (ODV). Western blot and temporal analysis of infected cell extracts detected a protein of 26 kDa by 4 h postinfection (p.i.). The amount of protein increased by 16 h p.i. ...

journal_title：Virology

authors： Beniya H,Braunagel SC,Summers MD

更新日期：1998-01-05 00:00:00

abstract：:The 3' end of hepatitis E virus (HEV) contains cis-acting regulatory element, which plays an important role in viral replication. To develop specific replication inhibitor at the molecular level, mono- and di-hammerhead ribozymes (Rz) were designed and synthesized against the conserved 3' end sequences of HEV, which c...

journal_title：Virology

authors： Sriram B,Thakral D,Panda SK

更新日期：2003-08-01 00:00:00

abstract：:Hepatitis B viruses replicate via reverse transcription of an RNA intermediate. This RNA pregenome serves as mRNA and is packaged into capsids and reverse transcribed. Both processes require the interaction of the viral reverse transcriptase, P protein, with the 5'-proximal epsilon-signals on the pregenome. For epsilo...

journal_title：Virology

authors： Beck J,Bartos H,Nassal M

更新日期：1997-01-20 00:00:00

abstract：:Natural antibodies to gp41 inhibit HIV-1 replication through the recognition of two different regions, corresponding to the leucine zipper motif in the HR1 alpha-helix and to another motif within HR2 region, hosting 2F5 and 4E10 epitope. This study aimed at reproducing such protective responses through VLP vaccination...

journal_title：Virology

authors： Pastori C,Tudor D,Diomede L,Drillet AS,Jegerlehner A,Röhn TA,Bomsel M,Lopalco L

更新日期：2012-09-15 00:00:00

abstract：:Equid herpesvirus-1 (EHV-1) outbreaks continue despite widely used vaccination. We demonstrated previously that an ORF1/ORF71 gene deletion mutant of the EHV-1 strain Ab4 (Ab4ΔORF1/71) is less virulent than its parent Ab4 virus. Here, we describe the Ab4 challenge infection evaluating protection induced by the Ab4ΔORF...

journal_title：Virology

authors： Perkins G,Babasyan S,Stout AE,Freer H,Rollins A,Wimer CL,Wagner B

更新日期：2019-05-01 00:00:00

abstract：:Endogenous feline leukemia viruses (enFeLVs) occur in the germ lines of the domestic cat and related wild species (genus Felis). We sequenced the long terminal repeats and part of the env region of enFeLVs in domestic cats and five wild species. A total of 305 enFeLV sequences were generated across 17 individuals, dem...

journal_title：Virology

authors： Polani S,Roca AL,Rosensteel BB,Kolokotronis SO,Bar-Gal GK

更新日期：2010-09-30 00:00:00

abstract：:Commelina yellow mottle virus (CoYMV) is the type member of the badnaviruses, a genus of plant pararetroviruses. The N-terminus of the polyprotein encoded by ORF III has limited similarity to known cell-to-cell movement proteins. To test the hypothesis that the N-terminus is required for viral movement, the phenotypes...

journal_title：Virology

authors： Tzafrir I,Ayala-Navarrete L,Lockhart BE,Olszewski NE

更新日期：1997-06-09 00:00:00

abstract：:The main route of small ruminant lentivirus dissemination is the ingestion of infected cells present in colostrum and milk from infected animals. However, whether only macrophages or other cell subtypes are involved in this transmission is unknown. We derived epithelial cell cultures, 100% cytokeratin positive, from m...

journal_title：Virology

authors： Mselli-Lakhal L,Guiguen F,Fornazero C,Du J,Favier C,Durand J,Grezel D,Balleydier S,Mornex JF,Chebloune Y

更新日期：1999-06-20 00:00:00

abstract：:Infection of lymphocytes by the Epstein-Barr virus (EBV) is initiated by attachment of the major virus glycoprotein gp350/220 to a cell surface glycoprotein, known as CR2 (CD21). In a productive infection the virus envelope fuses with host cell membranes either at the cell surface or within endocytic vesicles. To inve...

journal_title：Virology

authors： Patel RA,Hutt-Fletcher LM,Crews FT

更新日期：1993-07-01 00:00:00

abstract：:Potato spindle tuber viroid (PSTVd) is a small non-protein-coding RNA pathogen that can induce disease symptoms in a variety of plant species. How PSTVd induces disease symptoms is a long standing question. It has been suggested that PSTVd-derived small RNAs (sRNAs) could direct RNA silencing of a targeted host gene(s...

journal_title：Virology

authors： Eamens AL,Smith NA,Dennis ES,Wassenegger M,Wang MB

更新日期：2014-02-01 00:00:00

abstract：:The major capsid protein of HPV, L1, assembles into pentamers that form a T = 7 icosahedral particle, but the location of the co-assembled minor capsid protein, L2, remains controversial. Several researchers have developed useful monoclonal antibodies targeting L2, but most react with linear epitopes toward the N-term...

journal_title：Virology

authors： Bywaters SM,Brendle SA,Biryukov J,Wang JW,Walston J,Milici J,Roden RB,Meyers C,Christensen ND

更新日期：2018-11-01 00:00:00

abstract：:Coronavirus (CoV) envelope (E) protein ion channel activity was determined in channels formed in planar lipid bilayers by peptides representing either the transmembrane domain of severe acute respiratory syndrome CoV (SARS-CoV) E protein, or the full-length E protein. Both of them formed a voltage independent ion cond...

journal_title：Virology

authors： Verdiá-Báguena C,Nieto-Torres JL,Alcaraz A,DeDiego ML,Torres J,Aguilella VM,Enjuanes L

更新日期：2012-10-25 00:00:00

abstract：:The single open reading frame (ORF) 5 encoding the 50-kDa protein of barley yellow dwarf virus PAV-IL (BYDV-PAV-IL) was expressed in bacteria, purified, and used as an immunogen/antigen to produce/screen antibodies specific to the 50-kDa protein. Two monoclonal antibodies (MAb PAV-IL-22 kDa and MAb PAV-IL-50 kDa) rais...

journal_title：Virology

authors： Cheng SL,Domier LL,D'Arcy CJ

更新日期：1994-08-01 00:00:00

abstract：:A human cytomegalovirus (HCMV)-induced polypeptide of 68,000 Da (p68) with protein kinase activity was identified using a monoclonal antibody (F6b) produced against HCMV-infected cell proteins. p68 was detected by immunoprecipitation from 3 to 120 hr after infection and was induced by several strains of human and not ...

journal_title：Virology

authors： Michelson S,Tardy-Panit M,Bârzu O

更新日期：1984-04-30 00:00:00

abstract：:Replication and transmission of avian influenza virus in humans poses a pandemic threat. The molecular determinants that facilitate this process are not well understood. We used DBA/2 mice to identify viral factors that mediate the difference in pathogenesis between a virulent (H7N3) and a non-virulent (H7N9) avian in...

journal_title：Virology

authors： DesRochers BL,Chen RE,Gounder AP,Pinto AK,Bricker T,Linton CN,Rogers CD,Williams GD,Webby RJ,Boon AC

更新日期：2016-07-01 00:00:00

abstract：:Six mutants of lambda having reduced dependence on the htpR function of Escherichia coli were isolated from lambda cIts857. Burst sizes in htpRts cells at 40.5 degrees were in the range of 10 to 20 particles per cell. Mapping and complementation analysis of one of the mutants suggested that the mutation in this isolat...

journal_title：Virology

authors： Fuerst CR

更新日期：1986-02-01 00:00:00

abstract：:We have used a yeast two-hybrid system to show that human papillomavirus E7 proteins can form oligomeric complexes in vivo. The carboxyl-terminal cysteine-rich metal-binding domain is critical for this activity although amino-terminal sequences also contribute to oligomerization. Our experiments also reveal that E7 po...

journal_title：Virology

authors： Clemens KE,Brent R,Gyuris J,Münger K

更新日期：1995-12-01 00:00:00