当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Efficient production of Hantaan and Puumala pseudovirions for viral tropism and neutralization studies.

Efficient production of Hantaan and Puumala pseudovirions for viral tropism and neutralization studies.

Abstract：

:Puumala (PUUV) and Hantaan (HTNV) viruses are hantaviruses within the family Bunyaviridae and associated with Hemorrhagic Fever with Renal Syndrome (HFRS) in humans. Little is known about how these viruses interact with host cells, though pathogenic hantaviruses interact with α(v)β(3) integrin. To study host cell interactions and rapidly test the ability of antibodies to prevent infection, we produced HTNV and PUUV pseudovirions on a vesicular stomatitis virus (VSV) core. Similar to replication-competent hantaviruses, infection was low-pH-dependent. Despite broad cell tropism, several human T cell lines were poorly permissive to hantavirus pseudovirions, compared to VSV, indicating a relative block to infection at the level of entry. Stable expression of α(v)β(3) integrin in SupT1 cells did not restore infectivity. Finally, the pseudovirion system provided a rapid, quantitative, and specific method to screen for neutralizing antibodies in immune sera.

journal_name

Virology

journal_title

Virology

authors

Higa MM,Petersen J,Hooper J,Doms RW

doi

10.1016/j.virol.2011.08.012

subject

Has Abstract

pub_date

2012-02-20 00:00:00

pages

134-42

issue

2

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(11)00375-8

journal_volume

423

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • The role of the AcMNPV 25K gene, "FP25," in baculovirus polh and p10 expression.

    abstract：:A previous study showed that an Autographa californica multicapsid nuclear polyhedrosis virus (AcMNPV) 25K mutant produced less polyhedrin protein than wild-type (Jarvis et al., J. Virol. 66, 6903-6911, 1992). In this study, the role of the 25K gene product (AcMNPV ORF 61) in baculovirus gene expression was further in...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1996.0625

    authors： Harrison RL,Jarvis DL,Summers MD

    更新日期：1996-12-01 00:00:00

  • The Rice stripe virus pc4 functions in movement and foliar necrosis expression in Nicotiana benthamiana.

    abstract：:The Rice stripe virus (RSV) pc4 has been determined as the viral movement protein (MP). In this study, the pc4 gene was cloned into a movement-deficient Tobacco mosaic virus (TMV). The resulting hybrid TMV-pc4, in addition to spreading cell to cell in Nicotiana tabacum, moved systemically and induced foliar necrosis i...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.01.007

    authors： Zhang C,Pei X,Wang Z,Jia S,Guo S,Zhang Y,Li W

    更新日期：2012-04-10 00:00:00

  • Virion-associated viral fibroblast growth factor stimulates cell motility.

    abstract：:The Autographa californica M nucleopolyhedrovirus (AcMNPV) viral fibroblast growth factor (vFGF) has functional parallels to cellular FGFs. Deletion of the AcMNPV vfgf has no obvious phenotype in cell culture but delays the time of insect death. Here, we determined vFGF production during virus infection. vFGF was dete...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.09.011

    authors： Lehiy CJ,Martinez O,Passarelli AL

    更新日期：2009-12-05 00:00:00

  • Functional expression of influenza A viral nucleoprotein in cells transformed with cloned DNA.

    abstract：:Simian cells permissive for influenza A virus infection were stably transformed with a full-length cloned influenza A nucleoprotein gene under the control of an inducible metallothionein promoter and linked to a dihydrofolate reductase gene to facilitate cell selection. Transformed cells synthesized a virus-specific n...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90437-x

    authors： Ryan KW,Mackow ER,Chanock RM,Lai CJ

    更新日期：1986-10-15 00:00:00

  • An insertion mutation in the pol gene of Moloney murine leukemia virus results in temperature-sensitive pol maturation and viral replication.

    abstract：:An insertion mutation in the pol gene of Moloney murine leukemia virus (M-MuLV) was found to render the virus temperature-sensitive for replication. A provirus containing a 12-bp insertion at the boundary between the reverse transcriptase (RT) and integrase (IN) domains induced the formation of mutant virions containi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90428-5

    authors： Tanese N,Roth M,Epstein H,Goff SP

    更新日期：1989-06-01 00:00:00

  • Cross-inhibition to heterologous foot-and-mouth disease virus infection induced by RNA interference targeting the conserved regions of viral genome.

    abstract：:RNA interference (RNAi) is the process by which double-stranded RNA (dsRNA) directs sequence-specific degradation of messenger RNA in animal and plant cells. In mammalian cells, RNAi can be triggered by 21-23 nucleotide duplexes of small interfering RNA (siRNA). Strategies to inhibit RNA virus multiplication based on ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.01.051

    authors： Liu M,Chen W,Ni Z,Yan W,Fei L,Jiao Y,Zhang J,Du Q,Wei X,Chen J,Liu Y,Zheng Z

    更新日期：2005-05-25 00:00:00

  • Identification and characterization of an equine herpesvirus 1 late gene encoding a potential zinc finger.

    abstract：:In this report, we present the DNA sequence and transcriptional characterization of a gene (IR5) that maps within each of the inverted repeat (IR) segments of the equine herpesvirus type 1 (EHV-1) genome. The IR5 open reading frame (ORF) is located within both IR sequences (nucleotides 9932-10,642 of the IR). DNA sequ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90525-t

    authors： Holden VR,Yalamanchili RR,Harty RN,O'Callaghan DJ

    更新日期：1992-06-01 00:00:00

  • No role for pepstatin-A-sensitive acidic proteinases in reovirus infections of L or MDCK cells.

    abstract：:Strong evidence indicates that virions of mammalian reoviruses undergo proteolytic processing by acid-dependent cellular proteinases as an essential step in productive infection. Proteolytic processing takes the form of a series of cleavages of outer-capsid proteins final sigma3 and mu1/mu1C. Previous studies showed a...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9434

    authors： Kothandaraman S,Hebert MC,Raines RT,Nibert ML

    更新日期：1998-11-25 00:00:00

  • Influenza virus polymerase confers independence of the cellular cap-binding factor eIF4E for viral mRNA translation.

    abstract：:The influenza virus mRNAs are structurally similar to cellular mRNAs nevertheless; the virus promotes selective translation of viral mRNAs despite the inhibition of host cell protein synthesis. The infection proceeds normally upon functional impairment of eIF4E cap-binding protein, but requires functional eIF4A helica...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.10.028

    authors： Yángüez E,Rodriguez P,Goodfellow I,Nieto A

    更新日期：2012-01-20 00:00:00

  • Adenovirus transformation revertant resistant to retransformation by E1 but not by SV40-T and HPV16-E7 oncogenes.

    abstract：:We have previously described a revertant cell line which expresses a dominant tumor suppressor phenotype to E1 but not to heterologous oncogenes such as c-myc, N-ras, or polyoma middle t (Sircar et al. (1988) Oncogene 3, 725-728). DNA tumor virus oncogenes have been suggested to transform cells via the common mechanis...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90180-w

    authors： Sircar S,Horvath J,Roberge D,Diouri M,Weber JM

    更新日期：1992-11-01 00:00:00

  • Identification of the 5' end of the rubella virus subgenomic RNA.

    abstract：:The 5' end of the subgenomic RNA of rubella virus was determined by primer extension. The Maxam-Gilbert sequence ladder of the primer extension product contained a determinable sequence which was colinear with the complement of the sequence of the genomic RNA through nucleotide 3325 from the 3' end of the genomic RNA ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90422-4

    authors： Frey TK,Marr LD,Sanchez A,Simmons RB

    更新日期：1989-01-01 00:00:00

  • Human T-cell leukemia virus type II: primary structure analysis of the major internal protein, p24 and the nucleic acid binding protein, p15.

    abstract：:The 24,000-molecular-weight major internal protein (p24) and the 15,000-molecular-weight nucleic acid binding protein (p15) of human T-cell leukemia virus type II (HTLV-II) were subjected to amino acid composition and amino-terminal amino acid sequence analysis. A comparison of amino acid composition of p24 and p15 of...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(85)90434-9

    authors： Devare SG,Kim Y,Fox JL,Getchell J,Cabradilla C,Kalyanaraman VS

    更新日期：1985-04-15 00:00:00

  • Binding of inferred germline precursors of broadly neutralizing HIV-1 antibodies to native-like envelope trimers.

    abstract：:HIV-1 envelope glycoproteins (Env) and Env-based immunogens usually do not interact efficiently with the inferred germline precursors of known broadly neutralizing antibodies (bNAbs). This deficiency may be one reason why Env and Env-based immunogens are not efficient at inducing bNAbs. We evaluated the binding of 15 ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2015.08.002

    authors： Sliepen K,Medina-Ramírez M,Yasmeen A,Moore JP,Klasse PJ,Sanders RW

    更新日期：2015-12-01 00:00:00

  • Structure and reversion of an adenovirus type 2 mutant containing a duplication of the left end of the genome at the right end.

    abstract：:A mutant (dp 201) of human adenovirus type 2 has been isolated that has the left 3% (1110 bp) of the genome duplicated at the right terminus. The structure of the mutant genome was studied by restriction endonuclease analysis and by direct DNA sequence analysis. The duplication has resulted in a deletion of 10 bp of t...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(83)90177-0

    authors： Brusca JS,Chinnadurai G

    更新日期：1983-09-01 00:00:00

  • Isolation and characterization of Nylanderia fulva virus 1, a positive-sense, single-stranded RNA virus infecting the tawny crazy ant, Nylanderia fulva.

    abstract：:We report the discovery of Nylanderia fulva virus 1 (NfV-1), the first virus identified and characterized from the ant, Nylanderia fulva. The NfV-1 genome (GenBank accession KX024775) is 10,881 nucleotides in length, encoding one large open reading frame (ORF). Helicase, protease, RNA-dependent RNA polymerase, and jel...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2016.06.014

    authors： Valles SM,Oi DH,Becnel JJ,Wetterer JK,LaPolla JS,Firth AE

    更新日期：2016-09-01 00:00:00

  • Virus neutralising activity of African fruit bat (Eidolon helvum) sera against emerging lyssaviruses.

    abstract：:It is likely that phylogroup 2 lyssaviruses circulate within bat reservoirs. We adapted a pseudotype (pt) neutralisation assay (PNA) to a multiplex format enabling serosurveillance for Lagos bat virus (LBV), Mokola virus (MOKV) and West Caucasian bat virus (WCBV) in a potential reservoir, the African straw-coloured fr...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.09.014

    authors： Wright E,Hayman DT,Vaughan A,Temperton NJ,Wood JL,Cunningham AA,Suu-Ire R,Weiss RA,Fooks AR

    更新日期：2010-12-20 00:00:00

  • Are the acid-labile interferon alpha and interferon omega-1 identical?

    abstract：:The interferon (IFN) activity found in human leukocyte IFN alpha preparations, autoimmune and AIDS sera, and others was reported to have distinct antigenic and deviating biological properties. This led to its vague designation as acid-labile and thermolabile IFN alpha. However, using specific monoclonal antibodies, th...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90517-f

    authors： Kontsek P,Borecký L,Novák M

    更新日期：1991-03-01 00:00:00

  • Changes in the intraisolate genetic structure of Beet necrotic yellow vein virus populations associated with plant resistance breakdown.

    abstract：:The causal agent of rhizomania disease, Beet necrotic yellow vein virus (BNYVV), typically produces asymptomatic root-limited infections in sugar beets (Beta vulgaris) carrying the Rz1-allele. Unfortunately, this dominant resistance has been recently overcome. Multiple cDNA clones of the viral pathogenic determinant p...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.03.008

    authors： Acosta-Leal R,Fawley MW,Rush CM

    更新日期：2008-06-20 00:00:00

  • Deletion analysis of four of eighteen late gene expression factor gene homologues of the baculovirus, BmNPV.

    abstract：:Nucleotide sequence analysis of the genome of the baculovirus Bombyx mori nuclear polyhedrosis virus (BmNPV) identified 18 homologues of the Autographa californica NPV (AcNPV) lefs (late expression factor genes). These BmNPV lefs showed high (73-98%) amino acid sequence identities to AcNPV lefs and were localized to s...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8457

    authors： Gomi S,Zhou CE,Yih W,Majima K,Maeda S

    更新日期：1997-03-31 00:00:00

  • Amphibian (Xenopus laevis) tadpoles and adult frogs mount distinct interferon responses to the Frog Virus 3 ranavirus.

    abstract：:Infections of amphibians by Frog Virus 3 (FV3) and other ranavirus genus members are significantly contributing to the amphibian declines, yet much remains unknown regarding amphibian antiviral immunity. Notably, amphibians represent an important step in the evolution of antiviral interferon (IFN) cytokines as they ar...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2017.01.001

    authors： Wendel ES,Yaparla A,Koubourli DV,Grayfer L

    更新日期：2017-03-01 00:00:00

  • The metabolism of host RNAs in cells infected by an adenovirus E4 mutant.

    abstract：:Mutants of adenovirus type 5 (Ad5) that lack early region 4 (E4) are defective in the expression of viral late genes. E4 mutants exhibit dramatically reduced levels of both cytoplasmic and nuclear viral late RNAs compared to wild-type virus, due principally to reduced stability of unprocessed viral late RNA in the nuc...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90498-z

    authors： Sandler AB,Ketner G

    更新日期：1991-03-01 00:00:00

  • The fusion protein of Peste des petits ruminants virus mediates biological fusion in the absence of hemagglutinin-neuraminidase protein.

    abstract：:To study the process of membrane fusion in Morbilliviruses, the fusion (F) glycoproteins of Peste des petits ruminants virus (PPRV) and Rinderpest virus (RPV) were expressed transiently in mammalian cells. The recombinant F proteins were found to be localized at the surface of transfected cells. The fusion activity, a...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.1120

    authors： Seth S,Shaila MS

    更新日期：2001-10-10 00:00:00

  • Conserved amino acids of the human immunodeficiency virus type 2 Vpx nuclear localization signal are critical for nuclear targeting of the viral preintegration complex in non-dividing cells.

    abstract：:The HIV-2 viral accessory protein Vpx is related to, but distinct from the Vpr protein of HIV-1. Vpx is packaged into virions and as a component of the viral preintegration complex (PIC) is required for efficient virus replication in non-dividing cells. We have previously reported that the minimal transferable region ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.10.036

    authors： Belshan M,Mahnke LA,Ratner L

    更新日期：2006-03-01 00:00:00

  • Domain- and nucleotide-specific Rev response element regulation of feline immunodeficiency virus production.

    abstract：:Computational analysis of feline immunodeficiency virus (FIV) RNA sequences indicated that common FIV strains contain a rev response element (RRE) defined by a long unbranched hairpin with 6 stem-loop sub-domains, termed stem-loop A (SLA). To examine the role of the RNA secondary structure of the RRE, mutational analy...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.04.009

    authors： Na H,Huisman W,Ellestad KK,Phillips TR,Power C

    更新日期：2010-09-01 00:00:00

  • Equine herpesvirus 1 (EHV-1) glycoprotein M: effect of deletions of transmembrane domains.

    abstract：:Equine herpesvirus 1 (EHV-1) recombinants that carry either a deletion of glycoprotein M (gM) or express mutant forms of gM were constructed. The recombinants were derived from strain Kentucky A (KyA), which also lacks genes encoding gE and gI. Plaques on RK13 cells induced by the gM-negative KyA were reduced in size ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0664

    authors： Seyboldt C,Granzow H,Osterrieder N

    更新日期：2000-12-20 00:00:00

  • Isolation of a monoclonal antibody that recognizes the origin binding domain of JCV, but not SV40, large T-antigen.

    abstract：:Within immunocompromised populations, the JC polyomavirus is the cause of the often-fatal disease Progressive Multifocal Leukoencephalopathy (PML). JC virus encodes a protein, termed T-antigen (T-ag), which is essential for its replication and pathogenicity. Previous studies of JCV T-ag have, in general, used antibodi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2016.07.006

    authors： Grubman SA,Shin J,Phelan PJ,Gong A,Can H,Dilworth R,Kini SK,Gagnon D,Archambault J,Meinke G,Bohm A,Jefferson DM,Bullock PA

    更新日期：2016-10-01 00:00:00

  • Determination of a splice acceptor site of pX gene in HTLV-I infected cells.

    abstract：:The splice acceptor site of pX gene of HTLV-I has been determined to be at base position 7301 using S1 nuclease protection analysis. This splice acceptor site is used in all HTLV-I immortalized T-cell clones studied despite variation in the abundance levels of pX mRNA. Our results confirmed the proposal by Haseltine e...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(85)90404-0

    authors： Okamoto T,Josephs SF,Kawanishi M,Wong-Staal F

    更新日期：1985-06-01 00:00:00

  • Role of the Drosophila genome in Sigma virus multiplication. I. Role of the ret(2)P gene; selection of host-adapted mutants at the nonpermissive allele Pp.

    abstract：:The allele Pp of gene ref(2)P of Drosophila melanogaster is known to inhibit the multiplication of certain strains of Sigma virus. From a viral strain for which the allele PP of ref(2)P is nonpermissive, host adapted (haP) mutants were selected after mutagenesis in vivo. Mutagenized virus was extracted from infected f...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(81)90227-0

    authors： Contamine D

    更新日期：1981-10-31 00:00:00

  • A human T-cell line resistant to cytopathic effects of the human immunodeficiency virus (HIV).

    abstract：:Infection of human helper T lymphocytes with the human immunodeficiency virus (HIV) results in a rapid induction of cytopathic effects and cell lysis. We isolated a variant of the human T-lymphoblastoid cell line, CEM, that is fully susceptible to HIV infection but resistant to virally induced cytopathic effects. Expo...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90434-x

    authors： Casareale D,Stevenson M,Sakai K,Volsky DJ

    更新日期：1987-01-01 00:00:00

  • Assessment of antibody responses against gp41 in HIV-1-infected patients using soluble gp41 fusion proteins and peptides derived from M group consensus envelope.

    abstract：:Human immunodeficiency virus type 1 (HIV-1) transmembrane glycoprotein gp41 is targeted by broadly-reactive neutralizing antibodies 2F5 and 4E10, making it an attractive target for vaccine development. To better assess immunogenic properties of gp41, we generated five soluble glutathione S-transferase fusion proteins ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.11.009

    authors： Penn-Nicholson A,Han DP,Kim SJ,Park H,Ansari R,Montefiori DC,Cho MW

    更新日期：2008-03-15 00:00:00