当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > Identification and characterization of an equine herpesvirus 1 late gene encoding a potential zinc finger.

Identification and characterization of an equine herpesvirus 1 late gene encoding a potential zinc finger.

Abstract：

:In this report, we present the DNA sequence and transcriptional characterization of a gene (IR5) that maps within each of the inverted repeat (IR) segments of the equine herpesvirus type 1 (EHV-1) genome. The IR5 open reading frame (ORF) is located within both IR sequences (nucleotides 9932-10,642 of the IR). DNA sequence analyses of the IR5 gene region revealed an ORF of 236 amino acids (24,793 Da) that showed significant homology to ORF64 of varicella-zoster virus and ORF3 of EHV-4 both of which map within the inverted repeats and to the US10 ORF of herpes simplex virus type 1 (HSV-1) which maps within the unique short segment. Additional analyses of the nucleotide sequence failed to reveal any overlapping ORFs that would correspond to US11 or US12 of HSV-1. Interestingly, the IR5 ORF of EHV-1 possesses a sequence of 13 amino acids (CAYWCCLGHAFAC) that is a perfect match to the consensus zinc finger motif (C-X2-4-C-X2-15-C/H-X2-4-C/H). Putative cis-acting elements flanking the IR5 ORF include a TATA box (nucleotides 9864-9870), a CAAT box (nucleotides 9709-9714), and a polyadenylation signal (nucleotides 10,645-10,650). Northern blot and S1 nuclease analyses identified a single 0.9-kb mRNA species that first appears at 2 hr postinfection, and whose synthesis is reduced in the presence of phosphonoacetic acid, an inhibitor of EHV-1 DNA synthesis. Thus, the IR5 gene of EHV-1 exhibits characteristics representative of a late gene of the gamma-1 class. The characterization of the IR5 gene at the DNA and RNA levels will facilitate ongoing studies to identify and characterize the IR5 polypeptide.

journal_name

Virology

journal_title

Virology

authors

Holden VR,Yalamanchili RR,Harty RN,O'Callaghan DJ

doi

10.1016/0042-6822(92)90525-t

subject

Has Abstract

pub_date

1992-06-01 00:00:00

pages

704-13

issue

2

eissn

0042-6822

issn

1096-0341

journal_volume

188

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • The roles of the red clover necrotic mosaic virus capsid and cell-to-cell movement proteins in systemic infection.

    abstract：:The red clover necrotic mosaic dianthovirus (RCNMV) genome is split between two single-stranded RNA species termed RNA-1 and RNA-2. RNA-2 is required for infection of whole plants but is dispensable for infection and virion formation in protoplasts. We have used full-length cDNA clones of RNA-1 and -2 from which infec...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1004

    authors： Xiong Z,Kim KH,Giesman-Cookmeyer D,Lommel SA

    更新日期：1993-01-01 00:00:00

  • Tick-borne flavivirus infection in Ixodes scapularis larvae: development of a novel method for synchronous viral infection of ticks.

    abstract：:Following a bite from an infected tick, tick-borne flaviviruses cause encephalitis, meningitis and hemorrhagic fever in humans. Although these viruses spend most of their time in the tick, little is known regarding the virus-vector interactions. We developed a simple method for synchronously infecting Ixodes scapulari...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.03.057

    authors： Mitzel DN,Wolfinbarger JB,Long RD,Masnick M,Best SM,Bloom ME

    更新日期：2007-09-01 00:00:00

  • Human papillomavirus type 16 E7 protein expressed in Escherichia coli and monkey COS-1 cells: immunofluorescence detection of the nuclear E7 protein.

    abstract：:Human papillomavirus type 16 E7 protein, expressed in Escherichia coli as fusion protein lac alpha peptide-E7 (lac-E7), was purified by electrophoresis and used to immunize rabbits to raise antisera. The anti-lac-E7 sera recognized another bacterially expressed fusion protein trpE-E7 by the Western blot method. The an...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90386-3

    authors： Sato H,Watanabe S,Furuno A,Yoshiike K

    更新日期：1989-05-01 00:00:00

  • The satellite RNA of barley yellow dwarf virus-RPV is supported by beet western yellows virus in dicotyledonous protoplasts and plants.

    abstract：:The subgroup II luteovirus barley yellow dwarf virus-RPV (BYDV-RPV) acts as a helper virus for a satellite RNA (satRPV RNA). The subgroup II luteovirus beet western yellows virus (BWYV) and the ST9-associated RNA (ST9a RNA), a BWYV-associated RNA that encodes a polymerase similar to those of subgroup I luteoviruses, w...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8532

    authors： Rasochová L,Passmore BK,Falk BW,Miller WA

    更新日期：1997-05-12 00:00:00

  • Identification and subcellular localization of a putative cell-to-cell transport protein from red clover mottle virus.

    abstract：:To investigate the mode of gene expression of red clover mottle virus (RCMV) middle component (M) RNA, we have synthesized an oligopeptide corresponding to the predicted carboxy-terminus of the RCMV counterparts of the cowpea mosaic virus (CPMV) 48K and 58K proteins. Using an antiserum raised against this synthetic ol...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90552-7

    authors： Shanks M,Tomenius K,Clapham D,Huskisson NS,Barker PJ,Wilson IG,Maule AJ,Lomonossoff GP

    更新日期：1989-12-01 00:00:00

  • Interleukin-6 and type 1 interferons inhibit varicella zoster virus replication in human neurons.

    abstract：:Varicella zoster virus (VZV) is a neurotropic alphaherpesvirus that, following primary infection (varicella), establishes latency in sensory, autonomic, sympathetic and parasympathetic neurons, where it remains until reactivation (zoster). VZV-specific cell-mediated immune responses maintain VZV latency; thus, immunos...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2018.06.013

    authors： Como CN,Pearce CM,Cohrs RJ,Baird NL

    更新日期：2018-09-01 00:00:00

  • Sequences homologous to two separate transforming regions of herpes simplex virus DNA are linked in two human genital tumors.

    abstract：:Ten human genital invasive squamous cell carcinomas and five human premalignant tissues were analyzed for the presence of selected sets of herpes simplex virus 2 (HSV-2) DNA sequences. Two vulvar tumors and one vulvar dysplastic tissue were found to contain DNA sequences homologous to the BglII O fragment (coordinates...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(86)90179-0

    authors： Manservigi R,Cassai E,Deiss LP,Di Luca D,Segala V,Frenkel N

    更新日期：1986-11-01 00:00:00

  • Helicase ATPase activity of the Tobacco mosaic virus 126-kDa protein modulates replicase complex assembly.

    abstract：:Mutations disrupting helicase domain motifs of the Tobacco mosaic virus 126/183-kDa proteins were investigated for their effect on replicase function and assembly. These mutations inhibited virus replication but did not affect 126-kDa induced N gene resistance or RNAi suppression. However, in vivo expressed 126-kDa mo...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.03.019

    authors： Wang X,Kelman Z,Culver JN

    更新日期：2010-07-05 00:00:00

  • Virulence and pathogenesis of the MSW and MSD strains of Californian myxoma virus in European rabbits with genetic resistance to myxomatosis compared to rabbits with no genetic resistance.

    abstract：:The pathogenesis of two Californian strains of myxoma virus (MSW and MSD) was examined in European rabbits (Oryctolagus cuniculus) that were either susceptible to myxomatosis (laboratory rabbits) or had undergone natural selection for genetic resistance to myxomatosis (Australian wild rabbits). MSW was highly lethal f...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.12.007

    authors： Silvers L,Inglis B,Labudovic A,Janssens PA,van Leeuwen BH,Kerr PJ

    更新日期：2006-04-25 00:00:00

  • Do hemagglutinin genes of highly pathogenic avian influenza viruses constitute unique phylogenetic lineages?

    abstract：:Avian influenza A viruses of the H5 and H7 subtypes periodically cause severe outbreaks of disease in poultry. The question we wished to address in this study is whether these highly pathogenic strains constitute unique lineages or whether they and related nonpathogenic viruses are derived from common ancestors in the...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1301

    authors： Röhm C,Horimoto T,Kawaoka Y,Süss J,Webster RG

    更新日期：1995-06-01 00:00:00

  • Physiological and molecular triggers for SARS-CoV membrane fusion and entry into host cells.

    abstract：:During viral entry, enveloped viruses require the fusion of their lipid envelope with host cell membranes. For coronaviruses, this critical step is governed by the virally-encoded spike (S) protein, a class I viral fusion protein that has several unique features. Coronavirus entry is unusual in that it is often biphas...

    journal_title：Virology

    pub_type： 杂志文章,评审

    doi：10.1016/j.virol.2017.12.015

    authors： Millet JK,Whittaker GR

    更新日期：2018-04-01 00:00:00

  • Adenovirus E1A proteins are closely associated with chromatin in productively infected and transformed cells.

    abstract：:The adenovirus E1A 243R oncoprotein encodes a potent transcription-repression function within the N-terminal 80 amino acids. Our proposed model of E1A repression predicts that E1A interacts with important cellular proteins on chromatin. Consistent with this idea, we report here that E1A proteins from in vivo formaldeh...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2007.10.021

    authors： Green M,Panesar NK,Loewenstein PM

    更新日期：2008-02-05 00:00:00

  • Role of a heterologous retroviral transport element in the development of genetic complementation assay for mouse mammary tumor virus (MMTV) replication.

    abstract：:The mouse mammary tumor virus (MMTV) is a type B retrovirus that is unique from other retroviruses in having multiple "tissue specific" and "hormone inducible" promoters. This unique feature has lead to the increasing interest in studying the biology of MMTV replication with the ultimate goal of developing MMTV based ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.12.027

    authors： Rizvi TA,Ali J,Phillip PS,Ghazawi A,Jayanth P,Mustafa F

    更新日期：2009-03-15 00:00:00

  • Targeting the hepatitis B virus precore antigen with a novel IgNAR single variable domain intrabody.

    abstract：:The Hepatitis B virus precore protein is processed in the endoplasmic reticulum (ER) into secreted hepatitis B e antigen (HBeAg), which acts as an immune tolerogen to establish chronic infection. Downregulation of secreted HBeAg should improve clinical outcome, as patients who effectively respond to current treatments...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.12.034

    authors： Walsh R,Nuttall S,Revill P,Colledge D,Cabuang L,Soppe S,Dolezal O,Griffiths K,Bartholomeusz A,Locarnini S

    更新日期：2011-03-01 00:00:00

  • Analysis of rubella virus E1 glycosylation mutants expressed in COS cells.

    abstract：:cDNA clones encoding the envelope glycoprotein E1 of rubella virus (RV) were altered by site-directed mutagenesis at consensus sites for addition of N-linked glycans. The resulting plasmids were introduced into COS cells and the mutant E1 proteins were analyzed by indirect immunofluorescence, radioimmunoprecipitation,...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90915-x

    authors： Hobman TC,Qiu ZY,Chaye H,Gillam S

    更新日期：1991-04-01 00:00:00

  • TCR triggering transcriptionally downregulates CCR5 expression on rhesus macaque CD4(+) T-cells with no measurable effect on susceptibility to SIV infection.

    abstract：:Studies using transformed human cell lines suggest that most SIV strains use CCR5 as co-receptor. Our analysis of primary rhesus macaque CD4(+) T-cell clones revealed marked differences in susceptibility to SIV(mac)239 infection. We investigated whether different levels of CCR5 expression account for clonal difference...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2010.10.005

    authors： Minang JT,Trivett MT,Barsov EV,Del Prete GQ,Trubey CM,Thomas JA,Gorelick RJ,Piatak M Jr,Ott DE,Ohlen C

    更新日期：2011-01-05 00:00:00

  • Molecular evolution of West Nile virus in a northern temperate region: Connecticut, USA 1999-2008.

    abstract：:West Nile virus (WNV) has become firmly established in northeastern US, reemerging every summer since its introduction into North America in 1999. To determine whether WNV overwinters locally or is reseeded annually, we examined the patterns of viral lineage persistence and replacement in Connecticut over 10 consecuti...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2011.06.006

    authors： Armstrong PM,Vossbrinck CR,Andreadis TG,Anderson JF,Pesko KN,Newman RM,Lennon NJ,Birren BW,Ebel GD,Henn MR

    更新日期：2011-08-15 00:00:00

  • Impact of route of exposure and challenge dose on the pathogenesis of H7N9 low pathogenicity avian influenza virus in chickens.

    abstract：:H7N9 influenza A first caused human infections in early 2013 in China. Virus genetics, histories of patient exposures to poultry, and previous experimental studies suggest the source of the virus is a domestic avian species, such as chickens. In order to better understand the ecology of this H7N9 in chickens, we evalu...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2015.01.013

    authors： Spackman E,Pantin-Jackwood M,Swayne DE,Suarez DL,Kapczynski DR

    更新日期：2015-03-01 00:00:00

  • Isoprenylation of large hepatitis delta antigen is necessary but not sufficient for hepatitis delta virus assembly.

    abstract：:Hepatitis delta virus (HDV) encodes two proteins, the small hepatitis delta antigen (SHDAg) and large hepatitis delta antigen (LHDAg). Both proteins are identical except for the presence of additional 19 amino acids at the C terminus of LHDAg. While SHDAg is required for HDV RNA replication, LHDAg inhibits replication...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1109

    authors： Lee CZ,Chen PJ,Lai MM,Chen DS

    更新日期：1994-02-15 00:00:00

  • HuR, a protein implicated in oncogene and growth factor mRNA decay, binds to the 3' ends of hepatitis C virus RNA of both polarities.

    abstract：:To identify cellular factors that interact with hepatitis C virus RNA, cellular extracts were subjected to UV cross-linking to radiolabeled RNAs corresponding to the hepatitis C virus 5' and 3' untranslated regions of positive and negative polarities. Our results demonstrate that the U-rich region of the hepatitis C v...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0461

    authors： Spångberg K,Wiklund L,Schwartz S

    更新日期：2000-09-01 00:00:00

  • Mutational profiling of the variability of individual amino acid positions in the hepatitis B virus matrix domain.

    abstract：:The hepatitis B virus (HBV) is formed by budding. A stretch of 22 amino acids (aa) (matrix domain, MD, R103 - S124) in the large envelope protein L is crucial for virion formation and probably establishes contact to the nucleocapsid. Here, we assess the impact of sequence variations at numerous individual aa positions...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2014.04.030

    authors： Schittl B,Bruss V

    更新日期：2014-06-01 00:00:00

  • The tobamovirus capsid protein functions as a host-specific determinant of long-distance movement.

    abstract：:The tobamoviruses, tobacco mosaic virus (TMV) and Odontoglossum ringspot virus (ORSV), differ in the range of plant species that each can systemically infect. Both viruses systemically infect Nicotiana benthamiana similarly, but differ in the ability to systemically infect tobacco (N. tabacum). ORSV is confined to the...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1993.1107

    authors： Hilf ME,Dawson WO

    更新日期：1993-03-01 00:00:00

  • Differential proteolytic activities of precursor and mature forms of the 24K proteinase of grapevine fanleaf nepovirus.

    abstract：:The presence of a genome-linked protein (VPg) at the RNA 5'-end of the genome is a characteristic of different groups of animal and plant positive-sense single-stranded RNA viruses. These viruses express their structural and functional proteins from polyproteins that are sequentially processed by at least one viral pr...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1165

    authors： Margis R,Viry M,Pinck M,Bardonnet N,Pinck L

    更新日期：1994-04-01 00:00:00

  • Mutational analysis of the small intergenic region of maize streak virus.

    abstract：:Maize streak virus (MSV) is a leafhopper-transmitted geminivirus containing one molecule of circular single-stranded DNA of about 2.7 kb. We have tested the infectivity of MSV mutants. A deletion of 29 bases in the small intergenic region (SIR) did not affect the infectivity of MSV. Mutants containing insertions of ol...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)91001-w

    authors： Shen WH,Hohn B

    更新日期：1991-08-01 00:00:00

  • The stability of the human papillomavirus E6 oncoprotein is E6AP dependent.

    abstract：:Human papillomavirus (HPV) E6 oncoproteins target numerous cellular proteins for ubiquitin-mediated degradation. In the case of p53 this is mediated by the E6AP ubiquitin ligase. However, there are conflicting reports concerning how central E6AP is to the global function of the HPV-16 and HPV-18 E6 oncoproteins. To in...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.07.029

    authors： Tomaić V,Pim D,Banks L

    更新日期：2009-10-10 00:00:00

  • Electron microscopy of binding of Epstein-Barr virus (EBV) nuclear antigen (EBNA-1) to EBV DNA.

    abstract：:Specific binding sites for Epstein-Barr virus (EBV) nuclear antigen (EBNA-1), isolated and semipurified from EBV-transformed nonproductive Raji cells, were visualized on the molecule of EBV DNA by electron microscopy and mapped. Two measures had to be applied to counteract the limited purity of the EBNA-1 preparation:...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90025-0

    authors： Reischig J,Bartsch D,Polack A,Vonka V,Hirsch I

    更新日期：1987-10-01 00:00:00

  • Sequence conservation and divergence of hepatitis delta virus RNA.

    abstract：:The complete RNA sequence of the hepatitis delta virus (HDV) obtained from the Nauru Island in the Pacific was determined by cDNA cloning and amplification by polymerase chain reaction (PCR). The sequence showed 14-17% divergence from the two known HDV RNA sequences. There are three highly conserved domains: the regio...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90335-o

    authors： Chao YC,Chang MF,Gust I,Lai MM

    更新日期：1990-10-01 00:00:00

  • Human papillomavirus type 18 variant lineages in United States populations characterized by sequence analysis of LCR-E6, E2, and L1 regions.

    abstract：:While HPV 16 variant lineages have been well characterized, the knowledge about HPV 18 variants is limited. In this study, HPV 18 nucleotide variations in the E2 hinge region were characterized by sequence analysis in 47 control and 51 tumor specimens. Fifty of these specimens were randomly selected for sequencing of ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.04.022

    authors： Arias-Pulido H,Peyton CL,Torrez-Martínez N,Anderson DN,Wheeler CM

    更新日期：2005-07-20 00:00:00

  • Helix-destabilizing properties of the baculovirus single-stranded DNA-binding protein (LEF-3).

    abstract：:The helix-destabilizing properties of a single-stranded DNA-binding protein, LEF-3, of Bombyx mori nucleopolyhedrovirus (BmNPV) were studied. Partial duplexes of DNA containing single-stranded (ss) tails of different sizes and orientations were used as substrates for assay of the unwinding ability of LEF-3. Upon nonco...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2000.0270

    authors： Mikhailov VS

    更新日期：2000-04-25 00:00:00

  • Membrane fusion of mumps virus with ghost erythrocytes and CV-1 cells.

    abstract：:The octadecyl rhodamine (R18) fluorescent dequenching assay was used to examine membrane fusion between mumps virus and mammalian cells. Rapid fluorescent dequenching, indicative of membrane fusion, was observed when labeled mumps virus was mixed with either ghost erythrocytes or CV-1 cells. After 15 min a saturation ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)90196-v

    authors： Di Simone C,Baldeschwieler JD

    更新日期：1992-11-01 00:00:00