Determination of a splice acceptor site of pX gene in HTLV-I infected cells.

Abstract:

:The splice acceptor site of pX gene of HTLV-I has been determined to be at base position 7301 using S1 nuclease protection analysis. This splice acceptor site is used in all HTLV-I immortalized T-cell clones studied despite variation in the abundance levels of pX mRNA. Our results confirmed the proposal by Haseltine et al. (W. A. Haseltine, J. Sodroski, R. Patarca, D. Briggs, D. Perkins, and F. Wong-Staal, Science (Washington, D. C. 225, 421-424 (1984); K. Shimotono, W. Wachsman, Y. Takahashi, D. W. Golde, M. Miwa, T. Sigimura, and I. S. Y. Chen, Proc. Natl. Acad. Sci. USA 81, 6657-6661 (1984)) that a pX protein with a molecular weight of at least 38,000 could be synthesized. Generation of a 2.0-kb pX mRNA may involve a double-splicing event.

journal_name

Virology

journal_title

Virology

authors

Okamoto T,Josephs SF,Kawanishi M,Wong-Staal F

doi

10.1016/0042-6822(85)90404-0

subject

Has Abstract

pub_date

1985-06-01 00:00:00

pages

636-9

issue

2

eissn

0042-6822

issn

1096-0341

journal_volume

143

pub_type

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