Abstract:
:The splice acceptor site of pX gene of HTLV-I has been determined to be at base position 7301 using S1 nuclease protection analysis. This splice acceptor site is used in all HTLV-I immortalized T-cell clones studied despite variation in the abundance levels of pX mRNA. Our results confirmed the proposal by Haseltine et al. (W. A. Haseltine, J. Sodroski, R. Patarca, D. Briggs, D. Perkins, and F. Wong-Staal, Science (Washington, D. C. 225, 421-424 (1984); K. Shimotono, W. Wachsman, Y. Takahashi, D. W. Golde, M. Miwa, T. Sigimura, and I. S. Y. Chen, Proc. Natl. Acad. Sci. USA 81, 6657-6661 (1984)) that a pX protein with a molecular weight of at least 38,000 could be synthesized. Generation of a 2.0-kb pX mRNA may involve a double-splicing event.
journal_name
Virologyjournal_title
Virologyauthors
Okamoto T,Josephs SF,Kawanishi M,Wong-Staal Fdoi
10.1016/0042-6822(85)90404-0subject
Has Abstractpub_date
1985-06-01 00:00:00pages
636-9issue
2eissn
0042-6822issn
1096-0341journal_volume
143pub_type
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