Abstract:
:Simian cells permissive for influenza A virus infection were stably transformed with a full-length cloned influenza A nucleoprotein gene under the control of an inducible metallothionein promoter and linked to a dihydrofolate reductase gene to facilitate cell selection. Transformed cells synthesized a virus-specific nucleoprotein which was indistinguishable from the nucleoprotein synthesized in virus-infected cells with respect to molecular weight and intracellular localization. It was estimated that transformed cells produced only 1% of the amount of nucleoprotein synthesized in simian cells infected with influenza A virus. Nonetheless, when transformed cells were infected with influenza virus mutants which synthesized temperature-sensitive nucleoprotein, protein expressed by the cloned gene was able to complement the synthesis of plus-strand and minus-strand viral RNA for one mutant and only plus-strand synthesis for another mutant. This indicated that the influenza A nucleoprotein expressed in the transformed cells exhibited functional activity.
journal_name
Virologyjournal_title
Virologyauthors
Ryan KW,Mackow ER,Chanock RM,Lai CJdoi
10.1016/0042-6822(86)90437-xsubject
Has Abstractpub_date
1986-10-15 00:00:00pages
144-54issue
1eissn
0042-6822issn
1096-0341journal_volume
154pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The nonstructural protein expressed from ORF-206 of turnip yellow mosaic virus is proteolytically processed to produce N-terminal 150-kDa and C-terminal 70-kDa proteins. Through the use of linker insertion and deletion analyses coupled to in vitro translation, we have delimited the protease domain to residues 731-885 ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1017
更新日期:1994-01-01 00:00:00
abstract::Morphological and molecular changes produced by Autographa californica nuclear polyhedrosis virus (AcMNPV) infection in a permissive cell line, IPLB-SF-21AE (SF-21), of Spodoptera frugiperda and a nonpermissive cell line, FPMI-CF-203 (CF-203), of Choristoneura fumiferana are described. CF-203 cells inoculated with AcM...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0411
更新日期:1996-08-01 00:00:00
abstract::Three prophage sequences were identified in the Lactobacillus johnsoni strain NCC533. Prophage Lj965 predicted a gene map very similar to those of pac-site Streptococcus thermophilus phages over its DNA packaging and head and tail morphogenesis modules. Sequence similarity linked the putative DNA packaging and head mo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0498
更新日期:2000-09-30 00:00:00
abstract::Demyelination of the human peripheral nervous system (PNS) can be caused by diverse mechanisms including viral infection. Despite association of several viruses with the development of peripheral demyelination, animal models of the condition have been limited to disease that is either autoimmune or genetic in origin. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.09.009
更新日期:2007-03-01 00:00:00
abstract::Interleukin 2-inducible T cell kinase (ITK) influences T cell signaling by coordinating actin polymerization and polarization as well as recruitment of kinases and adapter proteins. ITK regulates multiple steps of HIV-1 replication, including virion assembly and release. Fluorescent microscopy was used to examine the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.11.015
更新日期:2013-02-05 00:00:00
abstract::During the productive phase of chronic hepadnaviral infections, virion DNA synthesis occurs in the cytoplasm of the infected hepatocyte, but viral RNA is synthesized in the nucleus, apparently from a covalently closed, circular (CCC) viral DNA. J. Tuttleman, C. Pourcel, and J. Summers (1986a, Cell 47, 451-460) have sh...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90206-7
更新日期:1990-03-01 00:00:00
abstract::Genomic and messenger RNAs of a Zaire strain of Ebola virus were cloned, and inserts specific for the nucleoprotein gene were isolated and sequenced. The nucleoprotein gene is located proximal to the 3' end of the genome and is preceeded by a putative leader sequence. The gene begins with the transcriptional start sit...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90354-1
更新日期:1989-05-01 00:00:00
abstract::The growth of vesicular stomatitis virus requires two distinct RNA synthetic events: transcription of messenger RNA molecules and replication of the viral genome RNA. We report the use of a panel of monoclonal antibodies directed against the viral phosphoprotein P in an attempt to assess the role of this protein in RN...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0031
更新日期:1996-02-01 00:00:00
abstract::Porcine reproductive and respiratory syndrome virus (PRRSV) displays notorious genetic, antigenic, and clinical variability. Little is known, however, about the nature and extent of viral variation present within naturally infected animals. By amplifying and cloning the open reading frame 5 gene from tonsils of natura...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.07.009
更新日期:2003-12-20 00:00:00
abstract::The DNA of a rat cytomegalovirus (RCMV) isolate from England is cleaved by HindIII into 25 fragments, 20 of which have been cloned into recombinant plasmids. Twenty-two of thirty KpnI fragments were similarly cloned which, together with the HindIII clones, resulted in cloning of 93% of the viral genome. Terminal fragm...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90155-9
更新日期:1988-09-01 00:00:00
abstract::Chikungunya virus (CHIKV) represents a pandemic threat with no approved vaccine available. Recently, we described a novel vaccination strategy based on iDNA® infectious clone designed to launch a live-attenuated CHIKV vaccine from plasmid DNA in vitro or in vivo. As a proof of concept, we prepared iDNA plasmid pCHIKV-...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.01.009
更新日期:2016-03-01 00:00:00
abstract::Two mutant strains of cucumber mosaic virus (CMV) were investigated with respect to virion stability and molecular determinants of aphid vector transmission. The mutant 2A1-MT-60x, derived from the mechanically passaged wild type 2A1-AT, is poorly transmissible by the aphid Aphis gossypii and not transmissible by the ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0569
更新日期:2000-10-25 00:00:00
abstract::The display of foreign proteins on the surface of baculovirus virions has provided a tool for the analysis of protein-protein interactions and for cell-specific targeting in gene transfer applications. To evaluate the baculovirus display system as a vaccine vehicle, we have generated a recombinant baculovirus (AcNPV-C...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.08.003
更新日期:2003-11-10 00:00:00
abstract::The genomes of human herpes virus type-1 and type-2 share a high degree of sequence identity; yet, they exhibit important differences in pathology in their natural human host as well as in animal host and cell cultures. Here, we report the comparative analysis of the time and relative abundance profiles of the transcr...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.12.036
更新日期:2006-04-25 00:00:00
abstract::To illuminate the molecular basis for host range restriction of parainfluenza virus replication, we have examined the types of virus macromolecules produced during abortive infection of nonpermissive MDBK cells with human parainfluenza virus type 1 (hPIV1). While these cells do not support production of hPIV1 virus, t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0287
更新日期:1996-06-01 00:00:00
abstract::Endogenous feline leukemia viruses (enFeLVs) occur in the germ lines of the domestic cat and related wild species (genus Felis). We sequenced the long terminal repeats and part of the env region of enFeLVs in domestic cats and five wild species. A total of 305 enFeLV sequences were generated across 17 individuals, dem...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.06.010
更新日期:2010-09-30 00:00:00
abstract::Human norovirus is a dominant cause of acute gastroenteritis around the world. Several norovirus disinfectants label citric acid as an active ingredient. In this study, we showed that norovirus virus-like particles (VLPs) treated with citrate buffer caused the particles to alter their morphology, including increased d...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.07.009
更新日期:2015-11-01 00:00:00
abstract::We have analyzed the limiting factors involved in the induction of human immunodeficiency virus type 1 (HIV-1) provirus expression by tumor necrosis factor-alpha (TNF-alpha), phorbol-12-myristate-13-acetate (PMA), and bryostatin-1 in T-cells (ACH-2) and monocytes (U1). We have demonstrated that, while there is a corre...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90295-z
更新日期:1992-03-01 00:00:00
abstract::Immunoelectron microscopy using an anti-VP2 monoclonal antibody complexed to colloidal gold has been used to study the mechanism of bluetongue virus (BTV) release from infected cells. Examination of the BTV-infected cell surface revealed that viruses are released both as enveloped particles, by budding through the pla...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90218-3
更新日期:1989-11-01 00:00:00
abstract::The MC80R gene of molluscum contagiosum virus (MCV) type 1 encodes a major histocompatibility complex (MHC) class I homolog that lacks several amino-acid residues critical for peptide binding by MHC molecules, contains an unusually long N-terminal hydrophobic domain possibly derived by triplication of a signal peptide...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9390
更新日期:1998-10-25 00:00:00
abstract::Efferocytosis, the phagocytic clearance of apoptotic cells, can provide host protection against certain types of viruses by mediating phagocytic clearance of infected cells undergoing apoptosis. It is known that HIV-1 induces apoptosis and HIV-1-infected cells are efferocytosed by macrophages, although its molecular m...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.12.025
更新日期:2018-02-01 00:00:00
abstract::Twelve avian reovirus strain 176 proteins, 10 structural (lambda 1, lambda 2, lambda 3, mu 1, mu 2/mu 2C, sigma 1, sigma 2, sigma 3, and sigma 4), and 2 nonstructural (mu NS and sigma NS) were identified and characterized by electrophoretic analysis of purified virions and infected cell lysates. Three of the identifie...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1147
更新日期:1993-03-01 00:00:00
abstract::Feline immunodeficiency virus (FIV) is a lentivirus associated with an immunodeficiency syndrome of the domestic cat. A short open reading frame (ORF2), of unknown function, is present in all FIV isolates. We have investigated the role of ORF2 in determining the cell tropism of two infectious molecular clones of FIV. ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0002
更新日期:1996-01-01 00:00:00
abstract::West Nile virus (WNV) genome cyclization is mediated by two pairs of long-distance RNA/RNA interactions: the 5'CS/3'CSI (conserved sequence) and the 5'UAR/3'UAR (upstream AUG region) base pairings. Antisense peptide-conjugated phosphorodiamidate morpholino oligomers (PPMOs), designed to interfere with the 5'CS/3'CSI o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.08.044
更新日期:2008-12-05 00:00:00
abstract::In a previous study, we observed that hepatitis C virus (HCV) core protein specifically inhibits translation initiated by an HCV internal ribosome entry site (IRES). To investigate the mechanism by which down-regulation of HCV translation occurs, a series of mutations were introduced into the IRES element, as well as ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.10.013
更新日期:2006-02-20 00:00:00
abstract::Severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) caused a severe outbreak in several regions of the world in 2003. The SARS-CoV genome is predicted to contain 14 functional open reading frames (ORFs). The first ORF (1a and 1b) encodes a large polyprotein that is cleaved into nonstructural proteins (nsp)...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2007.04.029
更新日期:2007-09-30 00:00:00
abstract::Previously different authors described various flavivirus mutants with high affinity to cell glycosaminoglycans and low neuroinvasiveness in mice that were obtained consequently passages in cell cultures or in ticks. In present study the analysis of TBEV isolates has shown existence of GAG-binding variants in natural ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.12.012
更新日期:2010-03-15 00:00:00
abstract::Previous studies indicated that hepatitis C virus core protein influences cellular apoptosis. However, the precise mechanisms of the effects are not fully understood. Therefore, in this study, we examined the mechanisms of the effects on cell apoptosis by core protein, using transiently transfected and magnetically co...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1371
更新日期:2002-04-25 00:00:00
abstract::Attenuated mutants of Venezuelan equine encephalitis virus (VEE) were isolated by selection for rapid penetration of cultured cells (R. E. Johnston and J. F. Smith, 1988, Virology 162, 437-443). Sequence analysis of these mutants identified candidate attenuating mutations at four loci in the VEE E2 glycoprotein gene: ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90114-q
更新日期:1991-07-01 00:00:00
abstract::Replication of the satellite RNA of tobacco ringspot virus (sTobRV RNA) has been postulated to require rolling circle transcription. The expected product of rolling circle transcription, multimeric sTobRV RNA, is known to undergo self-cleavage in vitro to release unit-length sTobRV RNA. A spontaneous, efficient, not e...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90005-v
更新日期:1991-01-01 00:00:00