The nucleoprotein gene of Ebola virus: cloning, sequencing, and in vitro expression.

abstract：:In this study, we investigated the autoimmune response in rabbit ovaries following infection with a recombinant myxoma virus expressing rabbit zona pellucida protein B (MV-ZPB). A specific IgG antibody response to ZPB was elicited in the serum of infected rabbits and the antibody strongly bound to the zona pellucida o...

journal_title：Virology

authors： Gu W,Holland M,Janssens P,Seamark R,Kerr P

更新日期：2004-01-20 00:00:00

abstract：:Genetic variants surrounding the interferon-λ3 (IFNL3) gene are strongly associated with clearance of hepatitis C virus (HCV). A variant (rs368234815 TT/ΔG) upstream of IFNL3 was recently implicated to control expression of a novel gene termed IFNL4. We conducted genetic analysis of rs368234815 in a chronic HCV patien...

journal_title：Virology

authors： Lu YF,Goldstein DB,Urban TJ,Bradrick SS

更新日期：2015-02-01 00:00:00

abstract：:In this study, we show that adenoviral infection induced accumulation of the sphingolipid ceramide in a dose- and time-dependent manner. This accumulation preceded cell lysis, occurred in the absence of biochemical evidence of apoptosis, and was derived from de novo synthesis of ceramide. An adenovirus mutant that lac...

journal_title：Virology

authors： Kanj SS,Dandashi N,El-Hed A,Harik H,Maalouf M,Kozhaya L,Mousallem T,Tollefson AE,Wold WS,Chalfant CE,Dbaibo GS

更新日期：2006-02-05 00:00:00

abstract：:Following challenge with COPV (canine oral papillomavirus), DNA plasmids encoding COPV L1, E1 or E2 protein were delivered into oral mucosal and cutaneous sites in beagles using particle-mediated immunotherapeutic delivery (PMID). Two weeks post-challenge, a priming dose of 8 microg DNA was delivered followed by a boo...

journal_title：Virology

authors： Moore RA,Walcott S,White KL,Anderson DM,Jain S,Lloyd A,Topley P,Thomsen L,Gough GW,Stanley MA

更新日期：2003-09-30 00:00:00

abstract：:We have used cells infected with the HIV-1 molecular clone HX10 to study the binding of monoclonal antibodies (mAbs) to different epitopes within the extracellular domain of the HIV-1 transmembrane glycoprotein gp41. Gp41 mAb binding to the infected cells at 4 degrees was variable but weaker than the binding of an ant...

journal_title：Virology

authors： Sattentau QJ,Zolla-Pazner S,Poignard P

更新日期：1995-01-10 00:00:00

abstract：:The red clover necrotic mosaic dianthovirus capsid protein is expressed in vivo from a subgenomic RNA (sgRNA) identical to the 3'-terminal 1.5 kb of RNA-1. The 5' leader sequence of the capsid protein sgRNA is 62 nucleotides, contains a 14-nucleotide putative promoter sequence homologous to the RNA-1 5' terminus, and ...

journal_title：Virology

authors： Zavriev SK,Hickey CM,Lommel SA

更新日期：1996-02-15 00:00:00

abstract：:The 3' end of hepatitis E virus (HEV) contains cis-acting regulatory element, which plays an important role in viral replication. To develop specific replication inhibitor at the molecular level, mono- and di-hammerhead ribozymes (Rz) were designed and synthesized against the conserved 3' end sequences of HEV, which c...

journal_title：Virology

authors： Sriram B,Thakral D,Panda SK

更新日期：2003-08-01 00:00:00

abstract：:In order to investigate the genome organization of the porcine epidemic diarrhea virus (PEDV) further, cDNA clones covering the region between the nucleocapsid and the spike (S) protein genes were independently constructed and sequenced for the two virulent isolates Br1/87 and CV777. Of the three major ORFs identified...

journal_title：Virology

authors： Duarte M,Tobler K,Bridgen A,Rasschaert D,Ackermann M,Laude H

更新日期：1994-02-01 00:00:00

abstract：:Section of the sciatic nerve during the period of herpes simplex virus (HSV) latent infection was performed to evaluate residual latency in mouse dorsal root ganglion. In control mice without sciatic neurectomy, latency was present in 90-100%, while in those which underwent a neurectomy procedure, latent infection was...

journal_title：Virology

authors： Tenser RB,Edris WA,Hay KA

更新日期：1988-11-01 00:00:00

abstract：:Little is known about the neutralization properties of HIV-1 in India to optimally design and test vaccines. For this reason, a functional Env clone was obtained from each of ten newly acquired, heterosexually transmitted HIV-1 infections in Pune, Maharashtra. These clones formed a phylogenetically distinct genetic li...

journal_title：Virology

authors： Kulkarni SS,Lapedes A,Tang H,Gnanakaran S,Daniels MG,Zhang M,Bhattacharya T,Li M,Polonis VR,McCutchan FE,Morris L,Ellenberger D,Butera ST,Bollinger RC,Korber BT,Paranjape RS,Montefiori DC

更新日期：2009-03-15 00:00:00

abstract：:Replication-deficient adenovirus (Ad) vectors provide an efficient technology for direct DNA delivery to cells both in vitro and in vivo. We have inserted the measles virus nucleoprotein (N) gene under the control of the strong constitutive CMV major IE promoter into an Ad type 5 E1- vector to produce the recombinant ...

journal_title：Virology

authors： Fooks AR,Schadeck E,Liebert UG,Dowsett AB,Rima BK,Steward M,Stephenson JR,Wilkinson GW

更新日期：1995-07-10 00:00:00

abstract：:We have investigated a novel strategy for coexpressing two genes from a retroviral vector. The 5' nontranslated leader region of at least some picornavirus RNAs contains a sequence that can act as an internal ribosome entry site allowing initiation of translation at a downstream AUG codon in a 5' cap-independent manne...

journal_title：Virology

authors： Koo HM,Brown AM,Kaufman RJ,Prorock CM,Ron Y,Dougherty JP

更新日期：1992-02-01 00:00:00

abstract：:RNase protection analysis of mRNAs for Epstein-Barr virus (EBV) genes activated early in the induction of the productive cycle in Akata cells has confirmed that BZLF1 and BRLF1 behave as immediate early genes. These are likely to be the only virus immediate early genes since analysis of three other transcripts previou...

journal_title：Virology

authors： Packham G,Brimmell M,Cook D,Sinclair AJ,Farrell PJ

更新日期：1993-02-01 00:00:00

abstract：:Viroids are small, nontranslatable pathogenic RNAs that replicate autonomously and traffic systemically in their host plants. We have used in situ hybridization to analyze the trafficking pattern of Potato spindle tuber viroid (PSTVd) in tomato and Nicotiana benthamiana. When PSTVd was inoculated onto the stem of a pl...

journal_title：Virology

authors： Zhu Y,Green L,Woo YM,Owens R,Ding B

更新日期：2001-01-05 00:00:00

abstract：:To identify cellular factors that interact with hepatitis C virus RNA, cellular extracts were subjected to UV cross-linking to radiolabeled RNAs corresponding to the hepatitis C virus 5' and 3' untranslated regions of positive and negative polarities. Our results demonstrate that the U-rich region of the hepatitis C v...

journal_title：Virology

authors： Spångberg K,Wiklund L,Schwartz S

更新日期：2000-09-01 00:00:00

abstract：:Monoclonal antibodies to avian sarcoma/leukosis virus-coded transforming, structural, and polymerase proteins have been developed. In this first communication, a monoclonal antibody to pp60src will be characterized. The antibody can bind to pp60src in radioimmunoassays, precipitates this antigen in protein kinase test...

journal_title：Virology

authors： Tanaka T,Kurth R

更新日期：1984-02-01 00:00:00

abstract：:The complete RNA sequence of Sabin 3 (LED3) used in vaccine in the United States has been determined. The LED3 Sabin 3 sequence contains the attenuating mutations at bases 472 and 2034 but differs from that published by Stanway et al. (Nucleic Acids Res., 11, 5629-5643, 1983) at two other base positions, 2493 and 6061...

journal_title：Virology

authors： Weeks-Levy C,Tatem JM,DiMichele SJ,Waterfield W,Georgiu AF,Mento SJ

更新日期：1991-12-01 00:00:00

abstract：:The Epstein-Barr virus (EBV) predominantly establishes a latent infection in B lymphocytes, and occasionally switches from the latent state to the lytic cycle. In this report, we identified and examined the role of a lytic gene, BRRF2. We first prepared an antibody against BRRF2 and identified the gene product as a vi...

journal_title：Virology

authors： Watanabe T,Tsuruoka M,Narita Y,Katsuya R,Goshima F,Kimura H,Murata T

更新日期：2015-10-01 00:00:00

abstract：:A non-optimal receptor-binding specificity of avian influenza viruses is believed to hamper their replication in humans; however, the magnitude of this restriction remains undefined. Here we generated recombinant viruses, R1 and R2, that differed solely by two amino acids in the receptor-binding site of their hemagglu...

journal_title：Virology

authors： Matrosovich M,Matrosovich T,Uhlendorff J,Garten W,Klenk HD

更新日期：2007-05-10 00:00:00

abstract：:The yeast retrotransposon, Ty1, produces a macromolecular structure known as a virus-like particle (VLP) as an essential part of its replication cycle. The Ty1 Gag-like structural protein TYA, p1-440, alone is capable of directing assembly of the VLP. In order to determine the TYA sequences required for assembly, we h...

journal_title：Virology

authors： Brookman JL,Stott AJ,Cheeseman PJ,Adamson CS,Holmes D,Cole J,Burns NR

更新日期：1995-09-10 00:00:00

abstract：:Human Adenovirus Type 4 (HAdV-4) is responsible for epidemic outbreaks of Acute Respiratory Disease (especially in military recruits), and is known to cause significant morbidity with several reported cases of mortality. However, we do not understand why this serotype causes such high morbidity, and have little insigh...

journal_title：Virology

authors： Hartman ZC,Appledorn DM,Serra D,Glass O,Mendelson TB,Clay TM,Amalfitano A

更新日期：2008-05-10 00:00:00

abstract：:A range of deletion and other mutants in the coding region of the E1A gene of Ad5 has been assayed for transformation of baby rat kidney (BRK) cells in cooperation with ras, repression of the SV40 enhancer, and induction of proliferating cell nuclear antigen (PCNA). Transformation efficiency was drastically reduced by...

journal_title：Virology

authors： Jelsma TN,Howe JA,Mymryk JS,Evelegh CM,Cunniff NF,Bayley ST

更新日期：1989-07-01 00:00:00

abstract：:The influenza PA-X protein is translated from the PA open reading frame from frameshifting and suppresses cellular gene expression due to its ribonuclease activity. We further defined the functional roles of PA-X by comparing PA-X proteins from two related viruses - equine influenza (EIV) and canine influenza (CIV) H3...

journal_title：Virology

authors： Feng KH,Sun M,Iketani S,Holmes EC,Parrish CR

更新日期：2016-09-01 00:00:00

abstract：:Three different protein molecules copurify with T4 tail tubes after the tubes are released from the baseplate by guanidine hydrochloride treatment. These tube-associated proteins (TAPs) are the products of genes 29, 48, and 54. To further investigate the structural roles that these proteins may play in T4 tail assembl...

journal_title：Virology

authors： Ishimoto LK,Ishimoto KS,Cascino A,Cipollaro M,Eiserling FA

更新日期：1988-05-01 00:00:00

abstract：:Bovine herpesvirus 1 (BHV-1) gene expression was examined by RNA blot hybridization using clones representing immediate-early, early, and late genes. An immediate-early protein gene probe hybridized with two transcripts, 3.4 and 5.8 kb, expressed by infected cells in the presence of cycloheximide (CH). During infectio...

journal_title：Virology

authors： Seal BS,Whetstone CA,Zamb TJ,Bello LJ,Lawrence WC

更新日期：1992-05-01 00:00:00

abstract：:Progressive reorganization and depolymerization of microtubules corresponded with virus-induced rounding of Autographa californica M nuclear polyhedrosis virus (AcMNPV)-infected Spodoptera frugiperda IPLB-Sf-21 cells, suggesting that microtubules were instrumental in maintaining the normal shape of these cells. Depoly...

journal_title：Virology

authors： Volkman LE,Zaal KJ

更新日期：1990-03-01 00:00:00

abstract：:Herpes simplex virus 1 glycoprotein B (gB) is one of 10 glycoproteins in the virion envelope and in the membranes of infected cells. It is required for infection of cells in culture and functions in penetration of the cell by fusing the virion envelope with the plasma membrane. In studies to map the functional domains...

journal_title：Virology

authors： Navarro D,Paz P,Pereira L

更新日期：1992-01-01 00:00:00

abstract：:Open reading frame 89 (ORF89) is one of the three genes that are believed to be involved in the latent infection of white spot syndrome virus (WSSV). Here, we report the structure and functional characterization of ORF89. cDNA sequencing, 5' RLM-RACE, and 3' RLM-RACE showed that ORF89 gene is transcribed into an unspl...

journal_title：Virology

authors： Hossain MS,Khadijah S,Kwang J

更新日期：2004-07-20 00:00:00

abstract：:Following infection with murine cytomegalovirus (MCMV), the termini of the linear double-stranded DNA genome fuse to form circular or concatemeric forms which serve as replicative intermediates. To investigate the mechanisms involved in the generation and cleavage of the intracellular concatenates, we have used restri...

journal_title：Virology

authors： Marks JR,Spector DH

更新日期：1988-01-01 00:00:00

abstract：:The mature Gn glycoprotein of Crimean Congo hemorrhagic fever (CCHF) virus contains two predicted glycosylation sites (557N and 755N). Of these, N-glycans are added only at 557N, as evidenced by abrogation of Gn-glycosylation by mutation of 557N but not 755N site. Mutational block of Gn-glycosylation at 557N did not s...

journal_title：Virology

authors： Erickson BR,Deyde V,Sanchez AJ,Vincent MJ,Nichol ST

更新日期：2007-05-10 00:00:00