Abstract:
:The complete RNA sequence of Sabin 3 (LED3) used in vaccine in the United States has been determined. The LED3 Sabin 3 sequence contains the attenuating mutations at bases 472 and 2034 but differs from that published by Stanway et al. (Nucleic Acids Res., 11, 5629-5643, 1983) at two other base positions, 2493 and 6061. The change at base 6061 is silent and does not affect amino acid composition. The other base, a C at position 2493, is contained in the viral capsid protein VP1 and predicts a new Sabin 3 specific amino acid change of a threonine instead of an isoleucine at amino acid 6 of the protein [corrected]. Reversion of this base to that present in the pathogenic progenitor strain, Leon, is observed to occur after replication of vaccine virus in the gut of primary vaccines and in nervous tissue of neurovirulence test monkeys. Passage conditions have been identified that lead to the reversion of base 2493 as well as the reversion of the attenuated base to the parental base (Leon) at position 472 in the 5' noncoding region. The observation that these two bases delta position are found to revert during passage suggests that there is a selective advantage for virus containing the parental bases at these positions.
journal_name
Virologyjournal_title
Virologyauthors
Weeks-Levy C,Tatem JM,DiMichele SJ,Waterfield W,Georgiu AF,Mento SJdoi
10.1016/0042-6822(91)90576-wsubject
Has Abstractpub_date
1991-12-01 00:00:00pages
934-7issue
2eissn
0042-6822issn
1096-0341pii
0042-6822(91)90576-Wjournal_volume
185pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::CD8 T cells play an important role in controlling Ebola infection and in mediating vaccine-induced protective immunity, yet little is known about antigenic targets in Ebola that are recognized by CD8 T cells. Overlapping peptides were used to identify major histocompatibility complex class I-restricted epitopes in mic...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2003.09.016
更新日期:2004-01-05 00:00:00
abstract::The Hepatitis B virus precore protein is processed in the endoplasmic reticulum (ER) into secreted hepatitis B e antigen (HBeAg), which acts as an immune tolerogen to establish chronic infection. Downregulation of secreted HBeAg should improve clinical outcome, as patients who effectively respond to current treatments...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.12.034
更新日期:2011-03-01 00:00:00
abstract::The expression of two small basic proteins (C and C') encoded by a second open reading frame of the New Jersey serotype of vesicular stomatitis virus (VSV) P gene was reported previously (Spiropoulou and Nichol, J. Virol., 67, 3103-3110, 1993). Here we found that the Indiana serotype virus also expressed C and C' prot...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0066
更新日期:1996-02-15 00:00:00
abstract::The existence and genetic make-up of most primate retroviruses was revealed by studies of bushmeat and fecal samples from unhabituated primate communities. For these, detailed data on intra- and within-species contact rates are generally missing, which makes identification of factors influencing transmission a challen...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.05.012
更新日期:2014-07-01 00:00:00
abstract::Mannose binding lectin (MBL) generally plays a protective role during viral infection, yet MBL-mediated complement activation promotes Ross River virus (RRV)-induced inflammatory tissue destruction, contributing to arthritis and myositis. As MBL binds to carbohydrates, we hypothesized that N-linked glycans on the RRV ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2017.12.022
更新日期:2018-02-01 00:00:00
abstract::To determine if sequence variations observed in cosmopolitan HTLV-I interfered with viral recognition by neutralizing antibodies, we evaluated the neutralization potential of sera from persons infected by HTLV-I of this clade selected for amino acid changes in their eny glycoproteins. Each serum was used to neutralize...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9139
更新日期:1998-05-25 00:00:00
abstract::It has recently been shown that RNA from the bottom component of cowpea mosaic virus is capable of self-replication in cowpea mesophyll protoplasts, providing a tool for the investigation of the contribution of each RNA strand toward viral multiplication. Evidence is presented here, using this system, to show that the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(80)90239-1
更新日期:1980-10-15 00:00:00
abstract::The hypervariable region 1 (HVR1) of the putative envelope 2 protein of the hepatitis C virus (HCV) is the most variable part of the whole HCV polyprotein. Anti-HVR1 antibodies have been shown to protect against HCV infection, indicating that this region contains an important neutralization determinant. Recently we an...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0238
更新日期:2000-04-10 00:00:00
abstract::The sequences of the 3'-noncoding regions (NCR) of 12 strains of tick-borne encephalitis (TBE) virus were analyzed and found to vary in length from 350 to approximately 750 nucleotides. The size heterogeneity is restricted to a variable region following the stop codon, whereas the most 3'-terminal 350 nucleotides form...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.1557
更新日期:1995-10-20 00:00:00
abstract::IL-17-producing (Th17) and regulatory T (Treg) cells have been well established in the pathogenesis of many inflammatory diseases. To assess whether Th17 and Treg were altered in acute virus-induced myocarditis (AVMC) mice, we assessed Th17/Treg functions on different levels in AVMC. It was shown that the expression o...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2011.09.006
更新日期:2011-12-05 00:00:00
abstract::To elucidate the mechanism of formation of cowpea mosaic virus (CPMV) particles, RNA-2-encoded precursor proteins were expressed in Spodoptera frugiperda cells. Processing of the 105K and 95K polyproteins in trans to give the mature Large (L) and Small (S) coat proteins required both the 32K proteinase cofactor and th...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.08.023
更新日期:2009-10-25 00:00:00
abstract::Toscana (TOS) virus stocks strongly interfering with standard virus replication were obtained by sequential passages of virus in suckling mouse brain. Characterization of viral RNAs in these stocks showed the presence of a heterogeneous population of defective RNA molecules derived from the L genomic segment, in both ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9195
更新日期:1998-06-20 00:00:00
abstract::Binding of a specific peptide(s) from a viral protein to major histocompatibility complex (MHC) class I molecules is a critical step in the activation of CD8(+) cytotoxic T lymphocytes (CTLs). Once activated, CTLs can cause lethal disease in an infected host, for example, by killing virus-containing ependymal and vent...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9593
更新日期:1999-04-10 00:00:00
abstract::Some retroviruses, including HIV-1, regulate the relative amounts of gag and pol gene products by a translational frameshift mechanism. The consequences of altering the ratios of the Gag and Pol proteins were tested using vaccinia virus expression vectors, in which the gag and pol genes were fused by placing them in t...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1174
更新日期:1993-04-01 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) Tat transcriptionally activates expression from a number of viral and cellular promoters. Recent studies demonstrate the ability of Tat to differentially modulate cellular responses to apoptotic signaling. The antiapoptotic effects of Tat appear to correlate with increased e...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9688
更新日期:1999-05-10 00:00:00
abstract::The human polyomavirus BK virus (BKV) is an important opportunistic pathogen whose disease prevalence continues to increase with the growing immunocompromised population. To date, the major determinant of replication in cell culture has not been formally proven. BKV exists as archetype virus and rearranged variants, w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.08.032
更新日期:2010-11-25 00:00:00
abstract::The v-erb B protein of avian erythroblastosis virus (AEV) possesses an associated protein kinase activity in vitro. Analysis of temperature-sensitive mutants, and nonconditional host range mutants of AEV demonstrated that there was no simple correlation between this autophosphorylation activity and the transformation ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(86)90287-4
更新日期:1986-04-15 00:00:00
abstract::Synthesis of hepadnaviral DNA is dependent upon both the viral DNA polymerase and the viral core protein, the subunit of the nucleocapsids in which viral DNA synthesis takes place. In a study of natural isolates of duck hepatitis B virus (DHBV), we cloned full-length viral genomes from a puna teal. One of the clones f...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.06.004
更新日期:2006-09-30 00:00:00
abstract::The response to influenza virus (IAV) infection and severity of disease is highly variable in humans. We hypothesized that one factor contributing to this variability is the presence of specific respiratory tract (RT) microbes. One such microbe is Streptococcus pneumoniae (Sp) that is carried asymptomatically in the R...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.06.019
更新日期:2014-08-01 00:00:00
abstract::We have characterized the fibergenes of adenovirus type 19p (Ad19p), Ad19a, and Ad37 by sequencing. The fiber genes of Ad19a and Ad37 are identical and only five amino acids differ comparing Ad19a/Ad37 with Ad19p. Based on the translated sequences we calculated the isoelectrical points (Ips) and found that the fiber k...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.8269
更新日期:1997-01-06 00:00:00
abstract::H5 and H7 avian influenza viruses can become highly pathogenic in chickens after interspecies transmission. These viruses have transmitted directly to humans from birds in Eurasia and Africa (H5N1), the Netherlands (H7N7), and Canada (H7N3). Here we report antigenic, sequence, and phylogenetic analyses of H7N3 viruses...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.05.008
更新日期:2009-08-01 00:00:00
abstract::We have previously shown that peroxisome proliferator-activated receptor-gamma (PPARgamma) agonists inhibited the inflammatory response of RSV-infected human lung epithelial cells. In this study, we supply evidence that specific PPARgamma agonists (15d-PGJ2, ciglitazone, troglitazone, Fmoc-Leu) efficiently blocked the...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.03.008
更新日期:2006-07-05 00:00:00
abstract::Spontaneous proteolysis of influenza virus M1 protein during crystallisation has defined an N-terminal domain of amino acids 1--164. Full-length M1, the N-terminal domain, and the C-terminal part of M1 (residues 165--252) were produced in Escherichia coli. In vitro tests showed that only full-length M1 and its N-termi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0804
更新日期:2001-03-01 00:00:00
abstract::Genetically engineered mice with targeted mutations in genes encoding immunologically relevant molecules were used to elucidate the role of different immune effector mechanisms in protection against a rabies virus (RV) infection. In vaccinated animals challenged with a highly virulent strain of RV, antibodies were cru...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1995.0049
更新日期:1995-12-20 00:00:00
abstract::The natural host of SIVrcm is the red-capped mangabey (Cercocebus torquatus torquatus). Although this virus infects macaques and human PBMCs, its pathogenic potential is unknown. We serially passaged SIVrcm through 9 rhesus macaques to assess its potential for virulence. SIVrcm infected all macaques with peak viremia ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.012
更新日期:2008-09-15 00:00:00
abstract::The lung-derived dendritic cell (LDC) recruitment following intranasal (i.n.) vaccination of different poxviral vector-based vaccines/adjuvants were evaluated to decipher how these factors influenced CD8(+) T cell avidity. Compared to the standard i.n. recombinant fowlpox virus (FPV)-HIV vaccination, the FPV-HIV IL-13...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.09.004
更新日期:2014-11-01 00:00:00
abstract::The sequence of Broadhaven (BRD) virus segment 5, the major genetic determinant of serotype, is 1658 nucleotides in length and contains a single open reading frame (ORF) having the coding capacity for a protein of Mr 52.5K. Comparison of the ORF of segment 5 of BRD virus with published sequences of bluetongue virus (B...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90320-q
更新日期:1990-11-01 00:00:00
abstract::Expression of foreign peptides on the surface of cowpea mosaic virus particles leads to the creation of chimaeras with a variety of phenotypes and yields. Two factors were shown to be particularly significant in determining the properties of a given chimaera: the length of the inserted sequence and its isoelectric poi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00140-5
更新日期:2003-05-25 00:00:00
abstract::The N-terminal domains of the NS1 protein of influenza B virus (NS1B protein) and the NS1 protein of influenza A virus (NS1A protein) share one function: binding double-stranded RNA (dsRNA). Here we show that the N-terminal domain of the NS1B protein possesses an additional function that is not shared by its NS1A coun...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2002.1663
更新日期:2002-12-20 00:00:00
abstract::Infection of human helper T lymphocytes with the human immunodeficiency virus (HIV) results in a rapid induction of cytopathic effects and cell lysis. We isolated a variant of the human T-lymphoblastoid cell line, CEM, that is fully susceptible to HIV infection but resistant to virally induced cytopathic effects. Expo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90434-x
更新日期:1987-01-01 00:00:00