Abstract:
:Viroids are small, nontranslatable pathogenic RNAs that replicate autonomously and traffic systemically in their host plants. We have used in situ hybridization to analyze the trafficking pattern of Potato spindle tuber viroid (PSTVd) in tomato and Nicotiana benthamiana. When PSTVd was inoculated onto the stem of a plant, it replicated and trafficked to sink, but not source, leaves. PSTVd was absent from shoot apical meristems. In the flowers of infected plants, PSTVd was present in the sepals, but was absent in the petals, stamens, and ovary. The replicative form of PSTVd was detected in the phloem. Our data demonstrate that (i) PSTVd traffics long distance in the phloem and this trafficking is likely sustained by replication of the viroid in the phloem, and (ii) PSTVd trafficking is governed by plant developmental and cellular factors. The dependency of PSTVd and other viroids on cellular mechanisms for RNA trafficking makes them excellent tools to study such mechanisms.
journal_name
Virologyjournal_title
Virologyauthors
Zhu Y,Green L,Woo YM,Owens R,Ding Bdoi
10.1006/viro.2000.0724subject
Has Abstractpub_date
2001-01-05 00:00:00pages
69-77issue
1eissn
0042-6822issn
1096-0341pii
S0042-6822(00)90724-4journal_volume
279pub_type
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