Abstract:
:Although it is known that Ad5-specific neutralizing antibodies (NAbs) against three major viral capsid components (hexon, penton and fiber) are generated, differences in the frequency and nature of these pre-existing NAbs remain unclear. The results emphasized the contribution of anti-fiber antibodies to Ad5 neutralization responses generated during natural viral infection. Additionally, Ad5-specific NAbs against the fiber knob protein were present in over 90% of the positive serum samples while 42% of the sera had NAbs against hexon in this study based on neutralization assay of anti-HVR and anti-knob subtracted sera and Western blotting analysis. We also found that the trimeric knob was preferentially recognized by fiber-induced NAbs and it was serotype-specific in human adenovirus species C. Results indicated that the trimeric knob protein would be a good candidate antigen for detecting adenovirus serotype-specific NAbs in naturally infected sera.
journal_name
Virologyjournal_title
Virologyauthors
Yu B,Dong J,Wang C,Zhan Y,Zhang H,Wu J,Kong W,Yu Xdoi
10.1016/j.virol.2012.12.014subject
Has Abstractpub_date
2013-03-15 00:00:00pages
118-23issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(12)00642-3journal_volume
437pub_type
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