Analysis of TYA protein regions necessary for formation of the Ty1 virus-like particle structure.

Abstract:

:The yeast retrotransposon, Ty1, produces a macromolecular structure known as a virus-like particle (VLP) as an essential part of its replication cycle. The Ty1 Gag-like structural protein TYA, p1-440, alone is capable of directing assembly of the VLP. In order to determine the TYA sequences required for assembly, we have produced a series of truncated and deleted TYA forms and assessed their ability to assemble into particles. Removal of 100 amino acids from the C-terminus renders the TYA protein, p1-340, incapable of particle assembly; however, p1-363 with 77 residues missing from the C-terminus is capable of assembly. Removal of 40 amino acids from the N-terminus (p41-440 and p41-381) does not affect particle formation but more severely N-truncated forms, p71-381 and p100-381, are present as large aggregates within the cells and are therefore either incapable of or unavailable for VLP formation. Analysis of an internally deleted TYA, p1-381 delta 62-114, has identified this as a possible region of the TYA protein important for subunit:subunit interactions during the particle assembly.

journal_name

Virology

journal_title

Virology

authors

Brookman JL,Stott AJ,Cheeseman PJ,Adamson CS,Holmes D,Cole J,Burns NR

doi

10.1006/viro.1995.1454

subject

Has Abstract

pub_date

1995-09-10 00:00:00

pages

69-76

issue

1

eissn

0042-6822

issn

1096-0341

pii

S0042-6822(85)71454-7

journal_volume

212

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Infection, replication, and cytopathology of human papillomavirus type 31 in trophoblasts.

    abstract::Human papillomavirus (HPV) DNA is preferentially found in spontaneous abortions, specifically residing in trophoblasts, and transfected HPV-16 DNA replicates and produces progeny in 3A trophoblasts in culture. In this study 3A trophoblasts were shown to display both HPV receptors and infection by HPV-31b and HPV-6 vir...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2003.08.020

    authors: You H,Liu Y,Agrawal N,Prasad CK,Chiriva-Internati M,Lowery CL,Kay HH,Hermonat PL

    更新日期:2003-11-25 00:00:00

  • Antigenicity and vaccine potential of Marburg virus glycoprotein expressed by baculovirus recombinants.

    abstract::There is no effective vaccine for Marburg virus (MBGV) or any other filovirus, nor enough pertinent information to expedite rational vaccine development. To ascertain some of the minimal requirements for a MBGV vaccine, we determined whether whole inactivated MBGV, or a baculovirus-expressed virion subunit, could be u...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1997.8883

    authors: Hevey M,Negley D,Geisbert J,Jahrling P,Schmaljohn A

    更新日期:1997-12-08 00:00:00

  • Potato virus X coat protein: a glycoprotein.

    abstract::The coat protein (CP) genes of all the strains of potato virus X (PVX) code for a protein of 25 kDa. Analysis of the CP by SDS-PAGE shows a migration mobility of 27 to 29 kDa, depending of the strain. Amino acid identity between some strains is too high to explain such abnormal migration by differences in primary stru...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1994.1386

    authors: Tozzini AC,Ek B,Palva ET,Hopp HE

    更新日期:1994-08-01 00:00:00

  • Murine leukemia virus maturation: protease region required for conversion from "immature" to "mature" core form and for virus infectivity.

    abstract::Murine leukemia virus (MuLV) genome encodes a protease (Y. Yoshinaka, I. Katoh, T.D. Copeland, and S. Oroszlan (1985), Proc. Natl. Acad. Sci. USA 82, 1618-1622), which has been shown to cause maturation, specified as morphological conversion from "immature" to "mature" form of virus cores. To examine whether "immature...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(85)90161-8

    authors: Katoh I,Yoshinaka Y,Rein A,Shibuya M,Odaka T,Oroszlan S

    更新日期:1985-09-01 00:00:00

  • Physiological and molecular triggers for SARS-CoV membrane fusion and entry into host cells.

    abstract::During viral entry, enveloped viruses require the fusion of their lipid envelope with host cell membranes. For coronaviruses, this critical step is governed by the virally-encoded spike (S) protein, a class I viral fusion protein that has several unique features. Coronavirus entry is unusual in that it is often biphas...

    journal_title:Virology

    pub_type: 杂志文章,评审

    doi:10.1016/j.virol.2017.12.015

    authors: Millet JK,Whittaker GR

    更新日期:2018-04-01 00:00:00

  • Effect of 5' and 3' terminal sequences, overall length, and coding capacity on the accumulation of defective RNAs associated with broad bean mottle bromovirus in planta.

    abstract::Broad bean mottle bromovirus (BBMV) was shown to accumulate RNA2-derived defective interfering (DI) RNAs [Romero et al., Virology 194, 576-584 (1993); Pogany et al., Virology 212, 574-586 (1995)]. In this work, we utilize three sets of BBMV RNA2-derived artificial DI RNA constructs to determine factors that affect the...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1996.8377

    authors: Pogany J,Romero J,Bujarski JJ

    更新日期:1997-02-17 00:00:00

  • Lipid selection in the assembly of the phospholipid bilayer membrane of the lipid-containing bacteriophage PR4.

    abstract::Phage PR4 contains a lipid bilayer within the phage protein capsid. The phospholipids of the bilayer are derived from those of the host. We report that phage morphogenesis selects against the unusually bulky phospholipids synthesized by Escherichia coli grown in the presence of various sugar alcohols. These data indic...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1994.1004

    authors: Myung H,Cronan JE Jr

    更新日期:1994-01-01 00:00:00

  • Rescue of a 7502-nucleotide (49.3% of full-length) synthetic analog of respiratory syncytial virus genomic RNA.

    abstract::A cDNA was constructed to encode an internally-truncated version of negative-sense genomic RNA (vRNA) of respiratory syncytial virus (RSV) containing (in 3' to 5' order) the 3' vRNA leader region, the complement of the open reading frame for bacterial chloramphenicol acetyl transferase (CAT) under the control of RSV g...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1993.1368

    authors: Collins PL,Mink MA,Hill MG 3rd,Camargo E,Grosfeld H,Stec DS

    更新日期:1993-07-01 00:00:00

  • Studies of influenza haemagglutinin-mediated membrane fusion.

    abstract::A resonance energy transfer assay of membrane fusion developed by P. S. Uster and D. W. Deamer (Biochemistry 24, 1-8 (1985)) was used in a study of influenza haemagglutinin-mediated fusion. The characteristics of fusion and haemolysis by X-31 (H3N2) virus, a number of mutants of X-31 which fuse membranes at higher pH,...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(86)90083-8

    authors: Wharton SA,Skehel JJ,Wiley DC

    更新日期:1986-02-01 00:00:00

  • African swine fever virus EP153R open reading frame encodes a glycoprotein involved in the hemadsorption of infected cells.

    abstract::The open reading frame EP153R, located within the EcoRI E' fragment of the African swine fever (ASF) virus genome, is predicted to encode a membrane protein of 153 amino acids that presents significant homology to the N-terminal region of several CD44 molecules. EP153R contains multiple putative sites for N-glycosylat...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1999.0080

    authors: Galindo I,Almazán F,Bustos MJ,Viñuela E,Carrascosa AL

    更新日期:2000-01-20 00:00:00

  • Identification of tolerated insertion sites in poliovirus non-structural proteins.

    abstract::Insertion of nucleotide sequences encoding "tags" that can be expressed in specific viral proteins during an infection is a useful strategy for purifying viral proteins and their functional complexes from infected cells and/or for visualizing the dynamics of their subcellular location over time. To identify regions in...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2010.09.028

    authors: Teterina NL,Lauber C,Jensen KS,Levenson EA,Gorbalenya AE,Ehrenfeld E

    更新日期:2011-01-05 00:00:00

  • Unique physicochemical properties of human enteric Ad41 responsible for its survival and replication in the gastrointestinal tract.

    abstract::Human enteric adenovirus Ad41 is associated with children gastroenteritis. To infect gastrointestinal cells, the invading virus must be acid-stable and resistant to inactivation by bile salts and proteases. In addition, it has to cross the mucus barrier before it infects mucosa cells. We show that Ad41 infectivity is ...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2004.01.020

    authors: Favier AL,Burmeister WP,Chroboczek J

    更新日期:2004-04-25 00:00:00

  • Specific restrictions in the progression of Venezuelan equine encephalitis virus-induced disease resulting from single amino acid changes in the glycoproteins.

    abstract::The pathogenesis of Venezuelan equine encephalitis virus (VEE) was examined in the mouse model using V3000, a virus derived from a molecular clone of the Trinidad donkey strain of VEE. These results were compared in parallel experiments with avirulent mutants of VEE derived by site-directed mutagenesis of the clone. A...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1995.1022

    authors: Grieder FB,Davis NL,Aronson JF,Charles PC,Sellon DC,Suzuki K,Johnston RE

    更新日期:1995-02-01 00:00:00

  • Carboxy-fluorescein diacetate, succinimidyl ester labeled papillomavirus virus-like particles fluoresce after internalization and interact with heparan sulfate for binding and entry.

    abstract::Human papillomaviruses (HPVs) infect epithelial cells and are associated with genital carcinoma. Most epithelial cell lines express cell-surface glycosaminoglycans (GAGs) usually found attached to the protein core of proteoglycans. Our aim was to study how GAGs influenced HPV entry. Using a human keratinocyte cell lin...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/s0042-6822(03)00114-4

    authors: Drobni P,Mistry N,McMillan N,Evander M

    更新日期:2003-05-25 00:00:00

  • Transforming activity of the E6 gene of HPV-11gt in NIH 3T3 and REF 52 cells: correlation with the level of E6 transcription.

    abstract::Among human papillomavirus (HPV) types, clinical association with benign vs malignant lesions correlates with the ability of the corresponding oncogenes to transform cells in vitro. However, even though HPV-11 is considered a low-risk type, we have reported previously that the E5a oncogene of HPV-11gt is capable of tr...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1996.0279

    authors: Trujillo JM,Mounts P

    更新日期:1996-06-01 00:00:00

  • Nucleotide sequence analysis of the S genomic segment of Prospect Hill virus: comparison with the prototype Hantavirus.

    abstract::The genomic S RNA segment of Prospect Hill virus (PHV), a member of the Hantavirus genus, was molecularly cloned and the nucleotide sequence of the cDNA determined. The PHV S RNA segment is 1675 nucleotides long. A long open reading frame was identified in the viral complementary-sense RNA that could encode a 433 amin...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(90)90197-y

    authors: Parrington MA,Kang CY

    更新日期:1990-03-01 00:00:00

  • Autographa californica M nuclear polyhedrosis virus: microtubules and replication.

    abstract::Progressive reorganization and depolymerization of microtubules corresponded with virus-induced rounding of Autographa californica M nuclear polyhedrosis virus (AcMNPV)-infected Spodoptera frugiperda IPLB-Sf-21 cells, suggesting that microtubules were instrumental in maintaining the normal shape of these cells. Depoly...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(90)90211-9

    authors: Volkman LE,Zaal KJ

    更新日期:1990-03-01 00:00:00

  • Differential growth of human enteric adenovirus 41 (TAK) in continuous cell lines.

    abstract::Differing reports exist about the replication of human enteric adenoviruses (EnAds) in various cell lines. There was a suggestion that EnAds are defective, do not grow on primary human diploid cells, and behave like Ad host-range mutants, i.e., they require early gene products from other Ad types for efficient growth....

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(90)90072-y

    authors: Pieniazek D,Pieniazek NJ,Macejak D,Coward J,Rayfield M,Luftig RB

    更新日期:1990-01-01 00:00:00

  • Strain variation in Epstein-Barr virus immediate early genes.

    abstract::RNase protection analysis of mRNAs for Epstein-Barr virus (EBV) genes activated early in the induction of the productive cycle in Akata cells has confirmed that BZLF1 and BRLF1 behave as immediate early genes. These are likely to be the only virus immediate early genes since analysis of three other transcripts previou...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1993.1070

    authors: Packham G,Brimmell M,Cook D,Sinclair AJ,Farrell PJ

    更新日期:1993-02-01 00:00:00

  • In vitro packaging of plasmid DNA oligomers by Salmonella phage P22: independence of the pac site, and evidence for the termination cut in vitro.

    abstract::In vitro packaging experiments with phage P22 using artificially ligated plasmid concatemers have shown that the pac site is not necessary for DNA packaging although in vivo this initiation signal is indispensable. This indicates that the phage-coded protein gp3 also executes other important functions during phage mat...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(85)90273-9

    authors: Behnisch W,Schmieger H

    更新日期:1985-07-30 00:00:00

  • Detection of subgenomic cDNAs and mapping of feline foamy virus mRNAs reveals complex patterns of transcription.

    abstract::Feline foamy virus (FeFV) belongs to the group of spumaretroviruses that contain in addition to gag, pol, and env accessory genes collectively called bel genes. Primate FVs have been shown to utilize internal promoters in addition to the 5' LTR promoters. In contrast to other known retroviruses, the FV pol genes are e...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1998.9113

    authors: Bodem J,Löchelt M,Delius H,Flügel RM

    更新日期:1998-05-10 00:00:00

  • Construction and analysis of infectious transcripts synthesized from full-length cDNA clones of both genomic RNAs of pea early browning virus.

    abstract::Full-length cDNA clones of both RNAs of pea early browning virus have been constructed. Synthetic transcripts derived in vitro from these clones are infectious when inoculated onto plants. Electron microscopy revealed the presence of virions in transcript-inoculated plants, and both purified RNA and virions isolated f...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(91)90655-u

    authors: MacFarlane SA,Wallis CV,Taylor SC,Goulden MG,Wood KR,Davies JW

    更新日期:1991-05-01 00:00:00

  • Simian Immunodeficiency Virus seroreactivity in inhabitants from rural Cameroon frequently in contact with non-human primates.

    abstract::Central African tropical forests are home to several species of non-human primates (NHPs), infected by Simian Immunodeficiency Virus (SIV). It is well-known that HIV-1 epidemic is due to cross-transmission and adaptation of SIV to humans. The main goal of this work was to investigate if a NHP bite is a risk factor for...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2016.12.026

    authors: Filippone C,de Oliveira F,Betsem E,Schaeffer L,Fontanet A,Lemée V,Gessain A,Plantier JC

    更新日期:2017-03-01 00:00:00

  • Identification and characterization of a murine cytomegalovirus gene with homology to the UL25 open reading frame of human cytomegalovirus.

    abstract::Monoclonal antibody 1B4, previously shown to be protective in vivo and to cross-react with both virally encoded and normal host cell proteins, was used to screen a lambda gt11 cDNA derived from mRNA harvested from mouse embryo fibroblasts 24 hr after infection with murine cytomegalovirus (MCMV). A 700-bp cDNA was iden...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1994.1227

    authors: Dallas PB,Lyons PA,Hudson JB,Scalzo AA,Shellam GR

    更新日期:1994-05-01 00:00:00

  • Assembly of viroplasm and virus-like particles of rotavirus by a Semliki Forest virus replicon.

    abstract::In this study we have used an expression system based on Semliki Forest virus (SFV) to study assembly and intracellular localization of certain capsid proteins of rotavirus in neurons and mammalian epithelial cells. The complete genes of vp2 (vp2A) and vp6 (vp6A) of group A rotavirus (SA-11) and gene 5 encoding vp6 (v...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1997.8987

    authors: Nilsson M,von Bonsdorff CH,Weclewicz K,Cohen J,Svensson L

    更新日期:1998-03-15 00:00:00

  • Sequences of the N and M genes of the sigma virus of Drosophila and evolutionary comparison.

    abstract::The genome of the sigma rhabdovirus of Drosophila melanogaster consists of six genes in the order 3' N-2-3-4-G-L 5'. The nucleotide sequences of the N and of the fourth genes were determined from cDNA clones. Each gene contained a single long open reading frame encoding polypeptides with predicted MW of 50 and 25 kDa,...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1994.1177

    authors: Bras F,Teninges D,Dezelee S

    更新日期:1994-04-01 00:00:00

  • The signal-anchor domain of adenovirus E3-6.7K, a type III integral membrane protein, can direct adenovirus E3-gp19K, a type I integral membrane protein, into the membrane of the endoplasmic reticulum.

    abstract::Type III integral membrane proteins are oriented in the membrane with their C-terminus in the cytoplasm and their N-terminus extracytoplasmic. Such proteins are believed to have an internal hydrophobic sequence that functions both as an uncleaved signal for membrane insertion and also to anchor the protein in the memb...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1994.1266

    authors: Wilson-Rawls J,Deutscher SL,Wold WS

    更新日期:1994-05-15 00:00:00

  • The gag and pol genes of bovine leukemia virus: nucleotide sequence and analysis.

    abstract::The DNA sequence of the gag and pol regions of a provirus cloned from a bovine tumor is presented. In order to confirm these results the sequence of portions of a second clone, derived from a virus-producing cell line, was also determined. The gag gene was found to consist of 1179 nucleotides, which probably encode on...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/0042-6822(85)90344-7

    authors: Rice NR,Stephens RM,Burny A,Gilden RV

    更新日期:1985-04-30 00:00:00

  • Bacteriophage P4 capsid-size determination and its relationship to P2 helper interference.

    abstract::The sid (size determination) gene product of phage P4 is known to be involved in capsid-size determination. Moreover, the capsid-size determination function interferes with the lytic development of its helper P2, presumably because the helper genome is too large to be packaged into P4-size capsids. In order to study P...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1006/viro.1996.0270

    authors: Nilssen O,Fossdal CG,Johansen BV,Lindqvist BH

    更新日期:1996-05-15 00:00:00

  • The retroviral cyclin of walleye dermal sarcoma virus binds cyclin-dependent kinases 3 and 8.

    abstract::Walleye dermal sarcoma virus encodes a retroviral cyclin (rv-cyclin) with a cyclin box fold and transcription activation domain (AD). Co-immune precipitation (co-IP) identified an association of rv-cyclin with cyclin-dependent kinase 8 (cdk8). Cdk8 is dependent upon cyclin C and regulates transcription with the Mediat...

    journal_title:Virology

    pub_type: 杂志文章

    doi:10.1016/j.virol.2010.10.022

    authors: Brewster CD,Birkenheuer CH,Vogt MB,Quackenbush SL,Rovnak J

    更新日期:2011-01-20 00:00:00