当前位置： SCI文献检索 > VIROLOGY期刊下所有文献 > The gag and pol genes of bovine leukemia virus: nucleotide sequence and analysis.

The gag and pol genes of bovine leukemia virus: nucleotide sequence and analysis.

Abstract：

:The DNA sequence of the gag and pol regions of a provirus cloned from a bovine tumor is presented. In order to confirm these results the sequence of portions of a second clone, derived from a virus-producing cell line, was also determined. The gag gene was found to consist of 1179 nucleotides, which probably encode only three proteins: an N-terminal protein of 109 amino acids, a major core protein (p24) of 215 amino acids, and a nucleic acid binding protein (p12) of 69 residues. An open reading frame, whose translated product showed clear homology to the avian and murine proteases, was found beginning immediately upstream of the 3' end of gag. Following this protease region, a third long open reading frame, encoding 852 amino acids, showed clear homology to both avian and murine pol genes. The mechanism of translation of the protease and pol gene products cannot be predicted with certainty. Like Moloney murine leukemia virus (M-MuLV), BLV has a termination signal at the 3' end of gag, but unlike M-MuLV the protease is in a different reading frame. Like Rous sarcoma virus (RSV), BLV has a termination signal at the 3' end of the protease region and the reverse transcriptase is in a different (i.e., the third) reading frame. Possible translation mechanisms are discussed. Finally, the BLV gag and pol gene products are highly related to those of the human T-cell leukemia virus (HTLV); relatedness varied from 37% amino acid identities within the N terminal gag protein to 54% within the nucleic acid binding protein. Highly significant homology with both murine and avian type-C proteins was found within p24, p12, and the putative protease, reverse transcriptase, and endonuclease. Based on this homology, the BLV-HTLV family of viruses appears about equally distantly related to murine and avian type-C viruses.

journal_name

Virology

journal_title

Virology

authors

Rice NR,Stephens RM,Burny A,Gilden RV

doi

10.1016/0042-6822(85)90344-7

subject

Has Abstract

pub_date

1985-04-30 00:00:00

pages

357-77

issue

2

eissn

0042-6822

issn

1096-0341

journal_volume

142

pub_type

杂志文章

相关文献

VIROLOGY文献大全
  • Geographic distribution and evolution of Ross River virus in Australia and the Pacific Islands.

    abstract：:We examined the molecular epidemiology and evolution of Ross River (RR) virus in Australia and the Pacific Islands. Nucleotide sequences of the E2 and E3 genes of five RR virus strains revealed remarkable conservation between 1959 and 1989 with a maximum divergence of only 3.3%. Sequence data from a 505-base pair frag...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1995.1449

    authors： Sammels LM,Coelen RJ,Lindsay MD,Mackenzie JS

    更新日期：1995-09-10 00:00:00

  • Localization of two protease-sensitive regions separating distinct domains in the reovirus cell-attachment protein sigma 1.

    abstract：:Electron microscopy and sequence analysis have suggested the presence of distinct morphological regions within the reovirus cell attachment protein sigma 1. Kinking of purified sigma 1 observed by electron microscopy and sequence-predicted flexibility profiles suggest the presence of potential flexible regions in the ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1994.1465

    authors： Duncan R,Lee PW

    更新日期：1994-08-15 00:00:00

  • Defining HIV-1 Vif residues that interact with CBFβ by site-directed mutagenesis.

    abstract：:Vif is essential for HIV-1 replication in T cells and macrophages. Vif recruits a host ubiquitin ligase complex to promote proteasomal degradation of the APOBEC3 restriction factors by poly-ubiquitination. The cellular transcription cofactor CBFβ is required for Vif function by stabilizing the Vif protein and promotin...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2013.11.004

    authors： Matsui Y,Shindo K,Nagata K,Io K,Tada K,Iwai F,Kobayashi M,Kadowaki N,Harris RS,Takaori-Kondo A

    更新日期：2014-01-20 00:00:00

  • Tomato spotted wilt Tospovirus genome reassortment and genome segment-specific adaptation.

    abstract：:A system to associate specific genome segments with viral phenotypes and to study factors influencing genome reassortment was developed for tomato spotted wilt Tospovirus (TSWV). Reassortant isolates were generated by co-inoculating a TSWV isolate, TSWV-D, with TSWV-10 or TSWV-MD. The parental origin of each genome se...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9131

    authors： Qiu WP,Geske SM,Hickey CM,Moyer JW

    更新日期：1998-04-25 00:00:00

  • Molecular cloning of a feline leukemia provirus integrated adjacent to the c-myc gene in a feline T-cell leukemia cell line and the unique structure of its long terminal repeat.

    abstract：:This paper reports the molecular cloning of a rearranged c-myc region from the FT-1 cell line, which was derived from a spontaneous feline T-cell leukemia carrying the feline leukemia virus (FeLV). An abnormal c-myc EcoRI fragment of about 18 kilobases, detected by Southern blotting, was molecularly cloned from the DN...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90172-4

    authors： Miura T,Shibuya M,Tsujimoto H,Fukasawa M,Hayami M

    更新日期：1989-04-01 00:00:00

  • Analysis of the phosphorylation status of Epstein-Barr virus LMP2A in epithelial cells.

    abstract：:LMP2A deletion and point mutants, with mutations in phosphotyrosine-containing protein-protein interaction motifs, were transiently expressed in 293 cells and their phosphorylation was examined in immune complex kinase assays as well as in vivo. In vitro LMP2A phosphorylation depended on tyrosine 112. In vivo, mutatio...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.2001.1197

    authors： Scholle F,Longnecker R,Raab-Traub N

    更新日期：2001-12-20 00:00:00

  • Adenovirus infection inactivates the translational inhibitors 4E-BP1 and 4E-BP2.

    abstract：:Infection with many viruses results in the selective shutoff of host protein synthesis. A common target for virus interference with host protein synthesis is the cap-binding protein complex, eIF4F. The large subunit of the complex, eIF4G, is cleaved upon picornavirus (except cardiovirus) infection. Infection with aden...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8757

    authors： Gingras AC,Sonenberg N

    更新日期：1997-10-13 00:00:00

  • In Nicotiana species, an artificial microRNA corresponding to the virulence modulating region of Potato spindle tuber viroid directs RNA silencing of a soluble inorganic pyrophosphatase gene and the development of abnormal phenotypes.

    abstract：:Potato spindle tuber viroid (PSTVd) is a small non-protein-coding RNA pathogen that can induce disease symptoms in a variety of plant species. How PSTVd induces disease symptoms is a long standing question. It has been suggested that PSTVd-derived small RNAs (sRNAs) could direct RNA silencing of a targeted host gene(s...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2013.12.019

    authors： Eamens AL,Smith NA,Dennis ES,Wassenegger M,Wang MB

    更新日期：2014-02-01 00:00:00

  • Molecular cloning of a novel human papillomarvirus (type 58) from an invasive cervical carcinoma.

    abstract：:A novel human papillomavirus type (HPV) was cloned from an invasive cervical carcinoma. The viral clone showed no homology with other known prototypes of HPV (HPV-1 through HPV-57), except HPV-33 by Southern blot analysis under stringent conditions. It showed less than 20% homology to HPV-33 by reassociation kinetic a...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90560-e

    authors： Matsukura T,Sugase M

    更新日期：1990-08-01 00:00:00

  • Changing sensitivity to broadly neutralizing antibodies b12, 2G12, 2F5, and 4E10 of primary subtype B human immunodeficiency virus type 1 variants in the natural course of infection.

    abstract：:The conserved nature of the epitopes of the four broadly neutralizing antibodies (BNAbs), b12, 2G12, 2F5, and 4E10, may imply that the sensitivity of HIV-1 for these BNAbs remains fairly constant over the course of infection. Here, we demonstrate that viruses isolated early during the course of infection were mostly s...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.05.028

    authors： Bunnik EM,van Gils MJ,Lobbrecht MS,Pisas L,van Nuenen AC,Schuitemaker H

    更新日期：2009-08-01 00:00:00

  • In vitro selection and characterisation of influenza B/Beijing/1/87 isolates with altered susceptibility to zanamivir.

    abstract：:We describe the in vitro selection and characterisation of virus derived from B/Beijing/1/87 passaged in the presence of zanamivir. During zanamivir passage, the phenotype of virus isolates was either drug dependent or drug resistant in plaque reduction assays. The susceptibility of the neuraminidase of the drug-depen...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1999.0058

    authors： Barnett JM,Cadman A,Burrell FM,Madar SH,Lewis AP,Tisdale M,Bethell R

    更新日期：1999-12-20 00:00:00

  • Functional analysis of bipartite begomovirus coat protein promoter sequences.

    abstract：:We demonstrate that the AL2 gene of Cabbage leaf curl virus (CaLCuV) activates the CP promoter in mesophyll and acts to derepress the promoter in vascular tissue, similar to that observed for Tomato golden mosaic virus (TGMV). Binding studies indicate that sequences mediating repression and activation of the TGMV and ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.03.012

    authors： Lacatus G,Sunter G

    更新日期：2008-06-20 00:00:00

  • First field isolation of wound tumor virus from a plant host: minimal sequence divergence from the type strain isolated from an insect vector.

    abstract：:A new strain of wound tumor virus (WTV) has been isolated from a periwinkle plant (Catharanthus roseus) that was among several used as bait plants in a blueberry field. The 12 segments of double-stranded RNA of the viral genome were isolated directly from infected tissue and found to have mobilities through agarose ge...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(91)90568-v

    authors： Hillman BI,Anzola JV,Halpern BT,Cavileer TD,Nuss DL

    更新日期：1991-12-01 00:00:00

  • Mu-opioid modulation of HIV-1 coreceptor expression and HIV-1 replication.

    abstract：:A substantial proportion of HIV-1-infected individuals are intravenous drug users (i.v.DUs) who abuse opiates. Opioids induce a number of immunomodulatory effects that may directly influence HIV-1 disease progression. In the present report, we have investigated the effect of opioids on the expression of the major HIV-...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/s0042-6822(03)00015-1

    authors： Steele AD,Henderson EE,Rogers TJ

    更新日期：2003-04-25 00:00:00

  • Identification of a MMTV insertion mutation within the coding region of the Fgf-3 protooncogene.

    abstract：:The Fgf-3 protooncogene (previously called int-2) is a target of proviral insertion mutations in mammary tumors induced by the mouse mammary tumor virus (MMTV). These insertion mutations result in the transcriptional activation of Fgf-3, which is not normally expressed in the adult mammary gland. Previous mapping stud...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1997.8794

    authors： Morris DW,Dutra JC

    更新日期：1997-11-10 00:00:00

  • Virion-associated viral fibroblast growth factor stimulates cell motility.

    abstract：:The Autographa californica M nucleopolyhedrovirus (AcMNPV) viral fibroblast growth factor (vFGF) has functional parallels to cellular FGFs. Deletion of the AcMNPV vfgf has no obvious phenotype in cell culture but delays the time of insect death. Here, we determined vFGF production during virus infection. vFGF was dete...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2009.09.011

    authors： Lehiy CJ,Martinez O,Passarelli AL

    更新日期：2009-12-05 00:00:00

  • Foot-and-mouth disease virus (FMDV) with a stable FLAG epitope in the VP1 G-H loop as a new tool for studying FMDV pathogenesis.

    abstract：:Foot-and-mouth disease virus (FMDV) VP1 G-H loop contains the major antigenic site. By replacing the sequence upstream of the RGD motif with a FLAG epitope, a marker virus for pathogenesis studies was generated. In cell culture, the recombinant virus containing FLAG (A24-FLAG) exhibited similar plaque phenotypes and g...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2012.11.001

    authors： Lawrence P,Pacheco JM,Uddowla S,Hollister J,Kotecha A,Fry E,Rieder E

    更新日期：2013-02-05 00:00:00

  • Analysis of the site occupancy constraints of primary amino acid sequences in the motif directing palmitylation of the vaccinia virus 37-kDa envelope protein.

    abstract：:Vaccinia virus (VV) encodes a 37-kDa envelope protein (p37) that is palmitylated on cysteine residues 185186 of the 372-amino acid protein. We have previously reported on a loosely conserved consensus motif. Further analysis has identified a conserved consensus sequence, Hydro*AAC(C)A (Hydro* represents a hydrophobic ...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1006/viro.1998.9543

    authors： Hansen SG,Grosenbach DW,Hruby DE

    更新日期：1999-02-01 00:00:00

  • Partial nucleotide sequence of St. Louis encephalitis virus RNA: structural proteins, NS1, ns2a, and ns2b.

    abstract：:cDNA clones of the St. Louis encephalitis (SLE) virus genome have been obtained and the nucleotide sequence of 4.7 kb corresponding to the 5' terminal half of the genome determined. The genome contains a 5' noncoding region of 98 nucleotides followed by a single continuous open reading frame that encodes three structu...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(87)90409-0

    authors： Trent DW,Kinney RM,Johnson BJ,Vorndam AV,Grant JA,Deubel V,Rice CM,Hahn C

    更新日期：1987-02-01 00:00:00

  • Restriction of Moloney murine leukemia virus replication in Moloney murine sarcoma virus-infected cells.

    abstract：:MuSV349 is a TB-cell line which produces infectious MuSV with little or no MuLV detectable by the XC assay. The apparent restriction of MuLV replication in MuSV349 cells was investigated. A replication-competent helper virus, with protein composition nearly identical to that of Mo-MuLV was isolated from the viruses pr...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(84)90043-6

    authors： Yuen PH,Soong MM,Kissil MS,Wong PK

    更新日期：1984-01-30 00:00:00

  • Herpes simplex virus type 1 tegument proteins VP1/2 and UL37 are associated with intranuclear capsids.

    abstract：:The assembly of the tegument of herpes simplex virus type 1 (HSV-1) is a complex process that involves a number of events at various sites within virus-infected cells. Our studies focused on determining whether tegument proteins, VP1/2 and UL37, are added to capsids located within the nucleus. Capsids were isolated fr...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.11.031

    authors： Bucks MA,O'Regan KJ,Murphy MA,Wills JW,Courtney RJ

    更新日期：2007-05-10 00:00:00

  • Molecular cloning of a novel human papillomavirus (type 60) from a plantar cyst with characteristic pathological changes.

    abstract：:In a plantar cyst composed of the wall of the squamous cell layer and the horny inner substance in the lower dermis, we found characteristic pathological changes, such as cytoplasmic eosinophilic inclusions and vacuolated structure, and, immunohistochemically, the papillomavirus capsid antigen. The human papillomaviru...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(92)91254-r

    authors： Matsukura T,Iwasaki T,Kawashima M

    更新日期：1992-09-01 00:00:00

  • Effect of exogenous interferon and an interferon inducer on western equine encephalitis virus disease in a hamster model.

    abstract：:Mice are used as models for western equine encephalitis virus (WEEV) infection, but high mortality is generally only seen with intracranial or intranasal challenge, while peripheral inoculation results in approximately 50% mortality and is not dose-dependent. Hamsters were therefore studied as a model for WEEV infecti...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.10.031

    authors： Julander JG,Siddharthan V,Blatt LM,Schafer K,Sidwell RW,Morrey JD

    更新日期：2007-04-10 00:00:00

  • Development of Bean pod mottle virus-based vectors for stable protein expression and sequence-specific virus-induced gene silencing in soybean.

    abstract：:Plant virus-based vectors provide valuable tools for expression of foreign proteins in plants and for gene function studies. None of the presently available virus vectors is suitable for use in soybean. In the present study, we produced Bean pod mottle virus (BPMV)-based vectors that are appropriate for gene expressio...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2005.08.046

    authors： Zhang C,Ghabrial SA

    更新日期：2006-01-20 00:00:00

  • Immunogenicity of a plant-derived edible rotavirus subunit vaccine transformed over fifty generations.

    abstract：:Major efforts have been put forth for the development of effective rotavirus vaccines including transgenic plant vaccines. Previous studies have reported that rotavirus VP7 maintains its neutralizing immunity when it is transformed into the potato genome. The present study was aimed at investigating the hereditary sta...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2006.07.045

    authors： Li JT,Fei L,Mou ZR,Wei J,Tang Y,He HY,Wang L,Wu YZ

    更新日期：2006-12-05 00:00:00

  • Selection for gene junction sequences important for VSV transcription.

    abstract：:The heptauridine tract at each gene end and intergenic region (IGR) at the gene junctions of vesicular stomatitis virus (VSV) have effects on synthesis of the downstream mRNA, independent of their respective roles in termination of the upstream mRNA. To investigate the role of the U tract and the IGR in downstream gen...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2008.08.001

    authors： Hinzman EE,Barr JN,Wertz GW

    更新日期：2008-10-25 00:00:00

  • Vaccinia virus induces cell fusion at acid pH and this activity is mediated by the N-terminus of the 14-kDa virus envelope protein.

    abstract：:The mechanism by which the large-size poxviruses enter animal cells is not known. In this investigation we show that acid pH treatment of wild-type vaccinia virus-infected cells triggers strong fusion of cells in culture, with an optimum at pH 4.8. We have identified the virus-induced fusion protein as a 14-kDa envelo...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90381-z

    authors： Gong SC,Lai CF,Esteban M

    更新日期：1990-09-01 00:00:00

  • An insertion mutation in the pol gene of Moloney murine leukemia virus results in temperature-sensitive pol maturation and viral replication.

    abstract：:An insertion mutation in the pol gene of Moloney murine leukemia virus (M-MuLV) was found to render the virus temperature-sensitive for replication. A provirus containing a 12-bp insertion at the boundary between the reverse transcriptase (RT) and integrase (IN) domains induced the formation of mutant virions containi...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(89)90428-5

    authors： Tanese N,Roth M,Epstein H,Goff SP

    更新日期：1989-06-01 00:00:00

  • Nucleolin promotes in vitro translation of feline calicivirus genomic RNA.

    abstract：:Feline calicivirus depends on host-cell proteins for its replication. We previously showed that knockdown of nucleolin (NCL), a phosphoprotein involved in ribosome biogenesis, resulted in the reduction of FCV protein synthesis and virus yield. Here, we found that NCL may not be involved in FCV binding and entry into c...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/j.virol.2015.12.001

    authors： Hernández BA,Sandoval-Jaime C,Sosnovtsev SV,Green KY,Gutiérrez-Escolano AL

    更新日期：2016-02-01 00:00:00

  • Replication of minute virus of mice DNA in adenovirus-infected or adenovirus-transformed cells.

    abstract：:The effect of adenovirus infection or transformation on the DNA replication of Minute Virus of Mice (MVM) was studied in human fibroblast cell lines. In WI38, HeLa, and 293 cells MVM infection allowed production of viral NS-1 and capsid proteins with or without adenovirus 2 (Ad2) co-infection. However, MVM DNA replica...

    journal_title：Virology

    pub_type： 杂志文章

    doi：10.1016/0042-6822(90)90010-o

    authors： Fox E,Moen PT Jr,Bodnar JW

    更新日期：1990-06-01 00:00:00