Abstract:
:LMP2A deletion and point mutants, with mutations in phosphotyrosine-containing protein-protein interaction motifs, were transiently expressed in 293 cells and their phosphorylation was examined in immune complex kinase assays as well as in vivo. In vitro LMP2A phosphorylation depended on tyrosine 112. In vivo, mutations of single tyrosines did not eliminate LMP2 phosphorylation, although mutation of the LMP2A ITAM decreased LMP2A phosphorylation. The relationship between LMP2A in vitro phosphorylation and that induced by cell-extracellular matrix (ECM) interactions was also investigated. While LMP2A was phosphorylated to higher levels in whole-cell extracts of stimulated cells, a difference in in vitro kinase assays with extracts from stimulated and unstimulated cells was not detected, indicating that the ECM-mediated regulation of LMP2A phosphorylation is lost in vitro. In the presence of LMP2A, several cellular proteins with molecular weights between 70 and 80 kDa were phosphorylated on tyrosine. This increase in cellular protein phosphorylation depended on the LMP2A ITAM motif and suggests that the ITAM may participate in signal-transduction events in epithelial cells.
journal_name
Virologyjournal_title
Virologyauthors
Scholle F,Longnecker R,Raab-Traub Ndoi
10.1006/viro.2001.1197subject
Has Abstractpub_date
2001-12-20 00:00:00pages
208-14issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(01)91197-3journal_volume
291pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::The primary cell types that sustain polyomavirus (Py) replication following intranasal infection as well as the nature of the host cellular response to Py were unknown. As this is an essential and specific site for virus entry, it seems likely that viral gene function must be adapted to these mucosal tissues. Using im...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.0030
更新日期:2000-01-05 00:00:00
abstract::Vaccinia topoisomerase provides a model system for structure-function analysis of the type IB topoisomerase family. Here we performed an alanine scan of eight positions in the beta4 and beta5 strands of the N-terminal domain (Leu57, Ile58, Phe59, Val60, Gly61, Ser62, Gln69 and Gly73) and eight positions in the alpha8-...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.08.056
更新日期:2007-03-15 00:00:00
abstract::Chlorella viruses or chloroviruses form polysaccharide fibers on the cell wall of host Chlorella cells after infection. Such polysaccharides are either hyaluronan synthesized by virus-encoded hyaluronan synthase (HAS) or chitin synthesized by viral chitin synthase (CHS). Some chloroviruses synthesize both hyaluronan (...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.07.026
更新日期:2005-11-10 00:00:00
abstract::Although herpesviruses have a wide host range and their genomes vary substantially in size, the nucleocapsid appears to be a conservative element of viral design. The capsid shell is icosahedrally symmetric (T = 16), and 125 nm in diameter and 15nm thick in the case of herpes simplex virus 1 (HSV-1). Channel catfish v...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0016
更新日期:1996-01-15 00:00:00
abstract::The differential symptom determinants of the Holmes' masked (M) and U1 strains of tobacco mosaic virus previously were mapped to the 5'-coterminal open reading frame (ORF) encoding the 126-kDa protein and the N-terminal two-thirds of the 183-kDa protein. Both proteins influence viral RNA accumulation, but the function...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0368
更新日期:1996-07-01 00:00:00
abstract::A chloroplast-localized papaya methionine sulfoxide reductase B1 (PaMsrB1) interacting with Papaya ringspot virus (PRSV) NIa-Pro was identified using a Sos recruitment two-hybrid system (SRS). SRS analysis of several deletion mutants of PRSV NIa-Pro and PaMsrB1 demonstrated that the C-terminal (residues 133-239) fragm...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2012.09.007
更新日期:2012-12-05 00:00:00
abstract::To determine whether infection with HIV-1 strains of different tropisms would influence expression of the mucosa-associated integrins alpha 4 beta 7 and alpha E beta 7 or the lymph node homing receptor L-selectin on peripheral T lymphocytes, cells were infected with the CXCR4-tropic (X4)/syncytium-inducing (SI) HIV-1(...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2000.0781
更新日期:2001-02-15 00:00:00
abstract::There is growing evidence that the receptor-binding characteristics of influenza viruses are affected by the host-dependent glycosylation of viral hemagglutinin (HA). To better understand these effects, we propagated two variants of the human influenza virus USSR/90/77 (which differed by the mutation Asn131 reversible...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9224
更新日期:1998-08-01 00:00:00
abstract::The DNAs of the Cas-Br-E MuLV-induced leukemias always contain somatically acquired mink cell focus-forming (MCF) recombinant proviruses. MCF recombinants could be involved during leukemogenesis at both preleukemic times and in late-stage tumors. Among the Cas-Br-E-induced non-T-, non-B-cell leukemias, viral integrati...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90241-g
更新日期:1992-12-01 00:00:00
abstract::The replication of vaccinia virus was reduced by 3 logs in cells that had been treated before and during infection with a concentration of N-(phosphonoacetyl)-L-aspartate (PALA) which lowered the UTP and CTP to 5 and 20% of controls, respectively, without affecting cell viability. The antiviral activity of PALA was re...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8735
更新日期:1997-09-15 00:00:00
abstract::Mixed infection of nonpermissive bacteria by amber mutant and wild-type T4 phage reduces the burst size in a manner that depends on the nature of the gene product eliminated by the mutation. While the genotypic proportions produced by such mixedly infected cells were directly proportional to those in the starting mixt...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90011-4
更新日期:1984-08-01 00:00:00
abstract::The neuraminidase (NA) genes of influenza B viruses B/Maryland/59, B/Hong Kong/8/73, B/Singapore/222/79, B/Oregon/5/80, B/USSR/100/83, B/Victoria/3/85, B/Leningrad/179/86, B/Memphis/6/86, and B/Memphis/3/89 have been sequenced. The deduced amino acid sequences show high variability in the stalk domain of the NA, but a...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90523-t
更新日期:1990-08-01 00:00:00
abstract::Previously our laboratory constructed an HIV-1 which stably maintained a primer binding site (PBS) complementary to tRNA(His) by mutating the region of the provirus within U5 postulated to interact with the anticodon of tRNA(His) (J. Wakefield, S-M Kang, and C. D. Morrow, 1996, J. Virol, 70, 966-975). From the analysi...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8837
更新日期:1997-11-24 00:00:00
abstract::We have previously shown that the late promoter of the human papovavirus BK (prototype) is contained within the three 68-bp repeats and a 66-bp region to the late side of the repeats which together constitute the early promoter enhancer. We have now carried out unidirectional deletion and linker scan analyses of these...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90053-6
更新日期:1989-03-01 00:00:00
abstract::Tobacco (Nicotiana edwardsonii and N. benthamiana) protoplasts infected with the plant rhabdovirus sonchus yellow net virus (SYNV) were found to be suitable for studies of replication. SYNV messenger RNAs could be detected within 2 hr postinoculation (PI), accumulated to a maximum within 24 hr, and subsequently declin...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90149-l
更新日期:1990-12-01 00:00:00
abstract::Long-term persistent infections were established with the flavivirus, West Nile virus (WNV), strain E101, in embryofibroblast cultures derived from susceptible C3H/HE and congenic-resistant C3H/RV mice. Cultures were initially maintained by weekly subculture at 37 degrees, but at passage 6 sister cultures were shifted...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90454-4
更新日期:1985-01-15 00:00:00
abstract::The parvovirus-encoded nonstructural (NS) proteins have been implicated in the cytopathogenicity of these agents. Although protein NS-1 of minute virus of mice (MVM) has been shown to be toxic, little is known about the role of NS-2 in this process. In order to determine the contribution of NS-1 and NS-2 to cytotoxici...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1993.1355
更新日期:1993-07-01 00:00:00
abstract::The genes of the RF strain of bovine rotavirus have been cloned into pBR 322 following the synthesis and hybridization of cDNA transcribed from both strands of in vitro polyadenylated genomic RNA. Cloned rotavirus DNAs were assigned to most of the 11 genomic RNA segments by Northern blot hybridization. The complete se...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90159-4
更新日期:1984-10-15 00:00:00
abstract::The molecular mechanism(s) by which human immunodeficiency virus (HIV-1) injures a T-cell line was studied. A pathological role for viral env proteins, which are inserted into the plasma membrane, has been previously demonstrated for HIV as well as other retroviruses which are cytopathic. We therefore initiated studie...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90232-2
更新日期:1988-03-01 00:00:00
abstract::In the absence of an effective vaccine against HIV, it is urgent to develop an effective alternative such as a microbicide. Single and repeated applications of MC1220 microbicide were evaluated in macaques. First, animals were given a single application of 0.5% or 1.5% MC1220-containing liposomal gel. A second group w...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2010.06.008
更新日期:2010-09-15 00:00:00
abstract::Camelpox virus (CMLV), a member of the Orthopoxvirus genus in the Poxviridae, is the etiologic agent of a disease of camels. Here we report the CMLV genomic sequence with analysis. The 205,719-bp CMLV genome contains 211 putative genes and consists of a central region bound by identical inverted terminal repeats of ap...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.2001.1343
更新日期:2002-03-30 00:00:00
abstract::The sequence of gene VI from figwort mosaic virus (FMV) clone x4 was determined and compared with that previously published for FMV clone DxS. Both clones originated from the same virus isolation, but the virus used to clone DxS was propagated extensively in a host of a different family prior to cloning whereas that u...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90624-k
更新日期:1991-06-01 00:00:00
abstract::The genome of the lytic Pseudomonas putida bacteriophage gh-1 is linear double-stranded DNA containing 37,359 bp with 216-bp direct terminal repeats. Like other members of the T7 group, the gh-1 genome contains regions of high homology to T7 interspersed with nonhomologous regions that contain small open reading frame...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/s0042-6822(03)00124-7
更新日期:2003-07-05 00:00:00
abstract::Human cytomegalovirus U(L)112-113 encodes four phosphoproteins, pp84, pp50, pp43, and pp34, with common amino-termini. A previous report by Kerry et al. (J. Virol. 70, 373-382, 1996) demonstrated that U(L)112-113 products activate U(L)54 promoter in cooperation with immediate-early (IE) proteins. In this study, we ide...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1999.9800
更新日期:1999-07-20 00:00:00
abstract::Cells that express the human papillomavirus (HPV) type 16 E7 oncoprotein are predisposed to undergo apoptosis. Transgenic mice that have E7 expression targeted to either the retinal photoreceptor cells or the lens cells exhibit signs of apoptosis in cells attempting to undergo differentiation. We established a cell cu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8851
更新日期:1997-12-08 00:00:00
abstract::We have constructed a recombinant baculovirus expressing high levels of the herpes simplex virus type 1 (HSV-1) glycoprotein E (gE) in Sf9 cells. The expressed gE migrated on gels as a double band with apparent molecular weights of 68 and 70 kDa. The recombinant gE was glycosylated based on its susceptibility to tunic...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90500-o
更新日期:1992-06-01 00:00:00
abstract::The physical stability of virions of Cucumber mosaic virus (CMV) mutants was investigated to determine if relative stability correlated with efficiency of aphid transmission. Virion stability was evaluated by a urea disruption assay and by testing the infectivity of virus following purifications. All viruses were infe...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2004.11.021
更新日期:2005-02-05 00:00:00
abstract::Turnip crinkle carmovirus (TCV) has a uniquely high recombination frequency and nonrandom cross-over site distribution among the recombining TCV-associated satellite RNAs. An in vitro system has been developed that includes a partially purified TCV replicase preparation (RdRp) and chimeric RNAs that resemble the putat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9342
更新日期:1998-09-30 00:00:00
abstract::The human cytomegalovirus (HCMV) US12 gene family is a group of 10 predicted seven-transmembrane domain proteins that have some features in common with G-protein-coupled receptors. Little is known of their patterns of expression, localization, or functional interactions. Here, we studied the intracellular localization...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.11.019
更新日期:2007-05-10 00:00:00
abstract::Seven envelope regions from two series of patient isolates have been molecularly cloned and analyzed for replication phenotypes and immunogenicity. Growth potential was analyzed for env sequences substituted into an HIV-1-NL4-3 backbone (NL4-3/env recombinants). Immunogenicity studies were conducted on secreted monome...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8445
更新日期:1997-03-03 00:00:00