Abstract:
:Vaccinia topoisomerase provides a model system for structure-function analysis of the type IB topoisomerase family. Here we performed an alanine scan of eight positions in the beta4 and beta5 strands of the N-terminal domain (Leu57, Ile58, Phe59, Val60, Gly61, Ser62, Gln69 and Gly73) and eight positions in the alpha8-alpha9 loop of the C-terminal catalytic domain (Ser241, Ile242, Ser243, Pro244, Leu245, Pro246, Ser247, and Pro248). Mutants F59A, G73A, and Q69A displayed rate defects in relaxing supercoiled DNA that were attributed to effects on DNA binding rather than transesterification chemistry. Replacing Gln69 conservatively with Asn, Glu or Lys failed to restore relaxation activity. Gln69 is located along a concave DNA-binding surface of the N-terminal domain and it makes direct contact with the +2A base of the 5'-CCCTT/3-GGGAA target site for DNA cleavage. Gly73 is located at the junction between the N-terminal domain and catalytic domain and it is likely to act as a swivel for the large domain movements that coordinate DNA ingress and closure of the topoisomerase clamp around the duplex. Previous alanine scanning had identified Phe215 in helix alpha7 of the catalytic domain as contributing to DNA relaxation activity. Here we find that F215L resembles F215A in its diminished relaxation activity and its sensitivity to inhibition by salt. The Phe215 side chain makes van der Waals contacts to Ile98, Met121 and Phe101, which we propose stabilize a three helix bundle and promote clamp closure.
journal_name
Virologyjournal_title
Virologyauthors
Tian L,Shuman Sdoi
10.1016/j.virol.2006.08.056subject
Has Abstractpub_date
2007-03-15 00:00:00pages
466-76issue
2eissn
0042-6822issn
1096-0341pii
S0042-6822(06)00621-0journal_volume
359pub_type
杂志文章相关文献
VIROLOGY文献大全abstract::Phage ΦM12 is an important transducing phage of the nitrogen-fixing rhizobial bacterium Sinorhizobium meliloti. Here we report the genome, phylogenetic analysis, and proteome of ΦM12, the first report of the genome and proteome of a rhizobium-infecting T4-superfamily phage. The structural genes of ΦM12 are most simila...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.11.027
更新日期:2014-02-01 00:00:00
abstract::TMV capsid protein reacts with and encapsidates many of the chloroplast DNA transcripts both in vivo and in vitro to form pseudovirions. We report on the encapsidation initiation reaction with one of the major RNA species found in in vivo formed pseudovirions, the mRNA for the chloroplast-encoded large subunit of ribu...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(89)90280-8
更新日期:1989-02-01 00:00:00
abstract::Two temperature-sensitive mutants of herpes simplex virus type 1 in complementation group 1-1 were analyzed to determine if the major DNA-binding protein they produced was thermolabile. Cells infected with these mutants were analyzed for deoxyribonucleoprotein complexes containing the DNA-binding protein. These comple...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(84)90220-4
更新日期:1984-09-01 00:00:00
abstract::Twelve lytic viruses (PgV) infecting the marine unicellular eukaryotic harmful algal bloom species Phaeocystis globosa were isolated from the southern North Sea in 2000-2001 and partially characterized. All PgV isolates shared common phenotypic features with other algal viruses belonging to the family Phycodnaviridae ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2005.07.002
更新日期:2005-10-10 00:00:00
abstract::The role that interferon-gamma (IFNgamma) plays during herpetic stromal keratitis (HSK) has not been definitively determined. In primary HSK most reports suggest that IFNgamma may help control viral replication and contribute to corneal pathology. However, its role in recurrent HSK has not been directly addressed. The...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.07.018
更新日期:2008-10-10 00:00:00
abstract::The hepatitis B virus (HBV) is formed by budding. A stretch of 22 amino acids (aa) (matrix domain, MD, R103 - S124) in the large envelope protein L is crucial for virion formation and probably establishes contact to the nucleocapsid. Here, we assess the impact of sequence variations at numerous individual aa positions...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.04.030
更新日期:2014-06-01 00:00:00
abstract::KSHV establishes characteristic latent infections in vitro, while RRV, a related macaque rhadinovirus, establishes characteristic permissive infections with virus replication. We identified cells that are not permissive for RRV replication and recapitulate the latent KSHV infection and reactivation processes. The RRV ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2013.10.013
更新日期:2014-01-05 00:00:00
abstract::In this paper we present a technique developed to physically locate the HSV-1 gene(s) which restore neurovirulence to a non-neurovirulent HSV intertypic recombinant described in the preceding report. In brief, tissue culture cells are co-transfected with unit length RE6 DNA and restriction endonuclease fragmented HSV-...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90544-5
更新日期:1983-11-01 00:00:00
abstract::Little is known about the neutralization properties of HIV-1 in India to optimally design and test vaccines. For this reason, a functional Env clone was obtained from each of ten newly acquired, heterosexually transmitted HIV-1 infections in Pune, Maharashtra. These clones formed a phylogenetically distinct genetic li...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.12.032
更新日期:2009-03-15 00:00:00
abstract::In vitro packaging experiments with phage P22 using artificially ligated plasmid concatemers have shown that the pac site is not necessary for DNA packaging although in vivo this initiation signal is indispensable. This indicates that the phage-coded protein gp3 also executes other important functions during phage mat...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(85)90273-9
更新日期:1985-07-30 00:00:00
abstract::A system to associate specific genome segments with viral phenotypes and to study factors influencing genome reassortment was developed for tomato spotted wilt Tospovirus (TSWV). Reassortant isolates were generated by co-inoculating a TSWV isolate, TSWV-D, with TSWV-10 or TSWV-MD. The parental origin of each genome se...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1998.9131
更新日期:1998-04-25 00:00:00
abstract::We investigated the origin and the effect of insertion D67D-THGERDLGPA within HIV-1 RT from a patient failing antiviral therapy. The insertion developed within the context of pre-existing NRTI and NNRTI mutations (M41L, L210W, T215Y and N348I). Concurrently, the NRTI mutations T69I and V118I and the NNRTI mutations K1...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.03.023
更新日期:2014-05-01 00:00:00
abstract::The Sindbis virus mutant SB-RL, in contrast to its parent, Sindbis strain AR339 (SB), is attenuated in neonatal mice, has an increased rate of penetration in tissue culture cells, and is more sensitive to neutralization by E2-specific monoclonal antibodies (MCAbs) R6 and R13. These phenotypic differences are controlle...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90184-s
更新日期:1990-03-01 00:00:00
abstract::Astrocytes protect neurons but also evoke a proinflammatory response to injury and viral infections including HIV. We investigated the mechanism of HIV-1 infection in primary astrocytes, which showed minimal but productive viral infection independent of CXCR4. As with ectopic-CD4-expressing astrocytes, lysosomotropic ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2014.03.002
更新日期:2014-05-01 00:00:00
abstract::Gp110 of Epstein-Barr virus (EBV) mainly localizes on nuclear/ER membranes and plays a role in the assembly of EBV nucleocapsid. The C-terminal tail domain (gp110 CTD) is essential for the function of gp110 and the nuclear/ER membranes localization of gp110 is ruled by its C-terminal unique nuclear localization signal...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2008.06.031
更新日期:2008-09-30 00:00:00
abstract::The human papillomavirus type 16 E1--E4 protein is expressed abundantly in cells supporting viral DNA amplification, but its expression is lost during malignant progression. In cell culture, 16E1--E4 causes G2 cell cycle arrest by associating with and preventing the nuclear entry of Cdk1/cyclin B1 complexes. Here, we ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.02.024
更新日期:2006-05-25 00:00:00
abstract::The reticuloendotheliosis virus (REV) group of retroviruses and type D simian retroviruses (SRV) belong to the same receptor interference group. The cellular receptor for these viruses has not yet been identified. In order to study the distribution of the receptor and to identify a receptor negative cell line, vector ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1994.1651
更新日期:1994-11-15 00:00:00
abstract::The complete sequence of the genome of the cherry strain of tomato bushy stunt virus (TBSV), a member of the tombusvirus group, was determined. A full-length clone of the genome containing a bacteriophage T7 RNA polymerase promoter was assembled from partial cDNA clones. In vitro transcripts of the genome, either with...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(90)90468-7
更新日期:1990-07-01 00:00:00
abstract::A central, yet unresolved issue in the pathogenesis of HIV disease is the mechanism of antibody perturbation. In this study, HIV-specific memory B-cells were quantified in groups of infected subjects and compared with memory responses to other antigens and antibody titers. HIV-specific memory B-cell responses were vig...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2009.11.003
更新日期:2010-02-05 00:00:00
abstract::To define the number of rhinovirus serotypes, cross neutralization tests and characterization studies were completed on 25 candidate prototype rhinoviruses submitted to a third phase of a collaborative program. Based on the results, 11 distinct prototype strains were designated and the numbering system was extended to...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90367-9
更新日期:1987-07-01 00:00:00
abstract::Monoclonal antibodies (MAb) against an O1 Suisse isolate of FMDV were used to identify epitopes on the virus particle and to determine their relative function. Six major antigenic sites containing one or more epitopes were identified using competition ELISA. An epitope relationship is proposed consisting of a trypsin-...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(87)90294-7
更新日期:1987-04-01 00:00:00
abstract::Nineteen independent temperature-sensitive (ts) mutants were isolated from SA11 following mutagenesis with proflavin or 5-azacytidine. Fourteen of the ts mutants fell into one or another of five mutant groups previously defined by recombination. Five of the ts mutants defined five recombination groups (F, G, H, I, and...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(83)90100-9
更新日期:1983-10-30 00:00:00
abstract::An infectious raccoon poxvirus (RCNV) was used to express the feline panleukopenia virus (FPV) open reading frame VP2. The recombinant, RCNV/FPV, was constructed by homologous recombination with a chimeric plasmid for inserting the expression cassette into the thymidine kinase (TK) locus of RCNV. Expression of the VP2...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1996.0186
更新日期:1996-04-01 00:00:00
abstract::The nucleotide sequence of the portion of naturally occurring defective EBV (HR-1) DNA (designated het DNA) which is responsible for disruption of EBV latency has been determined and compared with the regions of the standard HR-1 viral genome from which it was derived. This rearranged 2.7-kbp DNA fragment represented ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(88)90599-5
更新日期:1988-08-01 00:00:00
abstract::In order to gain a better perspective on the true variability of varicella-zoster virus (VZV) and to catalogue the location and number of differences, 11 new complete genome sequences were compared with those previously in the public domain (18 complete genomes in total). Three of the newly sequenced genomes were deri...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2006.09.037
更新日期:2007-03-15 00:00:00
abstract::cDNA clones encoding the envelope glycoprotein E1 of rubella virus (RV) were altered by site-directed mutagenesis at consensus sites for addition of N-linked glycans. The resulting plasmids were introduced into COS cells and the mutant E1 proteins were analyzed by indirect immunofluorescence, radioimmunoprecipitation,...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(91)90915-x
更新日期:1991-04-01 00:00:00
abstract::The Fgf-3 protooncogene (previously called int-2) is a target of proviral insertion mutations in mammary tumors induced by the mouse mammary tumor virus (MMTV). These insertion mutations result in the transcriptional activation of Fgf-3, which is not normally expressed in the adult mammary gland. Previous mapping stud...
journal_title:Virology
pub_type: 杂志文章
doi:10.1006/viro.1997.8794
更新日期:1997-11-10 00:00:00
abstract::Enveloped virus entry occurs when viral and cellular membranes fuse releasing particle contents into the target cell. Human immunodeficiency virus (HIV) entry occurs by cell-free virus or virus transferred between infected and uninfected cells through structures called virological synapses. We developed a high-through...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2015.10.013
更新日期:2016-03-01 00:00:00
abstract::Expression of Kaposi's sarcoma herpesvirus vFLIP, a potent activator of NFkB signaling, promotes latency. Inhibition of NFkB signaling promotes lytic reactivation. We previously reported that lytic inducer, RTA, inhibits vFLIP induced NFkB signaling by inducing the degradation of vFLIP via the proteasome. Here we repo...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/j.virol.2016.11.016
更新日期:2017-01-15 00:00:00
abstract::Functions of the coat protein of white clover mosaic potexvirus (WCIMV) were investigated using C-terminal deletion mutants. Whereas plants inoculated with RNA transcripts of a full-length wild-type clone of WCIMV produced typical infections, plants inoculated with transcripts of each mutant did not produce symptoms, ...
journal_title:Virology
pub_type: 杂志文章
doi:10.1016/0042-6822(92)90215-b
更新日期:1992-11-01 00:00:00