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In vitro packaging of plasmid DNA oligomers by Salmonella phage P22: independence of the pac site, and evidence for the termination cut in vitro.

Abstract：

:In vitro packaging experiments with phage P22 using artificially ligated plasmid concatemers have shown that the pac site is not necessary for DNA packaging although in vivo this initiation signal is indispensable. This indicates that the phage-coded protein gp3 also executes other important functions during phage maturation in addition to the recognition of pac, or that its site specificity is lost in vitro. It has been shown previously that gp3 is necessary for in vitro packaging. Further, it was demonstrated that DNA which is only 74% of headful size cannot be packaged. Oversized DNA, however, is cut in vitro to unit length.

journal_name

Virology

journal_title

Virology

authors

Behnisch W,Schmieger H

doi

10.1016/0042-6822(85)90273-9

subject

Has Abstract

pub_date

1985-07-30 00:00:00

pages

310-7

issue

2

eissn

0042-6822

issn

1096-0341

journal_volume

144

pub_type

杂志文章

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