Interspecific reassortment of genomic segments in the evolution of cucumoviruses.

abstract：:The specificity of poliovirus encapsidation has been studied using a novel chimeric genome in which the gene encoding firefly luciferase has been substituted for the VP2-VP3-VP1 genes of the poliovirus capsid (P1) gene. Transfection of RNA transcribed in vitro from this genome resulted in a VP4-luciferase fusion prote...

journal_title：Virology

authors： Porter DC,Ansardi DC,Wang J,McPherson S,Moldoveanu Z,Morrow CD

更新日期：1998-03-30 00:00:00

abstract：:In addition to its role in virus assembly, the matrix (M) protein of vesicular stomatitis virus (VSV) is involved in virus-induced cell rounding and inhibition of host-directed gene expression. Previous experiments have shown that two M protein mutants genetically dissociate the ability of M protein to inhibit host-di...

journal_title：Virology

authors： Lyles DS,McKenzie MO

更新日期：1997-03-03 00:00:00

abstract：:Human adenovirus type 3 (HAdV-B3) has an apparently stable genome yet remains a major circulating and problematic respiratory pathogen. Comparisons of the prototype genome to genomes from three current field strains, including two isolated from epidemics, and a laboratory strain, yielded small-scale nucleotide variati...

journal_title：Virology

authors： Mahadevan P,Seto J,Tibbetts C,Seto D

更新日期：2010-02-05 00:00:00

abstract：:Hepatitis B viruses replicate via reverse transcription of an RNA intermediate. This RNA pregenome serves as mRNA and is packaged into capsids and reverse transcribed. Both processes require the interaction of the viral reverse transcriptase, P protein, with the 5'-proximal epsilon-signals on the pregenome. For epsilo...

journal_title：Virology

authors： Beck J,Bartos H,Nassal M

更新日期：1997-01-20 00:00:00

abstract：:The 50-70 S RNA complex of a clone of Kirsten murine leukemia virus (Ki-MLV), propagated in NIH 3T3 cells, as well as that of a Moloney (Mo)-MLV, propagated in a mouse thymus-bone marrow cell line, contained an approximately 30 S RNA of unknown biological activity in addition to 38 S genomic RNA. The approximately 30 ...

journal_title：Virology

authors： Duesberg PH,Scolnick EM

更新日期：1977-11-01 00:00:00

abstract：:At least four hantavirus species cause disease with prominent renal involvement-hemorrhagic fever with renal syndrome (HFRS); and several hantavirus strains cause disease with significant pulmonary involvement-hantavirus pulmonary syndrome (HPS). The most prevalent and lethal hantaviruses associated with HFRS and HPS ...

journal_title：Virology

authors： Hooper JW,Custer DM,Smith J,Wahl-Jensen V

更新日期：2006-03-30 00:00:00

abstract：:Infection of Salmonella enterica with lytic mutants of either P22 or SE1 bacteriophages triggers the expression of its DNA damage-inducible SOS response through a lexA-dependent pathway. This induction of the SOS system strictly requires the presence of the bacteriophage kil gene. Accordingly, plasmid overexpression o...

journal_title：Virology

authors： Campoy S,Hervàs A,Busquets N,Erill I,Teixidó L,Barbé J

更新日期：2006-08-01 00:00:00

abstract：:Monoclonal antibodies to the matrix or E1 glycoprotein of mouse hepatitis virus (MHV) were tested for their ability to protect mice from a normally lethal challenge of MHV-4. Four antibodies were tested, and two of these, J.1.3 and J.3.9, were protective. Protection did not correlate with virus neutralization in vitro...

journal_title：Virology

authors： Fleming JO,Shubin RA,Sussman MA,Casteel N,Stohlman SA

更新日期：1989-01-01 00:00:00

abstract：:Mouse hepatitis virus strain A59 (MHV-A59) encodes within the 22-kb gene 1 a large polyprotein containing three proteinase domains with proven or predicted cysteine catalytic residues. E64d, a specific, irreversible inhibitor of cysteine (thiol) proteinases, inhibits the processing of the gene 1 polyprotein. Specifica...

journal_title：Virology

authors： Kim JC,Spence RA,Currier PF,Lu X,Denison MR

更新日期：1995-04-01 00:00:00

abstract：:Human herpesvirus 6 (HHV-6) is a ubiquitous betaherpesvirus that predominantly infects and replicates in CD4(+) T lymphocytes. However, the mechanism of HHV-6 transmission to T cells from the peripheral mucosa is unknown. Here we found that dendritic cells (DCs) can transmit HHV-6 to T cells, resulting in productive i...

journal_title：Virology

authors： Takemoto M,Imasawa T,Yamanishi K,Mori Y

更新日期：2009-03-15 00:00:00

abstract：:Sequence determinations of the hemagglutinin-neuraminidase (HN) glycoproteins of a temperature-sensitive mutant of Newcastle disease virus and two sequentially selected revertants had previously shown that substitution at a pair of residues, 129 and 175, resulted in a deficiency in neuraminidase (NA) activity, which w...

journal_title：Virology

authors： Sheehan JP,Iorio RM

更新日期：1992-08-01 00:00:00

abstract：:Hepatitis C virus (HCV) RNA replication requires viral nonstructural proteins as well as cellular factors. Recently, a cellular protein, synaptotagmin-binding, cytoplasmic RNA-interacting protein (SYNCRIP), also known as NSAP1, was found to bind HCV RNA and enhance HCV IRES-dependent translation. We investigate whethe...

journal_title：Virology

authors： Liu HM,Aizaki H,Choi KS,Machida K,Ou JJ,Lai MM

更新日期：2009-04-10 00:00:00

abstract：:Epstein-Barr virus (EBV) is a herpesvirus that transforms B-cells (B-LCL) and has undergone intense scrutiny owing to its association with Burkitt's lymphoma, nasopharyngeal carcinoma, and immunoblastic lymphomas. B-LCL have also proven useful in the study of human immunology. We describe a novel system for inducing e...

journal_title：Virology

authors： Curiel TJ,Cook DR,Bogedain C,Jilg W,Harrison GS,Cotten M,Curiel DT,Wagner E

更新日期：1994-02-01 00:00:00

abstract：:The phosphoprotein (P) of bovine respiratory syncytial virus (BRSV) is a multifunctional protein that plays a central role in transcription and replication of the viral genomic RNA. To investigate the domains and specific residues involved in different activities of the P protein, we generated a total of 22 deletion a...

journal_title：Virology

authors： Khattar SK,Yunus AS,Collins PL,Samal SK

更新日期：2001-07-05 00:00:00

abstract：:Assembly of the viral genome into virions is a critical process of the virus life cycle often defining the ability of the virus to move within the plant and to be transmitted horizontally to other plants. Closteroviridae virions are polar helical rods assembled primarily by a major coat protein, but with a related min...

journal_title：Virology

authors： Satyanarayana T,Gowda S,Mawassi M,Albiach-Martí MR,Ayllón MA,Robertson C,Garnsey SM,Dawson WO

更新日期：2000-12-05 00:00:00

abstract：:Feline morbillivirus (FmoPV) has recently been identified in Hong Kong and Japan. FmoPV is considered to belong to the genus Morbillivirus, in the family Paramyxoviridae. In this study, the complete nucleotide sequences of three strains of FmoPV detected in cats in Japan were determined. Among the six genes in FmoPV; ...

journal_title：Virology

authors： Park ES,Suzuki M,Kimura M,Maruyama K,Mizutani H,Saito R,Kubota N,Furuya T,Mizutani T,Imaoka K,Morikawa S

更新日期：2014-11-01 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) Rev is a 19-kDa regulatory protein which binds to unspliced and partially spliced HIV-1 RNAs. Export, splicing, stability, and translation of HIV-1 RNAs are influenced by Rev. To further understand the effect of Rev on HIV-1 RNA splicing, the intranuclear localization of uns...

journal_title：Virology

authors： Favaro JP,Borg KT,Arrigo SJ,Schmidt MG

更新日期：1998-09-30 00:00:00

abstract：:The orthopoxvirus serpin SPI-3 is N-glycosylated and suppresses fusion between infected cells. Although SPI-3 contains motifs conserved in inhibitory serpins, no proteinase inhibition by SPI-3 has been demonstrated, and mutations within the serpin reactive center loop (RCL) do not affect the ability to regulate cell f...

journal_title：Virology

authors： Turner PC,Baquero MT,Yuan S,Thoennes SR,Moyer RW

更新日期：2000-07-05 00:00:00

abstract：:Alpha human papillomaviruses (HPVs) are among the most common sexually transmitted agents of which a subset causes cervical neoplasia and cancer in humans. Alpha-PVs have also been identified in non-human primates although few studies have systematically characterized such types. We cloned and characterized 10 distinc...

journal_title：Virology

authors： Chen Z,van Doorslaer K,DeSalle R,Wood CE,Kaplan JR,Wagner JD,Burk RD

更新日期：2009-10-25 00:00:00

abstract：:Previous serological studies of experimental infection with duck hepatitis B virus (DHBV) have shown that the outcome of infection depends largely on the age of the duck at the time of inoculation. To examine the hypothesis that decreased susceptibility with increased age might be due to the loss of the virus receptor...

journal_title：Virology

authors： Qiao M,Gowans EJ,Burrell CJ

更新日期：1992-02-01 00:00:00

abstract：:West Nile virus (WNV) has become firmly established in northeastern US, reemerging every summer since its introduction into North America in 1999. To determine whether WNV overwinters locally or is reseeded annually, we examined the patterns of viral lineage persistence and replacement in Connecticut over 10 consecuti...

journal_title：Virology

authors： Armstrong PM,Vossbrinck CR,Andreadis TG,Anderson JF,Pesko KN,Newman RM,Lennon NJ,Birren BW,Ebel GD,Henn MR

更新日期：2011-08-15 00:00:00

abstract：:Two temperature-sensitive mutants of herpes simplex virus type 1 in complementation group 1-1 were analyzed to determine if the major DNA-binding protein they produced was thermolabile. Cells infected with these mutants were analyzed for deoxyribonucleoprotein complexes containing the DNA-binding protein. These comple...

journal_title：Virology

authors： Leinbach SS,Casto JF,Pickett TK

更新日期：1984-09-01 00:00:00

abstract：:In order to investigate the genome organization of the porcine epidemic diarrhea virus (PEDV) further, cDNA clones covering the region between the nucleocapsid and the spike (S) protein genes were independently constructed and sequenced for the two virulent isolates Br1/87 and CV777. Of the three major ORFs identified...

journal_title：Virology

authors： Duarte M,Tobler K,Bridgen A,Rasschaert D,Ackermann M,Laude H

更新日期：1994-02-01 00:00:00

abstract：:Five regions on the coat protein of BNYVV which had been shown previously to be involved in the formation of continuous epitopes were further analyzed by means of synthetic overlapping peptides. It was found that at least some of these regions may encompass several overlapping epitopes (or parts thereof). Four monoclo...

journal_title：Virology

authors： Commandeur U,Koenig R,Manteuffel R,Torrance L,Lüddecke P,Frank R

更新日期：1994-01-01 00:00:00

abstract：:Some retroviruses, including HIV-1, regulate the relative amounts of gag and pol gene products by a translational frameshift mechanism. The consequences of altering the ratios of the Gag and Pol proteins were tested using vaccinia virus expression vectors, in which the gag and pol genes were fused by placing them in t...

journal_title：Virology

authors： Karacostas V,Wolffe EJ,Nagashima K,Gonda MA,Moss B

更新日期：1993-04-01 00:00:00

abstract：:Hepatitis B virus (HBV) clone 4B replicated much more efficiently than clone 2A of the same genotype. Introduction of its T1753C, A1762T, G1764A, and C1766T core promoter mutations into the 2A genome greatly enhanced genome replication and suppressed HBeAg expression. Here we show that these effects are mediated by tr...

journal_title：Virology

authors： Tsai A,Kawai S,Kwei K,Gewaily D,Hutter A,Tong DR,Li J,Wands JR,Tong S

更新日期：2009-05-10 00:00:00

abstract：:A pseudorabies virus (PrV) mutant, deficient in the nonessential glycoprotein E (gE) and expressing the LacZ gene (gE- beta gal+ PrV), and its rescued virus were inoculated intranasally in mice. The median lethal dose of gE- beta gal+ PrV was similar to that of the parental Kaplan strain, but mice survived longer and ...

journal_title：Virology

authors： Babic N,Klupp B,Brack A,Mettenleiter TC,Ugolini G,Flamand A

更新日期：1996-05-01 00:00:00

abstract：:Monoclonal antibodies specific for eight antigenic sites on the G1 glycoprotein of La Crosse virus were studied in a kinetics of neutralization assay. When examined at the same protein concentration, there were differences in the rates and degree of neutralization induced by these antibodies. The maximum amount of neu...

journal_title：Virology

authors： Kingsford L

更新日期：1984-07-30 00:00:00

abstract：:Treatment of HeLa cells with human lymphoblastoid interferon (IFN-alpha) does not inhibit reovirus type 3 protein synthesis during virus infection. In contrast, reovirus translation is blocked by treatment of L cells with mouse IFN-alpha. The (2'-5')A synthetase activity is induced in HeLa cells by IFN-alpha treatment...

journal_title：Virology

authors： Feduchi E,Esteban M,Carrasco L

更新日期：1988-06-01 00:00:00

abstract：:Direct attenuation of West Nile (WN) virus strain NY99 for the purpose of vaccine development is not feasible due to its high virulence and pathogenicity. Instead, we created highly attenuated chimeric virus W1806 with the serological identity of NY99. To further attenuate W1806, we investigated effects of mutations f...

journal_title：Virology

authors： Yamshchikov V,Manuvakhova M,Rodriguez E

更新日期：2016-01-01 00:00:00