A single amino acid substitution in the hemagglutinin-neuraminidase of Newcastle disease virus results in a protein deficient in both functions.


:Sequence determinations of the hemagglutinin-neuraminidase (HN) glycoproteins of a temperature-sensitive mutant of Newcastle disease virus and two sequentially selected revertants had previously shown that substitution at a pair of residues, 129 and 175, resulted in a deficiency in neuraminidase (NA) activity, which was partially restored by a third substitution at residue 193. To evaluate the role of the substitution at residue 175 in diminished NA activity, the mutation was introduced into HN and the protein expressed in COS cells. The mutated HN not only had minimal NA activity but also was unable to absorb chicken erythrocytes, even though it was transported to the cell surface in normal amounts, in an apparently antigenic form. Attachment function was restored to the protein by the introduction of the additional substitution(s) at 129 and/or 193. These results indicate that residue 175 influences not only NA activity but also receptor recognition.






Sheehan JP,Iorio RM




Has Abstract


1992-08-01 00:00:00














  • Transcriptional activation by the E1A regions of adenovirus types 40 and 41.

    abstract::In order to establish whether the poor growth of the two fastidious adenoviruses types 40 and 41 (Ad40 and Ad41) in HeLa cells is due to a reduced trans-activation by the early region 1A (E1A), we have determined the trans-activating effect of this region on the expression of the chloramphenicol acetyltransferase (CAT...


    pub_type: 杂志文章


    authors: van Loon AE,Gilardi P,Perricaudet M,Rozijn TH,Sussenbach JS

    更新日期:1987-09-01 00:00:00

  • Activation of promoters for cellular lipogenic genes by hepatitis B virus large surface protein.

    abstract::Hepatitis B virus large surface protein has the unusual property of accumulating in a particulate form within a preGolgi compartment, leading to marked proliferation of intracellular membranes. We show here that large surface protein activates the promoters for two lipogenic genes that code for farnesyl diphosphate sy...


    pub_type: 杂志文章


    authors: Foo NC,Yen TS

    更新日期:2000-04-10 00:00:00

  • Finding our roots and celebrating our shoots: Plant virology in Virology, 1955-1964.

    abstract::To celebrate the sixtieth anniversary of Virology a survey is made of the plant viruses, virologists and their institutions, and tools and technology described in the first decade of plant virus publications in Virology. This was a period when plant viruses increasingly became tools of discovery as epistemic objects a...


    pub_type: 历史文章,杂志文章,评审


    authors: Scholthof KB

    更新日期:2015-05-01 00:00:00

  • Mutational analysis of the small intergenic region of maize streak virus.

    abstract::Maize streak virus (MSV) is a leafhopper-transmitted geminivirus containing one molecule of circular single-stranded DNA of about 2.7 kb. We have tested the infectivity of MSV mutants. A deletion of 29 bases in the small intergenic region (SIR) did not affect the infectivity of MSV. Mutants containing insertions of ol...


    pub_type: 杂志文章


    authors: Shen WH,Hohn B

    更新日期:1991-08-01 00:00:00

  • Raji B cells, misidentified as THP-1 cells, stimulate DC-SIGN-mediated HIV transmission.

    abstract::A number of studies examining interactions of dendritic cell (DC)-specific ICAM-3 grabbing nonintegrin (DC-SIGN) with viral pathogens have relied on monocytic transfectants as models for primary DCs. Here we show that the presumed "THP-1" monocytic cells used in these studies are instead Raji B cells. Moreover, we dem...


    pub_type: 杂志文章


    authors: Wu L,Martin TD,Carrington M,KewalRamani VN

    更新日期:2004-01-05 00:00:00

  • Receptor determinants of human and animal influenza virus isolates: differences in receptor specificity of the H3 hemagglutinin based on species of origin.

    abstract::The binding of influenza virus to erythrocytes and host cells is mediated by the interaction of the viral hemagglutinin (H) with cell surface receptors containing sialic acid (SA). The specificity of this interaction for 19 human and animal influenza isolates was examined using human erythrocytes enzymatically modifie...


    pub_type: 杂志文章


    authors: Rogers GN,Paulson JC

    更新日期:1983-06-01 00:00:00

  • Marburg virus vaccines based upon alphavirus replicons protect guinea pigs and nonhuman primates.

    abstract::Marburg virus (MBGV), for which no vaccines or treatments currently exist, causes an acute hemorrhagic fever with a high mortality rate in humans. We previously showed that immunization with either killed MBGV or a glycoprotein (GP) subunit prevented lethal infection in guinea pigs. In the studies reported here, an RN...


    pub_type: 杂志文章


    authors: Hevey M,Negley D,Pushko P,Smith J,Schmaljohn A

    更新日期:1998-11-10 00:00:00

  • Sequence diversity of the capsid gene and the nonstructural gene NS2B of dengue-3 virus in vivo.

    abstract::Previously, we studied the envelope (E) gene of dengue virus and reported that dengue-3 virus is present as a quasispecies. To investigate the extent of intrahost sequence variation of other dengue viral genes, we examined in this study the capsid (C) gene and the nonstructural gene, NS2B, derived directly from plasma...


    pub_type: 杂志文章


    authors: Wang WK,Sung TL,Lee CN,Lin TY,King CC

    更新日期:2002-11-10 00:00:00

  • Internalization and trafficking mechanisms of coxsackievirus B3 in HeLa cells.

    abstract::Coxsackievirus B3 (CVB3) is nonenveloped and has a single-stranded positive-sense RNA genome. CVB3 induces myocarditis and ultimately dilated cardiomyopathy. Although there are mounting evidences of an interaction between CVB3 particles and the cellular receptors, coxsackievirus and adenovirus receptor (CAR) and decay...


    pub_type: 杂志文章


    authors: Chung SK,Kim JY,Kim IB,Park SI,Paek KH,Nam JH

    更新日期:2005-03-01 00:00:00

  • Amino acid sequence analysis of bovine rotavirus B223 reveals a unique outer capsid protein VP4 and confirms a third bovine VP4 type.

    abstract::The nucleotide and deduced amino acid sequence of the gene 4 of bovine rotavirus strain B223 is described. The open reading frame is predicted to encode a VP4 of 772 amino acids, shorter than described for any other rotavirus strain sequenced to date. B223 VP4 shows 70 to 73% similarity to other rotavirus VP4 proteins...


    pub_type: 杂志文章


    authors: Hardy ME,Gorziglia M,Woode GN

    更新日期:1992-11-01 00:00:00

  • New highly divergent Plum pox virus isolates infecting sour cherry in Russia.

    abstract::Unusual Plum pox virus (PPV) isolates (named Tat isolates) were discovered on sour cherry (Prunus cerasus) in Russia. They failed to be recognized by RT-PCR using commonly employed primers specific to the strains C or CR (the only ones that proved able to infect sour cherry) as well as to the strains M and W. Some of ...


    pub_type: 杂志文章


    authors: Chirkov S,Ivanov P,Sheveleva A,Zakubanskiy A,Osipov G

    更新日期:2017-02-01 00:00:00

  • Anti-HIV activity of multibranched peptide constructs derived either from the cleavage sequence or from the transmembrane domain (gp41) of the human immunodeficiency virus type 1 envelope.

    abstract::Multibranched peptides (SPCs) derived either from the fusion protein (gp41) sequence or from the cleavage sequence of the human immunodeficiency virus type 1 envelope were chemically synthesized and tested for their ability to inhibit both syncytium formation and HIV production in CD4+ cells. The gp41-derived SPCs had...


    pub_type: 杂志文章


    authors: Sabatier JM,Mabrouk K,Moulard M,Rochat H,Van Rietschoten J,Fenouillet E

    更新日期:1996-09-15 00:00:00

  • Bovine aortic endothelial cells are susceptible to hantavirus infection; a new aspect in hantavirus ecology.

    abstract::Hantaviruses are enveloped RNA viruses that belong to the family Bunyaviridae. They are the causative agents of hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Hantaviruses show a worldwide distribution with specific rodent species as natural hosts. It is known that rodents can tr...


    pub_type: 杂志文章


    authors: Muranyi W,Kehm R,Bahr U,Müller S,Handermann M,Darai G,Zeier M

    更新日期:2004-01-05 00:00:00

  • Comparative analysis of the enveloped virions of two granulosis viruses of the armyworm, Pseudaletia unipuncta.

    abstract::The enveloped virions of two strains of a granulosis virus, a synergistic Hawaiian (GVH) and a nonsynergistic Oregonian (GVO) strain which infect the armyworm, Pseudaletia unipuncta, were liberated from their capsules with 0.02 N NaOH and purified by filtration through membrane filters. The envelopes were solubilized ...


    pub_type: 杂志文章


    authors: Yamamoto T,Tanada Y

    更新日期:1979-04-15 00:00:00

  • Hsp90 is involved in pseudorabies virus virion assembly via stabilizing major capsid protein VP5.

    abstract::Many viruses utilize molecular chaperone heat shock protein 90 (Hsp90) for protein folding and stabilization, however, the role of Hsp90 in herpesvirus lifecycle is obscure. Here, we provide evidence that Hsp90 participates in pseudorabies virus (PRV) replication. Viral growth kinetics assays show that Hsp90 inhibitor...


    pub_type: 杂志文章


    authors: Zhang WJ,Wang RQ,Li LT,Fu W,Chen HC,Liu ZF

    更新日期:2021-01-15 00:00:00

  • Equine herpesvirus 1 (EHV-1) glycoprotein M: effect of deletions of transmembrane domains.

    abstract::Equine herpesvirus 1 (EHV-1) recombinants that carry either a deletion of glycoprotein M (gM) or express mutant forms of gM were constructed. The recombinants were derived from strain Kentucky A (KyA), which also lacks genes encoding gE and gI. Plaques on RK13 cells induced by the gM-negative KyA were reduced in size ...


    pub_type: 杂志文章


    authors: Seyboldt C,Granzow H,Osterrieder N

    更新日期:2000-12-20 00:00:00

  • Effects of coat protein mutations and reduced movement protein expression on infection spread by cowpea chlorotic mottle virus and its hybrid derivatives.

    abstract::Previously we have reported that the essential 3a movement gene of icosahedral cowpea chlorotic mottle virus (CCMV) can be functionally replaced by the 30-kDa movement gene of rod-shaped sunn-hemp mosaic virus (SHMV). Because plant RNA viruses differ in requiring or not requiring coat protein for systemic infection, w...


    pub_type: 杂志文章


    authors: De Jong W,Mise K,Chu A,Ahlquist P

    更新日期:1997-05-26 00:00:00

  • C-terminal region of herpes simplex virus ICP8 protein needed for intranuclear localization.

    abstract::The herpes simplex virus single-stranded DNA-binding protein, ICP8, localizes initially to structures in the nucleus called prereplicative sites. As replication proceeds, these sites mature into large globular structures called replication compartments. The details of what signals or proteins are involved in the redis...


    pub_type: 杂志文章


    authors: Taylor TJ,Knipe DM

    更新日期:2003-05-10 00:00:00

  • Use of chimeric nectin-1(HveC)-related receptors to demonstrate that ability to bind alphaherpesvirus gD is not necessarily sufficient for viral entry.

    abstract::Human nectin-1 (HveC, Prr1), a member of the immunoglobulin superfamily and a receptor for the entry of herpes simplex viruses 1 and 2 (HSV-1, HSV-2), pseudorabies virus (PRV), and bovine herpesvirus 1 (BHV-1), binds to viral gD. For HSV-1, HSV-2, and PRV, the gD-binding region of nectin-1 has been localized to the N-...


    pub_type: 杂志文章


    authors: Geraghty RJ,Fridberg A,Krummenacher C,Cohen GH,Eisenberg RJ,Spear PG

    更新日期:2001-07-05 00:00:00

  • Identification and characterization of the repetitive DNA element in the genome of insect iridescent virus type 6.

    abstract::The genome of the Chilo iridescent virus (CIV) was analyzed for existence of repetitive DNA sequences by DNA-DNA hybridization using a defined and complete gene library of the viral genome (209 kbp) and by heteroduplex mapping. These experiments revealed the presence of repetitive DNA elements in the CIV genome, which...


    pub_type: 杂志文章


    authors: Fischer M,Schnitzler P,Delius H,Darai G

    更新日期:1988-12-01 00:00:00

  • No role for pepstatin-A-sensitive acidic proteinases in reovirus infections of L or MDCK cells.

    abstract::Strong evidence indicates that virions of mammalian reoviruses undergo proteolytic processing by acid-dependent cellular proteinases as an essential step in productive infection. Proteolytic processing takes the form of a series of cleavages of outer-capsid proteins final sigma3 and mu1/mu1C. Previous studies showed a...


    pub_type: 杂志文章


    authors: Kothandaraman S,Hebert MC,Raines RT,Nibert ML

    更新日期:1998-11-25 00:00:00

  • Selection of mutant CHO clones resistant to murine gammaherpesvirus 68 infection.

    abstract::Murine gammaherpesvirus 68 (MHV68) is used as a model to study gammaherpesvirus pathogenesis both in tissue culture systems and in vivo. We used a gene-trapping approach to get insight into cellular factors involved in MHV68 infection. By generating a library of gene-trapped CHO cells, we were able to isolate several ...


    pub_type: 杂志文章


    authors: Jarousse N,Coscoy L

    更新日期:2008-04-10 00:00:00

  • Towards quality and order in human papillomavirus research.

    abstract::The International Human Papillomavirus (HPV) Reference Center supports quality and order in HPV research and diagnostics. Notably, the center assigns HPV type numbers to novel HPV types, maintains a reference clone repository, and issues international proficiency panels for HPV genotyping. The established HPV types, c...


    pub_type: 杂志文章


    authors: Mühr LSA,Eklund C,Dillner J

    更新日期:2018-06-01 00:00:00

  • Characterization of a human astrovirus serotype 2 structural protein (VP26) that contains an epitope involved in virus neutralization.

    abstract::An improved purification procedure for human astrovirus serotype 2 (H-Ast2) has facilitated the isolation of a neutralizing monoclonal antibody (PL-2) directed against one of the major structural proteins (VP26) of H-Ast2. A minor component (VP29) of the virus particles is also recognized by PL-2 antibody. Immunofluor...


    pub_type: 杂志文章


    authors: Sanchez-Fauquier A,Carrascosa AL,Carrascosa JL,Otero A,Glass RI,Lopez JA,San Martin C,Melero JA

    更新日期:1994-06-01 00:00:00

  • Scavenger receptor-mediated Ad5 entry and acLDL accumulation in monocytes/macrophages synergistically trigger innate responses against viral infection.

    abstract::Adenovirus serotype 5 (Ad5) is a common cause of respiratory tract infection, and populations worldwide have high prevalence of anti-Ad5 antibodies, implying extensively prior infection. Ad5 infection potently activates the host innate defense and inflammation, but the molecular mechanisms are not completely clarified...


    pub_type: 杂志文章


    authors: Li P,Feng F,Pan E,Fan X,Yang Q,Guan M,Chen L,Sun C

    更新日期:2018-06-01 00:00:00

  • Analysis of nucleotides 13-96 of the human parainfluenza virus type 3 antigenomic promoter reveals positive- and negative-acting replication elements.

    abstract::During replication of human parainfluenza virus type 3 (HPIV3), the 96-nucleotide antigenomic promoter (AGP) of HPIV3 directs the synthesis of genomic RNA. Previous work showed that nucleotides 1-12 were critical in promoting replication of an HPIV3 minireplicon, but nucleotides 13-96 were not investigated. In this st...


    pub_type: 杂志文章


    authors: Gander JR,Schwan LM,Hoffman MA

    更新日期:2011-10-25 00:00:00

  • Unsupervised learning techniques reveal heterogeneity in memory CD8+ T cell differentiation following acute, chronic and latent viral infections.

    abstract::CD8+ T lymphocytes are critical for the control of gammaherpesvirus latency. To determine how memory CD8+ T cells generated during latency differ from those primed during acute or chronic viral infection, we adoptively transferred naive P14 CD8+ T cells into uninfected recipients, and examined surface proteins, cytoki...


    pub_type: 杂志文章


    authors: Liu M,Barton ES,Jennings RN,Oldenburg DG,Whirry JM,White DW,Grayson JM

    更新日期:2017-09-01 00:00:00

  • Proteolytic maturation of the 206-kDa nonstructural protein encoded by turnip yellow mosaic virus RNA.

    abstract::The longest open reading frame of turnip yellow mosaic virus genomic RNA (ORF-206) encodes a 206-kDa nonstructural protein. The most prominent in vitro translation products of ORF-206 are the full-length p206 and a shorter N-coterminal 150-kDa protein. We have confirmed these assignments by immunoprecipitation of in v...


    pub_type: 杂志文章


    authors: Bransom KL,Weiland JJ,Dreher TW

    更新日期:1991-09-01 00:00:00

  • Mapping nucleotides in the 126-kDa protein gene that control the differential symptoms induced by two strains of tobacco mosaic virus.

    abstract::The differential symptom determinants of the Holmes' masked (M) and U1 strains of tobacco mosaic virus previously were mapped to the 5'-coterminal open reading frame (ORF) encoding the 126-kDa protein and the N-terminal two-thirds of the 183-kDa protein. Both proteins influence viral RNA accumulation, but the function...


    pub_type: 杂志文章


    authors: Shintaku MH,Carter SA,Bao Y,Nelson RS

    更新日期:1996-07-01 00:00:00

  • Herpes simplex virus 1 infection is required to produce ICP27 immunoreactive triplet forms when ribosomal aminoacyl-tRNA translocation is blocked by cycloheximide.

    abstract::Infected cell protein (ICP) 27 is an essential herpes simplex virus type 1 (HSV-1) phosphoprotein required for optimal viral DNA and early or late gene synthesis. Three slow-migrating immunoreactive species were detected using multiple anti-ICP27 antibodies following HSV-1 infection of HEp-2 and Vero cells in the pres...


    pub_type: 杂志文章


    authors: Sanfilippo CM,Lombardozzi RC,Chirimuuta FN,Blaho JA

    更新日期:2004-07-01 00:00:00