Selection of mutant CHO clones resistant to murine gammaherpesvirus 68 infection.


:Murine gammaherpesvirus 68 (MHV68) is used as a model to study gammaherpesvirus pathogenesis both in tissue culture systems and in vivo. We used a gene-trapping approach to get insight into cellular factors involved in MHV68 infection. By generating a library of gene-trapped CHO cells, we were able to isolate several clones that exhibited various degrees of resistance to MHV68-induced cytopathic effect. Clones that showed the highest degree of resistance were affected at the early stage of the viral cycle, with the vast majority of these clones being deficient for heparan sulfate (HS) expression at the cell surface. Heparan sulfate expression could be restored in all the HS-deficient clones by expression of EXT1, an enzyme that is essential for the biosynthesis of HS. Consistent with the role of HS in viral entry, HS-deficient CHO cells did not support viral internalization. Cell surface heparan sulfate proteoglycans (HSPG) are mostly composed of HS chains attached to two families of core proteins, the transmembrane syndecans and the GPI-anchored glypicans. Treatment of CHO cells with phosphatidylinositol-specific phospholipase C (PI-PLC) did not significantly affect the level of HS expression, indicating that the glypicans are not a major source of HSPG in CHO cells. By contrast, treatment of CHO cells with PMA, a drug known to accelerate syndecan shedding, resulted in a decrease in both HS expression and susceptibility to MHV68; these effects were abolished by TIMP-3, a specific inhibitor of syndecan shedding. All together, our results confirm the essential role of HS in MHV68 infection and identify the syndecans as a major source of HSPG used by the virus as coreceptors to infect CHO cells.






Jarousse N,Coscoy L




Has Abstract


2008-04-10 00:00:00
















  • Location, size, and complexity of epitopes on the coat protein of beet necrotic yellow vein virus studied by means of synthetic overlapping peptides.

    abstract::Five regions on the coat protein of BNYVV which had been shown previously to be involved in the formation of continuous epitopes were further analyzed by means of synthetic overlapping peptides. It was found that at least some of these regions may encompass several overlapping epitopes (or parts thereof). Four monoclo...


    pub_type: 杂志文章


    authors: Commandeur U,Koenig R,Manteuffel R,Torrance L,Lüddecke P,Frank R

    更新日期:1994-01-01 00:00:00

  • Localization of a VP3 epitope of infectious bursal disease virus.

    abstract::An immunodominant region of VP3, one of the two structural proteins of infectious bursal disease virus (IBDV strain 002-73), has been mapped by restriction site-specific deletion analysis and subcloning in Escherichia coli, followed by immunoblot analysis of the synthesized products. The epitope located within 58 amin...


    pub_type: 杂志文章


    authors: Jagadish MN,Azad AA

    更新日期:1991-10-01 00:00:00

  • Mutational analysis of early region 4 of bovine adenovirus type 3.

    abstract::The primary objective of characterizing bovine adenovirus type 3 (BAV3) in greater detail is to develop it as a vector for gene therapy and vaccination of humans and animals. A series of BAV3 early region 4 (E4) deletion-mutant viruses, containing deletions in individual E4 open reading frames (Orf) or combinations of...


    pub_type: 杂志文章


    authors: Baxi MK,Robertson J,Babiuk LA,Tikoo SK

    更新日期:2001-11-10 00:00:00

  • Inhibition of host cellular ribonucleic acid synthesis by glycoprotein of mumps virus.

    abstract::After infection with mumps virus, cellular ribonucleic acid synthesis of a murine lymphoma cell line, EL4, was appreciably depressed. The inactivation of viral infectivity by ultraviolet irradiation or the treatment of cells with mouse interferon did not abolish the inhibiting effect, suggesting that virus replication...


    pub_type: 杂志文章


    authors: Yamada A,Tsurudome M,Hishiyama M,Ito Y

    更新日期:1984-06-01 00:00:00

  • Rescue of infectivity by sequential in vitro transcapsidation of rotavirus core particles with inner capsid and outer capsid proteins.

    abstract::We recently developed an in vitro transcapsidation system in which infectivity of single-shelled (ss) rotavirus particles was successfully rescued (Chen and Ramig, Virology [1993]). Here, we report the rescue of infectivity of rotavirus core particles using virus strain B223 (G serotype 10) as the core donor and strai...


    pub_type: 杂志文章


    authors: Chen D,Ramig RF

    更新日期:1993-06-01 00:00:00

  • Therapeutic immunisation with COPV early genes by epithelial DNA delivery.

    abstract::Following challenge with COPV (canine oral papillomavirus), DNA plasmids encoding COPV L1, E1 or E2 protein were delivered into oral mucosal and cutaneous sites in beagles using particle-mediated immunotherapeutic delivery (PMID). Two weeks post-challenge, a priming dose of 8 microg DNA was delivered followed by a boo...


    pub_type: 杂志文章


    authors: Moore RA,Walcott S,White KL,Anderson DM,Jain S,Lloyd A,Topley P,Thomsen L,Gough GW,Stanley MA

    更新日期:2003-09-30 00:00:00

  • Characterization of hepatitis A virus structural proteins.

    abstract::HAV particles isolated from infected cells banded at buoyant densities of 1.42, 1.32, and 1.20 g/ml, and distinctive protein patterns were established by gel electrophoresis and reverse phase high performance liquid chromatography. The relatively higher amounts of p30 in particles with lower buoyant densities suggest ...


    pub_type: 杂志文章


    authors: Gauss-Müller V,Lottspeich F,Deinhardt F

    更新日期:1986-12-01 00:00:00

  • Gene expression of the human Torque Teno Virus isolate P/1C1.

    abstract::Torque Teno Virus (TTV) has been assigned to the floating genus Anellovirus. TTV ssDNA genomes have a size of 3.6 to 3.8 kb and display up to 30% nucleotide diversity. The pathogenic potential of TTV is under investigation. To address a putative link of pathogenicity with the observed sequence variations, the transcri...


    pub_type: 杂志文章


    authors: Mueller B,Maerz A,Doberstein K,Finsterbusch T,Mankertz A

    更新日期:2008-11-10 00:00:00

  • Destabilization of the RB tumor suppressor protein and stabilization of p53 contribute to HPV type 16 E7-induced apoptosis.

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    pub_type: 杂志文章


    authors: Jones DL,Thompson DA,Münger K

    更新日期:1997-12-08 00:00:00

  • High-affinity Rep-binding is not required for the replication of a geminivirus DNA and its satellite.

    abstract::The 682-nt satellite DNA (sat-DNA) of Tomato leaf curl virus (TLCV) depends on the helper virus for its replication. In contrast to the strict specificity that exists in each geminivirus for its cognate replication associated protein (Rep), TLCV sat-DNA can utilize Rep encoded by distinct geminiviruses. We have used a...


    pub_type: 杂志文章


    authors: Lin B,Akbar Behjatnia SA,Dry IB,Randles JW,Rezaian MA

    更新日期:2003-01-20 00:00:00

  • Deep sequencing of H7N8 avian influenza viruses from surveillance zone supports H7N8 high pathogenicity avian influenza was limited to a single outbreak farm in Indiana during 2016.

    abstract::In mid-January 2016, an outbreak of H7N8 high-pathogenicity avian influenza virus (HPAIV) in commercial turkeys occurred in Indiana. Surveillance within the 10km control zone identified H7N8 low-pathogenicity avian influenza virus (LPAIV) in nine surrounding turkey flocks but no other HPAIV-affected premises. We seque...


    pub_type: 杂志文章


    authors: Lee DH,Torchetti MK,Killian ML,Swayne DE

    更新日期:2017-07-01 00:00:00

  • Identification of genetic determinants of a tick-borne flavivirus associated with host-specific adaptation and pathogenicity.

    abstract::Tick-borne flaviviruses are maintained in nature in an enzootic cycle involving a tick vector and a vertebrate host. Thus, the virus replicates in two disparate hosts, each providing selective pressures that can influence virus replication and pathogenicity. To identify viral determinants associated with replication i...


    pub_type: 杂志文章


    authors: Mitzel DN,Best SM,Masnick MF,Porcella SF,Wolfinbarger JB,Bloom ME

    更新日期:2008-11-25 00:00:00

  • Isolation of a Friend recombinant polytropic virus with a T-cell-restricted leukemogenicity.

    abstract::Hematopoietic malignant cells of various types were isolated from ICFW mice inoculated as newborn or adult with F-MuLV and grafted into recipient mice. After repeated in vivo cell transplants, several recombinant polytropic viruses (also termed MCF or dualtropic viruses) were isolated from tumors by limiting dilution....


    pub_type: 杂志文章


    authors: Della-Valle V,Wendling F,Sitbon M,Varlet P,Mathieu-Mahul D,Larsen CJ

    更新日期:1990-10-01 00:00:00

  • Glycosylation of the cauliflower mosaic virus capsid polypeptide.

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    pub_type: 杂志文章


    authors: Du Plessis DH,Smith P

    更新日期:1981-03-01 00:00:00

  • A C-terminal deletion mutant of pokeweed antiviral protein inhibits programmed +1 ribosomal frameshifting and Ty1 retrotransposition without depurinating the sarcin/ricin loop of rRNA.

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    pub_type: 杂志文章


    authors: Hudak KA,Hammell AB,Yasenchak J,Tumer NE,Dinman JD

    更新日期:2001-01-05 00:00:00

  • Reduced levels of alpha1(XI) procollagen mRNA in SV40-transformed cells.

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    pub_type: 杂志文章


    authors: Sompayrac L,Jane S,Danna KJ

    更新日期:1996-04-15 00:00:00

  • The M4 gene of gammaHV68 encodes a secreted glycoprotein and is required for the efficient establishment of splenic latency.

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    pub_type: 杂志文章


    authors: Evans AG,Moorman NJ,Willer DO,Speck SH

    更新日期:2006-01-20 00:00:00

  • Characterization of incompletely typed rotavirus strains from Guinea-Bissau: identification of G8 and G9 types and a high frequency of mixed infections.

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    pub_type: 杂志文章


    authors: Fischer TK,Page NA,Griffin DD,Eugen-Olsen J,Pedersen AG,Valentiner-Branth P,Mølbak K,Sommerfelt H,Nielsen NM

    更新日期:2003-06-20 00:00:00

  • Deletion of glycoprotein gE reduces the propagation of pseudorabies virus in the nervous system of mice after intranasal inoculation.

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    pub_type: 杂志文章


    authors: Babic N,Klupp B,Brack A,Mettenleiter TC,Ugolini G,Flamand A

    更新日期:1996-05-01 00:00:00

  • The spiroplasma virus 4 replicative form cloned in Escherichia coli transfects spiroplasmas.

    abstract::The replicative form (RF) of spiroplasma virus 4 (SpV4) has been purified from infected cells of Spiroplasma melliferum strain G1 by alkaline lysis followed by low melting point agarose gel electrophoresis. A partial restriction map has been established. The circular RF was linearized by cutting at the unique ClaI res...


    pub_type: 杂志文章


    authors: Pascarel-Devilder MC,Renaudin J,Bove JM

    更新日期:1986-06-01 00:00:00

  • The AG recombinant IbNG and novel strains of group M HIV-1 are common in Cameroon.

    abstract::The genetic diversity of group M HIV-1 is highest in west central Africa. Blood samples from four locations in Cameroon were collected to determine the molecular epidemiology of HIV-1. The C2-V5 region of envelope was sequenced from 39 of the 40 samples collected, and 7 samples were sequenced across the genome. All st...


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    authors: Carr JK,Torimiro JN,Wolfe ND,Eitel MN,Kim B,Sanders-Buell E,Jagodzinski LL,Gotte D,Burke DS,Birx DL,McCutchan FE

    更新日期:2001-07-20 00:00:00

  • Impaired entry of soluble receptor-resistant mutants of mouse hepatitis virus into cells expressing MHVR2 receptor.

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    pub_type: 杂志文章


    authors: Matsuyama S,Taguchi F

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  • A role for glycoprotein C in pseudorabies virus entry that is independent of virus attachment to heparan sulfate and which involves the actin cytoskeleton.

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    pub_type: 杂志文章


    authors: Rue CA,Ryan P

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    pub_type: 杂志文章


    authors: Julander JG,Siddharthan V,Blatt LM,Schafer K,Sidwell RW,Morrey JD

    更新日期:2007-04-10 00:00:00

  • Functional and molecular characterization of African swine fever virus mutants resistant to phosphonoacetic acid.

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    pub_type: 杂志文章


    authors: Marques MI,Costa JV

    更新日期:1995-12-01 00:00:00

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    pub_type: 杂志文章


    authors: Luckow VA,Summers MD

    更新日期:1989-05-01 00:00:00

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    pub_type: 杂志文章


    authors: Mackenzie JM,Khromykh AA,Jones MK,Westaway EG

    更新日期:1998-06-05 00:00:00

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    pub_type: 杂志文章


    authors: Caron M,Besson G,Etenna SL,Mintsa-Ndong A,Mourtas S,Radaelli A,Morghen Cde G,Loddo R,La Colla P,Antimisiaris SG,Kazanji M

    更新日期:2010-09-15 00:00:00

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    pub_type: 杂志文章


    authors: Jones RW,Jackson AO

    更新日期:1990-12-01 00:00:00

  • Cytoplasmic tail domain of glycoprotein B is essential for HHV-6 infection.

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    pub_type: 杂志文章


    authors: Mahmoud NF,Jasirwan C,Kanemoto S,Wakata A,Wang B,Hata Y,Nagamata S,Kawabata A,Tang H,Mori Y

    更新日期:2016-03-01 00:00:00